BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0023.p (395 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IQM4.1 D8IQM4_HERSS Poly[D(-)-3-hydroxyalkanoate] depolymerase... 734 0.0 A4G3A7.1 A4G3A7_HERAR Poly[D(-)-3-Hydroxybutyrate] depolymerase ... 682 0.0 A6SVW1.1 A6SVW1_JANMA Poly-beta-hydroxyalkanoate depolymerase 673 0.0 F1W1F7.1 F1W1F7_9BURK Poly D(-)-3-Hydroxybutyrate depolymerase 665 0.0 C0INR5.1 C0INR5_9BACT Poly[D(--3-Hydroxybutyrate] depolymerase) 659 0.0 >D8IQM4.1 D8IQM4_HERSS Poly[D(-)-3-hydroxyalkanoate] depolymerase protein EC=3.1.1.75 Length = 411 Score = 734 bits (1896), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/395 (86%), Positives = 376/395 (95%) Query: 1 MQWAETSAKLFSDPVSPLAHTPFAQRIAAGYELLYRLGKDYEKPEFQIESTEINGKTVDI 60 MQWAETSAKLFSDPVSPLAHTPF+QRIAAGYELLYRL K+YEKP+F I++ ++GK+V I Sbjct: 17 MQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKPQFNIDAVPVDGKSVGI 76 Query: 61 IEKIAETKPFCRLIHFRKDLDSKQLAALKQPTVLLVAPLSGHHSTLLRDTVRGLLPNHDV 120 +E++ E KPFCRLIHFRKDL ++Q ALKQPTVL+VAPLSGHHSTLLR+TVR LL HDV Sbjct: 77 VEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHSTLLRETVRALLQEHDV 136 Query: 121 FITDWTDARMVPLDEGAFHLHDYVYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASA 180 +ITDWTDARMVP+++GAFHLHDYVYY+QDFIRTLGPDVHVISVCQPTVPVLAAI+LMASA Sbjct: 137 YITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVCQPTVPVLAAISLMASA 196 Query: 181 NDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYSVPANFPGFGRKVYPGF 240 NDP LPKSMTMMGGPIDARKSPTAVNDLATEKP+SWFENTVIYSVPAN+PGFGRKVYPGF Sbjct: 197 NDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYSVPANYPGFGRKVYPGF 256 Query: 241 LQHAGFVAMNPRRHAESHWDFYMHLRDGDDESAESHRKFYDEYNAVLDMPAEFYLETIKI 300 LQHAGFVAMNPRRHA+SHWDFYMHLRDGDD SAE HRKFYDEYNAVLDMPAEFYLETIK+ Sbjct: 257 LQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYNAVLDMPAEFYLETIKV 316 Query: 301 VFQDFDLPQGTWKIDGKLVRPQDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRK 360 VFQDF+L +GTW+I+G+LVRPQDIKSVALFTIEGELDDISGSGQTQAAQ+LCSSIPK+RK Sbjct: 317 VFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQTQAAQELCSSIPKARK 376 Query: 361 QHFTAPKCGHYGIFSGRRWREVICPKIGEFIKANS 395 QHFT PK GHYGIFSGRRWRE++CPKIGEFI+AN+ Sbjct: 377 QHFTVPKAGHYGIFSGRRWREIVCPKIGEFIRANA 411 >A4G3A7.1 A4G3A7_HERAR Poly[D(-)-3-Hydroxybutyrate] depolymerase (PHB depolymerase) Length = 411 Score = 682 bits (1760), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/395 (80%), Positives = 351/395 (88%) Query: 1 MQWAETSAKLFSDPVSPLAHTPFAQRIAAGYELLYRLGKDYEKPEFQIESTEINGKTVDI 60 M WAE S++LF+DP SPLA+TP+AQRIAAGYELLYRLGK YEKP F IES +NGKTV I Sbjct: 17 MHWAEASSRLFTDPASPLAYTPYAQRIAAGYELLYRLGKQYEKPNFNIESVLVNGKTVAI 76 Query: 61 IEKIAETKPFCRLIHFRKDLDSKQLAALKQPTVLLVAPLSGHHSTLLRDTVRGLLPNHDV 120 +E+ E K FC LIHFRK L +K+ AALKQP VLLVAPLSGHHSTLLRDTVR LL +HDV Sbjct: 77 VEEETEKKSFCTLIHFRKKLSAKEFAALKQPVVLLVAPLSGHHSTLLRDTVRSLLQDHDV 136 Query: 121 FITDWTDARMVPLDEGAFHLHDYVYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASA 180 +ITDWTDARMVPL EG FHLHDYVYYIQDFIR LGPD+HVISVCQPTVPVLAAI+LMAS Sbjct: 137 YITDWTDARMVPLAEGPFHLHDYVYYIQDFIRRLGPDLHVISVCQPTVPVLAAISLMASE 196 Query: 181 NDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYSVPANFPGFGRKVYPGF 240 DP+LPK+MTMMGGPIDARKSPT VNDLA EKP +WFEN VIYSVP ++PGFGRKVYPGF Sbjct: 197 RDPRLPKTMTMMGGPIDARKSPTQVNDLAVEKPHAWFENQVIYSVPPSYPGFGRKVYPGF 256 Query: 241 LQHAGFVAMNPRRHAESHWDFYMHLRDGDDESAESHRKFYDEYNAVLDMPAEFYLETIKI 300 LQHAGF+AMNP RHA+SHWDFYMHLR+GD+ESAE HRKFYDEYNAVLDMPAE+YLETIK Sbjct: 257 LQHAGFIAMNPGRHAQSHWDFYMHLREGDNESAEEHRKFYDEYNAVLDMPAEYYLETIKT 316 Query: 301 VFQDFDLPQGTWKIDGKLVRPQDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRK 360 VFQ+ LP GTW+I+GKLVRPQDIKSVALFTIEGE DDISG+GQT AAQDLCS++P+S K Sbjct: 317 VFQEHRLPLGTWEIEGKLVRPQDIKSVALFTIEGEKDDISGAGQTHAAQDLCSALPQSDK 376 Query: 361 QHFTAPKCGHYGIFSGRRWREVICPKIGEFIKANS 395 Q FTAP+CGHYGIFSGR+WRE+I PKIGEFI S Sbjct: 377 QQFTAPQCGHYGIFSGRKWREIIAPKIGEFITRYS 411 >A6SVW1.1 A6SVW1_JANMA Poly-beta-hydroxyalkanoate depolymerase Length = 411 Score = 673 bits (1737), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/395 (78%), Positives = 349/395 (88%) Query: 1 MQWAETSAKLFSDPVSPLAHTPFAQRIAAGYELLYRLGKDYEKPEFQIESTEINGKTVDI 60 +QWAE S+KLFSDPVSP A+TPFAQ IAAGYEL+YRLGKDY KP F I S I GK V I Sbjct: 17 IQWAEASSKLFSDPVSPFAYTPFAQPIAAGYELMYRLGKDYAKPHFDISSVNIKGKDVKI 76 Query: 61 IEKIAETKPFCRLIHFRKDLDSKQLAALKQPTVLLVAPLSGHHSTLLRDTVRGLLPNHDV 120 IE++AE K FC L+HF+KD+ +K AALKQPT+LLVAPLSGHH+TLLRDTVR LL HDV Sbjct: 77 IEEVAEEKSFCTLLHFKKDMSAKAYAALKQPTILLVAPLSGHHATLLRDTVRSLLVEHDV 136 Query: 121 FITDWTDARMVPLDEGAFHLHDYVYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASA 180 +ITDW DARMVPL EG FHLHDY+YYIQDFIR LGP++HVISVCQPTVPVLAAI+LMAS Sbjct: 137 YITDWRDARMVPLTEGPFHLHDYIYYIQDFIRQLGPNLHVISVCQPTVPVLAAISLMASE 196 Query: 181 NDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYSVPANFPGFGRKVYPGF 240 DP LPKSMTMMGGPID RKSPT VN+LATEKP+SWFEN VI+SVPA++PGFGRKVYPGF Sbjct: 197 RDPHLPKSMTMMGGPIDPRKSPTQVNNLATEKPYSWFENQVIHSVPASYPGFGRKVYPGF 256 Query: 241 LQHAGFVAMNPRRHAESHWDFYMHLRDGDDESAESHRKFYDEYNAVLDMPAEFYLETIKI 300 LQHAGF+AMNP RHA+SHWDFYMHLR+GD+ESAE HRKFYDEYNAVLDMPAE+YLETIK Sbjct: 257 LQHAGFIAMNPGRHAQSHWDFYMHLREGDNESAEEHRKFYDEYNAVLDMPAEYYLETIKT 316 Query: 301 VFQDFDLPQGTWKIDGKLVRPQDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRK 360 VFQ+ LP GTW+++GKLV+PQDIKSVALFTIEGE DDISG GQT AAQDLCSSI KS K Sbjct: 317 VFQEHRLPLGTWEVEGKLVKPQDIKSVALFTIEGERDDISGLGQTHAAQDLCSSIAKSNK 376 Query: 361 QHFTAPKCGHYGIFSGRRWREVICPKIGEFIKANS 395 Q FTAP+CGHYGIFSGR+WRE+ICPKI +FI +S Sbjct: 377 QQFTAPQCGHYGIFSGRKWREIICPKITDFIAKHS 411 >F1W1F7.1 F1W1F7_9BURK Poly D(-)-3-Hydroxybutyrate depolymerase Length = 399 Score = 665 bits (1717), Expect = 0.0, Method: Compositional matrix adjust. Identities = 304/395 (76%), Positives = 347/395 (87%) Query: 1 MQWAETSAKLFSDPVSPLAHTPFAQRIAAGYELLYRLGKDYEKPEFQIESTEINGKTVDI 60 +QWAE SAKLF++PVSPLAHTPF+QRIAAGYEL++RLGKDYEKP+F + ST ING+ V I Sbjct: 5 IQWAEASAKLFTNPVSPLAHTPFSQRIAAGYELMFRLGKDYEKPQFGLTSTTINGEDVGI 64 Query: 61 IEKIAETKPFCRLIHFRKDLDSKQLAALKQPTVLLVAPLSGHHSTLLRDTVRGLLPNHDV 120 +E + KPFC L+HF K+L + AL QP VLLVAPLSGHHSTLLRDTV+ LLP HDV Sbjct: 65 LEAPSTVKPFCTLVHFAKNLTVAERTALNQPKVLLVAPLSGHHSTLLRDTVKALLPGHDV 124 Query: 121 FITDWTDARMVPLDEGAFHLHDYVYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASA 180 +ITDWTDARMVPL GAFHLHDY+YY+QDFIR LGPDVHVISVCQPTVPVLAAI+LMA+A Sbjct: 125 YITDWTDARMVPLAAGAFHLHDYIYYVQDFIRQLGPDVHVISVCQPTVPVLAAISLMATA 184 Query: 181 NDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYSVPANFPGFGRKVYPGF 240 DP LPK+MTMMGGPID RKSPT VN+LAT+KPFSWFENTVIY+VP+N+PG GRKVYPGF Sbjct: 185 KDPMLPKTMTMMGGPIDPRKSPTEVNNLATKKPFSWFENTVIYTVPSNYPGAGRKVYPGF 244 Query: 241 LQHAGFVAMNPRRHAESHWDFYMHLRDGDDESAESHRKFYDEYNAVLDMPAEFYLETIKI 300 LQHAGFVAMNP RHA+SHWDFYM LR GDD SAE HRKFYDEYNAVLDMP+E+YLETIK Sbjct: 245 LQHAGFVAMNPDRHAQSHWDFYMQLRKGDDASAEEHRKFYDEYNAVLDMPSEYYLETIKT 304 Query: 301 VFQDFDLPQGTWKIDGKLVRPQDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRK 360 VFQDF+LP GTW+++GKLVRPQDIK+VAL T+EGELDDISG+GQTQAA LCS IP + Sbjct: 305 VFQDFNLPLGTWEVEGKLVRPQDIKTVALLTVEGELDDISGAGQTQAAHTLCSGIPADMQ 364 Query: 361 QHFTAPKCGHYGIFSGRRWREVICPKIGEFIKANS 395 + + A CGHYGIF+GRRWRE+ICPK+G FIKA+S Sbjct: 365 EDYIAEGCGHYGIFAGRRWRELICPKVGAFIKAHS 399 >C0INR5.1 C0INR5_9BACT Poly[D(--3-Hydroxybutyrate] depolymerase) Length = 411 Score = 659 bits (1700), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/395 (78%), Positives = 352/395 (89%) Query: 1 MQWAETSAKLFSDPVSPLAHTPFAQRIAAGYELLYRLGKDYEKPEFQIESTEINGKTVDI 60 MQWA+ S+KLF++PVSPLAHTPF+QRIAAGYEL+YRLGKDYEKP F+I+ +NG+ V I Sbjct: 17 MQWADASSKLFTNPVSPLAHTPFSQRIAAGYELMYRLGKDYEKPAFEIDDALVNGEAVGI 76 Query: 61 IEKIAETKPFCRLIHFRKDLDSKQLAALKQPTVLLVAPLSGHHSTLLRDTVRGLLPNHDV 120 IE++ KPFCRLIHF+KDLD K++ ALKQPTVLLVAPLSGHHSTLLRDTVRGLL +HDV Sbjct: 77 IEQVVVNKPFCRLIHFKKDLDDKKIKALKQPTVLLVAPLSGHHSTLLRDTVRGLLVDHDV 136 Query: 121 FITDWTDARMVPLDEGAFHLHDYVYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASA 180 +ITDWTDARMVPL EGAFHL DY++Y+QDFIR LGPDVHVISVCQPTVPVLAAI+LMAS Sbjct: 137 YITDWTDARMVPLSEGAFHLDDYIFYVQDFIRQLGPDVHVISVCQPTVPVLAAISLMASE 196 Query: 181 NDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYSVPANFPGFGRKVYPGF 240 NDPKLPK+MTMMGGPID R+SPT VN+LATEK FSWFENTVIY+VP N+PGFGRKVYPGF Sbjct: 197 NDPKLPKTMTMMGGPIDPRRSPTQVNNLATEKKFSWFENTVIYAVPPNYPGFGRKVYPGF 256 Query: 241 LQHAGFVAMNPRRHAESHWDFYMHLRDGDDESAESHRKFYDEYNAVLDMPAEFYLETIKI 300 LQHAGF+AMNP RHA+SH +FYMHL +GDDE A+SHRKFYDEYNAVLDMPAE+YL+TIK Sbjct: 257 LQHAGFIAMNPGRHAQSHREFYMHLVEGDDEPADSHRKFYDEYNAVLDMPAEYYLDTIKT 316 Query: 301 VFQDFDLPQGTWKIDGKLVRPQDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRK 360 VFQDF LP+G W + GKLVRPQDI +VALFT+EGELDDISG+GQTQAA DLCS IP + Sbjct: 317 VFQDFSLPKGEWVVGGKLVRPQDITTVALFTVEGELDDISGAGQTQAAHDLCSGIPADMQ 376 Query: 361 QHFTAPKCGHYGIFSGRRWREVICPKIGEFIKANS 395 Q F AP GHYGIFSGRRWRE++CPKI EFIK + Sbjct: 377 QDFVAPGAGHYGIFSGRRWREMVCPKITEFIKKHG 411