BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0243.p (424 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J1N8.1 D8J1N8_HERSS Mandelate racemase/muconate lactonizing (D... 780 0.0 E2XQA0.1 E2XQA0_PSEFL Mandelate racemase/muconate lactonizing en... 756 0.0 Q4ZSW7.1 Q4ZSW7_PSEU2 Mandelate racemase/muconate lactonizing en... 743 0.0 C3K561.1 C3K561_PSEFS Putative glucarate dehydratase 742 0.0 Q881I2.1 Q881I2_PSESM Mandelate racemase/muconate lactonizing en... 741 0.0 >D8J1N8.1 D8J1N8_HERSS Mandelate racemase/muconate lactonizing (D-glucarate dehydratase) protein EC=5.1.2.2 Length = 424 Score = 780 bits (2014), Expect = 0.0, Method: Compositional matrix adjust. Identities = 372/424 (87%), Positives = 391/424 (92%) Query: 1 MKITRVTVTPIAFKDAPLLNASGIHEPYALRSIIEVETDNGHFGLGESYGDAAVLAVLDK 60 MKITRVTVTPIAFKD PLLNASGIHEPYALRSIIE+ETDNG+ GLGESYGDA L +LD Sbjct: 1 MKITRVTVTPIAFKDGPLLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDL 60 Query: 61 VKTQLIGLDPFNLNRLRAIVKAAVAGMSPANNAGAELAPGSHASKAVSNTYSAFEVALLD 120 VK +L+GLDPFNLN LRAIV+ VA M+PA+NAGAELAPGSHASKAVSN YSAFEVA LD Sbjct: 61 VKERLVGLDPFNLNGLRAIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLD 120 Query: 121 VQARYLNVPLVDLLGGAVRTDIPFSAYLFFKYAEHIDSPYKPDAWGEALSEEQIVAQARR 180 QARYLNVPLVDLLGGAVR +IPFSAYLFFKYA+HIDSPY PDAWGEALSEEQIVAQAR+ Sbjct: 121 AQARYLNVPLVDLLGGAVRKEIPFSAYLFFKYAQHIDSPYAPDAWGEALSEEQIVAQARK 180 Query: 181 MIDENGFQSIKLKAGALEPEHEVACIKALKKAFPDTPLRIDPNANWSLDTAVRMAELLGD 240 MI+ENGFQSIKLKAGAL PEHEVACIKAL+KAFPD PLRIDPN NWSL TA+RMAELLGD Sbjct: 181 MIEENGFQSIKLKAGALAPEHEVACIKALRKAFPDAPLRIDPNGNWSLQTAIRMAELLGD 240 Query: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL 300 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDE RRSVAL+SVQIVLADHHYWGGL Sbjct: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDELRRSVALDSVQIVLADHHYWGGL 300 Query: 301 RDTQMLALMCDTFGLGVSMHSNSHLGISLMAMSHVAAAVPNLSYACDTHYPWQEADEEVI 360 RDTQ LA MCD FGLGVSMHSNSHLGISLMAMSHVAAAV NLSYACDTHYPWQE DEEVI Sbjct: 301 RDTQQLAKMCDVFGLGVSMHSNSHLGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEVI 360 Query: 361 KGGKLPIRNGCVSITRAPGLGVELDRDQLAKLHDLYLGCGIRSRDDVRQMRKYKPDWKTV 420 +GGKLPIRNGCV IT APGLGVE+D+DQL KLH LYL CGIR RDDV QMRKY+PDWKTV Sbjct: 361 QGGKLPIRNGCVQITDAPGLGVEVDQDQLRKLHQLYLECGIRQRDDVGQMRKYQPDWKTV 420 Query: 421 KPRF 424 KPRF Sbjct: 421 KPRF 424 >E2XQA0.1 E2XQA0_PSEFL Mandelate racemase/muconate lactonizing enzyme EC=4.2.1.40 Length = 424 Score = 756 bits (1952), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/424 (83%), Positives = 389/424 (91%) Query: 1 MKITRVTVTPIAFKDAPLLNASGIHEPYALRSIIEVETDNGHFGLGESYGDAAVLAVLDK 60 MKI RVTVTPIAF+D PLLNASGIHEP+ALRSIIE+E+D G+ GLGESYGDA LA+ + Sbjct: 1 MKIVRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDTGYIGLGESYGDAPALAIQQQ 60 Query: 61 VKTQLIGLDPFNLNRLRAIVKAAVAGMSPANNAGAELAPGSHASKAVSNTYSAFEVALLD 120 V++QLIGLDPFNLN+LRAIV+A VA PA+ AGAELAPGSHASKAVSN YSAFEVA LD Sbjct: 61 VQSQLIGLDPFNLNQLRAIVQATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD 120 Query: 121 VQARYLNVPLVDLLGGAVRTDIPFSAYLFFKYAEHIDSPYKPDAWGEALSEEQIVAQARR 180 +QA LNVPLVDLLGGA+R IPFSAYLFFKYA+HIDSPYKPD+WGEAL+EEQIVAQARR Sbjct: 121 LQAHSLNVPLVDLLGGAIRDHIPFSAYLFFKYAQHIDSPYKPDSWGEALNEEQIVAQARR 180 Query: 181 MIDENGFQSIKLKAGALEPEHEVACIKALKKAFPDTPLRIDPNANWSLDTAVRMAELLGD 240 MI+ GF+SIKLKAGAL+PEHEVACIKALKKAFPD PLRIDPN NWSL+T++RMAELLGD Sbjct: 181 MIETYGFKSIKLKAGALQPEHEVACIKALKKAFPDVPLRIDPNGNWSLETSIRMAELLGD 240 Query: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL 300 DLQYYEDPTPGL+GMAELH RTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL Sbjct: 241 DLQYYEDPTPGLDGMAELHARTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL 300 Query: 301 RDTQMLALMCDTFGLGVSMHSNSHLGISLMAMSHVAAAVPNLSYACDTHYPWQEADEEVI 360 RDTQ+LA MC TFGLGVSMHSNSHLGISLMAM+HVAA+VPNL YACDTHYPWQE DEEVI Sbjct: 301 RDTQVLAKMCQTFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVI 360 Query: 361 KGGKLPIRNGCVSITRAPGLGVELDRDQLAKLHDLYLGCGIRSRDDVRQMRKYKPDWKTV 420 KGGKLPI +GCV ITRAPGLG+ELD DQL KLHD YL CGIR RDDV+QM++Y+PDWKTV Sbjct: 361 KGGKLPIIDGCVQITRAPGLGLELDHDQLGKLHDQYLSCGIRQRDDVKQMQRYQPDWKTV 420 Query: 421 KPRF 424 KPRF Sbjct: 421 KPRF 424 >Q4ZSW7.1 Q4ZSW7_PSEU2 Mandelate racemase/muconate lactonizing enzyme:Mandelate racemase/muconate lactonizing enzyme Length = 424 Score = 743 bits (1919), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/424 (82%), Positives = 387/424 (91%) Query: 1 MKITRVTVTPIAFKDAPLLNASGIHEPYALRSIIEVETDNGHFGLGESYGDAAVLAVLDK 60 MKI RVTVTPIAF+D PLLNASGIHEP+ALRSIIEVE+DNG+ GLGESYGDA LA+ + Sbjct: 1 MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEVESDNGYIGLGESYGDAPALAIQQQ 60 Query: 61 VKTQLIGLDPFNLNRLRAIVKAAVAGMSPANNAGAELAPGSHASKAVSNTYSAFEVALLD 120 V+TQLIGLDPFNLN+LR+IV+A VA PA+ AGAELAPGSHASKAVSN YSAFEVA LD Sbjct: 61 VQTQLIGLDPFNLNQLRSIVQATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD 120 Query: 121 VQARYLNVPLVDLLGGAVRTDIPFSAYLFFKYAEHIDSPYKPDAWGEALSEEQIVAQARR 180 +QAR +N+PLVDLLGGA+R IPFSAYLFFKYA+H D+PY PD+WGEALSEEQIVAQARR Sbjct: 121 LQARSMNLPLVDLLGGAIRDQIPFSAYLFFKYAQHADAPYAPDSWGEALSEEQIVAQARR 180 Query: 181 MIDENGFQSIKLKAGALEPEHEVACIKALKKAFPDTPLRIDPNANWSLDTAVRMAELLGD 240 MID GF+SIKLKAGAL+PEHEV+CIKALKKAFP PLRIDPN NWSL+T++RMAELLGD Sbjct: 181 MIDAYGFKSIKLKAGALQPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD 240 Query: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL 300 DLQYYEDPTPGL+GM+ELH+RTGLPLATNMVVTDFDEFRRSVAL+SVQIVLADHHYWGGL Sbjct: 241 DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALDSVQIVLADHHYWGGL 300 Query: 301 RDTQMLALMCDTFGLGVSMHSNSHLGISLMAMSHVAAAVPNLSYACDTHYPWQEADEEVI 360 RDTQ LA MC+TFGLGVSMHSNSHLGISLMAM+HVAA+VPNL YACDTHYPWQE DEEVI Sbjct: 301 RDTQALAKMCETFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVI 360 Query: 361 KGGKLPIRNGCVSITRAPGLGVELDRDQLAKLHDLYLGCGIRSRDDVRQMRKYKPDWKTV 420 KGGKLPI +GCVSITRAPG+G+ELD DQL KL+D Y CGIR RDDV+QM+KY PDWK V Sbjct: 361 KGGKLPIVDGCVSITRAPGMGLELDYDQLGKLNDQYHSCGIRQRDDVKQMQKYNPDWKAV 420 Query: 421 KPRF 424 KPR+ Sbjct: 421 KPRY 424 >C3K561.1 C3K561_PSEFS Putative glucarate dehydratase Length = 424 Score = 742 bits (1915), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/424 (83%), Positives = 382/424 (90%) Query: 1 MKITRVTVTPIAFKDAPLLNASGIHEPYALRSIIEVETDNGHFGLGESYGDAAVLAVLDK 60 MKI RVTVTPIAF+D PLLNASGIHE YALRSIIEVE+D G+ GLGESYGDA LA+ Sbjct: 1 MKIVRVTVTPIAFRDPPLLNASGIHEAYALRSIIEVESDTGYIGLGESYGDAPALAIKQL 60 Query: 61 VKTQLIGLDPFNLNRLRAIVKAAVAGMSPANNAGAELAPGSHASKAVSNTYSAFEVALLD 120 V+ QLIGLDPFNLN LRAIV+A VA PA+ +GAELAPGSHASKAVSN YSAFEVALLD Sbjct: 61 VQPQLIGLDPFNLNGLRAIVQATVAAHKPASLSGAELAPGSHASKAVSNAYSAFEVALLD 120 Query: 121 VQARYLNVPLVDLLGGAVRTDIPFSAYLFFKYAEHIDSPYKPDAWGEALSEEQIVAQARR 180 +QA LNVPLVDLLGGA+R IPFSAYLFFKYAEHIDSPYKPD+WGEAL+EEQIVAQARR Sbjct: 121 LQAHALNVPLVDLLGGAIRDRIPFSAYLFFKYAEHIDSPYKPDSWGEALNEEQIVAQARR 180 Query: 181 MIDENGFQSIKLKAGALEPEHEVACIKALKKAFPDTPLRIDPNANWSLDTAVRMAELLGD 240 MI+ GF+SIKLKAGALEPEHEV CIKALKKAFP PLRIDPN NWSL T++RMAELLGD Sbjct: 181 MIEAYGFKSIKLKAGALEPEHEVRCIKALKKAFPGVPLRIDPNGNWSLQTSIRMAELLGD 240 Query: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL 300 DLQYYEDPTPGLEGMAELH RTGLPLATNMVVTDFDEFRRSVA +SVQIVLADHHYWGGL Sbjct: 241 DLQYYEDPTPGLEGMAELHTRTGLPLATNMVVTDFDEFRRSVAQDSVQIVLADHHYWGGL 300 Query: 301 RDTQMLALMCDTFGLGVSMHSNSHLGISLMAMSHVAAAVPNLSYACDTHYPWQEADEEVI 360 RDTQ LA MC TFGLGVSMHSNSHLGISLMAM+HVAA+VPNL YACDTHYPWQE DEEVI Sbjct: 301 RDTQALAKMCQTFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVI 360 Query: 361 KGGKLPIRNGCVSITRAPGLGVELDRDQLAKLHDLYLGCGIRSRDDVRQMRKYKPDWKTV 420 KGGK+PI +GCV++TRAPGLG+ELD DQL KLHD YL CGIR RDDV+QM++Y+PDWKTV Sbjct: 361 KGGKVPIVDGCVTLTRAPGLGLELDHDQLGKLHDQYLSCGIRQRDDVKQMQRYQPDWKTV 420 Query: 421 KPRF 424 KPRF Sbjct: 421 KPRF 424 >Q881I2.1 Q881I2_PSESM Mandelate racemase/muconate lactonizing enzyme family protein Length = 424 Score = 741 bits (1914), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/424 (82%), Positives = 387/424 (91%) Query: 1 MKITRVTVTPIAFKDAPLLNASGIHEPYALRSIIEVETDNGHFGLGESYGDAAVLAVLDK 60 MKI RVTVTPIAF+D PLLNASGIHEP+ALRSIIE+E+DNG+ GLGESYGDA LA+ + Sbjct: 1 MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDNGYIGLGESYGDAPALAIQQQ 60 Query: 61 VKTQLIGLDPFNLNRLRAIVKAAVAGMSPANNAGAELAPGSHASKAVSNTYSAFEVALLD 120 V+ QLIG+DPFNLN+LR+IV+A VA PA+ AGAELAPGSHASKAVSN YSAFEVA LD Sbjct: 61 VQNQLIGIDPFNLNQLRSIVQATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD 120 Query: 121 VQARYLNVPLVDLLGGAVRTDIPFSAYLFFKYAEHIDSPYKPDAWGEALSEEQIVAQARR 180 +QAR +N+PLVDLLGGA+R IPFSAYLFFKYA+HID+PY PD WGEAL+EEQIVAQARR Sbjct: 121 LQARSMNLPLVDLLGGAIRERIPFSAYLFFKYAQHIDTPYPPDNWGEALNEEQIVAQARR 180 Query: 181 MIDENGFQSIKLKAGALEPEHEVACIKALKKAFPDTPLRIDPNANWSLDTAVRMAELLGD 240 MI+ GF+SIKLKAGAL+PEHEV+CIKALKKAFP PLRIDPN NWSL+T++RMA+LLGD Sbjct: 181 MIEAYGFKSIKLKAGALDPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAQLLGD 240 Query: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL 300 DLQYYEDPTPGL+GM+ELH+RTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL Sbjct: 241 DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL 300 Query: 301 RDTQMLALMCDTFGLGVSMHSNSHLGISLMAMSHVAAAVPNLSYACDTHYPWQEADEEVI 360 RDTQ LA MCDTFGLGVSMHSNSHLGISLMAM+HVAA+VPNL YACDTHYPWQEADEEVI Sbjct: 301 RDTQALAKMCDTFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEADEEVI 360 Query: 361 KGGKLPIRNGCVSITRAPGLGVELDRDQLAKLHDLYLGCGIRSRDDVRQMRKYKPDWKTV 420 KGGKLPI +GCVSITRAPGLG+ELD DQL KL+D Y CGIR RDDVRQM+KY PDWK V Sbjct: 361 KGGKLPIVDGCVSITRAPGLGLELDYDQLGKLNDQYHSCGIRQRDDVRQMQKYTPDWKAV 420 Query: 421 KPRF 424 KP++ Sbjct: 421 KPQY 424