BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0083.p (487 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8ISI3.1 D8ISI3_HERSS Flagellar biosynthesis GTP-binding protein 695 0.0 F1VXQ5.1 F1VXQ5_9BURK Flagellar biosynthesis protein FlhF 585 e-165 A6SZS8.1 A6SZS8_JANMA FlhF flagellar biosynthesis protein 584 e-164 A4G4P8.1 A4G4P8_HERAR Putative flagellar biosynthesis regulator ... 541 e-152 E4QKP1.1 E4QKP1_METS6 GTP-binding signal recognition particle SR... 468 e-130 >D8ISI3.1 D8ISI3_HERSS Flagellar biosynthesis GTP-binding protein Length = 494 Score = 695 bits (1793), Expect = 0.0, Method: Compositional matrix adjust. Identities = 361/504 (71%), Positives = 398/504 (78%), Gaps = 27/504 (5%) Query: 1 MNVKKFIAGSSRDAWRQVREALGPDAVILSNRNIDDGVEILAMANEDMSSLVVPAMSKDA 60 MNVKKFIA SSR+AWRQVREALGPDAVILSNRNI DGVEILAMANEDM++LV P ++D Sbjct: 1 MNVKKFIANSSREAWRQVREALGPDAVILSNRNIPDGVEILAMANEDMTTLVAPTGARDP 60 Query: 61 VPRPAVRR-------PPAPQQSLQAAVPRTRVEQQAQSPGASMPVEHNTAQADA------ 107 RP PPA Q A + + + GA + + + ++ A Sbjct: 61 SGRPQAASLQQQSGLPPAQQPRRPALLSSPPAQARPAREGAPLLDQPGSERSQARAEAAP 120 Query: 108 ----RVWRTRNAARASAKEQQAQALTPAQVAAGPKAAAASAAAVRPQQAGVMAEFDGKDY 163 RVWR+R R S Q+ A + RP+ A + EFDGKDY Sbjct: 121 AADARVWRSRT--RDSGNAQRRAAAPAGAPQGQEQEQD------RPRSA--LPEFDGKDY 170 Query: 164 DEILSDVMSEIRSMRGVLETQLAEISWGGTQKREPAKAAIMREMLAAGFSASLSRLITEN 223 DEILS+VMSEIRSMRGVLETQLAEISWGGTQKREP K +++EMLAAGFSASLSRLITEN Sbjct: 171 DEILSEVMSEIRSMRGVLETQLAEISWGGTQKREPLKGIVLKEMLAAGFSASLSRLITEN 230 Query: 224 LPQSPKSQDAMFWVKSVLAKNLMTMANENELLEEGGVYALVGPTGVGKTTTTAKLAARCV 283 LP + KSQD MFWVKSVLA+NL T+ANENELLE GGV+A+VGPTGVGKTTTTAKLAARCV Sbjct: 231 LPANSKSQDIMFWVKSVLARNLNTLANENELLENGGVFAMVGPTGVGKTTTTAKLAARCV 290 Query: 284 MRHGPGKLALITTDGYRIGGYEQLRIYGKILGVMVHSVKDESDLRIALDELRGKHTVLID 343 MRHG GKLALITTDGYRIGGYEQLRIYGKILGVMVHSVKDE+DLRIAL+EL+GKHTVLID Sbjct: 291 MRHGSGKLALITTDGYRIGGYEQLRIYGKILGVMVHSVKDETDLRIALEELKGKHTVLID 350 Query: 344 TAGMGQRDQMVAEQAAMLSGAGTDIKRLLCLNATATGGTLNEVVSAYSGDGLAGCIITKL 403 TAG+GQRDQMVAEQ AML+GAG DIKRLLCLNATATGGTLNEVV AYS GLAGCIITKL Sbjct: 351 TAGVGQRDQMVAEQDAMLTGAGVDIKRLLCLNATATGGTLNEVVHAYSSSGLAGCIITKL 410 Query: 404 DEAATIGNVLDVVIRQKLNLYYVANGQRVPEDLHVANRLYLADRAFKLKRETAPFEFQDA 463 DEAATIGNVLDVVIR KLNL+YVANGQRVPEDLHVAN+LYLADRAFK KRETAPFEFQ+ Sbjct: 411 DEAATIGNVLDVVIRHKLNLHYVANGQRVPEDLHVANKLYLADRAFKQKRETAPFEFQEG 470 Query: 464 ELPVVVGPTATAMNDKSLREVILG 487 ELP V+GPTA +MNDK LREV G Sbjct: 471 ELPGVIGPTAMSMNDKGLREVSFG 494 >F1VXQ5.1 F1VXQ5_9BURK Flagellar biosynthesis protein FlhF Length = 443 Score = 585 bits (1509), Expect = e-165, Method: Compositional matrix adjust. Identities = 302/491 (61%), Positives = 364/491 (74%), Gaps = 52/491 (10%) Query: 1 MNVKKFIAGSSRDAWRQVREALGPDAVILSNRNIDDGVEILAMANEDMSSLVVPAMSKDA 60 MNVKKF A SSRDA RQVREALGPDAVILSNRNI+ VEILA+A+EDMSSL P + K+ Sbjct: 1 MNVKKFSASSSRDALRQVREALGPDAVILSNRNINGQVEILALASEDMSSLAAPVIEKET 60 Query: 61 -VPRPAVRRPPAPQQSLQAAVPRTRVEQQA---QSPGASMPVEHNTAQADARVWRTRNAA 116 + + A+ PA + ++++A + R E +A Q+ G + +E + DARV Sbjct: 61 LIAQVAL---PAQEATVRSAFAKRRTEFKADTDQASGLAGALEQSQQSRDARV------- 110 Query: 117 RASAKEQQAQALTPAQVAAGPKAAAASAAAVRPQQAGVMAEFDGKDYDEILSDVMSEIRS 176 P+ AA + V+ E ++++M+E+RS Sbjct: 111 --------------------PEPAAPIS---------VLRE---------MAEMMNELRS 132 Query: 177 MRGVLETQLAEISWGGTQKREPAKAAIMREMLAAGFSASLSRLITENLPQSPKSQDAMFW 236 MR ++E+Q +E+SW QKREP KA I+RE+LAAGFSASLSR + ENLP + M W Sbjct: 133 MRTMMESQFSELSWSSQQKREPVKAVILRELLAAGFSASLSRYLIENLPAGGTAAAGMSW 192 Query: 237 VKSVLAKNLMTMANENELLEEGGVYALVGPTGVGKTTTTAKLAARCVMRHGPGKLALITT 296 +K+V+A+N+ + +E+ +LE+GGV+ALVGPTGVGKTTTTAKLAARCVMRHGPGKLALI T Sbjct: 193 IKTVMARNINAITDESAMLEQGGVFALVGPTGVGKTTTTAKLAARCVMRHGPGKLALIPT 252 Query: 297 DGYRIGGYEQLRIYGKILGVMVHSVKDESDLRIALDELRGKHTVLIDTAGMGQRDQMVAE 356 DGYRIGGYEQLRIYGKILGVMVHSVKDESDLRIALDEL+ KHTVLIDT G+ QRD MV E Sbjct: 253 DGYRIGGYEQLRIYGKILGVMVHSVKDESDLRIALDELKNKHTVLIDTVGVSQRDHMVTE 312 Query: 357 QAAMLSGAGTDIKRLLCLNATATGGTLNEVVSAYSGDGLAGCIITKLDEAATIGNVLDVV 416 Q AML GAG+ +KRLLCLNAT+TG TLNEVV AY G GLAGC++TKLDEAATIG+VLDVV Sbjct: 313 QVAMLQGAGSRVKRLLCLNATSTGETLNEVVRAYQGSGLAGCVMTKLDEAATIGSVLDVV 372 Query: 417 IRQKLNLYYVANGQRVPEDLHVANRLYLADRAFKLKRETAPFEFQDAELPVVVGPTATAM 476 IRQKLNL+YVANGQRVPEDLHVAN+ YL DRAFKLKRE+ PF+F DAELP+V+G T A Sbjct: 373 IRQKLNLFYVANGQRVPEDLHVANKQYLVDRAFKLKRESKPFQFTDAELPLVMGGTTRAS 432 Query: 477 NDKSLREVILG 487 NDKSLREV LG Sbjct: 433 NDKSLREVHLG 443 >A6SZS8.1 A6SZS8_JANMA FlhF flagellar biosynthesis protein Length = 421 Score = 584 bits (1506), Expect = e-164, Method: Compositional matrix adjust. Identities = 302/487 (62%), Positives = 349/487 (71%), Gaps = 66/487 (13%) Query: 1 MNVKKFIAGSSRDAWRQVREALGPDAVILSNRNIDDGVEILAMANEDMSSLVVPAMSKDA 60 MNV+KF A +SR AW VREALGPDAVILSNR ID GVEILA+A EDM+SL Sbjct: 1 MNVRKFTASTSRGAWSLVREALGPDAVILSNRAIDGGVEILALAGEDMASLA-------- 52 Query: 61 VPRPAVRRPPAPQQSLQAAVPRTRVEQQAQSPGASMPVEHNTAQADARVWRTRNAARASA 120 P V R + +L + VP H+ A + T NAA++S Sbjct: 53 --EPMVERAAISETTLASLVP------------------HHKPSQPAPLALTSNAAQSSD 92 Query: 121 KEQQAQALTPAQVAAGPKAAAASAAAVRPQQAGVMAEFDGKDYDEILSDVMSEIRSMRGV 180 K + + A L+++MSEIRSMRG+ Sbjct: 93 KVEISSAE--------------------------------------LNNMMSEIRSMRGM 114 Query: 181 LETQLAEISWGGTQKREPAKAAIMREMLAAGFSASLSRLITENLPQSPKSQDAMFWVKSV 240 LE QL+E++W QKREP K+ ++REMLAAGFSASL+R + LP +Q M W+KS+ Sbjct: 115 LEIQLSELAWNDPQKREPVKSTVLREMLAAGFSASLARYLIARLPAGASNQSGMEWIKSI 174 Query: 241 LAKNLMTMANENELLEEGGVYALVGPTGVGKTTTTAKLAARCVMRHGPGKLALITTDGYR 300 LA+NL T+ +ENE+LE+GGVYALVGPTGVGKTTTTAKLAARCVMRHGP KLALITTDGYR Sbjct: 175 LARNLATIGDENEILEKGGVYALVGPTGVGKTTTTAKLAARCVMRHGPSKLALITTDGYR 234 Query: 301 IGGYEQLRIYGKILGVMVHSVKDESDLRIALDELRGKHTVLIDTAGMGQRDQMVAEQAAM 360 IGGYEQLRIYGKILGVMVHSVKDE+DLRIALDEL+GKHTVLIDT G+ QRDQMVA+Q AM Sbjct: 235 IGGYEQLRIYGKILGVMVHSVKDEADLRIALDELKGKHTVLIDTVGVSQRDQMVADQIAM 294 Query: 361 LSGAGTDIKRLLCLNATATGGTLNEVVSAYSGDGLAGCIITKLDEAATIGNVLDVVIRQK 420 L+GAG +KRLLCLNAT+TG TLNEVV +Y GDGLAGCI+TKLDEAATIG+ LDVVIRQK Sbjct: 295 LTGAGDQVKRLLCLNATSTGETLNEVVRSYMGDGLAGCILTKLDEAATIGSALDVVIRQK 354 Query: 421 LNLYYVANGQRVPEDLHVANRLYLADRAFKLKRETAPFEFQDAELPVVVGPTATAMNDKS 480 LNLYYVANGQRVPEDLHVAN+ YL DRAFKLKRETAPF+ DAE+P V+ T MNDKS Sbjct: 355 LNLYYVANGQRVPEDLHVANQQYLVDRAFKLKRETAPFQLGDAEIPAVMANTVRGMNDKS 414 Query: 481 LREVILG 487 LREVILG Sbjct: 415 LREVILG 421 >A4G4P8.1 A4G4P8_HERAR Putative flagellar biosynthesis regulator FlhF Length = 423 Score = 541 bits (1395), Expect = e-152, Method: Compositional matrix adjust. Identities = 254/321 (79%), Positives = 285/321 (88%) Query: 167 LSDVMSEIRSMRGVLETQLAEISWGGTQKREPAKAAIMREMLAAGFSASLSRLITENLPQ 226 L VMSEIRSMRG+LE QLAE++W KREPAK++++REMLAAGFSASL+R +TE +P Sbjct: 103 LHSVMSEIRSMRGMLEMQLAELAWTDQHKREPAKSSVLREMLAAGFSASLARYLTERMPA 162 Query: 227 SPKSQDAMFWVKSVLAKNLMTMANENELLEEGGVYALVGPTGVGKTTTTAKLAARCVMRH 286 Q M W+K++L +NL T+ NENE+LE+GGVYALVGPTGVGKTTTTAKLAARCVMRH Sbjct: 163 GASKQSGMDWIKAILTRNLATIGNENEILEKGGVYALVGPTGVGKTTTTAKLAARCVMRH 222 Query: 287 GPGKLALITTDGYRIGGYEQLRIYGKILGVMVHSVKDESDLRIALDELRGKHTVLIDTAG 346 GPGKLALITTDGYRIGGYEQLRIYGKILGVMVHSVKDE+DLRIALDEL+GKHTVLIDT G Sbjct: 223 GPGKLALITTDGYRIGGYEQLRIYGKILGVMVHSVKDEADLRIALDELKGKHTVLIDTVG 282 Query: 347 MGQRDQMVAEQAAMLSGAGTDIKRLLCLNATATGGTLNEVVSAYSGDGLAGCIITKLDEA 406 + QRD MVAEQ AMLSGAG+ +KRLLCLNAT+TG TLNEV +Y GDGLAGCI+TKLDEA Sbjct: 283 VSQRDNMVAEQIAMLSGAGSQVKRLLCLNATSTGETLNEVARSYMGDGLAGCILTKLDEA 342 Query: 407 ATIGNVLDVVIRQKLNLYYVANGQRVPEDLHVANRLYLADRAFKLKRETAPFEFQDAELP 466 ATIG+ LDVVIRQKLNLYYVANGQRVPEDLHVAN+ YL DRAFKLKRETAPF+F DAE+P Sbjct: 343 ATIGSALDVVIRQKLNLYYVANGQRVPEDLHVANQQYLVDRAFKLKRETAPFQFSDAEIP 402 Query: 467 VVVGPTATAMNDKSLREVILG 487 +V+ TA AMNDKSLREVILG Sbjct: 403 LVMANTAKAMNDKSLREVILG 423 Score = 82.8 bits (203), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 39/58 (67%), Positives = 46/58 (79%) Query: 1 MNVKKFIAGSSRDAWRQVREALGPDAVILSNRNIDDGVEILAMANEDMSSLVVPAMSK 58 MNV+KF A +SR AW VREALGPDAVILSNR ++ GVEILA+A EDM+SL P + K Sbjct: 1 MNVRKFTASTSRGAWSLVREALGPDAVILSNRAVEGGVEILALAGEDMASLAEPMVEK 58 >E4QKP1.1 E4QKP1_METS6 GTP-binding signal recognition particle SRP54 G-domain protein Length = 451 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 252/475 (53%), Positives = 320/475 (67%), Gaps = 39/475 (8%) Query: 1 MNVKKFIAGSSRDAWRQVREALGPDAVILSNRNIDDGVEILAMANEDMSSLVVPAMSKDA 60 MN+K+F A SSR+A VR++LG DAVILSNR ++ G EILA EDM+S + D Sbjct: 1 MNIKRFFASSSREALAMVRKSLGEDAVILSNRAVNGGNEILAFKEEDMASFM-----SDD 55 Query: 61 VPRPAVRRPPAPQQSLQAAVPRTRVEQQAQSPGASMPVEHNTAQADA-RVWRTRNAARAS 119 P + +R AP A P + + + A PV +A+ R RT A + Sbjct: 56 TPVQSSKRRAAP-----AMEPLQAAQARPAARQARPPVRQAAPEAEPYRAVRTEPAPTTT 110 Query: 120 AKEQQAQALTPAQVAAGPKAAAASAAAVRPQQAGVMAEFDGKDYDEILSDVMSEIRSMRG 179 A+ PK A A PQ ++ ++D+++EIR+MR Sbjct: 111 AR---------------PKVTTTPAQAAAPQS------------NKQINDMLNEIRNMRS 143 Query: 180 VLETQLAEISWGGTQKREPAKAAIMREMLAAGFSASLSRLITENLPQSPKSQDAMFWVKS 239 V+E+QL EISWG Q R+P K+ + +L+AGFSA+LSR I EN+P S +A WV+S Sbjct: 144 VIESQLVEISWGNIQHRDPVKSNTLNMLLSAGFSAALSRQIAENMPDHRTSAEANTWVQS 203 Query: 240 VLAKNLMTMANENELLEEGGVYALVGPTGVGKTTTTAKLAARCVMRHGPGKLALITTDGY 299 +L KNL + NE+E+L GGV+AL+GPTGVGKTTTTAKLAAR VM+HGPGKL LITTD Y Sbjct: 204 ILEKNLQAIPNEDEILNRGGVFALIGPTGVGKTTTTAKLAARFVMKHGPGKLGLITTDAY 263 Query: 300 RIGGYEQLRIYGKILGVMVHSVKDESDLRIALDELRGKHTVLIDTAGMGQRDQMVAEQAA 359 RIGG+EQLRIYGKILGVMVH+VKDE+DL+IAL+EL+GKHT+LIDT G+ Q+D+MVAEQ A Sbjct: 264 RIGGHEQLRIYGKILGVMVHAVKDEADLKIALEELKGKHTILIDTVGVSQKDRMVAEQIA 323 Query: 360 MLSGAGTDIKRLLCLNATATGGTLNEVVSAYSGDGLAGCIITKLDEAATIGNVLDVVIRQ 419 MLS IK+LLCLNAT TG TL +VV AY L GCIITKLDEAATIG+ LDV+IR+ Sbjct: 324 MLSSTRQPIKKLLCLNATNTGETLTDVVRAYKVKQLDGCIITKLDEAATIGSALDVIIRE 383 Query: 420 KLNLYYVANGQRVPEDLHVANRLYLADRAFKL-KRETAPFEFQDAELPVVVGPTA 473 KL LYYVANGQRVPEDLHVAN+ L RAF+ + T+ F+F+ ELP ++G T+ Sbjct: 384 KLRLYYVANGQRVPEDLHVANKKLLIHRAFRAGAQNTSSFQFKHNELPFLMGNTS 438