BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0181.p (432 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IZ47.1 D8IZ47_HERSS tRNA uridine 5-carboxymethylaminomethyl mo... 798 0.0 A6T482.1 MNMG_JANMA tRNA uridine 5-carboxymethylaminomethyl modi... 769 0.0 A4GAI2.2 MNMG_HERAR tRNA uridine 5-carboxymethylaminomethyl modi... 756 0.0 F1VTT1.1 F1VTT1_9BURK tRNA uridine 5-carboxymethylaminomethyl mo... 739 0.0 E2SXC4.1 E2SXC4_9RALS tRNA uridine 5-carboxymethylaminomethyl mo... 674 0.0 >D8IZ47.1 D8IZ47_HERSS tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG Length = 642 Score = 798 bits (2061), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/432 (87%), Positives = 408/432 (94%), Gaps = 3/432 (0%) Query: 1 VLEEQPGDLDPIPVFSVMGNVGMHPQQMPCWITHTNTQTHDIIRAGLDRSPMYTGVIEGV 60 +LEEQPGDLDP+PVFSVMGN MHP+QMPCWITHTN++THDIIR GLDRSPMYTGVIEGV Sbjct: 214 ILEEQPGDLDPVPVFSVMGNAAMHPRQMPCWITHTNSRTHDIIRGGLDRSPMYTGVIEGV 273 Query: 61 GPRYCPSIEDKIHRFAGKDSHQIYLEPEGLTTNEFYPNGISTSLPFDVQLALVRSMRGME 120 GPRYCPSIEDKIHRFA KDSHQIYLEPEGLTT+EFYPNGISTSLPFDVQL LVRSM+GME Sbjct: 274 GPRYCPSIEDKIHRFASKDSHQIYLEPEGLTTHEFYPNGISTSLPFDVQLELVRSMKGME 333 Query: 121 NAHILRPGYAIEYDYFDPRGLKSSLETRQIQGLFFAGQINGTTGYEEAAAQGMLAGLNAA 180 NAHILRPGYAIEYDY+DPRGLKSSLETRQ+QGLFFAGQINGTTGYEEAAAQGMLAG+NAA Sbjct: 334 NAHILRPGYAIEYDYYDPRGLKSSLETRQVQGLFFAGQINGTTGYEEAAAQGMLAGINAA 393 Query: 181 LQTQGRDSWTPRRDEAYLGVLVDDLITQGVLEPYRMFTSRAEYRLSLREDNADLRLTEIG 240 LQTQGR++WTPRRDEAYLGVLVDDL+TQGV EPYRMFTSRAEYRLSLREDNADLRLTEIG Sbjct: 394 LQTQGREAWTPRRDEAYLGVLVDDLVTQGVQEPYRMFTSRAEYRLSLREDNADLRLTEIG 453 Query: 241 RQLGCVGDAQWEMFEKKREAVAREMERLKSTWVNPRILAEAEAERVLGKAIDHEYSLFDL 300 R+LGCVGDAQWE F++KREAV+REMERLKSTWVNPRILA AEAERVLGKAI+ EY+L DL Sbjct: 454 RELGCVGDAQWEAFDRKREAVSREMERLKSTWVNPRILAAAEAERVLGKAIEREYNLLDL 513 Query: 301 LRRPNVTYDSLMTLAGADGQALAGPGVSDDPVREQIEIQIKYAGYIDRQAKEIGRQEHNE 360 LRRPNV+YDSLM L G +GQALAGPG+ D PVREQ+EIQ+KYAGYI RQA EI RQ+HNE Sbjct: 514 LRRPNVSYDSLMGLTGTEGQALAGPGIEDGPVREQVEIQVKYAGYIARQADEISRQDHNE 573 Query: 361 SLKLPADLDYMEVQALSIEVRQKLNKHKPETLGQASRISGVTPAALSLLLVHLKKGGLKN 420 +LKLPADLDYM+V+ALSIEVRQKLNKH+PETLGQASRISGVTPAALSLLLVHLKKGGLK Sbjct: 574 NLKLPADLDYMDVKALSIEVRQKLNKHRPETLGQASRISGVTPAALSLLLVHLKKGGLK- 632 Query: 421 VAEASANHNEAA 432 A+A N+AA Sbjct: 633 --PATATDNQAA 642 >A6T482.1 MNMG_JANMA tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG Length = 645 Score = 769 bits (1986), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/432 (83%), Positives = 400/432 (92%) Query: 1 VLEEQPGDLDPIPVFSVMGNVGMHPQQMPCWITHTNTQTHDIIRAGLDRSPMYTGVIEGV 60 V++EQPGDLDPIPVFSVMG+V MHP+QMPCWITHTN +THD+IR+GLDRSPMYTGVIEGV Sbjct: 214 VMQEQPGDLDPIPVFSVMGSVAMHPKQMPCWITHTNEKTHDLIRSGLDRSPMYTGVIEGV 273 Query: 61 GPRYCPSIEDKIHRFAGKDSHQIYLEPEGLTTNEFYPNGISTSLPFDVQLALVRSMRGME 120 GPRYCPSIEDKIHRFAGKDSHQI+LEPEGLTTNEFYPNGISTSLPFD+QLALV+SMRG+E Sbjct: 274 GPRYCPSIEDKIHRFAGKDSHQIFLEPEGLTTNEFYPNGISTSLPFDIQLALVQSMRGLE 333 Query: 121 NAHILRPGYAIEYDYFDPRGLKSSLETRQIQGLFFAGQINGTTGYEEAAAQGMLAGLNAA 180 +AHILRPGYAIEYDYFDPRGLKS+LET+ IQGLFFAGQINGTTGYEEAAAQGMLAG+NAA Sbjct: 334 HAHILRPGYAIEYDYFDPRGLKSTLETKVIQGLFFAGQINGTTGYEEAAAQGMLAGINAA 393 Query: 181 LQTQGRDSWTPRRDEAYLGVLVDDLITQGVLEPYRMFTSRAEYRLSLREDNADLRLTEIG 240 LQTQ RD+WTPRRDEAYLGV+VDDLIT+GVLEPYRMFTSRAEYRLSLREDNAD+RLTEIG Sbjct: 394 LQTQERDAWTPRRDEAYLGVMVDDLITKGVLEPYRMFTSRAEYRLSLREDNADMRLTEIG 453 Query: 241 RQLGCVGDAQWEMFEKKREAVAREMERLKSTWVNPRILAEAEAERVLGKAIDHEYSLFDL 300 RQLGCVGDAQW +FE KREAVARE+ERL+STWV+PRILAEAEAERVLGK I+ EY+L DL Sbjct: 454 RQLGCVGDAQWAVFETKREAVARELERLRSTWVSPRILAEAEAERVLGKGIEREYALADL 513 Query: 301 LRRPNVTYDSLMTLAGADGQALAGPGVSDDPVREQIEIQIKYAGYIDRQAKEIGRQEHNE 360 LRRPNV+Y++LM+L G DGQ LAGPGV++D VREQ+EIQ+KYAGYIDRQAKE+ R ++ E Sbjct: 514 LRRPNVSYETLMSLKGVDGQDLAGPGVAEDAVREQVEIQLKYAGYIDRQAKEVERHDYYE 573 Query: 361 SLKLPADLDYMEVQALSIEVRQKLNKHKPETLGQASRISGVTPAALSLLLVHLKKGGLKN 420 +LKLP + DYMEV+ LS EVRQKL KHKPETLGQASRISGVTPAA+SLLLVHLKKGG K Sbjct: 574 NLKLPQEFDYMEVKGLSFEVRQKLCKHKPETLGQASRISGVTPAAISLLLVHLKKGGFKE 633 Query: 421 VAEASANHNEAA 432 A AA Sbjct: 634 FAALPPTSEAAA 645 >A4GAI2.2 MNMG_HERAR tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG Length = 645 Score = 756 bits (1951), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/432 (81%), Positives = 395/432 (91%) Query: 1 VLEEQPGDLDPIPVFSVMGNVGMHPQQMPCWITHTNTQTHDIIRAGLDRSPMYTGVIEGV 60 V+ EQPGDLDPIPVFSVMG+V MHPQQMPCWITHTN +TH++IR GLDRSPMYTG+IEGV Sbjct: 214 VMTEQPGDLDPIPVFSVMGSVAMHPQQMPCWITHTNEKTHELIRGGLDRSPMYTGMIEGV 273 Query: 61 GPRYCPSIEDKIHRFAGKDSHQIYLEPEGLTTNEFYPNGISTSLPFDVQLALVRSMRGME 120 GPRYCPSIEDKIHRFAGK+SHQI+LEPEGLTTNE+YPNGISTSLPFDVQLALV+SMRG+E Sbjct: 274 GPRYCPSIEDKIHRFAGKESHQIFLEPEGLTTNEYYPNGISTSLPFDVQLALVQSMRGLE 333 Query: 121 NAHILRPGYAIEYDYFDPRGLKSSLETRQIQGLFFAGQINGTTGYEEAAAQGMLAGLNAA 180 +AHILRPGYAIEYDYFDPRGLK++LET+ IQGLFFAGQINGTTGYEEAAAQGMLAG+NAA Sbjct: 334 HAHILRPGYAIEYDYFDPRGLKATLETKVIQGLFFAGQINGTTGYEEAAAQGMLAGINAA 393 Query: 181 LQTQGRDSWTPRRDEAYLGVLVDDLITQGVLEPYRMFTSRAEYRLSLREDNADLRLTEIG 240 LQ Q RD+WTPRRDEAYLGVLVDDLITQGVLEPYRMFTSRAEYRLSLREDNAD+RLT++G Sbjct: 394 LQIQERDAWTPRRDEAYLGVLVDDLITQGVLEPYRMFTSRAEYRLSLREDNADMRLTDVG 453 Query: 241 RQLGCVGDAQWEMFEKKREAVAREMERLKSTWVNPRILAEAEAERVLGKAIDHEYSLFDL 300 RQLGCVGD QW +FE KREAVARE+ERL+STWV+PRILAEAEAERVLGK I+ EY+L DL Sbjct: 454 RQLGCVGDVQWALFETKREAVARELERLRSTWVSPRILAEAEAERVLGKGIEREYALADL 513 Query: 301 LRRPNVTYDSLMTLAGADGQALAGPGVSDDPVREQIEIQIKYAGYIDRQAKEIGRQEHNE 360 LRRPNVTY++LM+L G DGQ L GPGV++ VREQ+EIQ+KYAGYIDRQA+E+ R +H E Sbjct: 514 LRRPNVTYETLMSLKGMDGQDLGGPGVAEPAVREQVEIQLKYAGYIDRQAREVERHDHYE 573 Query: 361 SLKLPADLDYMEVQALSIEVRQKLNKHKPETLGQASRISGVTPAALSLLLVHLKKGGLKN 420 +LKLPA+ DYM V+ LSIEVRQKL K +PETLGQASRISGVTPAA+SL+LVHLKKGG K Sbjct: 574 NLKLPAEFDYMAVKGLSIEVRQKLTKQRPETLGQASRISGVTPAAISLMLVHLKKGGFKE 633 Query: 421 VAEASANHNEAA 432 A N AA Sbjct: 634 FAALPPNTEAAA 645 >F1VTT1.1 F1VTT1_9BURK tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA Length = 644 Score = 739 bits (1909), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/431 (80%), Positives = 388/431 (90%) Query: 1 VLEEQPGDLDPIPVFSVMGNVGMHPQQMPCWITHTNTQTHDIIRAGLDRSPMYTGVIEGV 60 V++EQPGDLDPIPVFSVMG+V MHP+Q+PCWITHTN THDIIR GLDRSPMYTGVI G+ Sbjct: 214 VMQEQPGDLDPIPVFSVMGSVSMHPKQLPCWITHTNESTHDIIRGGLDRSPMYTGVIAGI 273 Query: 61 GPRYCPSIEDKIHRFAGKDSHQIYLEPEGLTTNEFYPNGISTSLPFDVQLALVRSMRGME 120 GPRYCPSIEDKIHRFA K SHQI+LEPEGLTTNE+YPNGISTSLPFDVQLALVRSM G+E Sbjct: 274 GPRYCPSIEDKIHRFADKSSHQIFLEPEGLTTNEYYPNGISTSLPFDVQLALVRSMVGLE 333 Query: 121 NAHILRPGYAIEYDYFDPRGLKSSLETRQIQGLFFAGQINGTTGYEEAAAQGMLAGLNAA 180 AHILRPGYAIEYDYFDPRGLK+SLET+ IQGLFFAGQINGTTGYEEAAAQGMLAGLNAA Sbjct: 334 QAHILRPGYAIEYDYFDPRGLKASLETKSIQGLFFAGQINGTTGYEEAAAQGMLAGLNAA 393 Query: 181 LQTQGRDSWTPRRDEAYLGVLVDDLITQGVLEPYRMFTSRAEYRLSLREDNADLRLTEIG 240 LQTQGRD+WTP RD+AYLGVLVDDL+TQGV EPYRMFTSRAEYRLSLREDNAD+RLTEIG Sbjct: 394 LQTQGRDAWTPGRDQAYLGVLVDDLVTQGVQEPYRMFTSRAEYRLSLREDNADMRLTEIG 453 Query: 241 RQLGCVGDAQWEMFEKKREAVAREMERLKSTWVNPRILAEAEAERVLGKAIDHEYSLFDL 300 R+LGCVGD QW FE+KREAVARE+ERL+STWV+PR+LAEAE+ERVLGKAI+ EYSL DL Sbjct: 454 RELGCVGDLQWAAFEQKREAVARELERLRSTWVSPRVLAEAESERVLGKAIEREYSLADL 513 Query: 301 LRRPNVTYDSLMTLAGADGQALAGPGVSDDPVREQIEIQIKYAGYIDRQAKEIGRQEHNE 360 LRRPNV+Y L+ L G +GQ+LAGPGV D VREQ+EIQ+KYAGYIDRQA+EI R E E Sbjct: 514 LRRPNVSYAGLLGLTGMEGQSLAGPGVVDLIVREQVEIQLKYAGYIDRQAREIERHEQFE 573 Query: 361 SLKLPADLDYMEVQALSIEVRQKLNKHKPETLGQASRISGVTPAALSLLLVHLKKGGLKN 420 LKLP + DY+ V ALS+EVRQKL+ H+PETLGQASRISGVTPAA+SLLLVHLKKGG Sbjct: 574 HLKLPVEFDYLAVSALSMEVRQKLHAHRPETLGQASRISGVTPAAMSLLLVHLKKGGYNE 633 Query: 421 VAEASANHNEA 431 + S+++ A Sbjct: 634 FLDTSSSNIAA 644 >E2SXC4.1 E2SXC4_9RALS tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG Length = 649 Score = 674 bits (1739), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/438 (73%), Positives = 373/438 (85%), Gaps = 8/438 (1%) Query: 1 VLEEQPGDLDPIPVFSVMGNVGMHPQQMPCWITHTNTQTHDIIRAGLDRSPMYTGVIEGV 60 VLEEQPGDLDP+PVFS MG MHP+Q+PCW+THTN +THDIIRAGLDRSPMYTGVIEGV Sbjct: 214 VLEEQPGDLDPVPVFSFMGRADMHPRQVPCWVTHTNERTHDIIRAGLDRSPMYTGVIEGV 273 Query: 61 GPRYCPSIEDKIHRFAGKDSHQIYLEPEGLTTNEFYPNGISTSLPFDVQLALVRSMRGME 120 GPRYCPSIEDKIHRFA KDSHQI+LEPEGLTTNEFYPNG+STSLPFDVQL L+ SMRG+E Sbjct: 274 GPRYCPSIEDKIHRFASKDSHQIFLEPEGLTTNEFYPNGVSTSLPFDVQLDLIHSMRGLE 333 Query: 121 NAHILRPGYAIEYDYFDPRGLKSSLETRQIQGLFFAGQINGTTGYEEAAAQGMLAGLNAA 180 NAHILRPGYAIEYDYFDPR LK+SLE++ I GLFFAGQINGTTGYEEAAAQG+LAG+NA Sbjct: 334 NAHILRPGYAIEYDYFDPRALKASLESKAISGLFFAGQINGTTGYEEAAAQGLLAGINAG 393 Query: 181 LQTQGRDSWTPRRDEAYLGVLVDDLITQGVLEPYRMFTSRAEYRLSLREDNADLRLTEIG 240 LQ QG+D+WTPRRD+AYLGVLVDDLIT+GV EPYRMFTSRAE+RLSLREDNAD+RLTE+G Sbjct: 394 LQVQGKDAWTPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLSLREDNADMRLTEVG 453 Query: 241 RQLGCVGDAQWEMFEKKREAVAREMERLKSTWVNPRILAEAEAERVLGKAIDHEYSLFDL 300 R LG V DA+W+ F +KR+AV+RE ERLKSTWVNP L +AE VLGK I+ EY+L DL Sbjct: 454 RALGVVDDARWDAFNRKRDAVSRETERLKSTWVNPATLPLEDAEPVLGKGIEREYALADL 513 Query: 301 LRRPNVTYDSLMTLAGADGQALAGPGVSDDP-----VREQIEIQIKYAGYIDRQAKEIGR 355 LRRPNVTY++LM + G AL P +++DP +REQ+EI IKY GYI RQA E+ R Sbjct: 514 LRRPNVTYETLMGMQGGK-YALESP-LAEDPLLAEQIREQVEIGIKYHGYIARQADEVER 571 Query: 356 QEHNESLKLPADLDYMEVQALSIEVRQKLNKHKPETLGQASRISGVTPAALSLLLVHLKK 415 NE+ +LPAD DY +V+ LSIEV+QKL KHKPET+GQASRISG+TPAA+SLLLVHLKK Sbjct: 572 LGANENTRLPADFDYSQVRGLSIEVQQKLAKHKPETIGQASRISGITPAAVSLLLVHLKK 631 Query: 416 GGLK-NVAEASANHNEAA 432 G L+ V + + EAA Sbjct: 632 GVLRGQVGPRATSEGEAA 649