BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0042.p (345 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8NJA8.1 D8NJA8_RALSO Nitrilase EC=3.5.5.1 521 e-146 F2K7Y4.1 F2K7Y4_PSEBR Putative nitrilase 489 e-136 C5ANM8.1 C5ANM8_BURGB Putative nitrilase 473 e-131 B6JH36.1 B6JH36_OLICO Aliphatic nitrilase 456 e-126 Q6N9I6.1 Q6N9I6_RHOPA Putative nitrilase EC=3.5.5.7 445 e-123 >D8NJA8.1 D8NJA8_RALSO Nitrilase EC=3.5.5.1 Length = 338 Score = 521 bits (1343), Expect = e-146, Method: Compositional matrix adjust. Identities = 250/340 (73%), Positives = 288/340 (84%), Gaps = 2/340 (0%) Query: 1 MNLPTVKVAAMHVAPVYMDSAATLQKALSLIGEAAANGAELIVFPESFIPGFPVWAALWA 60 M+LPTVKVAA+H APV+++ ATLQKAL+ I EAA NGAELI FPESFIPGFPVWAALW+ Sbjct: 1 MSLPTVKVAAVHAAPVFLNREATLQKALAFINEAARNGAELIAFPESFIPGFPVWAALWS 60 Query: 61 PIYNHEWFKKMVANSLLIDGPEMTELRATAARHRVFVSMGFSEATPVSVGCIWNSNVLIG 120 PI NH+WF+KM ANS+L+DGPE+ L A AAR VFVSMGFSE+T VSVGC+WN+NVLIG Sbjct: 61 PIDNHDWFRKMAANSVLVDGPEIAALAAEAARLGVFVSMGFSESTRVSVGCLWNANVLIG 120 Query: 121 DDGSLLNHHRKLVPTFYEKMVWAPGDGAGLKVAATRIGKIGGLICGENTNPLARYSLIAQ 180 DDG+LLNHHRKLVPTFYEKMVWAPGDGAGL+VA TRIGKIGGLICGENTNPLARY+LIAQ Sbjct: 121 DDGTLLNHHRKLVPTFYEKMVWAPGDGAGLRVADTRIGKIGGLICGENTNPLARYALIAQ 180 Query: 181 GEQIHISSWPPIWPTKKPASGENFDNVAANRIRASAHSFEAKAYGILCGSCLDRSAFDML 240 GEQIH+SSWP IWPT+KP SGENFDN+AANRIRASAHSFEAKA+GILC L R D L Sbjct: 181 GEQIHLSSWPAIWPTRKPVSGENFDNLAANRIRASAHSFEAKAFGILCAGYLGRDMVDQL 240 Query: 241 TAEDKSVAGLLERTPKAATQFVTPTGTQIGDSLQADEGIAYASFDLSACIEPKQFHDVVG 300 + D A +LE+TP+AATQF++PTG+ +GD LQ +EGIAYA FDL+ C+EPKQFHDVVG Sbjct: 241 SI-DPRAAEVLEQTPRAATQFISPTGSGLGDVLQDEEGIAYARFDLNGCVEPKQFHDVVG 299 Query: 301 YYNRFDVFDLAVNRKRIQPITWEGAEAPLADEPGATAVSA 340 YYNRFDVFDL +NR+R+ P+TW +A G T V A Sbjct: 300 YYNRFDVFDLKINRRRLAPVTW-ADDAQTQRAAGQTVVDA 338 >F2K7Y4.1 F2K7Y4_PSEBR Putative nitrilase Length = 341 Score = 489 bits (1258), Expect = e-136, Method: Compositional matrix adjust. Identities = 227/323 (70%), Positives = 267/323 (82%), Gaps = 2/323 (0%) Query: 1 MNLPTVKVAAMHVAPVYMDSAATLQKALSLIGEAAANGAELIVFPESFIPGFPVWAALWA 60 M+LPTVKVAA H A VYM+ AA + KA S I EAAANGAEL+VFPESFIPGFPVWAALWA Sbjct: 1 MSLPTVKVAAAHAASVYMNKAACVDKACSFIAEAAANGAELVVFPESFIPGFPVWAALWA 60 Query: 61 PIYNHEWFKKMVANSLLIDGPEMTELRATAARHRVFVSMGFSEATPVSVGCIWNSNVLIG 120 PIYNH+WFK MV NS++IDGPE+T +R+ A RH + VS+GFSEATP SVGCIWNSNVLI Sbjct: 61 PIYNHDWFKLMVENSVMIDGPEITAIRSAARRHGILVSLGFSEATPASVGCIWNSNVLIS 120 Query: 121 DDGSLLNHHRKLVPTFYEKMVWAPGDGAGLKVAATRIGKIGGLICGENTNPLARYSLIAQ 180 + G++LNHHRKLVPTFYEKMVWAPGDG GL+V+ TRIGKIG LICGENTNPLA+YSL AQ Sbjct: 121 EQGTVLNHHRKLVPTFYEKMVWAPGDGHGLRVSDTRIGKIGTLICGENTNPLAKYSLAAQ 180 Query: 181 GEQIHISSWPPIWPTKKPASGENFDNVAANRIRASAHSFEAKAYGILCGSCLDRSAFDML 240 GEQIHIS+WP IWPT+KP G+NFDN+AANRIRA A FEAK +GIL +D S DML Sbjct: 181 GEQIHISAWPAIWPTRKPG-GQNFDNLAANRIRAGAQCFEAKVFGILNAGFMDDSMLDML 239 Query: 241 TA-EDKSVAGLLERTPKAATQFVTPTGTQIGDSLQADEGIAYASFDLSACIEPKQFHDVV 299 +A D+ +L +P+AATQF+ P G IGD LQ +EG+AYA+FDL+ C+EPKQFHD+ Sbjct: 240 SASSDEQAIEVLRNSPRAATQFLDPAGASIGDLLQNEEGLAYATFDLNECVEPKQFHDIA 299 Query: 300 GYYNRFDVFDLAVNRKRIQPITW 322 GYYNRFDVFDL ++R+RI P T+ Sbjct: 300 GYYNRFDVFDLTIDRRRITPATF 322 >C5ANM8.1 C5ANM8_BURGB Putative nitrilase Length = 338 Score = 473 bits (1216), Expect = e-131, Method: Compositional matrix adjust. Identities = 220/321 (68%), Positives = 264/321 (82%) Query: 6 VKVAAMHVAPVYMDSAATLQKALSLIGEAAANGAELIVFPESFIPGFPVWAALWAPIYNH 65 +KVAA HVAPV++D AAT+ KA++LI EA + GAELIVFPESFIP FPVWA+LWAPI NH Sbjct: 1 MKVAAAHVAPVFLDRAATVAKAVNLIREARSAGAELIVFPESFIPAFPVWASLWAPIDNH 60 Query: 66 EWFKKMVANSLLIDGPEMTELRATAARHRVFVSMGFSEATPVSVGCIWNSNVLIGDDGSL 125 + F++ VA S+ DGPE+ E+RA A R +FVS+GFSE + SVGC+WNSN+LI D G++ Sbjct: 61 QLFERFVAASIYADGPEIAEIRAEAERSGIFVSLGFSERSRASVGCLWNSNLLIDDRGAV 120 Query: 126 LNHHRKLVPTFYEKMVWAPGDGAGLKVAATRIGKIGGLICGENTNPLARYSLIAQGEQIH 185 LNHHRKLVPTF+EK+VWAPGDG GL+VA TRIG++GGLICGENTNPLARY+LIAQGEQIH Sbjct: 121 LNHHRKLVPTFFEKLVWAPGDGHGLRVADTRIGRVGGLICGENTNPLARYTLIAQGEQIH 180 Query: 186 ISSWPPIWPTKKPASGENFDNVAANRIRASAHSFEAKAYGILCGSCLDRSAFDMLTAEDK 245 ISSWP +WPT++P +G N+DNVAANRIRA AHSFEAKA+GI+C +D D L D Sbjct: 181 ISSWPALWPTRRPDTGGNYDNVAANRIRAGAHSFEAKAFGIMCAGYMDTPMRDALLKVDP 240 Query: 246 SVAGLLERTPKAATQFVTPTGTQIGDSLQADEGIAYASFDLSACIEPKQFHDVVGYYNRF 305 SVA +L+ +P+AAT FV PTGT IGD+L ADEGIAYA DL+ C+EPKQFHDVVGYYNR+ Sbjct: 241 SVAEVLDGSPRAATMFVDPTGTPIGDTLSADEGIAYAEMDLAKCVEPKQFHDVVGYYNRY 300 Query: 306 DVFDLAVNRKRIQPITWEGAE 326 DVF L VNR R +PI W AE Sbjct: 301 DVFSLNVNRTRQEPIRWTDAE 321 >B6JH36.1 B6JH36_OLICO Aliphatic nitrilase Length = 354 Score = 456 bits (1172), Expect = e-126, Method: Compositional matrix adjust. Identities = 220/334 (65%), Positives = 263/334 (78%), Gaps = 4/334 (1%) Query: 6 VKVAAMHVAPVYMDSAATLQKALSLIGEAAANGAELIVFPESFIPGFPVWAALWAPIYNH 65 VKVAA+H APV++D AAT KA+S++ EAA GAEL+ FPE++IP FPVWAALWAPI NH Sbjct: 5 VKVAAVHAAPVFLDRAATTAKAISILREAANAGAELVAFPETYIPAFPVWAALWAPIDNH 64 Query: 66 EWFKKMVANSLLIDGPEMTELRATAARHRVFVSMGFSEATPVSVGCIWNSNVLIGDDGSL 125 + F M S+ + GPE+ LR A V VSMG SE++ SVG IWNSNVLIG +G + Sbjct: 65 DLFTLMAEQSVSLHGPEIAALRREARALGVIVSMGISESSNASVGAIWNSNVLIGSNGDI 124 Query: 126 LNHHRKLVPTFYEKMVWAPGDGAGLKVAATRIGKIGGLICGENTNPLARYSLIAQGEQIH 185 LNHHRKLVPTFYEK+VWA GDGAGLKV++T IG+IG LICGENTNPLARY+L+AQ EQIH Sbjct: 125 LNHHRKLVPTFYEKLVWAAGDGAGLKVSSTPIGRIGNLICGENTNPLARYTLMAQAEQIH 184 Query: 186 ISSWPPIWPTKKPASGENFDNVAANRIRASAHSFEAKAYGILCGSCLDRSAFDMLTAEDK 245 ISSWPP+WPT++P++G NF+NVAANRIRASAHSFEAKAYGI+ +D + D L A D Sbjct: 185 ISSWPPMWPTRRPSTGGNFNNVAANRIRASAHSFEAKAYGIVTAGYMDAAMRDFLIARDP 244 Query: 246 SVAGLLERTPKAATQFVTPTGTQIGDSLQADEGIAYASFDLSACIEPKQFHDVVGYYNRF 305 SV +++RT +AA+ FV PTG +GD LQ EGIAYA FDL+ CIEPKQFHDVVGYYNRF Sbjct: 245 SVVEVIDRTQRAASFFVDPTGEAVGDLLQDQEGIAYAEFDLTRCIEPKQFHDVVGYYNRF 304 Query: 306 DVFDLAVNRKRIQPITW-EGAEAPLA---DEPGA 335 DVFDL VNRKR++P T+ + AE P A DE GA Sbjct: 305 DVFDLTVNRKRLKPATFLDIAETPAASETDESGA 338 >Q6N9I6.1 Q6N9I6_RHOPA Putative nitrilase EC=3.5.5.7 Length = 349 Score = 445 bits (1144), Expect = e-123, Method: Compositional matrix adjust. Identities = 205/320 (64%), Positives = 254/320 (79%) Query: 3 LPTVKVAAMHVAPVYMDSAATLQKALSLIGEAAANGAELIVFPESFIPGFPVWAALWAPI 62 LP K AA+H APV++D AAT +KA+SLI EA A GAEL+ FPE++IP FPVWAALWAPI Sbjct: 2 LPHFKAAAIHAAPVFLDKAATTKKAISLIREAVAAGAELVAFPETYIPAFPVWAALWAPI 61 Query: 63 YNHEWFKKMVANSLLIDGPEMTELRATAARHRVFVSMGFSEATPVSVGCIWNSNVLIGDD 122 NH+ F +M S+LIDGPE+ +R A R V VS+G SE +P SVG IWNSN+LIG+D Sbjct: 62 DNHDLFVRMADQSVLIDGPEVKAIRDEARRLGVVVSIGISEKSPASVGGIWNSNLLIGED 121 Query: 123 GSLLNHHRKLVPTFYEKMVWAPGDGAGLKVAATRIGKIGGLICGENTNPLARYSLIAQGE 182 G +LNHHRKLVPTFYEK++W+ GDGAGL+V TR+GKIG LICGENTNPLARY+L+AQGE Sbjct: 122 GEILNHHRKLVPTFYEKLIWSVGDGAGLRVVDTRLGKIGQLICGENTNPLARYALMAQGE 181 Query: 183 QIHISSWPPIWPTKKPASGENFDNVAANRIRASAHSFEAKAYGILCGSCLDRSAFDMLTA 242 Q HISSWPP+WPT++PA G N+ AA RIRASAH FEAK +G++ LD++A DML A Sbjct: 182 QFHISSWPPVWPTRRPAEGGNYHIAAATRIRASAHCFEAKVFGLVTSGVLDKAARDMLVA 241 Query: 243 EDKSVAGLLERTPKAATQFVTPTGTQIGDSLQADEGIAYASFDLSACIEPKQFHDVVGYY 302 D S A +L+ TP+AAT F+ PTG QIG++L DEGI YA DL+ C+EPKQFHDVVGYY Sbjct: 242 RDPSAAAVLDGTPRAATFFLDPTGEQIGEALCEDEGILYADIDLNRCVEPKQFHDVVGYY 301 Query: 303 NRFDVFDLAVNRKRIQPITW 322 NRFDVF ++++R+R+ P T+ Sbjct: 302 NRFDVFAVSISRRRLSPATF 321