BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0035.p (319 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IPX0.1 D8IPX0_HERSS D-isomer specific 2-hydroxyacid dehydrogen... 505 e-141 B9Z7V4.1 B9Z7V4_9NEIS D-isomer specific 2-hydroxyacid dehydrogen... 381 e-104 Q5TM04.1 Q5TM04_PSEF5 2-ketogluconate 6-phosphate reductase, put... 378 e-103 C4LFM3.1 C4LFM3_TOLAT D-isomer specific 2-hydroxyacid dehydrogen... 376 e-102 D4BY25.1 D4BY25_PRORE Glyoxylate/hydroxypyruvate reductase B 375 e-102 >D8IPX0.1 D8IPX0_HERSS D-isomer specific 2-hydroxyacid dehydrogenase protein EC=1.1.1.- Length = 319 Score = 505 bits (1300), Expect = e-141, Method: Compositional matrix adjust. Identities = 251/319 (78%), Positives = 278/319 (87%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAALATAQGMLGASLPIGNDM 60 MK HVVVYKKLP+ L+QRL+ EF++T F+ + NRA F+AALATA GM+GASLPI N M Sbjct: 1 MKPHVVVYKKLPEHLVQRLQAEFDLTLFDRITDDNRADFIAALATAHGMIGASLPITNAM 60 Query: 61 LEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVVE 120 LEAAP LKIVST+SVGVDNFDL+YFR+RGLMLAHTPGVL EATAD IF+LILASARRVVE Sbjct: 61 LEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVVE 120 Query: 121 LAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRPN 180 LAE+VKAG+WKGSIGE+ FGVNVHGKTLG++GMGRIGSAVARRAHHGFGM I+YHN RP+ Sbjct: 121 LAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPD 180 Query: 181 PEAEALLAATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRGRI 240 PEAE L A YVS+++L QADFVC MLPLT QTER GA EF LMK AIFINASRGRI Sbjct: 181 PEAERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRI 240 Query: 241 VDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMAEL 300 VDE+ALI ALQ KTI+GAGLDVFE EPLP+ SPLL+LPNVVALPHIGSATHETR AMAEL Sbjct: 241 VDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRLAMAEL 300 Query: 301 AVDNLIAGLRGAQPAHLAV 319 AV NLIAGLRG AHLAV Sbjct: 301 AVTNLIAGLRGEPVAHLAV 319 >B9Z7V4.1 B9Z7V4_9NEIS D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding Length = 335 Score = 381 bits (979), Expect = e-104, Method: Compositional matrix adjust. Identities = 196/314 (62%), Positives = 243/314 (77%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAALATAQGMLGASLPIGNDM 60 MK+ VV YKKLP LL RL ++FEV F+ VN NRA F A+A A G++G+S+ I + Sbjct: 4 MKKRVVAYKKLPPALLARLAEQFEVIGFDGVNDTNRAAFAEAVAGAHGLIGSSVRIDATL 63 Query: 61 LEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVVE 120 L+ A L+++ST+SVG D FD++Y +RG++LA+TP VL E TAD +F+LILA+ARRVVE Sbjct: 64 LDPARELELISTISVGYDTFDVDYLTRRGILLANTPDVLTETTADTLFALILATARRVVE 123 Query: 121 LAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRPN 180 LAEFVKAG W+ SIG + FGV+VH KTLG++G+GRIG AVARRA+ GFGM +VY++ Sbjct: 124 LAEFVKAGHWQRSIGPAHFGVDVHHKTLGLIGLGRIGQAVARRANLGFGMNVVYYSRHAV 183 Query: 181 PEAEALLAATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRGRI 240 PEAEA A V +EL+ ADFVC +LPLTA+T++ I A F M+P IFIN SRG I Sbjct: 184 PEAEAAYGARRVGLNELYASADFVCVVLPLTAETDKLIDADAFARMRPETIFINGSRGPI 243 Query: 241 VDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMAEL 300 VDE+ALI AL+ I+GAGLDVFE+EPLP DSPLLQLPNVVALPHIGSATHETR AMAE+ Sbjct: 244 VDEAALIQALKSGAIHGAGLDVFEREPLPADSPLLQLPNVVALPHIGSATHETRFAMAEM 303 Query: 301 AVDNLIAGLRGAQP 314 AVDNLIAGL G +P Sbjct: 304 AVDNLIAGLNGQRP 317 >Q5TM04.1 Q5TM04_PSEF5 2-ketogluconate 6-phosphate reductase, putative Length = 328 Score = 378 bits (971), Expect = e-103, Method: Compositional matrix adjust. Identities = 183/317 (57%), Positives = 244/317 (76%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAALATAQGMLGASLPIGNDM 60 MK+H+V+YKKL L+ RL+++ +VT E ++A AL +A G+LGASL + ++ Sbjct: 1 MKKHLVLYKKLSPTLMARLQEQAQVTLIETLDAAGLNALRQALPSADGLLGASLRLDAEL 60 Query: 61 LEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVVE 120 L+ AP L+ V++VSVGVDN+D++Y +R ++L++TP VL E TAD F+LILA+ARRVVE Sbjct: 61 LDLAPRLRAVASVSVGVDNYDIDYLTQRRILLSNTPDVLTETTADTGFALILATARRVVE 120 Query: 121 LAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRPN 180 LA V+AG+W+ +IG + FG +VHGKTLG++GMGRIG A+A+R H GFGM ++YH+TRP Sbjct: 121 LANLVRAGQWQRNIGPAHFGTDVHGKTLGIIGMGRIGEALAQRGHFGFGMPLIYHSTRPK 180 Query: 181 PEAEALLAATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRGRI 240 P E A Y S ++L ++ADF+C LPLT +T+ IGAREF LM+P +IFIN SRG++ Sbjct: 181 PAVEQRFNAQYRSLEQLLEEADFICLTLPLTERTQGLIGAREFALMRPESIFINISRGKV 240 Query: 241 VDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMAEL 300 VDE+ALI+ALQ++ I GAGLDVFE+EPL DSPLLQLPNVVA PHIGSATHETR AMA Sbjct: 241 VDEAALIEALQQRRIRGAGLDVFEREPLDHDSPLLQLPNVVATPHIGSATHETREAMARC 300 Query: 301 AVDNLIAGLRGAQPAHL 317 AVDNL+A L G +P +L Sbjct: 301 AVDNLLAALAGQRPPNL 317 >C4LFM3.1 C4LFM3_TOLAT D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding Length = 324 Score = 376 bits (966), Expect = e-102, Method: Compositional matrix adjust. Identities = 181/316 (57%), Positives = 231/316 (73%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAALATAQGMLGASLPIGNDM 60 MK +VV+Y+K+PDDLL +L+++ +VTYF+ +NA NRA F+ ALA A+G++G L +++ Sbjct: 1 MKPNVVLYRKIPDDLLAKLQEQCQVTYFDGINADNRAAFIKALAEAEGVIGVGLQFSDEL 60 Query: 61 LEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVVE 120 + AAP LK VST+SVG D+F++ RG+ L HTPGVL E TAD IF+L+L +ARR+ E Sbjct: 61 MAAAPKLKAVSTISVGYDDFNVAKLSARGIALMHTPGVLTETTADTIFTLVLCAARRITE 120 Query: 121 LAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRPN 180 LAE VK G+W+GSIG +G NVHGKT+G+LGMGRIG AVA+RAH GFGM ++Y+N Sbjct: 121 LAEKVKQGEWQGSIGPDWYGSNVHGKTIGILGMGRIGYAVAKRAHFGFGMNVIYYNRSAK 180 Query: 181 PEAEALLAATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRGRI 240 PEAE L A S DEL ADFVC +LPL +T I MKP A FIN RG Sbjct: 181 PEAEQQLNARRCSLDELLADADFVCNVLPLMPETRHIINRDTLAKMKPSAFFINGGRGAS 240 Query: 241 VDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMAEL 300 VDE+AL++AL+ I GAGLDVFE EPLPV+S LL LPNVVALPHIGSATHETR M+++ Sbjct: 241 VDEAALVEALKSGVIKGAGLDVFETEPLPVNSELLSLPNVVALPHIGSATHETRYEMSKM 300 Query: 301 AVDNLIAGLRGAQPAH 316 AV+NL+A L+G H Sbjct: 301 AVENLLAALKGDLTKH 316 >D4BY25.1 D4BY25_PRORE Glyoxylate/hydroxypyruvate reductase B Length = 330 Score = 375 bits (963), Expect = e-102, Method: Compositional matrix adjust. Identities = 182/311 (58%), Positives = 226/311 (72%) Query: 1 MKQHVVVYKKLPDDLLQRLRDEFEVTYFEAVNAGNRAQFLAALATAQGMLGASLPIGNDM 60 MKQ++++YK +P D L+RL+ F VT F++V N A F AL+ A G++GAS PI D Sbjct: 7 MKQNIILYKSIPTDQLERLQQHFNVTVFDSVTPENLASFKQALSRADGIIGASYPITADD 66 Query: 61 LEAAPLLKIVSTVSVGVDNFDLEYFRKRGLMLAHTPGVLNEATADAIFSLILASARRVVE 120 + AP LK ST+SVG+D FD++ R + L HTP VL E TAD IF+L+L SARR++E Sbjct: 67 IANAPNLKAASTISVGIDQFDIDAMNARKIALMHTPNVLTETTADTIFTLVLCSARRIIE 126 Query: 121 LAEFVKAGKWKGSIGESLFGVNVHGKTLGMLGMGRIGSAVARRAHHGFGMKIVYHNTRPN 180 +AE VK G+W SIGE +G NV+GKT+G+LGMGRIG AVA+RA+ GFGM ++Y+N + Sbjct: 127 MAEMVKNGQWTQSIGEDAYGSNVNGKTIGILGMGRIGYAVAKRAYLGFGMPVLYYNNHIH 186 Query: 181 PEAEALLAATYVSRDELFQQADFVCPMLPLTAQTERTIGAREFGLMKPGAIFINASRGRI 240 P+AE L A D L ++DFVC +LPL+A TE+ IG E MKP A IN SRGRI Sbjct: 187 PDAETQLKARRCDLDTLLAESDFVCVVLPLSASTEKLIGKNELAKMKPSAFLINGSRGRI 246 Query: 241 VDESALIDALQRKTIYGAGLDVFEQEPLPVDSPLLQLPNVVALPHIGSATHETRCAMAEL 300 VDE+ALIDAL+ TI GAGLDVFE EPLP +S LL LPNVVALPHIGSATHETR AM E Sbjct: 247 VDEAALIDALESGTIRGAGLDVFEVEPLPSNSKLLTLPNVVALPHIGSATHETRYAMVEC 306 Query: 301 AVDNLIAGLRG 311 AVDNLIA L+G Sbjct: 307 AVDNLIAALKG 317