BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0032.p (549 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IWI1.1 D8IWI1_HERSS Glycogen debranching enzyme protein EC=3.2... 796 0.0 B2T9L5.1 B2T9L5_BURPP Glycogen debranching enzyme GlgX 712 0.0 B5WLU6.1 B5WLU6_9BURK Glycogen debranching enzyme GlgX 707 0.0 E8YQY5.1 E8YQY5_9BURK Glycogen debranching enzyme GlgX 706 0.0 B1FV36.1 B1FV36_9BURK Glycogen debranching enzyme GlgX 706 0.0 >D8IWI1.1 D8IWI1_HERSS Glycogen debranching enzyme protein EC=3.2.1.- Length = 753 Score = 796 bits (2056), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/568 (68%), Positives = 452/568 (79%), Gaps = 19/568 (3%) Query: 1 VIYEAHVRGISMMRDGVMPHERGTFAALGHPDFIAHLQKLGVTTLELMPVHAFLQENFLL 60 VIYEAHV+GIS +R+ ++PHERGTFAAL P FI HL +LGVTTLELMPVHAFLQ++FL+ Sbjct: 166 VIYEAHVKGISRLREDLLPHERGTFAALADPAFIDHLLRLGVTTLELMPVHAFLQDSFLV 225 Query: 61 QRDLRNYWGYSTLGFFAPEPAYLSTGDLNELRMAVRHFHAAGIEVILDVVYNHTCSGSEL 120 +R L NYWGYSTL +FAPEP YLS GD NELRMAVR H+AGIEVILDVVYNHT GSEL Sbjct: 226 ERGLHNYWGYSTLSYFAPEPGYLSMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSEL 285 Query: 121 GPTLSMRGLDNASYYRLLPGEERYYINDTGCGNTVNMSHPRVIQLVLDSLRYWAEAFDID 180 GPTLS RGLDNASY+RLLPG+ERY+INDTGCGNT+N+SHPRV+Q+VLDSLRYWA++F ID Sbjct: 286 GPTLSFRGLDNASYFRLLPGQERYHINDTGCGNTLNLSHPRVLQMVLDSLRYWAQSFHID 345 Query: 181 GFRFDLCSTLGREPNGFDPHSGFFDALMQDPVLSQLKLIAEPWDIGPGGYQLGNHPPRFA 240 GFRFDL STLGREP+GFDP SGFFDAL+QDPVLS+LKLI+EPWDIGPGGYQLGNHP RF Sbjct: 346 GFRFDLGSTLGREPHGFDPGSGFFDALLQDPVLSRLKLISEPWDIGPGGYQLGNHPARFG 405 Query: 241 EWNDKFRDAVRHFWAGGEGLRGEFAGRMLGSSDLFDHHHRKPWASVNFIASHDGFTLRDL 300 EWNDKFRD +R FW G E +RG FA RM+GS++LF+HH RKPWAS+NFIASHDGFTLRDL Sbjct: 406 EWNDKFRDDMRKFWKGDEAMRGAFAQRMMGSAELFEHHRRKPWASINFIASHDGFTLRDL 465 Query: 301 VSYETKHNEANGENGNDGHNENYSANWGVEGPTDNPEILQLRATVQRSMLTCVFLGQGTP 360 VSY KHNEANGEN NDGHN+N S NWG EG TD+ IL RA VQR+ML L QGTP Sbjct: 466 VSYNGKHNEANGENNNDGHNDNASYNWGAEGDTDDAAILAKRACVQRAMLASTLLAQGTP 525 Query: 361 MLLAGDEFHRTQRGNNNAYCQDNDISWVDWKNAASEEGQALTTYVGRLTAIRRDFPVLQG 420 MLLAGDEFHR+Q GNNNAYCQDN+ISWV+W A S EG AL+ +VGR+TAIRRDF VL+ Sbjct: 526 MLLAGDEFHRSQGGNNNAYCQDNEISWVNWDQARSPEGAALSAFVGRVTAIRRDFEVLRA 585 Query: 421 DRFLHGNREIEPGLKDVTWFDERATPISVEDWQNPVARALTLQCAGAGNDD--------P 472 FLHG++ + GL DV WFDE A P+S EDW NPVARA+TLQ AG + Sbjct: 586 SYFLHGSQMVADGLPDVAWFDEAARPVSSEDWNNPVARAITLQLAGKPQESHKQLSSRLH 645 Query: 473 DAG-NDQPGR-----PDIVLMFFNAGHEAISFTFPRPEDAHYLLI----DSNIPDGAVSR 522 DAG +D P PD++++F NAGHEAI F FPR E ++LL+ D+ IP G R Sbjct: 646 DAGQSDSPAEAAEATPDVLMIFVNAGHEAIDFHFPRTEGDYHLLLDTAQDTGIPTGEDVR 705 Query: 523 EACT-TASVEVAAHSVQVFANRPCREKS 549 +A+V+V AHS+QVFANRP S Sbjct: 706 LVLPGSATVQVPAHSLQVFANRPLHLAS 733 >B2T9L5.1 B2T9L5_BURPP Glycogen debranching enzyme GlgX Length = 739 Score = 712 bits (1838), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/547 (62%), Positives = 418/547 (76%), Gaps = 11/547 (2%) Query: 1 VIYEAHVRGISMMRDGVMPHERGTFAALGHPDFIAHLQKLGVTTLELMPVHAFLQENFLL 60 ++YE HVRG SM+R + HERGTFAAL P+FI HL KLGVT +EL+PVHAFL + FL+ Sbjct: 166 IVYETHVRGASMLRADLRQHERGTFAALSSPEFIEHLLKLGVTAVELLPVHAFLNDRFLV 225 Query: 61 QRDLRNYWGYSTLGFFAPEPAYLSTGDLNELRMAVRHFHAAGIEVILDVVYNHTCSGSEL 120 +R LRNYWGY+T FFAPEP+YLST L+E+R+AVR HAAGIEVILDVVYNHTC G+E+ Sbjct: 226 ERGLRNYWGYNTAAFFAPEPSYLSTHRLDEMRIAVRQLHAAGIEVILDVVYNHTCEGNEM 285 Query: 121 GPTLSMRGLDNASYYRLLPGEERYYINDTGCGNTVNMSHPRVIQLVLDSLRYWAEAFDID 180 GPT+S RGLDNASYYRL+PG+ER++INDTGCGNTVN+ HPRV+Q+V+DSLRYW+ AF+ID Sbjct: 286 GPTVSWRGLDNASYYRLIPGDERHHINDTGCGNTVNLPHPRVLQMVMDSLRYWSTAFNID 345 Query: 181 GFRFDLCSTLGREPNGFDPHSGFFDALMQDPVLSQLKLIAEPWDIGPGGYQLGNHPPRFA 240 GFRFDL TLGRE +GFDP SGFFDAL QDP+LSQ KLI+EPWDIGPGGYQLGNHPP F+ Sbjct: 346 GFRFDLGVTLGREQHGFDPGSGFFDALRQDPILSQRKLISEPWDIGPGGYQLGNHPPGFS 405 Query: 241 EWNDKFRDAVRHFWAGGEGLRGEFAGRMLGSSDLFDHHHRKPWASVNFIASHDGFTLRDL 300 EWND+FRD VR FW G GLR + A R+ GS+DLF+ RKPWASVNF+ SHDGFTL D+ Sbjct: 406 EWNDRFRDTVRRFWRGDAGLRPDLAARLTGSADLFNRRARKPWASVNFVTSHDGFTLADV 465 Query: 301 VSYETKHNEANGENGNDGHNENYSANWGVEGPTDNPEILQLRATVQRSMLTCVFLGQGTP 360 +YE KHNEAN E+ NDGHNEN S NWGVEGPTD+P IL R V RS++ + + GTP Sbjct: 466 TAYEQKHNEANREDNNDGHNENCSRNWGVEGPTDDPAILATRKRVARSLIATLLMALGTP 525 Query: 361 MLLAGDEFHRTQRGNNNAYCQDNDISWVDWKNAASEEGQALTTYVGRLTAIRRDFPVLQG 420 M+LAGDE RTQ GNNNAYCQDN+ISW+DW+ A S +GQ +T +V R+ A+R+ P+L+ Sbjct: 526 MVLAGDESLRTQNGNNNAYCQDNEISWIDWERANSPDGQQMTAFVARVIALRKQHPLLRE 585 Query: 421 DRFLHGNREIEPGLKDVTWFDERATPISVEDWQNPVARALTLQCAGAGNDDPDAGNDQPG 480 RFL G+RE+ PGL DV WFDE P+++E WQ+P RA TL+ AG G + G Sbjct: 586 TRFLFGDREVLPGLFDVGWFDEHGDPLTIEAWQDPEGRAFTLRRAGPGLN---------G 636 Query: 481 RPDIVLMFFNAGHEAISFTFPRPEDAHYLLIDSNIPDGAVSREACTTASVEVAAHSVQVF 540 +++LM NA E + FT P P ++L+D+ P+ A R A VEVAAHS+ + Sbjct: 637 ETEVLLMMLNANEETLRFTPPAPHLEWHVLLDTAEPESAPHRLAV--GEVEVAAHSLVML 694 Query: 541 ANRPCRE 547 A +P E Sbjct: 695 AAQPVGE 701 >B5WLU6.1 B5WLU6_9BURK Glycogen debranching enzyme GlgX Length = 737 Score = 707 bits (1824), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/547 (62%), Positives = 417/547 (76%), Gaps = 11/547 (2%) Query: 1 VIYEAHVRGISMMRDGVMPHERGTFAALGHPDFIAHLQKLGVTTLELMPVHAFLQENFLL 60 VIYE H+RG SM+R + HERGTFAALG P+FI HL KLGVT +EL+PVHAFL + FL+ Sbjct: 166 VIYETHLRGASMLRADLRQHERGTFAALGAPEFIEHLLKLGVTAVELLPVHAFLNDRFLV 225 Query: 61 QRDLRNYWGYSTLGFFAPEPAYLSTGDLNELRMAVRHFHAAGIEVILDVVYNHTCSGSEL 120 +R LRNYWGY+T FFAPEP YLST L+E+R+AVR HAAGIEVILDVVYNHTC +E+ Sbjct: 226 ERGLRNYWGYNTAAFFAPEPTYLSTHRLDEMRIAVRQLHAAGIEVILDVVYNHTCESNEM 285 Query: 121 GPTLSMRGLDNASYYRLLPGEERYYINDTGCGNTVNMSHPRVIQLVLDSLRYWAEAFDID 180 GPT+S RGLDNASYYRL+PG+ER+ INDTGCGNTVN+ HPRV+Q+V+DSLRYW+ AF+ID Sbjct: 286 GPTISWRGLDNASYYRLIPGDERHQINDTGCGNTVNLPHPRVMQMVMDSLRYWSTAFNID 345 Query: 181 GFRFDLCSTLGREPNGFDPHSGFFDALMQDPVLSQLKLIAEPWDIGPGGYQLGNHPPRFA 240 GFRFDL TLGRE +GFDP SGFFDAL QDPVLSQ KLI+EPWDIGPGGYQLGNHPP F+ Sbjct: 346 GFRFDLGVTLGRESHGFDPGSGFFDALRQDPVLSQRKLISEPWDIGPGGYQLGNHPPGFS 405 Query: 241 EWNDKFRDAVRHFWAGGEGLRGEFAGRMLGSSDLFDHHHRKPWASVNFIASHDGFTLRDL 300 EWND+FRD VR FW G GLR + A R+ GS+D+F+ HRKPWAS+NF+ SHDGFTL D+ Sbjct: 406 EWNDRFRDTVRRFWRGDAGLRPDLAARLTGSADVFNRRHRKPWASINFVTSHDGFTLADV 465 Query: 301 VSYETKHNEANGENGNDGHNENYSANWGVEGPTDNPEILQLRATVQRSMLTCVFLGQGTP 360 +YE KHNEAN E+ NDGHNEN S NWGVEG TD+P IL R V RS++ + + GTP Sbjct: 466 TAYEQKHNEANLEDNNDGHNENCSRNWGVEGATDDPAILATRRRVSRSLIATLMIALGTP 525 Query: 361 MLLAGDEFHRTQRGNNNAYCQDNDISWVDWKNAASEEGQALTTYVGRLTAIRRDFPVLQG 420 MLLAGDE RTQRGNNNAYCQDND+SW+DW A S+EG+ +T +VGR+ A+R+ P+L+ Sbjct: 526 MLLAGDEALRTQRGNNNAYCQDNDLSWIDWDLADSQEGRRMTEFVGRVIALRKRHPLLRE 585 Query: 421 DRFLHGNREIEPGLKDVTWFDERATPISVEDWQNPVARALTLQCAGAGNDDPDAGNDQPG 480 RFL G+REI PGL DV WFDE+ P+++E WQ+P RA TL+ AG G + G Sbjct: 586 TRFLFGDREILPGLFDVGWFDEQGDPLTIEAWQDPEGRAFTLRRAGPGLN---------G 636 Query: 481 RPDIVLMFFNAGHEAISFTFPRPEDAHYLLIDSNIPDGAVSREACTTASVEVAAHSVQVF 540 +++L+ NA E + F P P ++L+D+ P+ A R TA + VAAH + + Sbjct: 637 ETEVLLLMLNAHEEPLQFVPPAPHLEWHVLLDTADPERAPQR--LDTAELGVAAHGLVIL 694 Query: 541 ANRPCRE 547 A +P E Sbjct: 695 AAQPTGE 701 >E8YQY5.1 E8YQY5_9BURK Glycogen debranching enzyme GlgX Length = 739 Score = 706 bits (1823), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/547 (62%), Positives = 415/547 (75%), Gaps = 11/547 (2%) Query: 1 VIYEAHVRGISMMRDGVMPHERGTFAALGHPDFIAHLQKLGVTTLELMPVHAFLQENFLL 60 VIYE HVRG SM+R + HERGTFAAL P+FI HL KLGVT +EL+PVHAFL + FL+ Sbjct: 166 VIYETHVRGASMLRADLRQHERGTFAALASPEFIEHLLKLGVTAVELLPVHAFLNDRFLV 225 Query: 61 QRDLRNYWGYSTLGFFAPEPAYLSTGDLNELRMAVRHFHAAGIEVILDVVYNHTCSGSEL 120 +R LRNYWGY+T FFAPEP+YLST L+E+R+AVR HAAGIEVILDVVYNHTC G+E+ Sbjct: 226 ERGLRNYWGYNTAAFFAPEPSYLSTHRLDEMRIAVRQLHAAGIEVILDVVYNHTCEGNEM 285 Query: 121 GPTLSMRGLDNASYYRLLPGEERYYINDTGCGNTVNMSHPRVIQLVLDSLRYWAEAFDID 180 GPT+S RGLDNASYYRL+PG+ER++INDTGCGNTVN+ HPRV+Q+V+DSLRYW+ AF+ID Sbjct: 286 GPTVSWRGLDNASYYRLIPGDERHHINDTGCGNTVNLLHPRVLQMVMDSLRYWSTAFNID 345 Query: 181 GFRFDLCSTLGREPNGFDPHSGFFDALMQDPVLSQLKLIAEPWDIGPGGYQLGNHPPRFA 240 GFRFDL TLGREP+GFDP SGFFDA+ QDPVLSQ KLI+EPWDIGPGGYQLGNHPP F Sbjct: 346 GFRFDLGVTLGREPHGFDPGSGFFDAVRQDPVLSQRKLISEPWDIGPGGYQLGNHPPGFG 405 Query: 241 EWNDKFRDAVRHFWAGGEGLRGEFAGRMLGSSDLFDHHHRKPWASVNFIASHDGFTLRDL 300 EWND+FRD VR FW G GLR + A R+ GS+DLF+ RKPWAS+NF+ASHDGFTL D+ Sbjct: 406 EWNDRFRDTVRRFWRGDAGLRPDLAARLTGSADLFNRRFRKPWASINFVASHDGFTLADI 465 Query: 301 VSYETKHNEANGENGNDGHNENYSANWGVEGPTDNPEILQLRATVQRSMLTCVFLGQGTP 360 +YE KHNEAN E DGHNEN S+NWGVEGPTD+P IL R V RSM+ + + GTP Sbjct: 466 TAYEHKHNEANREGNRDGHNENCSSNWGVEGPTDDPAILDTRKRVARSMIATLLIALGTP 525 Query: 361 MLLAGDEFHRTQRGNNNAYCQDNDISWVDWKNAASEEGQALTTYVGRLTAIRRDFPVLQG 420 MLLAGDE RTQ GNNNAYCQDN+ISW+DW+ A S +G+ +T +V R+ A+R+ P+L+ Sbjct: 526 MLLAGDESLRTQDGNNNAYCQDNEISWIDWERAESPDGRQMTEFVSRVVALRKRHPLLRE 585 Query: 421 DRFLHGNREIEPGLKDVTWFDERATPISVEDWQNPVARALTLQCAGAGNDDPDAGNDQPG 480 RFL G+RE+ PGL DV WFDE P+++E W++P RA TL+ AG G G Sbjct: 586 TRFLFGDREVLPGLFDVGWFDENGDPLTIEAWEDPEGRAFTLRRAGPG---------LSG 636 Query: 481 RPDIVLMFFNAGHEAISFTFPRPEDAHYLLIDSNIPDGAVSREACTTASVEVAAHSVQVF 540 +++LM NA E + FT P P ++L+D+ A R A T +EVAAH + + Sbjct: 637 ETEVLLMMLNASAETLRFTPPAPHLEWHVLLDTADTHSAPHRLAAT--DLEVAAHGLVIL 694 Query: 541 ANRPCRE 547 A +P E Sbjct: 695 AAQPVGE 701 >B1FV36.1 B1FV36_9BURK Glycogen debranching enzyme GlgX Length = 735 Score = 706 bits (1821), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/547 (61%), Positives = 416/547 (76%), Gaps = 11/547 (2%) Query: 1 VIYEAHVRGISMMRDGVMPHERGTFAALGHPDFIAHLQKLGVTTLELMPVHAFLQENFLL 60 VIYE HVRG SM+R + HERGTFAAL P+FI HL KLGVT +EL+PVHAFL + FL+ Sbjct: 166 VIYETHVRGASMLRADLRQHERGTFAALSSPEFIEHLLKLGVTAVELLPVHAFLNDRFLV 225 Query: 61 QRDLRNYWGYSTLGFFAPEPAYLSTGDLNELRMAVRHFHAAGIEVILDVVYNHTCSGSEL 120 +R LRNYWGY+T FFAPEP+YLST L+E+R+AVR HAAGIEV LDVVYNHTC G+E+ Sbjct: 226 ERGLRNYWGYNTAAFFAPEPSYLSTHRLDEMRIAVRQLHAAGIEVFLDVVYNHTCEGNEM 285 Query: 121 GPTLSMRGLDNASYYRLLPGEERYYINDTGCGNTVNMSHPRVIQLVLDSLRYWAEAFDID 180 GPT+S RGLDNASYYRL+PG+ER++INDTGCGNTVN+ HPRV+Q+V+DSLRYW+ AF+ID Sbjct: 286 GPTVSWRGLDNASYYRLIPGDERHHINDTGCGNTVNLLHPRVLQMVMDSLRYWSTAFNID 345 Query: 181 GFRFDLCSTLGREPNGFDPHSGFFDALMQDPVLSQLKLIAEPWDIGPGGYQLGNHPPRFA 240 GFRFDL TLGREP+GFDP SGFFDAL QDPVLSQ KLI+EPWDIGPGGYQLGNHPP F Sbjct: 346 GFRFDLGVTLGREPHGFDPGSGFFDALRQDPVLSQRKLISEPWDIGPGGYQLGNHPPGFG 405 Query: 241 EWNDKFRDAVRHFWAGGEGLRGEFAGRMLGSSDLFDHHHRKPWASVNFIASHDGFTLRDL 300 EWND+FRD VR FW G GLR + A R+ GS+DLF+ RKPWAS+NF+ASHDGFTL D+ Sbjct: 406 EWNDRFRDTVRRFWRGDAGLRQDLAARLTGSADLFNRRFRKPWASINFVASHDGFTLADM 465 Query: 301 VSYETKHNEANGENGNDGHNENYSANWGVEGPTDNPEILQLRATVQRSMLTCVFLGQGTP 360 +YE KHNEAN E+ DGHNEN S+NWGVEGPTD+P IL+ R V RSM+ +F+ GTP Sbjct: 466 TAYEDKHNEANREDNRDGHNENCSSNWGVEGPTDDPAILETRKRVARSMMATLFVALGTP 525 Query: 361 MLLAGDEFHRTQRGNNNAYCQDNDISWVDWKNAASEEGQALTTYVGRLTAIRRDFPVLQG 420 M+LAGDE RTQ GNNNAYCQDN+ISW+DW+ A S +G+ +T +V R+ A+R+ P+L+ Sbjct: 526 MMLAGDESLRTQNGNNNAYCQDNEISWIDWERADSPDGRQMTDFVARVVALRKQHPLLRE 585 Query: 421 DRFLHGNREIEPGLKDVTWFDERATPISVEDWQNPVARALTLQCAGAGNDDPDAGNDQPG 480 RFL G+RE+ PGL D+ WFDE P+++E W++P RA TL+ AG G + G Sbjct: 586 TRFLFGDREVLPGLFDIGWFDENGEPLTIEAWEDPEGRAFTLRRAGPGLN---------G 636 Query: 481 RPDIVLMFFNAGHEAISFTFPRPEDAHYLLIDSNIPDGAVSREACTTASVEVAAHSVQVF 540 +++LM NA E + FT P P ++L+D+ GA R A +EVAA + + Sbjct: 637 ETEVLLMMLNASAETLRFTPPPPHLEWHVLLDTADTQGAPHRLAAM--DLEVAARGLVIL 694 Query: 541 ANRPCRE 547 A P E Sbjct: 695 AAHPVGE 701