BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0038.p (446 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IZC2.1 D8IZC2_HERSS tRNA modifying 2-methylthioadenine synthet... 805 0.0 A6SV24.1 MIAB_JANMA (Dimethylallyl)adenosine tRNA methylthiotran... 803 0.0 A4G267.1 MIAB_HERAR (Dimethylallyl)adenosine tRNA methylthiotran... 802 0.0 B2JD88.1 MIAB_BURP8 (Dimethylallyl)adenosine tRNA methylthiotran... 721 0.0 C5ZK26.1 C5ZK26_BURPS tRNA-i(6)A37 thiotransferase enzyme MiaB 719 0.0 >D8IZC2.1 D8IZC2_HERSS tRNA modifying 2-methylthioadenine synthetase protein Length = 446 Score = 805 bits (2078), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/446 (84%), Positives = 417/446 (93%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNLSDGLIKTDRPEDADVILLNTCSVREKAQEKVF 60 MQKKVFIKTFGCQMNEYDSDKMADVLN SDGLIKTDRPE+ADVILLNTCS+REKAQEKVF Sbjct: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 Query: 61 SDLGRLRELKKKNPDLLIGVGGCVASQEGEAIVKRAPFVDMVFGPQTLHRLPQMISERRF 120 SDLGRLR LKK NP LLIGVGGCVASQEGEAI+KRAPFVDMVFGPQTLHRLPQMISERR+ Sbjct: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 Query: 121 TGRPQVDISFPEIEKFDHMPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GRPQVDISFPEIEKFDH+PPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLAAQGVKEITLLGQNVNAFRGVMEDGEIADFALLIEYIAEMPGIQRIRFVTSH 240 VLTEVAGLA QGVKEI LLGQNVNA+RGVMEDGEIADFALLIEYIAE+PGI+RIRFVTSH Sbjct: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 Query: 241 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSVLRRLREVRPNI 300 PKEF+QRLIDTYAKVPKL +HLYLPAQHGSDR LAAMKRGYT+LEYKSV+RR+R+VRP+I Sbjct: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 Query: 301 TISSDFIVGFPGETDEDFNALMKLITDIGFDSSFSFVFSPRPGTPAANLADDTPADVKLK 360 T+ SDFIVGFPGET+EDF ALMKL+ D+GFD+SFSF+FS RPGTPAANL DDTP +VKL Sbjct: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 Query: 361 RLQHLQAVVQDNASKISQAMLGTVQRILVEGPSKKDPNELQGRTENNRVVNFDGGPNGAR 420 RLQ LQA + DNA++IS A +GTVQR+LVEG SK+ +ELQGRTE+NRVVNFDGGPNG R Sbjct: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 Query: 421 LIGEMIDVTIVQTFAFSLRGEIIVKQ 446 LIG++I+V+I + F FSLRGEI+ + Sbjct: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 >A6SV24.1 MIAB_JANMA (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 446 Score = 803 bits (2074), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/446 (85%), Positives = 415/446 (93%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNLSDGLIKTDRPEDADVILLNTCSVREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKM DVLN S+GLIKTD PEDADVILLNTCSVREKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMVDVLNASEGLIKTDTPEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRELKKKNPDLLIGVGGCVASQEGEAIVKRAPFVDMVFGPQTLHRLPQMISERRF 120 SDLGRLRELK NPDL+IGVGGCVASQEG+AIVKRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKFNNPDLVIGVGGCVASQEGDAIVKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 TGRPQVDISFPEIEKFDHMPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 TGR QVDISFPEIEKFDHMPPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLAAQGVKEITLLGQNVNAFRGVMEDGEIADFALLIEYIAEMPGIQRIRFVTSH 240 VL EVAGL AQGVKEITLLGQNVNA+RG M DGEIADFALLIEYIAE+ GI+RIRFVTSH Sbjct: 181 VLAEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAELEGIERIRFVTSH 240 Query: 241 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSVLRRLREVRPNI 300 PKEFTQRLID YAKVPKLV+HLYLPAQHGSDRILAAMKRGYTSLEYKS+LRRLREVRPNI Sbjct: 241 PKEFTQRLIDAYAKVPKLVNHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLREVRPNI 300 Query: 301 TISSDFIVGFPGETDEDFNALMKLITDIGFDSSFSFVFSPRPGTPAANLADDTPADVKLK 360 +ISSDFIVGFPGETD DF A+MKLI DIG+D+SFSF+FSPRPGTPAANL DDTP +VKL+ Sbjct: 301 SISSDFIVGFPGETDADFEAMMKLINDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLQ 360 Query: 361 RLQHLQAVVQDNASKISQAMLGTVQRILVEGPSKKDPNELQGRTENNRVVNFDGGPNGAR 420 RLQ LQAV+ N + S M+GTVQRILVEGPSKKDP+ELQGRTENNRVVNF G +GAR Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGTVQRILVEGPSKKDPDELQGRTENNRVVNFAAGEHGAR 420 Query: 421 LIGEMIDVTIVQTFAFSLRGEIIVKQ 446 LIG+M+DV IVQ+FA++LRGEIIVKQ Sbjct: 421 LIGQMVDVNIVQSFAYTLRGEIIVKQ 446 >A4G267.1 MIAB_HERAR (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 446 Score = 802 bits (2071), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/446 (84%), Positives = 414/446 (92%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNLSDGLIKTDRPEDADVILLNTCSVREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKMADVLN SDGLIKTD+ EDADVILLNTCSVREKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMADVLNASDGLIKTDKAEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRELKKKNPDLLIGVGGCVASQEGEAIVKRAPFVDMVFGPQTLHRLPQMISERRF 120 SDLGRLRELK PDL+IGVGGCVASQEG+AI+KRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKLLKPDLMIGVGGCVASQEGDAIIKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 TGRPQVDISFPEIEKFDHMPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 TGR QVDISFPEIEKFDHMPPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRF+D Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFDD 180 Query: 181 VLTEVAGLAAQGVKEITLLGQNVNAFRGVMEDGEIADFALLIEYIAEMPGIQRIRFVTSH 240 VLTEVAGL AQGVKEITLLGQNVNA+RG M DGEIADFALLIEYIAEM GI+RIR+VTSH Sbjct: 181 VLTEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAEMEGIERIRYVTSH 240 Query: 241 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSVLRRLREVRPNI 300 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKS+LRRLR VRPNI Sbjct: 241 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLRAVRPNI 300 Query: 301 TISSDFIVGFPGETDEDFNALMKLITDIGFDSSFSFVFSPRPGTPAANLADDTPADVKLK 360 +ISSDFIVGFPGETDEDF A+MKLI DIG+D+SFSF+FSPRPGTPAANLADDTP +VKLK Sbjct: 301 SISSDFIVGFPGETDEDFEAMMKLIDDIGYDNSFSFIFSPRPGTPAANLADDTPHEVKLK 360 Query: 361 RLQHLQAVVQDNASKISQAMLGTVQRILVEGPSKKDPNELQGRTENNRVVNFDGGPNGAR 420 RLQ LQAV+ N + S M+G+VQRILVEGPSKKD +ELQGRTENNRVVNF GPN A Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGSVQRILVEGPSKKDADELQGRTENNRVVNFSAGPNAAH 420 Query: 421 LIGEMIDVTIVQTFAFSLRGEIIVKQ 446 LIG+M++V I Q+ A++LRGEI+VKQ Sbjct: 421 LIGQMVNVNITQSHAYTLRGEIVVKQ 446 >B2JD88.1 MIAB_BURP8 (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 456 Score = 721 bits (1861), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/447 (74%), Positives = 396/447 (88%), Gaps = 3/447 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNLSDGLIKTDRPEDADVILLNTCSVREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVL ++GL+KTD PEDADVIL NTCSVREKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRELKKKNPDLLIGVGGCVASQEGEAIVKRAPFVDMVFGPQTLHRLPQMISERRF 120 SDLGR+RELK+ NP+LLIGVGGCVASQEG +IV RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEANPNLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 TGRPQVDISFPEIEKFDHMPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GRPQVDI+FPEIEKFDH+PPAR++G +A+VSIMEGCSKYCSYCVVPYTRGEEVSR +D Sbjct: 121 SGRPQVDITFPEIEKFDHLPPARIDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDD 180 Query: 181 VLTEVAGLAAQGVKEITLLGQNVNAFRGVMEDG--EIADFALLIEYIAEMPGIQRIRFVT 238 VLTE+AGLA QGV+E+TLLGQNVNA+RG M G EIADFA LIEY+A++PGI+RIR+ T Sbjct: 181 VLTEIAGLADQGVREVTLLGQNVNAYRGAMTLGATEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSVLRRLREVRP 298 SHPKEFTQRLIDTYAKVPKLV HL+LP QHGSDRIL AMKRGYT LEYKSV+R+LR +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 NITISSDFIVGFPGETDEDFNALMKLITDIGFDSSFSFVFSPRPGTPAANLADDTPADVK 358 ++++S+D IVGFPGET+EDF+ +M+L+ D+ +D+SFSF++SPRPGTPAANL DDTP +VK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMQLVHDMSYDTSFSFIYSPRPGTPAANLQDDTPREVK 360 Query: 359 LKRLQHLQAVVQDNASKISQAMLGTVQRILVEGPSKKDPNELQGRTENNRVVNFDGG-PN 417 L+RLQHLQA +++N +ISQAM+G V+RILVE P++KDPNEL GRTENNRVVNF Sbjct: 361 LRRLQHLQATIEENVQRISQAMVGKVERILVERPARKDPNELAGRTENNRVVNFPAPVET 420 Query: 418 GARLIGEMIDVTIVQTFAFSLRGEIIV 444 ARLIG+MIDV IV + SLRGE+++ Sbjct: 421 HARLIGQMIDVKIVHAYPHSLRGELVM 447 >C5ZK26.1 C5ZK26_BURPS tRNA-i(6)A37 thiotransferase enzyme MiaB Length = 457 Score = 719 bits (1855), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/450 (74%), Positives = 394/450 (87%), Gaps = 5/450 (1%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNLSDGLIKTDRPEDADVILLNTCSVREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLN ++GL KTD PEDAD+IL NTCSVREKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRELKKKNPDLLIGVGGCVASQEGEAIVKRAPFVDMVFGPQTLHRLPQMISERRF 120 SDLGR+RELK+ PDLLIGVGGCVASQEG +IV RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRE 120 Query: 121 TGRPQVDISFPEIEKFDHMPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GR QVDI+FPEIEKFDH+PPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLAAQGVKEITLLGQNVNAFRGVMEDG--EIADFALLIEYIAEMPGIQRIRFVT 238 VLTEVAGLA QGV+E+TLLGQNVNA+RG + G EIADFA LIEY+A++PGI+RIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSVLRRLREVRP 298 SHPKEFTQRL+D YAKVPKLVDHL+LP QHGSDRIL AMKRGYT LEYKSV+R+LR +RP Sbjct: 241 SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 NITISSDFIVGFPGETDEDFNALMKLITDIGFDSSFSFVFSPRPGTPAANLADDTPADVK 358 N+++S+D IVGFPGETD DF+ M L+ ++ +D+SFSF++SPRPGTPAANLADDTP ++K Sbjct: 301 NLSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELK 360 Query: 359 LKRLQHLQAVVQDNASKISQAMLGTVQRILVEGPSKKDPNELQGRTENNRVVNFDGGPNG 418 LKRLQHLQA +++N ++ISQ+MLG V+RILVEGPS+KDPNEL GRTENNRVVNF P+ Sbjct: 361 LKRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFP-APSA 419 Query: 419 A--RLIGEMIDVTIVQTFAFSLRGEIIVKQ 446 A RLIG+MIDV I + SLRGE+++ Sbjct: 420 AHPRLIGQMIDVKINHAYPHSLRGELVLAH 449