BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0223.p (296 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IT64.1 D8IT64_HERSS Methylglyoxal synthase protein EC=4.2.3.3 508 e-142 A6SWW2.1 A6SWW2_JANMA Uncharacterized conserved protein 467 e-130 A4SZC9.1 A4SZC9_POLSQ Putative uncharacterized protein 397 e-109 Q2L230.1 Q2L230_BORA1 Putative methylglyoxal synthase EC=4.2.3.3 326 3e-87 E5U678.1 E5U678_ALCXX Putative uncharacterized protein 322 4e-86 >D8IT64.1 D8IT64_HERSS Methylglyoxal synthase protein EC=4.2.3.3 Length = 295 Score = 508 bits (1309), Expect = e-142, Method: Compositional matrix adjust. Identities = 246/293 (83%), Positives = 264/293 (90%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARIEALQPELVVVGRTLDAIGHLGLL 60 M+S + RYRIGL ANRAHQDAP+SALVQLL GSR RIE+LQPEL+VVGRTLDAIG + LL Sbjct: 1 MSSIAPRYRIGLIANRAHQDAPDSALVQLLTGSRHRIESLQPELIVVGRTLDAIGQMNLL 60 Query: 61 PGYPHIERFPYGRQGGLMKLVSRVVDSDPARMLDAIIYLIDPVDPSSTFPEAVALKRQCV 120 PGY H+ RFPYGR GGLMKLVSR VD DP R LDA+IYLIDPVDPSSTFPEAVALKRQCV Sbjct: 61 PGYEHLVRFPYGRHGGLMKLVSRTVDEDPERTLDAVIYLIDPVDPSSTFPEAVALKRQCV 120 Query: 121 IHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALAE 180 IHGKPFLSTLAGAQEWFELEA+A GAAPD + D FRL EGIALIAHD+MK+RML LAE Sbjct: 121 IHGKPFLSTLAGAQEWFELEALANGAAPDASLDDRFRLGQEGIALIAHDAMKSRMLELAE 180 Query: 181 RHFDVLDSFGFRCATGTTGGLLNELAVKIKGEAGRNWVKPFLSGPLGGDAQIAELILEHE 240 +HF VLD F FRCATGTTGGLLN+LA+KIKGEAGR+WVKPFLSGPLGGDAQIAELILE + Sbjct: 181 KHFAVLDQFAFRCATGTTGGLLNQLAMKIKGEAGRHWVKPFLSGPLGGDAQIAELILEQQ 240 Query: 241 CRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQR 293 CRRVLFLEDPHVARQHEADIQLLERAARTVT YASCMSD KWGDRWL+LI QR Sbjct: 241 CRRVLFLEDPHVARQHEADIQLLERAARTVTAYASCMSDVKWGDRWLTLIGQR 293 >A6SWW2.1 A6SWW2_JANMA Uncharacterized conserved protein Length = 315 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 227/296 (76%), Positives = 260/296 (87%), Gaps = 3/296 (1%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARIEA-LQPELVVVGRTLDAIGHLGL 59 MT++ ++RIGLA +R HQDAPNS L +LL GSR IE LQP+L+VVGRTLDA+ HLGL Sbjct: 14 MTTTPHQFRIGLATSRTHQDAPNSVLARLLNGSRIAIEQHLQPQLIVVGRTLDAMDHLGL 73 Query: 60 LPGYPHIERFPYGRQGGLMKLVSRVVDSDPARMLDAIIYLIDPVDPSSTFPEAVALKRQC 119 L YPHIERFPYGR GGLMKLVSRVVD+DPAR LDA++YL+DPVDPSS FPEAVALKRQC Sbjct: 74 LKNYPHIERFPYGRHGGLMKLVSRVVDTDPARRLDAVMYLMDPVDPSSIFPEAVALKRQC 133 Query: 120 VIHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALA 179 VIHGKPFLSTLAGA+EW ELE+IA GAAP+P D +F L +EGIA+IAHD+MK RML +A Sbjct: 134 VIHGKPFLSTLAGAREWLELESIAIGAAPNPELDETFDLKNEGIAMIAHDAMKGRMLEIA 193 Query: 180 ERHFDVLDSFGFRCATGTTGGLLNELAVKIKG-EAGRNWVKPFLSGPLGGDAQIAELILE 238 ERHFD+LD FGFRCATGTTG LLN+LA KIKG EAGRNWVKP+LSGP+GGDAQIAELIL+ Sbjct: 194 ERHFDLLDQFGFRCATGTTGSLLNKLAQKIKGEEAGRNWVKPYLSGPMGGDAQIAELILD 253 Query: 239 H-ECRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQR 293 +CRRVLFLEDPHVARQHEADIQLLERAARTVTDYA C+S+E G+RWL L+++R Sbjct: 254 KDQCRRVLFLEDPHVARQHEADIQLLERAARTVTDYALCISNENCGERWLDLLQKR 309 >A4SZC9.1 A4SZC9_POLSQ Putative uncharacterized protein Length = 296 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 198/295 (67%), Positives = 228/295 (77%), Gaps = 2/295 (0%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARI-EALQPELVVVGRTLDAIGHLGL 59 M + YRIGL ANRAHQD+ ++ L+QLL GS+A I E L+P VVGRT+DA+ L Sbjct: 1 MVNEVPNYRIGLVANRAHQDSEDATLIQLLNGSKASITETLKPHFSVVGRTMDAMKIYDL 60 Query: 60 LPGYPHIERFPYGRQGGLMKLVSRVVDSDPARMLDAIIYLIDPVDPSSTFPEAVALKRQC 119 LP Y IER PYG GGLM+LVSRVVD DP + L+AIIYL+DPVDPSS FPEAVALKRQC Sbjct: 61 LPDYADIERLPYGHDGGLMRLVSRVVDPDPNKALNAIIYLMDPVDPSSAFPEAVALKRQC 120 Query: 120 VIHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALA 179 VIHGKPFLSTLAGA+EW ELE++ D F ++GIALIAHD+MK ML LA Sbjct: 121 VIHGKPFLSTLAGAREWIELESMNLTGKKTKDLDPYFAYENQGIALIAHDAMKPEMLRLA 180 Query: 180 ERHFDVLDSFGFRCATGTTGGLLNELAVKIKG-EAGRNWVKPFLSGPLGGDAQIAELILE 238 E+HFD+LD F R ATGTTGGLLNELA +IKG + G WV P LSGP+GGDAQIAEL+L+ Sbjct: 181 EKHFDLLDRFKVRYATGTTGGLLNELAKQIKGSKDGEGWVTPCLSGPMGGDAQIAELVLD 240 Query: 239 HECRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQR 293 +C R+LFLEDPHVARQHEADIQLLERAAR VTD+ASC SD K DRWLSL+ QR Sbjct: 241 RKCGRILFLEDPHVARQHEADIQLLERAARVVTDFASCNSDNKSADRWLSLLSQR 295 >Q2L230.1 Q2L230_BORA1 Putative methylglyoxal synthase EC=4.2.3.3 Length = 298 Score = 326 bits (835), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 167/297 (56%), Positives = 206/297 (69%), Gaps = 3/297 (1%) Query: 1 MTSSSTRYRIGLAANRAHQDAPNSALVQLLQGSRARIEALQPELVVVGRTLDAIGHLGLL 60 MT S R+ GLAANR H + P++AL L+ S A I + EL VGRT DAI G+L Sbjct: 1 MTVPSLRF--GLAANRLHHETPDAALFNWLRASGASIREMGIELHTVGRTHDAIVREGML 58 Query: 61 PGYPHIERFPYGRQGGLMKLVSRVVDS-DPARMLDAIIYLIDPVDPSSTFPEAVALKRQC 119 GY + R+PYGR+GGLMKLV+RV D D IYLIDPVDPSS FPEA+ALKRQC Sbjct: 59 QGYRGLIRYPYGREGGLMKLVARVTQGRDGQAPFDGAIYLIDPVDPSSIFPEALALKRQC 118 Query: 120 VIHGKPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALA 179 + HG+PF+STLAGA EW E+E + G APDPA + FR + +A+IAHD++K M+A A Sbjct: 119 ITHGRPFISTLAGAIEWIEIERVLAGLAPDPALNRLFRFEGQTLAMIAHDALKDEMMAFA 178 Query: 180 ERHFDVLDSFGFRCATGTTGGLLNELAVKIKGEAGRNWVKPFLSGPLGGDAQIAELILEH 239 + +FDVL F R ATGTTGG LNELA + WV +LSGPLGGDAQIAEL+LEH Sbjct: 179 DANFDVLSQFARRVATGTTGGRLNELAWSRGWPKDQPWVTRYLSGPLGGDAQIAELVLEH 238 Query: 240 ECRRVLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQRQVR 296 +C+RV+F EDPHVARQHEADIQLLERA R VT+ A+C + RW +++ Q + Sbjct: 239 QCQRVIFFEDPHVARQHEADIQLLERAVRVVTESATCATSPSVARRWADAVRRHQAQ 295 >E5U678.1 E5U678_ALCXX Putative uncharacterized protein Length = 296 Score = 322 bits (825), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 165/293 (56%), Positives = 200/293 (68%), Gaps = 1/293 (0%) Query: 5 STRYRIGLAANRAHQDAPNSALVQLLQGSRARIEALQPELVVVGRTLDAIGHLGLLPGYP 64 + + R GLAANR H + P++AL L+ + I L +L VGRT DAI G+L GYP Sbjct: 3 AAQLRFGLAANRLHHETPDAALFTWLRACSSTIRELGVQLHTVGRTHDAIVREGMLQGYP 62 Query: 65 HIERFPYGRQGGLMKLVSRVVDS-DPARMLDAIIYLIDPVDPSSTFPEAVALKRQCVIHG 123 + R+PYGR+GGLMKLV+RV + D D IYLIDPVDPSS FPEA+ALKRQC+ HG Sbjct: 63 GLIRYPYGREGGLMKLVARVTEGRDGQAPFDGAIYLIDPVDPSSIFPEALALKRQCITHG 122 Query: 124 KPFLSTLAGAQEWFELEAIAGGAAPDPARDGSFRLADEGIALIAHDSMKARMLALAERHF 183 +PF+STLAGA EW E+E + G APD A F A + +ALIAHD++K M+A A HF Sbjct: 123 RPFISTLAGAIEWMEVERVHAGLAPDAAASRLFNYAGQTLALIAHDALKDEMVAFAAEHF 182 Query: 184 DVLDSFGFRCATGTTGGLLNELAVKIKGEAGRNWVKPFLSGPLGGDAQIAELILEHECRR 243 DVL F R ATGTTGG LNELA WV +LSGPLGGDAQIAEL+LEH+C+R Sbjct: 183 DVLSQFARRVATGTTGGRLNELAWSRGWPKDTPWVHRYLSGPLGGDAQIAELVLEHQCQR 242 Query: 244 VLFLEDPHVARQHEADIQLLERAARTVTDYASCMSDEKWGDRWLSLIKQRQVR 296 V+F EDPHVARQHEADIQLLERA R VT+ A+C + RW ++ R R Sbjct: 243 VIFFEDPHVARQHEADIQLLERAVRVVTESATCATSPSVARRWAKAVELRAGR 295