BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0701.p (286 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IUP1.1 D8IUP1_HERSS Bifunctional: biotin operon repressor/biot... 302 4e-80 A4G9B4.1 A4G9B4_HERAR Putative bifunctional protein birA EC=6.3.... 253 2e-65 A6T333.1 A6T333_JANMA Bifunctional biotin operon repressor /biot... 253 2e-65 F1VYQ5.1 F1VYQ5_9BURK Biotin-protein ligase 237 1e-60 E1T5V6.1 E1T5V6_BURSG Biotin/acetyl-CoA-carboxylase ligase 199 5e-49 >D8IUP1.1 D8IUP1_HERSS Bifunctional: biotin operon repressor/biotin-[acetyl-CoA-carboxylase] synthetase protein EC=6.3.4.15 Length = 257 Score = 302 bits (773), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 157/264 (59%), Positives = 197/264 (74%), Gaps = 9/264 (3%) Query: 23 LIASSSNDNAKTVAVEVVAETGSTNADLLARVTAVDGARLVAPVLLVAESQTAGRGRAGR 82 L+A S + + + VE VA TGSTN DL+ RVT+ L P+LLVA+ QTAGRGRAGR Sbjct: 2 LLAGSDSQGQQRIDVEAVACTGSTNVDLMQRVTS-----LTRPLLLVADRQTAGRGRAGR 56 Query: 83 PWQTSKTSALTFSLAWKFGRPLHALIGLPLAVGVALAEALGIFGIDVELKWPNDVLRDGA 142 PW+ + +LTFSLAW+F RP+HAL+GLPLAVGVALAEAL +FG+ +LKWPNDVLR+GA Sbjct: 57 PWRAAPEDSLTFSLAWRFERPMHALLGLPLAVGVALAEALEVFGVRPQLKWPNDVLREGA 116 Query: 143 KLAGILIETAQDKGAGAAGIAGTWTVIGIGVNLTMPPDLQQRIGRAVADVSELLTHDRER 202 KLAGILIETA D G A TW VIGIG+NL +P L+Q + RA+A V EL RE Sbjct: 117 KLAGILIETASDGRRGDA----TWAVIGIGLNLGVPQGLEQELQRAIAAVPELRKPPRET 172 Query: 203 LLAILLSALSDALADFDARGFTHFVARWNRLHAYTGRAVRILDDGRMLHEGVAVGVDEQG 262 +LAI+ SAL++ L +F+ RGF FV RW HAY G+AV+I+D+GR+LHEG AVGVD G Sbjct: 173 VLAIIASALAEVLTEFEQRGFGAFVRRWEAFHAYAGQAVKIVDNGRLLHEGRAVGVDMMG 232 Query: 263 CFLMDTASGRVAVTAGDVSLRLRE 286 FL+DT +G+VAV GD+SLRL++ Sbjct: 233 RFLLDTEAGQVAVMVGDLSLRLQD 256 >A4G9B4.1 A4G9B4_HERAR Putative bifunctional protein birA EC=6.3.4.15 Length = 263 Score = 253 bits (647), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 146/279 (52%), Positives = 179/279 (64%), Gaps = 19/279 (6%) Query: 8 ETSLATTISAQRIAALIASSSNDNAKTVAVEVVAETGSTNADLLARVTAVDGARLVAPVL 67 E SL T ++AAL S + + + +V ETGSTNADLLA T + G P L Sbjct: 3 EPSLPTV---PQLAALCGS----EVQNMTLRIVTETGSTNADLLAETTQLQG-----PTL 50 Query: 68 LVAESQTAGRGRAGRPWQTSKTSALTFSLAWKFGRPLHALIGLPLAVGVALAEALGIFGI 127 L A QTAG+GRAGR W ++ + LTFSLAWKF P+ AL GL LAVGV + E L FG+ Sbjct: 51 LWAGVQTAGKGRAGRRWHSAADATLTFSLAWKFALPVQALAGLSLAVGVTVVETLISFGV 110 Query: 128 DVELKWPNDVLRDGAKLAGILIETAQDKGAGAAGIAGTWTVIGIGVNLTMPPDLQQRIGR 187 D +LKWPNDVLR+G KLAGILIETA DK W +IG+G+NL +L +IGR Sbjct: 111 DAQLKWPNDVLRNGNKLAGILIETAMDK----TDRQSIWAIIGVGINLADARNLSAQIGR 166 Query: 188 AVADVSELLTHDRERLLAILLSALSDALADFDARGFTHFVARWNRLHAYTGRAVRILDDG 247 VAD S L +R RL+A LL A S AL F+ +GF F WN L AY G+AV ILD Sbjct: 167 TVADASGL---ERVRLMAALLDAFSKALPLFEQQGFKAFAPAWNALDAYAGQAVNILDQE 223 Query: 248 RMLHEGVAVGVDEQGCFLMDTASGRVAVTAGDVSLRLRE 286 ++L G A GVDE G L+DTA GR+A++AGDVSLR+RE Sbjct: 224 KILQHGDAAGVDEAGRLLLDTAQGRIAISAGDVSLRIRE 262 >A6T333.1 A6T333_JANMA Bifunctional biotin operon repressor /biotin--[acetyl-CoA-carboxylase] synthetase EC=6.3.4.15 Length = 307 Score = 253 bits (646), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 150/271 (55%), Positives = 181/271 (66%), Gaps = 14/271 (5%) Query: 16 SAQRIAALIASSSNDNAKTVAVEVVAETGSTNADLLARVTAVDGARLVAPVLLVAESQTA 75 SAQR+A L ++ A+ +A+E+VAETGSTNADLLAR L P LL A+ QTA Sbjct: 50 SAQRLAGLCTNA----AQAIALEIVAETGSTNADLLARAP-----HLQQPTLLWAQVQTA 100 Query: 76 GRGRAGRPWQTSKTSALTFSLAWKFGRPLHALIGLPLAVGVALAEALGIFGIDVELKWPN 135 G+GRAGR W T +ALTFSLAWKF PL L+GLPLAVGV +AEAL FG+D LKWPN Sbjct: 101 GKGRAGRAWHTPAGAALTFSLAWKFNLPLQGLLGLPLAVGVVIAEALRTFGVDARLKWPN 160 Query: 136 DVLRDGAKLAGILIETAQDKGAGAAGIAGTWTVIGIGVNLTMPPDLQQRIGRAVADVSEL 195 DVL+D KLAGILIET DK +G +W VIG+G+N+ DL + GRAV +E Sbjct: 161 DVLKDDGKLAGILIETVADK----SGQPASWVVIGVGINIARASDLSAQTGRAVVAATE- 215 Query: 196 LTHDRERLLAILLSALSDALADFDARGFTHFVARWNRLHAYTGRAVRILDDGRMLHEGVA 255 L + ER +A LL + AL F+ GF FV WN L AY G+AV ILD G++LH G A Sbjct: 216 LAAEPERAMAALLDGFAQALPRFEQEGFAGFVDAWNALDAYAGQAVNILDQGQVLHSGHA 275 Query: 256 VGVDEQGCFLMDTASGRVAVTAGDVSLRLRE 286 GVD G L+DTA GR+ V AGDVSLR+RE Sbjct: 276 AGVDAGGRLLLDTAQGRITVVAGDVSLRIRE 306 >F1VYQ5.1 F1VYQ5_9BURK Biotin-protein ligase Length = 264 Score = 237 bits (605), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 146/262 (55%), Positives = 176/262 (67%), Gaps = 9/262 (3%) Query: 22 ALIASSSNDNAKTVAVEVVAETGSTNADLLARVTAVDGARLVAPVLLVAESQTAGRGRAG 81 A IA++ + +E VAETGSTNADLLAR+ + G PV LVA+ QTAGRGRAG Sbjct: 8 ARIAAALRTAVPGLTIEAVAETGSTNADLLARLDQLHG-----PVFLVADQQTAGRGRAG 62 Query: 82 RPWQTSKTSALTFSLAWKFGRPLHALIGLPLAVGVALAEALGIFGIDVELKWPNDVLRDG 141 R W + + ++LTFSLAW PL AL+GLPLAVGVALA+ L GI V+LKWPND+L DG Sbjct: 63 RTWHSERGASLTFSLAWPVRVPLQALVGLPLAVGVALADVLAGHGIAVQLKWPNDLLADG 122 Query: 142 AKLAGILIETAQDKGAGAAGIAGTWTVIGIGVNLTMPPDLQQRIGRAVADVSELLTHDRE 201 +KLAGILIETA D+ AGA W VIGIG+NLT L RIG+ VA + + DR+ Sbjct: 123 SKLAGILIETAVDRQAGAR----LWAVIGIGINLTDNAALAARIGQPVAALPDAGAIDRD 178 Query: 202 RLLAILLSALSDALADFDARGFTHFVARWNRLHAYTGRAVRILDDGRMLHEGVAVGVDEQ 261 LLA LL L L +F+ GF F RWN LH + G+ V I+D G+ + EGVAVGVD Q Sbjct: 179 LLLADLLGHLCLTLQEFEEYGFAVFAERWNALHTHRGQPVSIIDRGQTVCEGVAVGVDAQ 238 Query: 262 GCFLMDTASGRVAVTAGDVSLR 283 G L+DTA GR V AGDVSLR Sbjct: 239 GRLLLDTAQGRRTVVAGDVSLR 260 >E1T5V6.1 E1T5V6_BURSG Biotin/acetyl-CoA-carboxylase ligase Length = 305 Score = 199 bits (505), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 131/289 (45%), Positives = 161/289 (55%), Gaps = 29/289 (10%) Query: 15 ISAQRIAALIASSSNDNAKTVAVEVVAETGSTNADLLARVTAVD--GARLVAPVLLVAES 72 I +R L + ++D +E+V ETGSTNADL+ARV A+ L P++ VA Sbjct: 22 IDRERAVTLFGAHAHD----WPIEIVEETGSTNADLMARVKALPRRAGALPRPIVRVAYL 77 Query: 73 QTAGRGRAGRPWQTSKTSALTFSLAWKFGRPLHALIGLPLAVGVALAEALGIFGI----D 128 QTAGRGR GRPW +AL FS+A RPL L GL LAVGVAL + L + Sbjct: 78 QTAGRGRRGRPWYAEPGNALLFSVACVLPRPLEGLAGLSLAVGVALVDGLRSLPVAGPGQ 137 Query: 129 VELKWPNDVLRDGAKLAGILIETAQDKGAGAAGIAGTWTVIGIGVNLTMPPDLQQRIGRA 188 + LKWPNDVL +G KLAGILIETA +A VIGIG N+ +L +IG Sbjct: 138 IALKWPNDVLLEGDKLAGILIETAWSTEHASA------VVIGIGTNVKGADELAAKIGAL 191 Query: 189 VADVSE-------------LLTHDRERLLAILLSALSDALADFDARGFTHFVARWNRLHA 235 AD L + LA L+AL AL F A GF F ARWN +HA Sbjct: 192 TADAPPQARGTAPTALQRALPNANLTDTLAAELNALEPALQRFGAEGFAPFQARWNAMHA 251 Query: 236 YTGRAVRILDDGRMLHEGVAVGVDEQGCFLMDTASGRVAVTAGDVSLRL 284 Y GR V +L+ G + GVA GVDE+G L+DTA+GR +V GDVSLRL Sbjct: 252 YAGREVVLLEQGEEVTRGVAAGVDERGQLLLDTAAGRQSVATGDVSLRL 300