BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0163.p (331 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IZ28.1 D8IZ28_HERSS Lipoate synthase protein 644 0.0 A6T345.1 LIPA_JANMA Lipoyl synthase EC=2.8.1.8 602 e-170 A4G9C6.1 LIPA_HERAR Lipoyl synthase EC=2.8.1.8 598 e-169 F1VY75.1 F1VY75_9BURK Lipoate synthase 581 e-164 Q12GR4.1 Q12GR4_POLSJ Lipoic acid synthetase 557 e-157 >D8IZ28.1 D8IZ28_HERSS Lipoate synthase protein Length = 330 Score = 644 bits (1660), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/330 (93%), Positives = 316/330 (95%), Gaps = 8/330 (2%) Query: 8 MTTEITPAAPTLRNAT--------DKQKGAGKTAWIPIKIMPAEKLKKPDWIRVKAGSPT 59 MTTE+TPAA + NAT DKQKGAGKTAWIPIK++ AEKLKKPDWIRVKAGSPT Sbjct: 1 MTTELTPAADSSSNATGSARANPLDKQKGAGKTAWIPIKVVAAEKLKKPDWIRVKAGSPT 60 Query: 60 TRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPD 119 TRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPD Sbjct: 61 TRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPD 120 Query: 120 PLDPNEPENLARTIAQLKLSYVVITSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILT 179 PLDPNEPENLA+TIAQL+LSYVVITSVDRDDLRDGGA HFAACIQRVRELSPETRIEILT Sbjct: 121 PLDPNEPENLAKTIAQLRLSYVVITSVDRDDLRDGGAAHFAACIQRVRELSPETRIEILT 180 Query: 180 PDFRGRMDRALEILKMAPPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTP 239 PDFRGRMDRALEILKMAPPDVMNHNLETAPRLYKEARPGSDYEYSL+LLKRFKDLHP+ P Sbjct: 181 PDFRGRMDRALEILKMAPPDVMNHNLETAPRLYKEARPGSDYEYSLSLLKRFKDLHPDVP 240 Query: 240 TKSGIMVGLGETDEEVLQVMRDMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEE 299 TKSGIMVGLGETDEEVLQVMRDMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEE Sbjct: 241 TKSGIMVGLGETDEEVLQVMRDMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEE 300 Query: 300 EAYKMGFVHAAVGAMVRSSYHADQQAHGLV 329 EAYKMGFVHAAVGAMVRSSYHADQQAHGLV Sbjct: 301 EAYKMGFVHAAVGAMVRSSYHADQQAHGLV 330 >A6T345.1 LIPA_JANMA Lipoyl synthase EC=2.8.1.8 Length = 331 Score = 602 bits (1552), Expect = e-170, Method: Compositional matrix adjust. Identities = 285/323 (88%), Positives = 297/323 (91%) Query: 8 MTTEITPAAPTLRNATDKQKGAGKTAWIPIKIMPAEKLKKPDWIRVKAGSPTTRFYEIKD 67 MTTE PA N ++KQKGA KT IPIK++P E+L KPDWIRVKA SP+TRFYEIKD Sbjct: 1 MTTETNPAVTPAYNPSEKQKGAAKTIRIPIKVIPMERLPKPDWIRVKAASPSTRFYEIKD 60 Query: 68 ILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPE 127 ILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLD NEPE Sbjct: 61 ILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPE 120 Query: 128 NLARTIAQLKLSYVVITSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMD 187 NLA+TIA L+L+YVVITSVDRDDLRDGGAGHFA CI+RVRELSP TRIEIL PDFRGRMD Sbjct: 121 NLAKTIAALRLNYVVITSVDRDDLRDGGAGHFAECIRRVRELSPNTRIEILVPDFRGRMD 180 Query: 188 RALEILKMAPPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVG 247 RALEIL APPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFK HPNTPTKSGIMVG Sbjct: 181 RALEILNAAPPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFKAQHPNTPTKSGIMVG 240 Query: 248 LGETDEEVLQVMRDMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFV 307 LGETDEEVLQVMRDMRAH+VDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGF Sbjct: 241 LGETDEEVLQVMRDMRAHDVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFA 300 Query: 308 HAAVGAMVRSSYHADQQAHGLVG 330 HAAVGAMVRSSYHADQQAHG+ Sbjct: 301 HAAVGAMVRSSYHADQQAHGVTS 323 >A4G9C6.1 LIPA_HERAR Lipoyl synthase EC=2.8.1.8 Length = 335 Score = 598 bits (1543), Expect = e-169, Method: Compositional matrix adjust. Identities = 285/326 (87%), Positives = 300/326 (92%), Gaps = 1/326 (0%) Query: 5 ITTMTTEITPAAPTLRNATDKQKGAGKTAWIPIKIMPAEKLKKPDWIRVKAGSPTTRFYE 64 I T TP+AP N ++KQKG+ KT IPIK++P E+L KPDWIRVKAGSP+TRFYE Sbjct: 3 IDTNPESSTPSAPAY-NPSEKQKGSAKTIRIPIKVVPMERLPKPDWIRVKAGSPSTRFYE 61 Query: 65 IKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPN 124 IKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLD N Sbjct: 62 IKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVN 121 Query: 125 EPENLARTIAQLKLSYVVITSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRG 184 EPENLA+TIA L+L+YVVITSVDRDDLRDGGAGHFA CI+RVRELSP TRIEIL PDFRG Sbjct: 122 EPENLAKTIAALRLNYVVITSVDRDDLRDGGAGHFAECIRRVRELSPNTRIEILVPDFRG 181 Query: 185 RMDRALEILKMAPPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGI 244 RMDRALEIL +APPDVMNHNLETAPRLYKEARPGSDY YSLNLLKRFK LHPNTPTKSGI Sbjct: 182 RMDRALEILNLAPPDVMNHNLETAPRLYKEARPGSDYAYSLNLLKRFKALHPNTPTKSGI 241 Query: 245 MVGLGETDEEVLQVMRDMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKM 304 MVGLGETDEEVLQVMRDMRAH+VDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKM Sbjct: 242 MVGLGETDEEVLQVMRDMRAHDVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKM 301 Query: 305 GFVHAAVGAMVRSSYHADQQAHGLVG 330 GF HAAVGAMVRSSYHADQQAHG+ Sbjct: 302 GFAHAAVGAMVRSSYHADQQAHGVTA 327 >F1VY75.1 F1VY75_9BURK Lipoate synthase Length = 330 Score = 581 bits (1497), Expect = e-164, Method: Compositional matrix adjust. Identities = 273/313 (87%), Positives = 290/313 (92%) Query: 15 AAPTLRNATDKQKGAGKTAWIPIKIMPAEKLKKPDWIRVKAGSPTTRFYEIKDILRANNL 74 A P N DKQKGAGKTA IPIKI+ AE+L KPDWIRVK+ P++RF+EIKDILRAN L Sbjct: 9 ATPLYYNPLDKQKGAGKTARIPIKIVQAERLPKPDWIRVKSALPSSRFFEIKDILRANKL 68 Query: 75 VTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLARTIA 134 VTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLD +EPENLA+TIA Sbjct: 69 VTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPENLAKTIA 128 Query: 135 QLKLSYVVITSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILK 194 L+L+YVVITSVDRDDLRDGGAGHFA CI+R+R LSP TRIEIL PDFRGRMDRALEIL Sbjct: 129 ALRLNYVVITSVDRDDLRDGGAGHFAECIRRIRALSPSTRIEILVPDFRGRMDRALEILN 188 Query: 195 MAPPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEE 254 +APPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEE Sbjct: 189 LAPPDVMNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEE 248 Query: 255 VLQVMRDMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAM 314 +LQVMRD+RAHNVDMLTIGQYL PSGDHLPVRRYVHPD FKM+E EAYKMGFVHAAVGAM Sbjct: 249 ILQVMRDLRAHNVDMLTIGQYLSPSGDHLPVRRYVHPDVFKMFEAEAYKMGFVHAAVGAM 308 Query: 315 VRSSYHADQQAHG 327 VRSSYHAD+QA G Sbjct: 309 VRSSYHADEQALG 321 >Q12GR4.1 Q12GR4_POLSJ Lipoic acid synthetase Length = 357 Score = 557 bits (1436), Expect = e-157, Method: Compositional matrix adjust. Identities = 260/307 (84%), Positives = 275/307 (89%) Query: 21 NATDKQKGAGKTAWIPIKIMPAEKLKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEE 80 N KQK A K + IP+K+ AE LKKPDWIRVKAGSPTTRFYEIK ILR N L TVCEE Sbjct: 38 NPLAKQKAAAKLSRIPVKVERAEVLKKPDWIRVKAGSPTTRFYEIKQILRENKLNTVCEE 97 Query: 81 ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLARTIAQLKLSY 140 ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLD NEPENLA+TIA L+L Y Sbjct: 98 ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPENLAKTIAALRLKY 157 Query: 141 VVITSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDV 200 VVITSVDRDDLRDGG+GHF CIQ++RELSP T IEIL PDFRGR DRALEILK APPDV Sbjct: 158 VVITSVDRDDLRDGGSGHFVECIQKIRELSPGTTIEILVPDFRGRDDRALEILKAAPPDV 217 Query: 201 MNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEEVLQVMR 260 MNHNLETAPRLYKEARPGSDY++SLNLLK+FK LHPN PTKSGIMVGLGETDEE+LQVMR Sbjct: 218 MNHNLETAPRLYKEARPGSDYQFSLNLLKKFKALHPNVPTKSGIMVGLGETDEEILQVMR 277 Query: 261 DMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYH 320 DMR HN+DMLTIGQYL PS HLPVRRYVHPDTF+M+EEEAYKMGF HAAVGAMVRSSYH Sbjct: 278 DMRTHNIDMLTIGQYLAPSTSHLPVRRYVHPDTFRMFEEEAYKMGFTHAAVGAMVRSSYH 337 Query: 321 ADQQAHG 327 ADQQAH Sbjct: 338 ADQQAHA 344