BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0084.p (312 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IQ11.1 D8IQ11_HERSS Fumarate hydratase protein EC=4.2.1.2 601 e-170 A2RPM9.1 A2RPM9_HERSE Fumarate hydratase protein 601 e-170 F1W066.1 F1W066_9BURK Fumarate hydratase class I, aerobic 563 e-159 A6SWC3.1 A6SWC3_JANMA Fumarate hydratase, class I EC=4.2.1.2 563 e-159 A4G3L6.1 A4G3L6_HERAR Putative fumarate hydratase class I, aerob... 559 e-157 >D8IQ11.1 D8IQ11_HERSS Fumarate hydratase protein EC=4.2.1.2 Length = 513 Score = 601 bits (1550), Expect = e-170, Method: Compositional matrix adjust. Identities = 292/312 (93%), Positives = 303/312 (97%) Query: 1 MLMAKEVLMDDIDMYELKQRGPQNKTEELRIELCDKVNALGIGAQGLGGLTTVLDVKIAM 60 MLMAKEVLM+DIDMYEL +RGPQNKTEELRIEL +KVNALGIGAQGLGGLTTVLDVKI M Sbjct: 202 MLMAKEVLMEDIDMYELLKRGPQNKTEELRIELYEKVNALGIGAQGLGGLTTVLDVKIMM 261 Query: 61 YPTHAASKPVAMIPNCAATRHGHFVLDGSGPAYMDPPSLSEWPEVHWVPDTEKSKSVNLD 120 +PTHAASKPVAMIPNCAATRHGHFVLDGSGPAYM+PPSLS+WPEVHWV DTEKSK VNLD Sbjct: 262 HPTHAASKPVAMIPNCAATRHGHFVLDGSGPAYMEPPSLSDWPEVHWVADTEKSKRVNLD 321 Query: 121 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQEMLAKGEKLPVDFTNRVIYYVGPVDP 180 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQ+MLAKGEKLPVDFTNRVIYYVGPVDP Sbjct: 322 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDP 381 Query: 181 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGPTAIEAIKKHKSAYLMAVGG 240 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGP AIEAIKKHKSAYLMAVGG Sbjct: 382 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGPAAIEAIKKHKSAYLMAVGG 441 Query: 241 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVDSNGISVHNTGPAEWKEKIAT 300 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVD+NG+SVHNTGPAEWKEKIA Sbjct: 442 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVDANGVSVHNTGPAEWKEKIAQ 501 Query: 301 NTALSKIAVTTA 312 + +LSKI VTTA Sbjct: 502 SVSLSKIPVTTA 513 >A2RPM9.1 A2RPM9_HERSE Fumarate hydratase protein Length = 513 Score = 601 bits (1550), Expect = e-170, Method: Compositional matrix adjust. Identities = 292/312 (93%), Positives = 303/312 (97%) Query: 1 MLMAKEVLMDDIDMYELKQRGPQNKTEELRIELCDKVNALGIGAQGLGGLTTVLDVKIAM 60 MLMAKEVLM+DIDMYEL +RGPQNKTEELRIEL +KVNALGIGAQGLGGLTTVLDVKI M Sbjct: 202 MLMAKEVLMEDIDMYELLKRGPQNKTEELRIELYEKVNALGIGAQGLGGLTTVLDVKIMM 261 Query: 61 YPTHAASKPVAMIPNCAATRHGHFVLDGSGPAYMDPPSLSEWPEVHWVPDTEKSKSVNLD 120 +PTHAASKPVAMIPNCAATRHGHFVLDGSGPAYM+PPSLS+WPEVHWV DTEKSK VNLD Sbjct: 262 HPTHAASKPVAMIPNCAATRHGHFVLDGSGPAYMEPPSLSDWPEVHWVADTEKSKRVNLD 321 Query: 121 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQEMLAKGEKLPVDFTNRVIYYVGPVDP 180 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQ+MLAKGEKLPVDFTNRVIYYVGPVDP Sbjct: 322 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDP 381 Query: 181 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGPTAIEAIKKHKSAYLMAVGG 240 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGP AIEAIKKHKSAYLMAVGG Sbjct: 382 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGPAAIEAIKKHKSAYLMAVGG 441 Query: 241 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVDSNGISVHNTGPAEWKEKIAT 300 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVD+NG+SVHNTGPAEWKEKIA Sbjct: 442 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVDANGVSVHNTGPAEWKEKIAQ 501 Query: 301 NTALSKIAVTTA 312 + +LSKI VTTA Sbjct: 502 SVSLSKIPVTTA 513 >F1W066.1 F1W066_9BURK Fumarate hydratase class I, aerobic Length = 324 Score = 563 bits (1452), Expect = e-159, Method: Compositional matrix adjust. Identities = 270/312 (86%), Positives = 292/312 (93%), Gaps = 1/312 (0%) Query: 1 MLMAKEVLMDDIDMYELKQRGPQNKTEELRIELCDKVNALGIGAQGLGGLTTVLDVKIAM 60 MLMAK+ LM+DIDMYEL+QRGPQNKTEELRIELCDK+NALGIGAQGLGGLTTVLDVKI M Sbjct: 14 MLMAKQSLMEDIDMYELRQRGPQNKTEELRIELCDKINALGIGAQGLGGLTTVLDVKIMM 73 Query: 61 YPTHAASKPVAMIPNCAATRHGHFVLDGSGPAYMDPPSLSEWPEVHWVPDTEKSKSVNLD 120 PTHAASKPVAMIPNCAATRH HFVLDGSG Y++PP LS WP+V WV DTEKSK V+L+ Sbjct: 74 QPTHAASKPVAMIPNCAATRHAHFVLDGSGAVYLEPPLLSSWPDVKWVADTEKSKRVDLN 133 Query: 121 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQEMLAKGEKLPVDFTNRVIYYVGPVDP 180 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQ+MLAKGE LPVDFTNRVIYYVGPVDP Sbjct: 134 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQDMLAKGEALPVDFTNRVIYYVGPVDP 193 Query: 181 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGPTAIEAIKKHKSAYLMAVGG 240 VRDE VGPAGPTTATRMDKFTDMMLEQTGLISM+GK+ERGP AIE+I+KHKSAYLMAVGG Sbjct: 194 VRDEAVGPAGPTTATRMDKFTDMMLEQTGLISMVGKAERGPEAIESIRKHKSAYLMAVGG 253 Query: 241 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVDSNGISVHNTGPAEWKEKIAT 300 +AYLVSKAIKSA V+GFADLGMEAIYEFDV+DMPVTVAVDS+GISVHNTGP EW+EKIA Sbjct: 254 AAYLVSKAIKSATVVGFADLGMEAIYEFDVQDMPVTVAVDSSGISVHNTGPKEWQEKIA- 312 Query: 301 NTALSKIAVTTA 312 ++ALS+I V A Sbjct: 313 HSALSQIPVVAA 324 >A6SWC3.1 A6SWC3_JANMA Fumarate hydratase, class I EC=4.2.1.2 Length = 512 Score = 563 bits (1452), Expect = e-159, Method: Compositional matrix adjust. Identities = 265/298 (88%), Positives = 287/298 (96%) Query: 1 MLMAKEVLMDDIDMYELKQRGPQNKTEELRIELCDKVNALGIGAQGLGGLTTVLDVKIAM 60 MLMAK+VLM+DIDMYELK+RGPQNKTEELRIELCDK+N+LGIGAQGLGGLTTVLDVKI M Sbjct: 202 MLMAKQVLMEDIDMYELKKRGPQNKTEELRIELCDKINSLGIGAQGLGGLTTVLDVKIMM 261 Query: 61 YPTHAASKPVAMIPNCAATRHGHFVLDGSGPAYMDPPSLSEWPEVHWVPDTEKSKSVNLD 120 +PTHAASKPVAMIPNCAATRH HFVLDGSGP Y+DPP+LS+WP+V+W+PDTE+SK V+L+ Sbjct: 262 HPTHAASKPVAMIPNCAATRHAHFVLDGSGPVYLDPPALSDWPKVNWMPDTEQSKRVDLN 321 Query: 121 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQEMLAKGEKLPVDFTNRVIYYVGPVDP 180 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQ+MLAKGEKLPVDFTNR+IYYVGPVDP Sbjct: 322 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRIIYYVGPVDP 381 Query: 181 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGPTAIEAIKKHKSAYLMAVGG 240 VRDEVVGPAGPTTATRMDKFT+MML QTGLISM+GK+ERGPTAIEAIKKHKSAYLMAVGG Sbjct: 382 VRDEVVGPAGPTTATRMDKFTEMMLAQTGLISMVGKAERGPTAIEAIKKHKSAYLMAVGG 441 Query: 241 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVDSNGISVHNTGPAEWKEKI 298 +AYLVSKAIK AKVLGF DLGMEAIYEFDV+DMPVTVAVDS G SVH TGP EWKEKI Sbjct: 442 AAYLVSKAIKKAKVLGFEDLGMEAIYEFDVQDMPVTVAVDSTGTSVHTTGPKEWKEKI 499 >A4G3L6.1 A4G3L6_HERAR Putative fumarate hydratase class I, aerobic (Fumarase) (FumA-like) EC=4.2.1.32 Length = 512 Score = 559 bits (1441), Expect = e-157, Method: Compositional matrix adjust. Identities = 263/298 (88%), Positives = 286/298 (95%) Query: 1 MLMAKEVLMDDIDMYELKQRGPQNKTEELRIELCDKVNALGIGAQGLGGLTTVLDVKIAM 60 MLMAK+VLM+DIDMYELK+RGPQNKTEELRIELCDK+NALGIGAQGLGGLTTVLDVKI M Sbjct: 202 MLMAKQVLMEDIDMYELKKRGPQNKTEELRIELCDKINALGIGAQGLGGLTTVLDVKIMM 261 Query: 61 YPTHAASKPVAMIPNCAATRHGHFVLDGSGPAYMDPPSLSEWPEVHWVPDTEKSKSVNLD 120 +PTHAASKPVAMIPNCAATRH HFVLDGSGP Y+DPPSLS+WP+V+W P+TE SK V+L+ Sbjct: 262 HPTHAASKPVAMIPNCAATRHAHFVLDGSGPVYLDPPSLSDWPDVNWAPNTELSKRVDLN 321 Query: 121 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQEMLAKGEKLPVDFTNRVIYYVGPVDP 180 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQ+MLAKGEKLPVDFTNR+IYYVGPVDP Sbjct: 322 TLTKEEVASWKPGQTLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRIIYYVGPVDP 381 Query: 181 VRDEVVGPAGPTTATRMDKFTDMMLEQTGLISMIGKSERGPTAIEAIKKHKSAYLMAVGG 240 VRDEVVGPAGPTTATRMDKFT+MML QTGLISM+GK+ERGPTAIE+IKKH+SAYLMAVGG Sbjct: 382 VRDEVVGPAGPTTATRMDKFTEMMLAQTGLISMVGKAERGPTAIESIKKHQSAYLMAVGG 441 Query: 241 SAYLVSKAIKSAKVLGFADLGMEAIYEFDVKDMPVTVAVDSNGISVHNTGPAEWKEKI 298 +AYLVSKAIK+AKV+GFADLGMEAIYEFDV DMPVTVAVDS G SVHN GP EWK+KI Sbjct: 442 AAYLVSKAIKTAKVVGFADLGMEAIYEFDVTDMPVTVAVDSTGTSVHNIGPKEWKDKI 499