BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0132.p (278 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IXA6.1 D8IXA6_HERSS Carboxymethylenebutenolidase (Dienelactone... 445 e-123 A4G1W1.1 A4G1W1_HERAR Putative carboxymethylenebutenolidase EC=3... 409 e-112 A6SUR7.1 A6SUR7_JANMA Carboxymethylenebutenolidase EC=3.1.1.45 404 e-111 F1W331.1 F1W331_9BURK Putative carboxymethylenebutenolidase (Die... 370 e-100 Q1LK27.1 Q1LK27_RALME Putative carboxymethylenebutenolidase (Die... 349 3e-94 >D8IXA6.1 D8IXA6_HERSS Carboxymethylenebutenolidase (Dienelactone hydrolase) protein EC=3.1.1.45 Length = 294 Score = 445 bits (1145), Expect = e-123, Method: Compositional matrix adjust. Identities = 216/293 (73%), Positives = 242/293 (82%), Gaps = 16/293 (5%) Query: 1 MNDLKNDVDSLAGTTGLLDAGNRRTFI----------------XQTMIKTDTVGLTAGEV 44 MNDL+ND +SL G + L +RR+F+ QTMIKTDT GLTAGEV Sbjct: 1 MNDLRNDFNSLLGQSTLSGNADRRSFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 60 Query: 45 SVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 104 S+ VNGQ+VPAYRAQPE KT LPVI+V+SEIFGVHEHIADIARRFAKLGYLAIAPDFFVR Sbjct: 61 SIEVNGQRVPAYRAQPERKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 120 Query: 105 QGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVW 164 QGNP AY +IAEL K+IISKVPD QV GD+DAYV WAG++GG+ ++GINGFCWGGRIVW Sbjct: 121 QGNPMAYTSIAELQKDIISKVPDAQVMGDIDAYVKWAGEHGGDIRRLGINGFCWGGRIVW 180 Query: 165 LYSARNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQ 224 LYSA NPK+KAGAAWYGR+VGEKS L PQ PIDIAPTLKTPV+GLYGGKD GIPV+TVEQ Sbjct: 181 LYSAHNPKVKAGAAWYGRLVGEKSELFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ 240 Query: 225 MKEALAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 MKEAL K GNKSEF V+ NSGHAFNADYRPSYVEADAK+ W + V +FK HGV Sbjct: 241 MKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGV 293 >A4G1W1.1 A4G1W1_HERAR Putative carboxymethylenebutenolidase EC=3.1.1.45 Length = 294 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 200/293 (68%), Positives = 229/293 (78%), Gaps = 16/293 (5%) Query: 1 MNDLKNDVDSLAGTTGLLDAGNRRTFI----------------XQTMIKTDTVGLTAGEV 44 M +L+ D DSL T L D NRR FI Q +IKTD GLTAGE+ Sbjct: 1 MKNLQEDFDSLVAKTPLSDGVNRRGFIKTALGTGFAAAVLPVSAQHVIKTDDAGLTAGEI 60 Query: 45 SVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 104 +V VNGQ VP YRAQP+GKT+LPVILVISEIFGVHE+IAD+ARRFAKLGYLA+AP+ FVR Sbjct: 61 TVNVNGQAVPVYRAQPKGKTDLPVILVISEIFGVHEYIADVARRFAKLGYLALAPELFVR 120 Query: 105 QGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVW 164 QG+P +Y TIAEL KEIISKVPD QV DLDA VAWA NGGN K+GI GFCWGGRI W Sbjct: 121 QGDPGSYGTIAELQKEIISKVPDAQVMTDLDAVVAWAKANGGNTGKLGITGFCWGGRITW 180 Query: 165 LYSARNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQ 224 LY+A NP+IKAG AWYGR+VG+ + L P++PIDIA LK PVLGLYG +DGGIPV+TV Q Sbjct: 181 LYAAHNPQIKAGVAWYGRLVGDSTPLTPKNPIDIAAKLKAPVLGLYGAQDGGIPVSTVVQ 240 Query: 225 MKEALAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 MKEALAK +KSEF+VF NSGHAF+ADYR SYVEADAKEGW+ A+ WFK+HGV Sbjct: 241 MKEALAKGSSKSEFIVFKNSGHAFHADYRASYVEADAKEGWQHAITWFKTHGV 293 >A6SUR7.1 A6SUR7_JANMA Carboxymethylenebutenolidase EC=3.1.1.45 Length = 294 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/293 (65%), Positives = 227/293 (77%), Gaps = 16/293 (5%) Query: 1 MNDLKNDVDSLAGTTGLLDAGNRRTFIXQTM----------------IKTDTVGLTAGEV 44 M++LK D DSL T + D +RR F+ + + TD+ GLT GE+ Sbjct: 1 MDNLKEDFDSLVAKTPISDGVDRRGFLKTALGSGFAAAVLPIAAHSAVTTDSKGLTVGEI 60 Query: 45 SVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVR 104 +V VNGQ VP YRAQP GKTNLPV+LVISEIFGVHE+IAD+ARRFAKLGYLA+AP+ FVR Sbjct: 61 TVNVNGQAVPVYRAQPAGKTNLPVVLVISEIFGVHEYIADVARRFAKLGYLALAPELFVR 120 Query: 105 QGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVW 164 QG+P +Y TIAEL KEIISKVPD QV DLDA VAWA NGGN DK+GI GFCWGGR+ W Sbjct: 121 QGDPGSYGTIAELQKEIISKVPDAQVMTDLDAVVAWAKANGGNTDKLGITGFCWGGRVTW 180 Query: 165 LYSARNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQ 224 LYSA NPKIKAG AWYGR+VG + L P +P+DIA LKTPVLGLYG +DGGIPV TV Q Sbjct: 181 LYSAHNPKIKAGVAWYGRLVGNNNELTPNNPVDIAAKLKTPVLGLYGAQDGGIPVTTVVQ 240 Query: 225 MKEALAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 M++AL+K +KSEFV+F NSGHAF+ADYR SYVEADAKEGW+R ++WFK+HGV Sbjct: 241 MQDALSKGKSKSEFVLFKNSGHAFHADYRSSYVEADAKEGWQRCIDWFKTHGV 293 >F1W331.1 F1W331_9BURK Putative carboxymethylenebutenolidase (Dienelactone hydrolase) Length = 295 Score = 370 bits (951), Expect = e-100, Method: Compositional matrix adjust. Identities = 178/289 (61%), Positives = 213/289 (73%), Gaps = 16/289 (5%) Query: 5 KNDVDSLAGTTGLLDAGNRRTFI----------------XQTMIKTDTVGLTAGEVSVTV 48 K D SL G + D +RRTF+ QTM+ TDT GLTAG +S+TV Sbjct: 6 KQDAASLIGASAFPDGFDRRTFMKVSIGTGFAAAVMPVSAQTMVMTDTDGLTAGTISLTV 65 Query: 49 NGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIARRFAKLGYLAIAPDFFVRQGNP 108 NG++VP YRAQP GKT LPVILVISEIFGVHEHIAD+ARRFAKLGYLA+APD FVRQG+ Sbjct: 66 NGEQVPVYRAQPAGKTKLPVILVISEIFGVHEHIADVARRFAKLGYLALAPDLFVRQGDA 125 Query: 109 AAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGGNPDKIGINGFCWGGRIVWLYSA 168 A +I EL K ++SKVPD+QV DLDA VA+A NGG+ +++GI GFCWGGRI WLYSA Sbjct: 126 KAAISIPELIKNVVSKVPDQQVMTDLDAVVAYAKANGGDVERMGITGFCWGGRITWLYSA 185 Query: 169 RNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPVLGLYGGKDGGIPVATVEQMKEA 228 NP+++AG AWYG++ G KSAL P ++AP L PVLGLYG KD GI ++ MK A Sbjct: 186 HNPQVRAGVAWYGQLQGTKSALNPMSATELAPALTVPVLGLYGAKDTGISQDSIATMKAA 245 Query: 229 LAKTGNKSEFVVFPNSGHAFNADYRPSYVEADAKEGWKRAVEWFKSHGV 277 LA ++SEFVVFPNSGHAF+ADYR SYVEADAKEGWK+ + WF +HGV Sbjct: 246 LATGKSQSEFVVFPNSGHAFHADYRASYVEADAKEGWKKCLAWFAAHGV 294 >Q1LK27.1 Q1LK27_RALME Putative carboxymethylenebutenolidase (Dienelactone hydrolase) Length = 291 Score = 349 bits (895), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 165/255 (64%), Positives = 196/255 (76%), Gaps = 4/255 (1%) Query: 27 IXQTMIKTDTVGLTAGEVSVTVNGQKVPAYRAQPEGKTNLPVILVISEIFGVHEHIADIA 86 + T IKTD GLTAGEV+V NG K+P YRAQPEGKTNLPV+LVISEIFGVHEHIADI Sbjct: 36 VHATTIKTDFKGLTAGEVTVESNGFKMPVYRAQPEGKTNLPVVLVISEIFGVHEHIADIC 95 Query: 87 RRFAKLGYLAIAPDFFVRQGNPAAYPTIAELNKEIISKVPDEQVFGDLDAYVAWAGQNGG 146 RRFAKLGYLAIAP+ F RQG+P++Y TI EL II KVPD QV GDLDA V WAG NGG Sbjct: 96 RRFAKLGYLAIAPELFARQGDPSSYGTIQELQANIIQKVPDAQVMGDLDACVTWAGANGG 155 Query: 147 NPDKIGINGFCWGGRIVWLYSARNPKIKAGAAWYGRIVGEKSALQPQHPIDIAPTLKTPV 206 + ++ I GFCWGGRI WLY+A N ++KAG AWYG++VGE +AL+P++ +DI L PV Sbjct: 156 DLSRLAITGFCWGGRITWLYTAHNNRVKAGVAWYGQLVGEPTALKPRNSVDIVNELDAPV 215 Query: 207 LGLYGGKDGGIPVATVEQMKEALAKTGN----KSEFVVFPNSGHAFNADYRPSYVEADAK 262 LGLYGGKD GI VE+MK ALA + N S FVV+P +GHAFNADYR SYVE+ AK Sbjct: 216 LGLYGGKDTGITQEQVERMKAALAASSNPNAKASRFVVYPEAGHAFNADYRASYVESAAK 275 Query: 263 EGWKRAVEWFKSHGV 277 +GW+R ++W + H V Sbjct: 276 DGWQRCLDWLRQHKV 290