BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0167.p (318 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IPY0.1 D8IPY0_HERSS Cobalamin biosynthesis transmembrane prote... 516 e-144 F1VV88.1 F1VV88_9BURK Putative cobalamin biosynthesis protein 490 e-136 A6SVI9.1 A6SVI9_JANMA Cobalamin biosynthesis protein CobD EC=2.6... 443 e-122 A4G2U0.1 A4G2U0_HERAR Cobalamin biosynthesis protein CobD 1 430 e-118 C3XB65.1 C3XB65_OXAFO Cobalamin biosynthesis protein CobD 328 7e-88 >D8IPY0.1 D8IPY0_HERSS Cobalamin biosynthesis transmembrane protein EC=2.6.1.- Length = 319 Score = 516 bits (1329), Expect = e-144, Method: Compositional matrix adjust. Identities = 261/319 (81%), Positives = 287/319 (89%), Gaps = 1/319 (0%) Query: 1 MTFLSILFALLIEQLKPLRADNPIYAWIKSFAARIENWFNAGHAHHGRLGWFVMVGALTV 60 MTFLSILFALLIEQLKPLRADNP+YA IKSFAA IE WFNAG HHGRLGW MVGALTV Sbjct: 1 MTFLSILFALLIEQLKPLRADNPVYASIKSFAATIEGWFNAGQVHHGRLGWVAMVGALTV 60 Query: 61 PAGLIYWVCLRISPLAALAWNVLIVYMTLGFRHYSHYFTSIQLALNTGDDVAARALLAEW 120 P L+YW+ L +S +AA AWNVL+VY+TLGFRHYSHYFTSIQLALN+GD++ ARALLAEW Sbjct: 61 PVALVYWLLLHVSLVAAFAWNVLVVYLTLGFRHYSHYFTSIQLALNSGDEMTARALLAEW 120 Query: 121 TKEDTSDLDVSEISRIAAEKALVTTHRHVFGVFFWFLMPVGPALAVMYRVSEYLARAWNE 180 T++DT+ +DVSEISRIA+E+AL+TTHRHVFGVFFWFLMPVGPALAVMYRV+EYL+RAWNE Sbjct: 121 TRQDTTGMDVSEISRIASERALITTHRHVFGVFFWFLMPVGPALAVMYRVAEYLSRAWNE 180 Query: 181 PDHMKQEVFGRFAERAFYWIDWIPARFTAAAFAVVGNFEDAIYAWRNFADRWQDEAVGII 240 PDHMK E FGRFA +AFYWIDWIPAR TAAAFAVVGNFEDAIYAWRNFA RW DE+ GII Sbjct: 181 PDHMKFEAFGRFAAQAFYWIDWIPARLTAAAFAVVGNFEDAIYAWRNFASRWDDESAGII 240 Query: 241 LSAGGGAMGVRFGTPAESASG-VLPIDAATVDSTLEAADGFPGDQPSPRTLQSTVGLVWR 299 L+AGGGAMGVRFGT E+A+G +LP DAATVDS E DG PGD PSPRTLQSTVGLVWR Sbjct: 241 LAAGGGAMGVRFGTEKEAAAGIILPADAATVDSMPEETDGLPGDTPSPRTLQSTVGLVWR 300 Query: 300 AMLLWMFLLLLLSVAVWMG 318 A+LLWMFLLLLLSVAVWMG Sbjct: 301 ALLLWMFLLLLLSVAVWMG 319 >F1VV88.1 F1VV88_9BURK Putative cobalamin biosynthesis protein Length = 318 Score = 490 bits (1262), Expect = e-136, Method: Compositional matrix adjust. Identities = 242/318 (76%), Positives = 277/318 (87%) Query: 1 MTFLSILFALLIEQLKPLRADNPIYAWIKSFAARIENWFNAGHAHHGRLGWFVMVGALTV 60 MTFLSIL ALLIEQLKPLRADNPIY IK AAR+E WFNAGHAHHG+LGWF+++ AL V Sbjct: 1 MTFLSILCALLIEQLKPLRADNPIYGGIKLLAARMELWFNAGHAHHGKLGWFLVMAALMV 60 Query: 61 PAGLIYWVCLRISPLAALAWNVLIVYMTLGFRHYSHYFTSIQLALNTGDDVAARALLAEW 120 P LIYW+ R+SP AALAWN+LIVY+TLGFRHYSHY++SIQLALN+GDD AAR LLAEW Sbjct: 61 PTALIYWLASRVSPFAALAWNILIVYLTLGFRHYSHYYSSIQLALNSGDDAAARILLAEW 120 Query: 121 TKEDTSDLDVSEISRIAAEKALVTTHRHVFGVFFWFLMPVGPALAVMYRVSEYLARAWNE 180 TK+DTS ++ SEI+RIA EKALVTTHR+VFGVFFWFLMPVGPA AVMYRV+EYLARAWNE Sbjct: 121 TKQDTSTMESSEIARIAVEKALVTTHRNVFGVFFWFLMPVGPACAVMYRVAEYLARAWNE 180 Query: 181 PDHMKQEVFGRFAERAFYWIDWIPARFTAAAFAVVGNFEDAIYAWRNFADRWQDEAVGII 240 P+HMK E FG FA RAFYWIDWIPAR TA AFA+VGNFEDAIYAWRNFA RWQDEAVGII Sbjct: 181 PEHMKNEAFGVFAARAFYWIDWIPARLTAIAFAIVGNFEDAIYAWRNFASRWQDEAVGII 240 Query: 241 LSAGGGAMGVRFGTPAESASGVLPIDAATVDSTLEAADGFPGDQPSPRTLQSTVGLVWRA 300 L+AG GA+G+R GTP + A+ V+P+D T+D+T +D PG++P+ R LQSTVGLVWRA Sbjct: 241 LAAGSGALGIRLGTPLQGAANVMPVDPTTLDTTELESDAAPGEEPTARALQSTVGLVWRA 300 Query: 301 MLLWMFLLLLLSVAVWMG 318 +LLWM LLLLLSVAVW+G Sbjct: 301 LLLWMLLLLLLSVAVWLG 318 >A6SVI9.1 A6SVI9_JANMA Cobalamin biosynthesis protein CobD EC=2.6.1.- Length = 318 Score = 443 bits (1139), Expect = e-122, Method: Compositional matrix adjust. Identities = 220/318 (69%), Positives = 259/318 (81%) Query: 1 MTFLSILFALLIEQLKPLRADNPIYAWIKSFAARIENWFNAGHAHHGRLGWFVMVGALTV 60 MTF SI FALLIEQLKPLRADNP+YA ++ AA++E FNAG AHHGRL W +M+ A + Sbjct: 1 MTFFSIFFALLIEQLKPLRADNPVYAGARALAAKLEGSFNAGQAHHGRLAWALMIAAFCI 60 Query: 61 PAGLIYWVCLRISPLAALAWNVLIVYMTLGFRHYSHYFTSIQLALNTGDDVAARALLAEW 120 P L+YW+ +++ PLAAL NV +VY+TLGFRHYSHYFTSIQLALN GD+ AR+LLAEW Sbjct: 61 PVALLYWISVKLGPLAALIVNVGVVYLTLGFRHYSHYFTSIQLALNNGDEATARSLLAEW 120 Query: 121 TKEDTSDLDVSEISRIAAEKALVTTHRHVFGVFFWFLMPVGPALAVMYRVSEYLARAWNE 180 TK DT+ +DVSE++RIA E ALVTTHR+VFGVFFW L P+GP AVMYRV+EYLAR WNE Sbjct: 121 TKRDTAGMDVSEVARIAVETALVTTHRNVFGVFFWILTPLGPVGAVMYRVAEYLARVWNE 180 Query: 181 PDHMKQEVFGRFAERAFYWIDWIPARFTAAAFAVVGNFEDAIYAWRNFADRWQDEAVGII 240 P ++E FGRFA +AFYWIDWIPAR TAAAFAVVGNFEDAIYAWRNFA RWQDEA+GII Sbjct: 181 PKMAEREEFGRFASQAFYWIDWIPARLTAAAFAVVGNFEDAIYAWRNFAGRWQDEAIGII 240 Query: 241 LSAGGGAMGVRFGTPAESASGVLPIDAATVDSTLEAADGFPGDQPSPRTLQSTVGLVWRA 300 LSAGGGAMG+R G P E+ + V+P DAA VDS + G++ S R LQSTVGLVWRA Sbjct: 241 LSAGGGAMGIRLGAPVETVAEVIPADAAMVDSINIETEAPLGEEASSRALQSTVGLVWRA 300 Query: 301 MLLWMFLLLLLSVAVWMG 318 +LLWM L+LLLS+A W+G Sbjct: 301 LLLWMLLVLLLSIAKWLG 318 >A4G2U0.1 A4G2U0_HERAR Cobalamin biosynthesis protein CobD 1 Length = 318 Score = 430 bits (1106), Expect = e-118, Method: Compositional matrix adjust. Identities = 222/318 (69%), Positives = 261/318 (82%) Query: 1 MTFLSILFALLIEQLKPLRADNPIYAWIKSFAARIENWFNAGHAHHGRLGWFVMVGALTV 60 MTF SILFALLIEQLKPLRADNP+YA ++ A ++E FNAG HGR+ WF+M+ A V Sbjct: 1 MTFFSILFALLIEQLKPLRADNPVYAGARALAEKLEKSFNAGQVQHGRIAWFLMIAAFIV 60 Query: 61 PAGLIYWVCLRISPLAALAWNVLIVYMTLGFRHYSHYFTSIQLALNTGDDVAARALLAEW 120 P L+YW+ L+I PLAAL NV +VY+TLGFRHYSH+FTSIQLALN GD+ AR+LLAEW Sbjct: 61 PVALLYWISLKIGPLAALVVNVAVVYVTLGFRHYSHFFTSIQLALNNGDEATARSLLAEW 120 Query: 121 TKEDTSDLDVSEISRIAAEKALVTTHRHVFGVFFWFLMPVGPALAVMYRVSEYLARAWNE 180 TK DT+D+DVSE++RIA EKALVTTHR+VFGVFFW L P+GP A+MYRV+EYLARAWNE Sbjct: 121 TKRDTADMDVSEVARIAVEKALVTTHRNVFGVFFWILTPLGPVGAIMYRVAEYLARAWNE 180 Query: 181 PDHMKQEVFGRFAERAFYWIDWIPARFTAAAFAVVGNFEDAIYAWRNFADRWQDEAVGII 240 P ++E FGRFA +AFYWIDWIP R TAAAFAVVGNFEDAIYAWRNFA RWQDEAVGII Sbjct: 181 PKAAEREEFGRFAAQAFYWIDWIPVRLTAAAFAVVGNFEDAIYAWRNFAGRWQDEAVGII 240 Query: 241 LSAGGGAMGVRFGTPAESASGVLPIDAATVDSTLEAADGFPGDQPSPRTLQSTVGLVWRA 300 LSAGGGAMG+R G E+A+ V+P+DA TVDS + G++ S R LQSTVGLVWRA Sbjct: 241 LSAGGGAMGIRLGASVENAADVIPVDAGTVDSITLETEAPVGEEASVRALQSTVGLVWRA 300 Query: 301 MLLWMFLLLLLSVAVWMG 318 +LLWM L+LLLS+A W+G Sbjct: 301 LLLWMLLVLLLSIAKWLG 318 >C3XB65.1 C3XB65_OXAFO Cobalamin biosynthesis protein CobD Length = 316 Score = 328 bits (841), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 146/300 (48%), Positives = 210/300 (70%), Gaps = 2/300 (0%) Query: 1 MTFLSILFALLIEQLKPLRADNPIYAWIKSFAARIENWFNAGHAHHGRLGWFVMVGALTV 60 M F SIL ALLI+ +P+R +N A +++ A + +W + G +HHG LGW V L + Sbjct: 1 MFFFSILCALLIDHFRPMRTNNLFLALLRATGAHVRSWCDTGTSHHGCLGWIVANAMLAI 60 Query: 61 PAGLIYWVCLRISPLAALAWNVLIVYMTLGFRHYSHYFTSIQLALNTGDDVAARALLAEW 120 P ++YW+C I PL L W++LI+Y+ +GFR+Y+HYF++IQ+AL G+ + AR LL EW Sbjct: 61 PVLMVYWICQLIHPLLGLLWSILILYLCMGFRNYNHYFSAIQMALLNGEQMQARNLLGEW 120 Query: 121 TKEDTSDLDVSEISRIAAEKALVTTHRHVFGVFFWFLMPVGPALAVMYRVSEYLARAWNE 180 D S +D ++I+R+A EKAL++ + VFG+ FWF++P GPA A++YR S YL ++WNE Sbjct: 121 CDCDASAMDTADITRMAIEKALISVQKDVFGIIFWFVLPFGPAGAILYRFSSYLQKSWNE 180 Query: 181 PDHMKQEVFGRFAERAFYWIDWIPARFTAAAFAVVGNFEDAIYAWRNFADRWQDEAVGII 240 PD + FG FA++ FYWIDW+P R TA +FA+VGNFEDAIYAWRNFA RW++ +GI+ Sbjct: 181 PD--DSDSFGLFAKKIFYWIDWVPVRLTALSFAIVGNFEDAIYAWRNFAGRWKNRNIGIL 238 Query: 241 LSAGGGAMGVRFGTPAESASGVLPIDAATVDSTLEAADGFPGDQPSPRTLQSTVGLVWRA 300 L++ GGAMGV+ GTP E A +D + + ++ FPG++P R LQS +GL+WRA Sbjct: 239 LASAGGAMGVKLGTPFERAIRGTFVDLNSFVTMGYESESFPGEEPKIRYLQSALGLIWRA 298