BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0116.p (380 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value E6V3A0.1 E6V3A0_VARPD Gentisate 1,2-dioxygenase 479 e-133 A8V9C8.1 A8V9C8_9BURK Gentisate 1,2-dioxygenase 476 e-132 D4X3R9.1 D4X3R9_9BURK Gentisate 1,2-dioxygenase EC=1.13.11.4 475 e-132 E3HJF5.1 E3HJF5_ACHXA Gentisate 1,2-dioxygenase 2 EC=1.13.11.4 472 e-131 E5U8B8.1 E5U8B8_ALCXX Gentisate 1,2-dioxygenase 471 e-131 >E6V3A0.1 E6V3A0_VARPD Gentisate 1,2-dioxygenase Length = 365 Score = 479 bits (1234), Expect = e-133, Method: Compositional matrix adjust. Identities = 234/344 (68%), Positives = 267/344 (77%), Gaps = 1/344 (0%) Query: 30 QTAERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGEVITAE 89 AER Y +++ LNL PLWESLH LVP++P VPALWRY +I LM + E+ITAE Sbjct: 19 NAAERSDYYARIRPLNLTPLWESLHALVPREPQTPCVPALWRYDEIRPLLMESAELITAE 78 Query: 90 EAVRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQGAYTTV 149 EAVRRVLVLENP LPGNSSITQS+YAGLQLI+P EVAP+HRH QSALRFIVDG+GAYTTV Sbjct: 79 EAVRRVLVLENPALPGNSSITQSIYAGLQLIMPGEVAPSHRHVQSALRFIVDGKGAYTTV 138 Query: 150 NGERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAENNSSA 209 GER+TM+PGDFIITPSW WHDHGN GI GVSEPVVWLDGLDIPM+RFFDAGFAEN+ + Sbjct: 139 GGERTTMYPGDFIITPSWAWHDHGNEGIEGVSEPVVWLDGLDIPMLRFFDAGFAENDDAK 198 Query: 210 SQIPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLDEWHGI 269 Q + EG S A +G+NM PVR H SPI NYPYARSREAL L + D W G Sbjct: 199 VQHVARPEGHSLARFGHNMVPVRHDHVSATSPIFNYPYARSREALAQLQKQEAPDAWLGH 258 Query: 270 KLRYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQSFSFS 329 KLRY+NP+TGG PMPTI+ LQLLPKGF GK HR++DG VY VVEGHG +IA Q F F Sbjct: 259 KLRYINPLTGGSPMPTISTNLQLLPKGFAGKTHRATDGAVYSVVEGHGIAEIAGQRFEFG 318 Query: 330 PKDTFVVPSWAPVCFDTPA-DTVLFSFSDRPVLEATGLLREEYF 372 P+DTFVVPSWAP+ +P D VLFSFSDRPV +A G+LRE + Sbjct: 319 PRDTFVVPSWAPLRLLSPVDDVVLFSFSDRPVQQAMGILREAFL 362 >A8V9C8.1 A8V9C8_9BURK Gentisate 1,2-dioxygenase Length = 353 Score = 476 bits (1224), Expect = e-132, Method: Compositional matrix adjust. Identities = 230/342 (67%), Positives = 268/342 (78%), Gaps = 2/342 (0%) Query: 33 ERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGEVITAEEAV 92 +R+ + +LNL+PLWESLH LVPKQP VPALW+Y ++ LMR+GE+ITAEEAV Sbjct: 10 DRRHFNEDIAKLNLSPLWESLHNLVPKQPKSPCVPALWKYREVKPSLMRSGELITAEEAV 69 Query: 93 RRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQGAYTTVNGE 152 RRVLV ENPGL G SSIT +LYAGLQLILP E+AP+HRH QSALRFIVDG+GAYTTVNGE Sbjct: 70 RRVLVFENPGLLGQSSITHTLYAGLQLILPGEIAPSHRHVQSALRFIVDGKGAYTTVNGE 129 Query: 153 RSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAENNSSASQI 212 R+TM PGDFIITPSWTWHDHGN GI G +EPV+WLDGLDIP++RFFDAGFAEN++ ASQ Sbjct: 130 RTTMFPGDFIITPSWTWHDHGNEGIEGQAEPVIWLDGLDIPILRFFDAGFAENDTEASQR 189 Query: 213 PTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLDEWHGIKLR 272 ++ EG S A +GYNMAPV+ + SPI NYPY RSREAL TL ++ +D W G KLR Sbjct: 190 VSRPEGTSAAEFGYNMAPVKNVGAMTTSPIFNYPYERSREALGTLAKTQAIDVWQGYKLR 249 Query: 273 YVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQSFSFSPKD 332 Y+NP+TGG PMPT+A FLQLLP GFQGK HRS+DGTVY VVEG G I D F F PKD Sbjct: 250 YINPLTGGAPMPTMAPFLQLLPAGFQGKSHRSTDGTVYSVVEGSGTAYIGDSVFRFEPKD 309 Query: 333 TFVVPSWAPVCF--DTPADTVLFSFSDRPVLEATGLLREEYF 372 TFVVPSW + D AD VLFSFSDRP+ +A +LREE+ Sbjct: 310 TFVVPSWNSIRINADAGADVVLFSFSDRPMQQAASMLREEFL 351 >D4X3R9.1 D4X3R9_9BURK Gentisate 1,2-dioxygenase EC=1.13.11.4 Length = 354 Score = 475 bits (1222), Expect = e-132, Method: Compositional matrix adjust. Identities = 227/351 (64%), Positives = 272/351 (77%), Gaps = 1/351 (0%) Query: 20 PNSNTPMKIDQTAERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHL 79 P+ +TP D++A+R A Y ++ + ++APLWESLH LVP+QP PR +PALW+Y D+ + Sbjct: 2 PDGHTP-GTDRSADRSAYYARIAKKHMAPLWESLHNLVPRQPTPRCIPALWKYDDVREDV 60 Query: 80 MRAGEVITAEEAVRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFI 139 M +G +ITAEEAVRRVL+LENPGLPG +SITQSLYAGLQLILP E+AP+HRH+QSALRFI Sbjct: 61 MASGSLITAEEAVRRVLILENPGLPGQASITQSLYAGLQLILPGEIAPSHRHTQSALRFI 120 Query: 140 VDGQGAYTTVNGERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFD 199 V+G+GAYT VNGER+TMHPGDFIITPSWTWHDHGN EPVVWLDGLDIP++RF D Sbjct: 121 VEGRGAYTAVNGERTTMHPGDFIITPSWTWHDHGNADTVEGGEPVVWLDGLDIPLLRFLD 180 Query: 200 AGFAENNSSASQIPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFR 259 AGFAEN A+Q T+ EG S A +G+NMAPVR T SPI +YPY RSREALD L+R Sbjct: 181 AGFAENYPQAAQPVTRPEGDSMARFGHNMAPVRHRATSGTSPIFSYPYERSREALDELYR 240 Query: 260 SAVLDEWHGIKLRYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRV 319 LD W G+KLRYVNP TGGYPMPT+A F+QLLP GFQGK +RS+D TVY VV+G G Sbjct: 241 HGELDPWDGVKLRYVNPATGGYPMPTMATFMQLLPAGFQGKPYRSTDSTVYSVVQGRGIA 300 Query: 320 QIADQSFSFSPKDTFVVPSWAPVCFDTPADTVLFSFSDRPVLEATGLLREE 370 +I D+ F F P+D FV PSW PV D LFS+SDRPV +A G+ REE Sbjct: 301 RIGDEEFHFGPRDVFVAPSWLPVQLAALDDATLFSYSDRPVQDALGVWREE 351 >E3HJF5.1 E3HJF5_ACHXA Gentisate 1,2-dioxygenase 2 EC=1.13.11.4 Length = 350 Score = 472 bits (1215), Expect = e-131, Method: Compositional matrix adjust. Identities = 227/350 (64%), Positives = 267/350 (76%), Gaps = 5/350 (1%) Query: 20 PNSNTPMKIDQTAERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHL 79 P+ +TP A+R A Y ++ ++APLWESLH LVP++P PR VPA+W+Y D+ + Sbjct: 2 PDGHTP-----GADRAAYYARIARKHMAPLWESLHNLVPREPAPRCVPAIWKYEDVRDDV 56 Query: 80 MRAGEVITAEEAVRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFI 139 M +G +ITAEEAVRRVL+LENPGLPG +SITQSLYAGLQLILP E+AP+HRH+QSALRFI Sbjct: 57 MASGSLITAEEAVRRVLILENPGLPGQASITQSLYAGLQLILPGEIAPSHRHTQSALRFI 116 Query: 140 VDGQGAYTTVNGERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFD 199 V+G+GAYT VNGER+TMHPGDFIITPSWTWHDHGN A EPVVWLDGLDIP++RF D Sbjct: 117 VEGRGAYTAVNGERTTMHPGDFIITPSWTWHDHGNAETAEGGEPVVWLDGLDIPLLRFLD 176 Query: 200 AGFAENNSSASQIPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFR 259 AGFAEN SA+Q T+ EG S A YG+NMAPVR SPI NYPY RSREALD L+R Sbjct: 177 AGFAENYPSATQPVTRPEGDSMARYGHNMAPVRHQTASGTSPIFNYPYERSREALDQLYR 236 Query: 260 SAVLDEWHGIKLRYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRV 319 LD W G+KLRY+NP TGGYPMPT+A F+Q LP GFQGK +RS+D TVY VVEG G Sbjct: 237 HGELDPWDGVKLRYLNPATGGYPMPTMATFMQFLPAGFQGKTYRSTDSTVYSVVEGRGTA 296 Query: 320 QIADQSFSFSPKDTFVVPSWAPVCFDTPADTVLFSFSDRPVLEATGLLRE 369 +I DQ F F P+D FV PSW PV D LFS+SDRPV +A G+ RE Sbjct: 297 RIGDQEFHFGPRDVFVAPSWQPVQLAALDDATLFSYSDRPVQDALGVWRE 346 >E5U8B8.1 E5U8B8_ALCXX Gentisate 1,2-dioxygenase Length = 350 Score = 471 bits (1213), Expect = e-131, Method: Compositional matrix adjust. Identities = 224/342 (65%), Positives = 265/342 (77%) Query: 32 AERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGEVITAEEA 91 A+R A Y ++ + ++APLWESLH LVP+QP PR VPA+W+Y ++ ++ +G +I+AEEA Sbjct: 9 ADRAAYYARIAQKHMAPLWESLHNLVPRQPAPRCVPAIWKYDEVRDDVLASGSLISAEEA 68 Query: 92 VRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQGAYTTVNG 151 VRRVL+LENPGLPG +SITQSLYAGLQLILP E+AP+HRH+QSALRFIV+G+GAYT VNG Sbjct: 69 VRRVLILENPGLPGQASITQSLYAGLQLILPGEIAPSHRHTQSALRFIVEGRGAYTAVNG 128 Query: 152 ERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAENNSSASQ 211 ER+TMHPGDFIITPSWTWHDHGN EPVVWLDGLDIP++RF DAGFAEN SA+Q Sbjct: 129 ERTTMHPGDFIITPSWTWHDHGNAETVDGGEPVVWLDGLDIPLLRFLDAGFAENYPSATQ 188 Query: 212 IPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLDEWHGIKL 271 T+ EG S A YG+NMAPVR T SPI NYPY RSREALD L+R LD W G+KL Sbjct: 189 PVTRPEGDSMARYGHNMAPVRHRATSGTSPIFNYPYERSREALDALYRHGELDPWDGVKL 248 Query: 272 RYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQSFSFSPK 331 RY+NP TGGYPMPT+A F+QLLP GFQGK +RS+D TVY VVEG G ++ D F F P+ Sbjct: 249 RYLNPATGGYPMPTMATFMQLLPAGFQGKVYRSTDSTVYSVVEGRGIARVGDAEFHFGPR 308 Query: 332 DTFVVPSWAPVCFDTPADTVLFSFSDRPVLEATGLLREEYFA 373 D FV PSW PV D LFS+SDRPV +A GL REE A Sbjct: 309 DVFVAPSWQPVQLAALEDATLFSYSDRPVQDALGLWREERTA 350