BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0188.p (275 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IYH6.1 D8IYH6_HERSS 3-methyl-2-oxobutanoate hydroxymethyltrans... 448 e-124 A4G8C6.1 A4G8C6_HERAR 3-methyl-2-oxobutanoate hydroxymethyltrans... 411 e-113 C3X542.1 C3X542_OXAFO 3-methyl-2-oxobutanoate hydroxymethyltrans... 404 e-111 A6T221.1 PANB_JANMA 3-methyl-2-oxobutanoate hydroxymethyltransfe... 395 e-108 F1W3V5.1 F1W3V5_9BURK 3-methyl-2-oxobutanoate hydroxymethyltrans... 394 e-108 >D8IYH6.1 D8IYH6_HERSS 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 275 Score = 448 bits (1152), Expect = e-124, Method: Compositional matrix adjust. Identities = 213/275 (77%), Positives = 241/275 (87%) Query: 1 MAAYLQDVPATRKKAVTIHTLQAMRDKGDKITMLTCYDASFSALMDRSGVDTVLIGDSLG 60 MA YLQ+ A R K VT HTLQAMR KGDKI+MLTCYDASF+ LM+R+G+DTVLIGDSLG Sbjct: 1 MAGYLQESGAARAKPVTTHTLQAMRAKGDKISMLTCYDASFATLMERAGLDTVLIGDSLG 60 Query: 61 MVCQGHQSTLPVTVTDIAYHTASVARGNQSMLIIADMPFGAYGSPLAAFDNAVKLMQAGA 120 MVCQGH STLPVT+ D+AYHTA+VARG ++MLIIAD+PFG Y + AF NA LMQAGA Sbjct: 61 MVCQGHDSTLPVTIQDVAYHTAAVARGTRTMLIIADLPFGTYPTREEAFRNAALLMQAGA 120 Query: 121 HMVKIEGGAWLADTIRLLTERGIPVCAHLGLTPQSVHQMGGFKVQGKTLEGAELLHADAR 180 M+K+EGG WLADTI+ LTERGIPVCAH+GLTPQSVHQ+GGFKVQGKT +GA LHADA Sbjct: 121 QMIKLEGGVWLADTIKFLTERGIPVCAHIGLTPQSVHQLGGFKVQGKTSDGATQLHADAL 180 Query: 181 AVQAAGASLLVLEAIPATLGADVTAQLRIPTIGIGAGPDCSGQVLVMHDMLGVFPGHKAR 240 AVQ AGASL+V+EA+PATLG +VT +L IPTIGIGAGPDCSGQVLVMHDMLGVFPGHK+R Sbjct: 181 AVQQAGASLVVIEAVPATLGKEVTDRLSIPTIGIGAGPDCSGQVLVMHDMLGVFPGHKSR 240 Query: 241 FVKNFMDGAASVEEAVRGYVAAVKDGSFPAAEHCF 275 FV+NFMDGA+ +EEAVR YVAAVKDGSFPA EHCF Sbjct: 241 FVRNFMDGASGIEEAVRRYVAAVKDGSFPAQEHCF 275 >A4G8C6.1 A4G8C6_HERAR 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 276 Score = 411 bits (1056), Expect = e-113, Method: Compositional matrix adjust. Identities = 196/276 (71%), Positives = 231/276 (83%), Gaps = 1/276 (0%) Query: 1 MAAYLQDVP-ATRKKAVTIHTLQAMRDKGDKITMLTCYDASFSALMDRSGVDTVLIGDSL 59 M +YLQ+ A R KAVT+ L A+R KG+KITMLT YDASF++LMD GV+T+L+GDSL Sbjct: 1 MTSYLQENETAGRNKAVTVPALFALRAKGEKITMLTAYDASFASLMDMCGVETLLVGDSL 60 Query: 60 GMVCQGHQSTLPVTVTDIAYHTASVARGNQSMLIIADMPFGAYGSPLAAFDNAVKLMQAG 119 GMVCQGH STLPVT+ DIAYHTA VARGN++ L++ADMPFG+Y +P +AF NAVKL+QAG Sbjct: 61 GMVCQGHSSTLPVTIEDIAYHTACVARGNKTALLLADMPFGSYATPESAFQNAVKLIQAG 120 Query: 120 AHMVKIEGGAWLADTIRLLTERGIPVCAHLGLTPQSVHQMGGFKVQGKTLEGAELLHADA 179 A MVKIEGGAW+ DT+R L ER IPVCAHLGLTPQSVH+ GG+KVQGKT E A+ L DA Sbjct: 121 AQMVKIEGGAWVLDTVRFLVEREIPVCAHLGLTPQSVHRFGGYKVQGKTAEAAQQLKDDA 180 Query: 180 RAVQAAGASLLVLEAIPATLGADVTAQLRIPTIGIGAGPDCSGQVLVMHDMLGVFPGHKA 239 A+QAAGASLL+LEAIPA+LG +VT L +PTIGIGAGPDCSGQVLVMHD +GVFPG KA Sbjct: 181 LALQAAGASLLILEAIPASLGKEVTDILTMPTIGIGAGPDCSGQVLVMHDFIGVFPGKKA 240 Query: 240 RFVKNFMDGAASVEEAVRGYVAAVKDGSFPAAEHCF 275 RFVKNFM+G S+E AVR Y+ VK+G+FPA EHCF Sbjct: 241 RFVKNFMEGQTSIEGAVRAYIREVKEGTFPAPEHCF 276 >C3X542.1 C3X542_OXAFO 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 270 Score = 404 bits (1037), Expect = e-111, Method: Compositional matrix adjust. Identities = 183/275 (66%), Positives = 229/275 (83%), Gaps = 5/275 (1%) Query: 1 MAAYLQDVPATRKKAVTIHTLQAMRDKGDKITMLTCYDASFSALMDRSGVDTVLIGDSLG 60 MA YLQ+ +K+VT HTL A+ +K +KITMLTCYDASF++LMD+ GV+ +LIGDSLG Sbjct: 1 MATYLQE-----RKSVTSHTLAALYNKHEKITMLTCYDASFASLMDQCGVEILLIGDSLG 55 Query: 61 MVCQGHQSTLPVTVTDIAYHTASVARGNQSMLIIADMPFGAYGSPLAAFDNAVKLMQAGA 120 MVCQG STLPV++ ++AYHT VARGN++ +I++DMPFG Y +P +A+DNA +L++AGA Sbjct: 56 MVCQGKDSTLPVSIEEVAYHTECVARGNKTAMIVSDMPFGTYATPESAYDNAAELIKAGA 115 Query: 121 HMVKIEGGAWLADTIRLLTERGIPVCAHLGLTPQSVHQMGGFKVQGKTLEGAELLHADAR 180 HMVKIEGG WL DT+R LTER IPVC+HLGLTPQSVHQ GG++VQGKT E A+ + DA+ Sbjct: 116 HMVKIEGGRWLEDTVRFLTERAIPVCSHLGLTPQSVHQFGGYRVQGKTSEDAKRILEDAK 175 Query: 181 AVQAAGASLLVLEAIPATLGADVTAQLRIPTIGIGAGPDCSGQVLVMHDMLGVFPGHKAR 240 +Q AGASL VLEA+PA LG ++T L +PTIGIGAGPDCSGQVLVMHDML V+PG KAR Sbjct: 176 ILQNAGASLYVLEAVPAILGKEITEALNVPTIGIGAGPDCSGQVLVMHDMLNVYPGRKAR 235 Query: 241 FVKNFMDGAASVEEAVRGYVAAVKDGSFPAAEHCF 275 FV+NFM+G +S+E+A++ YV AVKDGSFPA EHCF Sbjct: 236 FVRNFMEGQSSIEDAIKAYVRAVKDGSFPAPEHCF 270 >A6T221.1 PANB_JANMA 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 272 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 183/275 (66%), Positives = 227/275 (82%), Gaps = 3/275 (1%) Query: 1 MAAYLQDVPATRKKAVTIHTLQAMRDKGDKITMLTCYDASFSALMDRSGVDTVLIGDSLG 60 M++YLQ+ R KAVTI +L AM+++G+KITMLT YDASF+++MD GV+ +LIGDSLG Sbjct: 1 MSSYLQEA---RTKAVTIPSLLAMKERGEKITMLTAYDASFASMMDTCGVEMILIGDSLG 57 Query: 61 MVCQGHQSTLPVTVTDIAYHTASVARGNQSMLIIADMPFGAYGSPLAAFDNAVKLMQAGA 120 MVCQGH STLPVT+ D+AYHTA VARG+++ +++DMPFG+Y + AF NAVKL+QAGA Sbjct: 58 MVCQGHNSTLPVTIEDVAYHTACVARGSKTAFVMSDMPFGSYATKEEAFKNAVKLVQAGA 117 Query: 121 HMVKIEGGAWLADTIRLLTERGIPVCAHLGLTPQSVHQMGGFKVQGKTLEGAELLHADAR 180 MVKIEGGAW+ADT+R LTER IPV HLGLTPQ VHQ GG+KVQGKT E AE + ++A Sbjct: 118 QMVKIEGGAWMADTVRFLTERAIPVMVHLGLTPQFVHQFGGYKVQGKTAEAAEKMKSEAL 177 Query: 181 AVQAAGASLLVLEAIPATLGADVTAQLRIPTIGIGAGPDCSGQVLVMHDMLGVFPGHKAR 240 +Q AGA +L+LEAIPA+LG ++T +++PTIGIGAGPDCSGQVLV+HD + VFPG KAR Sbjct: 178 LLQEAGADMLLLEAIPASLGKELTELVKMPTIGIGAGPDCSGQVLVLHDYINVFPGKKAR 237 Query: 241 FVKNFMDGAASVEEAVRGYVAAVKDGSFPAAEHCF 275 FV+NFM+G S+E A R YVAAVKDGSFPA EHCF Sbjct: 238 FVRNFMEGQTSIEGATRAYVAAVKDGSFPAPEHCF 272 >F1W3V5.1 F1W3V5_9BURK 3-methyl-2-oxobutanoate hydroxymethyltransferase Length = 278 Score = 394 bits (1011), Expect = e-108, Method: Compositional matrix adjust. Identities = 192/276 (69%), Positives = 231/276 (83%), Gaps = 1/276 (0%) Query: 1 MAAYLQ-DVPATRKKAVTIHTLQAMRDKGDKITMLTCYDASFSALMDRSGVDTVLIGDSL 59 MA YLQ + A R K VTIHTL A+R G+KITMLTCYDASFSALMDR G+D +L+GDSL Sbjct: 3 MAGYLQENQSAGRNKPVTIHTLAALRQAGEKITMLTCYDASFSALMDRCGIDIMLVGDSL 62 Query: 60 GMVCQGHQSTLPVTVTDIAYHTASVARGNQSMLIIADMPFGAYGSPLAAFDNAVKLMQAG 119 GMV QGH STLPVTV D+AYHTA VARGN + +++AD+PFG+Y +P +A+ NAV LMQAG Sbjct: 63 GMVSQGHDSTLPVTVADMAYHTACVARGNHTAMLMADLPFGSYATPESAYANAVLLMQAG 122 Query: 120 AHMVKIEGGAWLADTIRLLTERGIPVCAHLGLTPQSVHQMGGFKVQGKTLEGAELLHADA 179 A +VK+EGG WL +T+R L+ERG+PVCAHLGLTPQSVHQ+GG+KVQGK++ A+ L +DA Sbjct: 123 AQIVKLEGGQWLVETVRFLSERGVPVCAHLGLTPQSVHQLGGYKVQGKSVASADQLKSDA 182 Query: 180 RAVQAAGASLLVLEAIPATLGADVTAQLRIPTIGIGAGPDCSGQVLVMHDMLGVFPGHKA 239 A+QAAGA++++ EAIPA LG + TA L IPTIGIGAGPDCSGQVLVMHDMLG FPG KA Sbjct: 183 LALQAAGAAVMLFEAIPAALGKEATALLAIPTIGIGAGPDCSGQVLVMHDMLGAFPGRKA 242 Query: 240 RFVKNFMDGAASVEEAVRGYVAAVKDGSFPAAEHCF 275 RFVKNFMDG S++ AV Y+ AVK+G+FPAAEHCF Sbjct: 243 RFVKNFMDGQGSIDGAVSAYIKAVKEGTFPAAEHCF 278