BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0057.p (397 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IUA8.1 D8IUA8_HERSS ATP-dependent DNA ligase protein EC=6.5.1.1 352 6e-95 C6XB41.1 C6XB41_METSD DNA ligase D 328 7e-88 Q5NYD8.1 Q5NYD8_AROAE ATP-dependent DNA ligase 286 3e-75 A9GRV1.1 A9GRV1_SORC5 High confidence in function and specificit... 271 1e-70 B5WRA1.1 B5WRA1_9BURK DNA ligase D 268 1e-69 >D8IUA8.1 D8IUA8_HERSS ATP-dependent DNA ligase protein EC=6.5.1.1 Length = 856 Score = 352 bits (903), Expect = 6e-95, Method: Compositional matrix adjust. Identities = 174/306 (56%), Positives = 220/306 (71%), Gaps = 12/306 (3%) Query: 1 VAEVKFAQWTRSGILRHGAFVALREDKKAEEIVREKAIATSRLEVKANAKGDAKEDAKHA 60 VAEV+FAQWT +GILRHG+FVALREDK +I RE + + A A Sbjct: 491 VAEVRFAQWTSAGILRHGSFVALREDKDPHQIRREGPQPLAEAKADAKAP---------- 540 Query: 61 PKSRAAKKPSANSSTNSSIESIEGMRLTHPDKLLFTDPDLSKRDLAEYYATVADWLLPHV 120 + A + +S S+ ++ G++LTHP K+LF D L+K +LA YY +VA W+LP + Sbjct: 541 --AAAPARSRGKASDLSAPVTVAGVKLTHPGKILFPDTGLTKLELARYYQSVAPWILPEL 598 Query: 121 QQRPMTMVRCPDGSVGKCFFQRHLGQGAPAAVEKIAVPEGKGSAEYMMVNDIAGLVATVQ 180 +QRP+++VRCPDG GK FFQ+H GQG P A++ I VPEG+G A+YMMVND+AGLVATVQ Sbjct: 599 RQRPLSLVRCPDGHAGKRFFQKHTGQGMPEAIDTIEVPEGEGHAQYMMVNDVAGLVATVQ 658 Query: 181 MGVLELHTWGAREGKLAQPDRIIFDLDPAPEVPWSKVVEGARLVHGLLEEIGLRSFVKTT 240 MGVLELH WGA L PDR++FDLDPA +VPW+ VVEGA+LV GLL+EIGL F+KT+ Sbjct: 659 MGVLELHAWGATGAALNHPDRVVFDLDPADDVPWATVVEGAQLVRGLLKEIGLDCFLKTS 718 Query: 241 GGKGLHVVVPIVPEQEWEVVKEWSRSVAEHMVQVLPDHFTAKMAKAARTGKIFIDYLRNA 300 GGKGLHV+VPI PE +W VVK+W+ A H+ + LPD F AKM+KAAR GKIFIDYLRNA Sbjct: 719 GGKGLHVMVPIRPEHDWAVVKDWAHRFAAHLARHLPDRFIAKMSKAARNGKIFIDYLRNA 778 Query: 301 ATAXKI 306 A + Sbjct: 779 PGATTV 784 >C6XB41.1 C6XB41_METSD DNA ligase D Length = 870 Score = 328 bits (842), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 160/306 (52%), Positives = 208/306 (67%), Gaps = 14/306 (4%) Query: 1 VAEVKFAQWTRSGILRHGAFVALREDKKAEEIVREKAIATSRLEVKANAKGDAKEDAKHA 60 VAEV+FAQWT S I+RH AFV LR+DK A EIV E AN EDA + Sbjct: 512 VAEVRFAQWTDSQIVRHAAFVGLRQDKPAREIVHE---------TPANPP-PPTEDAASS 561 Query: 61 PKSRAAKKPSANSSTNSSIESIEGMRLTHPDKLLFTDPDLSKRDLAEYYATVADWLLPHV 120 P+ ++P + S+ I G+RLTHP K+LF L+K+DLA YY +A W+LP + Sbjct: 562 PEQSGQQRPQ----RSGSVREIAGVRLTHPGKILFAQAGLTKQDLAHYYEEIAPWILPQL 617 Query: 121 QQRPMTMVRCPDGSVGKCFFQRHLGQGAPAAVEKIAVPEGKGSAEYMMVNDIAGLVATVQ 180 Q RP+++VRCP G+ +CFFQ+H P V +I VP S +YM+V+D+ L+ VQ Sbjct: 618 QGRPLSLVRCPTGAGQQCFFQKHAAMTIPDNVRRIQVPVSGFSDDYMVVDDLPALICLVQ 677 Query: 181 MGVLELHTWGAREGKLAQPDRIIFDLDPAPEVPWSKVVEGARLVHGLLEEIGLRSFVKTT 240 MGVLE+HTWG+RE L QPDRIIFDLDP +PW+ VVE A+LVH LL+ +GL SFVKTT Sbjct: 678 MGVLEIHTWGSREENLEQPDRIIFDLDPDEALPWTTVVESAQLVHALLQALGLESFVKTT 737 Query: 241 GGKGLHVVVPIVPEQEWEVVKEWSRSVAEHMVQVLPDHFTAKMAKAARTGKIFIDYLRNA 300 GGKG+HVV+PIVPE W+ +K +S+S A+++ + LPD FT+ M+K RTGK+FIDYLRNA Sbjct: 738 GGKGVHVVIPIVPEHSWQTIKAFSKSAAQYLEKQLPDRFTSNMSKEKRTGKVFIDYLRNA 797 Query: 301 ATAXKI 306 A I Sbjct: 798 PGATAI 803 >Q5NYD8.1 Q5NYD8_AROAE ATP-dependent DNA ligase Length = 742 Score = 286 bits (733), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 144/311 (46%), Positives = 199/311 (63%), Gaps = 7/311 (2%) Query: 1 VAEVKFAQWTRSGILRHGAFVALREDKKA----EEIVREKAIATSRLEVKANAKGDAKED 56 VAEV++A WTR +LR AF LREDK A +EI +A + + AK ++ Sbjct: 350 VAEVEYAGWTRDNLLRQAAFAGLREDKPANTVQDEIPLSPQVAAEMEQPPSQAKSPRRQK 409 Query: 57 AKHAPKSRAAKKPSANSSTNSSIESIEGMRLTHPDKLLFTDPDLSKRDLAEYYATVADWL 116 K + + KP A+S + + G+ +THP+++++ D L+K DLA YY +A WL Sbjct: 410 KKPSTERGGPAKPRASSESGEV--RVAGIVITHPERVVWPDAGLTKADLARYYEDIAPWL 467 Query: 117 LPHVQQRPMTMVRCPDGSVGKCFFQRHLGQGAPAAVEK-IAVPEGKGSAEYMMVNDIAGL 175 LPHV RP++++RCPDGS +CFFQRH+GQ P VE I K Y+ V + G+ Sbjct: 468 LPHVASRPLSLLRCPDGSEAECFFQRHMGQERPDGVESFIWEASSKDRRSYLYVTSLEGV 527 Query: 176 VATVQMGVLELHTWGAREGKLAQPDRIIFDLDPAPEVPWSKVVEGARLVHGLLEEIGLRS 235 + VQ GV+E HTWG+ + +PDRI DLDP PE+ W++VVE A+LV GLL E+G+ S Sbjct: 528 IGMVQRGVVEFHTWGSTMPRADRPDRITIDLDPGPELAWARVVEAAQLVRGLLGELGIAS 587 Query: 236 FVKTTGGKGLHVVVPIVPEQEWEVVKEWSRSVAEHMVQVLPDHFTAKMAKAARTGKIFID 295 FVKTTGGKGLHVVVP+ +W VK ++++VA H+ ++LPD FTA MA+ R G+IF+D Sbjct: 588 FVKTTGGKGLHVVVPLRRGPQWADVKRFAKAVATHLARILPDRFTANMARNQRAGRIFVD 647 Query: 296 YLRNAATAXKI 306 YLRN A A I Sbjct: 648 YLRNDAGASAI 658 >A9GRV1.1 A9GRV1_SORC5 High confidence in function and specificity EC=6.5.1.1 Length = 762 Score = 271 bits (694), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 136/311 (43%), Positives = 193/311 (62%), Gaps = 5/311 (1%) Query: 1 VAEVKFAQWTRSGILRHGAFVALREDKKAEEIVREKAIATSRL---EVKANAKGDAKEDA 57 VAEV+F++WTR G LRH +F LRED+ E + E+ + E A A Sbjct: 385 VAEVRFSEWTRDGRLRHPSFEGLREDRTPESVTVEREQPPPQSPPGEAAGAAPSRAAGAP 444 Query: 58 KHAPKSRAAKKPSANSSTNSSIESIE--GMRLTHPDKLLFTDPDLSKRDLAEYYATVADW 115 S A K + + E +E G+ LT+PDK+L+ ++K LA YY +A+ Sbjct: 445 PRRTASTAGGKSPRGGRSRGAAERVEVAGVPLTNPDKVLYPAQGITKLALARYYEQIAEH 504 Query: 116 LLPHVQQRPMTMVRCPDGSVGKCFFQRHLGQGAPAAVEKIAVPEGKGSAEYMMVNDIAGL 175 +L H ++R +T+VRCP G CFFQRH +G A++++AV E G Y+ + GL Sbjct: 505 MLTHAEKRLLTLVRCPAGQGKPCFFQRHEAEGLSQAIQRMAVEEDDGERTYLYIETTEGL 564 Query: 176 VATVQMGVLELHTWGAREGKLAQPDRIIFDLDPAPEVPWSKVVEGARLVHGLLEEIGLRS 235 V+ VQMG LE+H WG+R ++ QPDR++FDLDPAP++PW +V + AR LL +GLRS Sbjct: 565 VSLVQMGALEVHLWGSRVDRIEQPDRMVFDLDPAPDLPWERVADAARTARRLLSSLGLRS 624 Query: 236 FVKTTGGKGLHVVVPIVPEQEWEVVKEWSRSVAEHMVQVLPDHFTAKMAKAARTGKIFID 295 FVKTTGGKGLHVVVPI P + W+ VK ++++VA+ +V+ P +TA M+KA R GK+F+D Sbjct: 625 FVKTTGGKGLHVVVPIAPRRGWDEVKAFTKAVADALVRSDPARYTATMSKAKRAGKVFVD 684 Query: 296 YLRNAATAXKI 306 YLRN A + Sbjct: 685 YLRNGRGATAV 695 >B5WRA1.1 B5WRA1_9BURK DNA ligase D Length = 936 Score = 268 bits (684), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 132/316 (41%), Positives = 202/316 (63%), Gaps = 25/316 (7%) Query: 1 VAEVKFAQWTRSGILRHGAFVALREDKKAEEIVREKAIATSRLEVKANAKGDAKEDAKHA 60 VAE FA+WT GI+R +FV+LR+DK A +IVRE A + ++ + D K A Sbjct: 552 VAECNFAEWTSDGIVRQASFVSLRDDKPARQIVRETARQGADVQQQTETASDDDAPQKSA 611 Query: 61 PKSRAAKK---------PSANSSTNSSIE-SIEGMRLTHPDKLLFTDPDLSKRDLAEYYA 110 PK RAAK+ P+ +++T S + I G+R++HPD+++ SK +L +YY Sbjct: 612 PKKRAAKETARSATESAPARSTATRSRAQREIAGVRISHPDRVIDKSSGASKLELVQYYE 671 Query: 111 TVADWLLPHVQQRPMTMVRCPDGSVGKCFFQRHLGQGAPAAVEKIAVP-----EG--KGS 163 ++A+W+LPH+++RP+ +VR P+ G+ FFQRH +K+++P EG G Sbjct: 672 SIAEWMLPHLRERPVALVRAPEDIGGELFFQRH--------SQKLSIPHVTQHEGLDPGH 723 Query: 164 AEYMMVNDIAGLVATVQMGVLELHTWGAREGKLAQPDRIIFDLDPAPEVPWSKVVEGARL 223 + ++ +AGLV QMG +E HTW A + +PDR++FDLDP + W++++E A+L Sbjct: 724 PPLITIDTLAGLVGAAQMGTVEFHTWNALASNIEKPDRMVFDLDPDASLGWARMIEAAQL 783 Query: 224 VHGLLEEIGLRSFVKTTGGKGLHVVVPIVPEQEWEVVKEWSRSVAEHMVQVLPDHFTAKM 283 LL+E+GL+SF KT+GGKGLHVVVP+ + W+ VK++S+++A+HM + LP +F+AKM Sbjct: 784 TRSLLDELGLKSFCKTSGGKGLHVVVPLAKQAGWDEVKDFSQALAQHMAKTLPQYFSAKM 843 Query: 284 AKAARTGKIFIDYLRN 299 R KIFIDYLRN Sbjct: 844 GAQNRKKKIFIDYLRN 859