BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0164.p (462 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IPY2.1 D8IPY2_HERSS Fumarate hydratase class II (Fumarase) pro... 827 0.0 Q5QCQ9.1 Q5QCQ9_HERAR Putative furamase EC=4.2.1.2 731 0.0 A6T1A6.1 A6T1A6_JANMA Fumarate hydratase, class II EC=4.2.1.2 724 0.0 E3HHM0.1 E3HHM0_ACHXA Fumarate hydratase, class II EC=4.2.1.2 719 0.0 E5UDT2.1 E5UDT2_ALCXX Fumarate hydratase class II 719 0.0 >D8IPY2.1 D8IPY2_HERSS Fumarate hydratase class II (Fumarase) protein EC=4.2.1.2 Length = 462 Score = 827 bits (2135), Expect = 0.0, Method: Compositional matrix adjust. Identities = 407/461 (88%), Positives = 432/461 (93%) Query: 1 MKHRIERDTFGPIEVPDDRLWGAQTQRSLHHFHISTERMPAELIVALAAVKRACASVNRD 60 M HR+ERDTFG IEVP DRLWGAQT+RSLHHFHISTERMPAELIVALAAVKRACASVNRD Sbjct: 1 MNHRMERDTFGLIEVPADRLWGAQTERSLHHFHISTERMPAELIVALAAVKRACASVNRD 60 Query: 61 LGKLDAKKAEGIVAAADEVIAGKHPLEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 LGKLD KKAE I+AAADEVIAGKHPLEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV Sbjct: 61 LGKLDGKKAEAIIAAADEVIAGKHPLEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 Query: 121 RGEERKIHPNDDVNQSQSSNDIFPTAMHVAASLAVATHLLPKLHKLRATLERKSEEFRDI 180 RGE+R IHPNDDVN+ QSSNDIFPTAMHVAA +AVATHL+P LH LR TL KS +F DI Sbjct: 121 RGEDRLIHPNDDVNRGQSSNDIFPTAMHVAACMAVATHLIPALHTLRGTLAEKSSQFADI 180 Query: 181 VKIGRTHLQDATPLTLGQEFSGYIAQLTHAEMAIIATLNAVSELAAGGTAVGTGLNAHPE 240 VKIGRTHLQDATPLTLGQEFSGY+AQL HAE A+I TLNA+SELAAGGTAVGTGLNAHPE Sbjct: 181 VKIGRTHLQDATPLTLGQEFSGYVAQLEHAESAVIGTLNAISELAAGGTAVGTGLNAHPE 240 Query: 241 FGTRVAAELEKHYGFPFKTAPNKFAALASHDALVSAHGALKTLAAALMKIANDVRWLASG 300 FG RVAAEL K +GFPFKTAPNKFAALA HDALV++HG LKTLAAALMKIANDVRWLASG Sbjct: 241 FGERVAAELAKFFGFPFKTAPNKFAALAGHDALVASHGGLKTLAAALMKIANDVRWLASG 300 Query: 301 PRSGLGEITIPENEPGSSIMPGKVNPTQCEALTMLCAQVFGNDVAINIGGASGNFELNVF 360 PRSGLGEITIPENEPGSSIMPGKVNPTQCEA+TMLCAQVFGNDVA+NIGGASGNFELNVF Sbjct: 301 PRSGLGEITIPENEPGSSIMPGKVNPTQCEAMTMLCAQVFGNDVALNIGGASGNFELNVF 360 Query: 361 KPVIVHNFLQSVRLLADGMDSFDDHCAQGIEANRDRIADLMERSLMLVTALAPHIGYDKA 420 KP+I+HNFLQSVRLLADGM SF++HCA+GIEAN RI +LME+SLMLVTALAPHIGYDKA Sbjct: 361 KPLIIHNFLQSVRLLADGMASFEEHCARGIEANHSRIGELMEKSLMLVTALAPHIGYDKA 420 Query: 421 AKIAKQAHHDGTTLKQAALALGYVTEEQFALWIKPEEMTGP 461 AKIAKQAHHDGTTLKQAAL+LGYVTEEQFA WIKPE+MT P Sbjct: 421 AKIAKQAHHDGTTLKQAALSLGYVTEEQFAEWIKPEQMTRP 461 >Q5QCQ9.1 Q5QCQ9_HERAR Putative furamase EC=4.2.1.2 Length = 462 Score = 731 bits (1888), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/461 (77%), Positives = 403/461 (87%) Query: 1 MKHRIERDTFGPIEVPDDRLWGAQTQRSLHHFHISTERMPAELIVALAAVKRACASVNRD 60 MK RIERDTFGPI V D+RLWGAQTQRSL HF ISTERMP ELIVALA VKRA A N Sbjct: 1 MKTRIERDTFGPIAVNDERLWGAQTQRSLEHFKISTERMPQELIVALAEVKRAAALANLG 60 Query: 61 LGKLDAKKAEGIVAAADEVIAGKHPLEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 LGKLD KKA+ IV+AA+EVIAG+HP EFPL++WQTGSGTQ+NMNMNEVLANRASELLGG Sbjct: 61 LGKLDRKKADAIVSAAEEVIAGQHPHEFPLALWQTGSGTQTNMNMNEVLANRASELLGGE 120 Query: 121 RGEERKIHPNDDVNQSQSSNDIFPTAMHVAASLAVATHLLPKLHKLRATLERKSEEFRDI 180 RGE+R +HPNDDVN QSSNDIFPTAM+VAAS+++ LLP +H LR TL+RK+EEF DI Sbjct: 121 RGEQRLVHPNDDVNLGQSSNDIFPTAMYVAASVSITQKLLPAIHALRVTLQRKAEEFADI 180 Query: 181 VKIGRTHLQDATPLTLGQEFSGYIAQLTHAEMAIIATLNAVSELAAGGTAVGTGLNAHPE 240 VKIGRTHLQDATPLTLGQEFSGY+AQL HAE+ I+ L + ELA GGTAVGTGLN HPE Sbjct: 181 VKIGRTHLQDATPLTLGQEFSGYVAQLHHAELGIVVLLPPLHELAVGGTAVGTGLNTHPE 240 Query: 241 FGTRVAAELEKHYGFPFKTAPNKFAALASHDALVSAHGALKTLAAALMKIANDVRWLASG 300 FGTRVAAEL + G FK+A NKFAALA+HDALVS HG LKTLAA++MKIANDVRWLASG Sbjct: 241 FGTRVAAELTQRSGVHFKSAANKFAALAAHDALVSVHGGLKTLAASMMKIANDVRWLASG 300 Query: 301 PRSGLGEITIPENEPGSSIMPGKVNPTQCEALTMLCAQVFGNDVAINIGGASGNFELNVF 360 PRSGLGE++IPENEPGSSIMPGKVNPTQ EA+TMLC QVFGNDVA+N GGASGNFELNVF Sbjct: 301 PRSGLGELSIPENEPGSSIMPGKVNPTQSEAVTMLCCQVFGNDVALNFGGASGNFELNVF 360 Query: 361 KPVIVHNFLQSVRLLADGMDSFDDHCAQGIEANRDRIADLMERSLMLVTALAPHIGYDKA 420 KP++ HNFLQSVRLL+DG+ FD+HCAQGI ANR+RIADLM RSLMLVTAL PHIGYDKA Sbjct: 361 KPLMAHNFLQSVRLLSDGIHGFDEHCAQGIAANRERIADLMARSLMLVTALTPHIGYDKA 420 Query: 421 AKIAKQAHHDGTTLKQAALALGYVTEEQFALWIKPEEMTGP 461 A+IAKQAHHDGTTLK+ ALALG+V+ E+F W++P++M P Sbjct: 421 AQIAKQAHHDGTTLKETALALGFVSSEEFDRWVQPQDMLHP 461 >A6T1A6.1 A6T1A6_JANMA Fumarate hydratase, class II EC=4.2.1.2 Length = 462 Score = 724 bits (1869), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/461 (77%), Positives = 393/461 (85%) Query: 1 MKHRIERDTFGPIEVPDDRLWGAQTQRSLHHFHISTERMPAELIVALAAVKRACASVNRD 60 M RIERDTFGPIEV RLWGAQTQRSL HF ISTE+MP ELI+ALA VKRA A VN D Sbjct: 1 MTTRIERDTFGPIEVDSQRLWGAQTQRSLEHFKISTEKMPQELIIALAEVKRAAAIVNLD 60 Query: 61 LGKLDAKKAEGIVAAADEVIAGKHPLEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 LGKL KA+ I AA+EVI G+HPLEFPL+VWQTGSGTQSNMNMNEVLANRASELLGG Sbjct: 61 LGKLAKDKAQAIAQAAEEVIDGRHPLEFPLAVWQTGSGTQSNMNMNEVLANRASELLGGE 120 Query: 121 RGEERKIHPNDDVNQSQSSNDIFPTAMHVAASLAVATHLLPKLHKLRATLERKSEEFRDI 180 RGE+R +HPNDDVN QSSNDIFPTAM+VAAS+AV+ LLP L+ATL K+EEF DI Sbjct: 121 RGEKRLVHPNDDVNLGQSSNDIFPTAMYVAASVAVSKTLLPAATALQATLNGKAEEFADI 180 Query: 181 VKIGRTHLQDATPLTLGQEFSGYIAQLTHAEMAIIATLNAVSELAAGGTAVGTGLNAHPE 240 VKIGRTHLQDATPLTLGQEFSGY+AQL + AI A L + +LA GGTAVGTGLN HPE Sbjct: 181 VKIGRTHLQDATPLTLGQEFSGYVAQLAQSTAAIHALLPPLQDLAVGGTAVGTGLNTHPE 240 Query: 241 FGTRVAAELEKHYGFPFKTAPNKFAALASHDALVSAHGALKTLAAALMKIANDVRWLASG 300 FG RVAAEL + G FK+A NKFAALA+HDA+VS HG LKTLAA++MKIANDVRWLASG Sbjct: 241 FGVRVAAELTQRTGVTFKSADNKFAALAAHDAMVSVHGGLKTLAASMMKIANDVRWLASG 300 Query: 301 PRSGLGEITIPENEPGSSIMPGKVNPTQCEALTMLCAQVFGNDVAINIGGASGNFELNVF 360 PRSGLGEI+IPENEPGSSIMPGKVNPTQ EA+TMLC QVFGNDVA+N GGASGNFELNVF Sbjct: 301 PRSGLGEISIPENEPGSSIMPGKVNPTQSEAVTMLCCQVFGNDVALNFGGASGNFELNVF 360 Query: 361 KPVIVHNFLQSVRLLADGMDSFDDHCAQGIEANRDRIADLMERSLMLVTALAPHIGYDKA 420 KP++ HNFLQSVRLLADG+ SFD+HCA GI ANRDRIADLMERSLMLVTALAPHIGYDKA Sbjct: 361 KPLLAHNFLQSVRLLADGIHSFDEHCAHGIVANRDRIADLMERSLMLVTALAPHIGYDKA 420 Query: 421 AKIAKQAHHDGTTLKQAALALGYVTEEQFALWIKPEEMTGP 461 AKIAKQAH G LK+ ALALGYVT E+F LW+KP++M P Sbjct: 421 AKIAKQAHQQGANLKETALALGYVTSEEFDLWVKPQDMIHP 461 >E3HHM0.1 E3HHM0_ACHXA Fumarate hydratase, class II EC=4.2.1.2 Length = 465 Score = 719 bits (1856), Expect = 0.0, Method: Compositional matrix adjust. Identities = 351/459 (76%), Positives = 392/459 (85%) Query: 1 MKHRIERDTFGPIEVPDDRLWGAQTQRSLHHFHISTERMPAELIVALAAVKRACASVNRD 60 MK RIE+DTFGPIEVP+D LWGAQTQRSLH F ISTE+MP L+ A+A +KRA A VN D Sbjct: 1 MKTRIEKDTFGPIEVPEDHLWGAQTQRSLHFFAISTEKMPVPLVEAMARLKRAAAKVNAD 60 Query: 61 LGKLDAKKAEGIVAAADEVIAGKHPLEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 LG+LD K A+GIV AADEVIAGK P EFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV Sbjct: 61 LGELDPKIADGIVRAADEVIAGKWPNEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 Query: 121 RGEERKIHPNDDVNQSQSSNDIFPTAMHVAASLAVATHLLPKLHKLRATLERKSEEFRDI 180 RGEERK+HPND VN+ QSSND FPTAMHVAA++ V HLLP L LRATL KS EF DI Sbjct: 121 RGEERKVHPNDHVNRGQSSNDTFPTAMHVAAAVQVEHHLLPSLKALRATLAAKSAEFYDI 180 Query: 181 VKIGRTHLQDATPLTLGQEFSGYIAQLTHAEMAIIATLNAVSELAAGGTAVGTGLNAHPE 240 VKIGRTHLQDATPLTLGQE SGY+AQL AE I ATL + +LA GGTAVGTGLNAHP+ Sbjct: 181 VKIGRTHLQDATPLTLGQELSGYVAQLDLAEQQIRATLPGLHQLAIGGTAVGTGLNAHPQ 240 Query: 241 FGTRVAAELEKHYGFPFKTAPNKFAALASHDALVSAHGALKTLAAALMKIANDVRWLASG 300 F +V+AEL G F +APNKF LASH+AL+ AHGALK+LAA LMKIANDVRWL+SG Sbjct: 241 FSAKVSAELAHATGTAFVSAPNKFQGLASHEALLFAHGALKSLAAGLMKIANDVRWLSSG 300 Query: 301 PRSGLGEITIPENEPGSSIMPGKVNPTQCEALTMLCAQVFGNDVAINIGGASGNFELNVF 360 PRSGLGEI+IPENEPGSSIMPGKVNPTQCEA+TML AQV GNDVAINIGGASGNFELNVF Sbjct: 301 PRSGLGEISIPENEPGSSIMPGKVNPTQCEAITMLAAQVLGNDVAINIGGASGNFELNVF 360 Query: 361 KPVIVHNFLQSVRLLADGMDSFDDHCAQGIEANRDRIADLMERSLMLVTALAPHIGYDKA 420 KP+++HNFLQSVRLL DGM SF++HCA GIE N +RIA+L++RSLMLVTAL PHIGYDKA Sbjct: 361 KPLVIHNFLQSVRLLTDGMASFNEHCAAGIEPNLERIAELVDRSLMLVTALNPHIGYDKA 420 Query: 421 AKIAKQAHHDGTTLKQAALALGYVTEEQFALWIKPEEMT 459 A+IAK+AH +G +LK++ALALGYVTE QFA W+ P MT Sbjct: 421 AQIAKKAHKEGLSLKESALALGYVTEAQFAEWVVPASMT 459 >E5UDT2.1 E5UDT2_ALCXX Fumarate hydratase class II Length = 463 Score = 719 bits (1855), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/459 (76%), Positives = 392/459 (85%) Query: 1 MKHRIERDTFGPIEVPDDRLWGAQTQRSLHHFHISTERMPAELIVALAAVKRACASVNRD 60 MK RIE+DTFGPIEVP+D LWGAQTQRSLH F ISTE+MP L++A+A +KRA A VN D Sbjct: 1 MKTRIEKDTFGPIEVPEDHLWGAQTQRSLHFFAISTEKMPEPLVIAMARLKRAAAKVNAD 60 Query: 61 LGKLDAKKAEGIVAAADEVIAGKHPLEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 LG+LD K A+GI+ AADEVIAGK P EFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV Sbjct: 61 LGELDPKIADGIIRAADEVIAGKWPNEFPLSVWQTGSGTQSNMNMNEVLANRASELLGGV 120 Query: 121 RGEERKIHPNDDVNQSQSSNDIFPTAMHVAASLAVATHLLPKLHKLRATLERKSEEFRDI 180 RGEERK+HPND VN+ QSSND FPTAMHVAA++ V HLLP L LRAT KS EF DI Sbjct: 121 RGEERKVHPNDHVNRGQSSNDTFPTAMHVAAAVQVEHHLLPALKTLRATFAAKSAEFYDI 180 Query: 181 VKIGRTHLQDATPLTLGQEFSGYIAQLTHAEMAIIATLNAVSELAAGGTAVGTGLNAHPE 240 VKIGRTHLQDATPLTLGQE SGY+AQL AE I ATL + +LA GGTAVGTGLNAHP+ Sbjct: 181 VKIGRTHLQDATPLTLGQELSGYVAQLDLAEQQIRATLPGLHQLAIGGTAVGTGLNAHPQ 240 Query: 241 FGTRVAAELEKHYGFPFKTAPNKFAALASHDALVSAHGALKTLAAALMKIANDVRWLASG 300 F +V+AEL F +APNKF ALASH+AL+ AHGALK+LAA LMKIANDVRWL+SG Sbjct: 241 FSAKVSAELAHATETAFVSAPNKFQALASHEALLFAHGALKSLAAGLMKIANDVRWLSSG 300 Query: 301 PRSGLGEITIPENEPGSSIMPGKVNPTQCEALTMLCAQVFGNDVAINIGGASGNFELNVF 360 PRSGLGEI+IPENEPGSSIMPGKVNPTQCEA+TML AQV GNDVAINIGGASGNFELNV+ Sbjct: 301 PRSGLGEISIPENEPGSSIMPGKVNPTQCEAITMLAAQVMGNDVAINIGGASGNFELNVY 360 Query: 361 KPVIVHNFLQSVRLLADGMDSFDDHCAQGIEANRDRIADLMERSLMLVTALAPHIGYDKA 420 KP+++HNFLQSVRLL DGM SFD HCA GI+ NR+RIA+L++RSLMLVTAL PHIGYDKA Sbjct: 361 KPLVIHNFLQSVRLLTDGMASFDQHCAAGIQPNRERIAELVDRSLMLVTALNPHIGYDKA 420 Query: 421 AKIAKQAHHDGTTLKQAALALGYVTEEQFALWIKPEEMT 459 A+IAK+AH +G +LK++ALALGYVTE QFA W+ P MT Sbjct: 421 AQIAKKAHKEGLSLKESALALGYVTEAQFAEWVVPGSMT 459