BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0007.p (280 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC2426 [NOU] COG1989 Type II secretory pathway, prepilin signal ... 268 1e-71 RSc2827 [NOU] COG1989 Type II secretory pathway, prepilin signal... 254 2e-67 PA4528 [NOU] COG1989 Type II secretory pathway, prepilin signal ... 246 6e-65 NMB0332 [NOU] COG1989 Type II secretory pathway, prepilin signal... 239 4e-63 NMA2156 [NOU] COG1989 Type II secretory pathway, prepilin signal... 234 1e-61 >VC2426 [NOU] COG1989 Type II secretory pathway, prepilin signal peptidase PulO and related peptidases Vch Vibrio cholerae Length = 291 Score = 268 bits (685), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 148/268 (55%), Positives = 182/268 (67%), Gaps = 5/268 (1%) Query: 12 ALAACAGLLIGSFLNVVIHRLPQMMQREAENYLAH---EAGHSLPHGERYNLWTPRSACP 68 LA GL++GSFLNVVI+RLP++M+RE A E G + P G + L PRS CP Sbjct: 13 VLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEG-KLTLSLPRSTCP 71 Query: 69 HCAAPVAPRHNIPLLGYLLLRGRCAACGTAISRRYPAVEILTALLSALTAWHFGLGLEGV 128 HC P+ NIPLL +L LRG+C+ C IS RYP +E+LTAL+S + A HF G+ V Sbjct: 72 HCQTPIRVIDNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAV 131 Query: 129 AALALLYFLIALGFIDAHTQLLPDSLTLPLLWLGL-LVNLGGVFVPLADAVIGAAAGYLV 187 A L Y LIA FID T LLPD LTLPLLW G+ L LG V L+DAVIGA AGYL Sbjct: 132 ALLFFSYVLIAATFIDFDTLLLPDQLTLPLLWGGIALALLGFSPVSLSDAVIGAMAGYLS 191 Query: 188 LWSIYWIFRLATGKEGMGYGDFKLLAALGAWLGWQALPLILVLASCTGAVVGIGMVILRK 247 LWSIYW+F+L TGKEGMGYGDFKLLAALGAWLGWQ LP+I++L+S G + G+ + +K Sbjct: 192 LWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGVIFGLIQLRQQK 251 Query: 248 HSKDQSIPFGPYLALGGVLMLLYGKSLL 275 D + PFGPYLA+ G LL+G ++ Sbjct: 252 KGIDMAFPFGPYLAIAGWFALLWGDKVI 279 >RSc2827 [NOU] COG1989 Type II secretory pathway, prepilin signal peptidase PulO and related peptidases Rso Ralstonia solanacearum Length = 294 Score = 254 bits (649), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 150/258 (58%), Positives = 185/258 (71%), Gaps = 1/258 (0%) Query: 18 GLLIGSFLNVVIHRLPQMMQREAENYLAHEAGHSLPHGERYNLWTPRSACPHCAAPVAPR 77 GLL+GSFLNVVIHRLP+M++RE NY+A LPH E YNL PRSACP C + Sbjct: 27 GLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHPEPYNLVVPRSACPSCGHQIKAV 86 Query: 78 HNIPLLGYLLLRGRCAACGTAISRRYPAVEILTALLSALTAWHFGLGLEGVAALALLYFL 137 NIP+L +L LRGRC+AC T IS RYPAVE++T +L+ WHFG VA+ ALL+FL Sbjct: 87 ENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFL 146 Query: 138 IALGFIDAHTQLLPDSLTLPLLWLGLLVNLGGVFVPLADAVIGAAAGYLVLWSIYWIFRL 197 IA IDA TQLLPD++T PLLWLGLLVNL +F L DAVIGA AGYL LW+IYW ++L Sbjct: 147 IAGSMIDADTQLLPDAITQPLLWLGLLVNLFSMFARLPDAVIGATAGYLFLWAIYWAYKL 206 Query: 198 ATGKEGMGYGDFKLLAALGAWLGWQALPLILVLASCTGAVVGIGMVILRKHSKDQSIPFG 257 G+EGMGYGDFKL+ ALGAW GWQALPL+++L+S G V G+ + I R + + FG Sbjct: 207 LRGREGMGYGDFKLMGALGAWFGWQALPLLVLLSSVVGLVFGV-VRIARGATSESPFSFG 265 Query: 258 PYLALGGVLMLLYGKSLL 275 P++A GV+ LL G L+ Sbjct: 266 PFIAGAGVIALLAGPQLV 283 >PA4528 [NOU] COG1989 Type II secretory pathway, prepilin signal peptidase PulO and related peptidases Pae Pseudomonas aeruginosa Length = 290 Score = 246 bits (627), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 147/275 (53%), Positives = 181/275 (65%), Gaps = 10/275 (3%) Query: 7 AARPXALAACA---GLLIGSFLNVVIHRLPQMMQR----EAENYLAHEAGHSLPHGERYN 59 A+ P A C GLL+GSFLNVV+HRLP+MM+R EA L E P YN Sbjct: 8 ASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPE---PKQATYN 64 Query: 60 LWTPRSACPHCAAPVAPRHNIPLLGYLLLRGRCAACGTAISRRYPAVEILTALLSALTAW 119 L P SACP C + P NIPL+ YL L G+C++C AI +RYP VE+ TALLS AW Sbjct: 65 LVLPNSACPRCGHEIRPWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAW 124 Query: 120 HFGLGLEGVAALALLYFLIALGFIDAHTQLLPDSLTLPLLWLGLLVNLGGVFVPLADAVI 179 HFG + A L L + L+A+ IDA QLLPD L LPLLWLGL+ N G+F L DA+ Sbjct: 125 HFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLVLPLLWLGLIANHFGLFASLDDALF 184 Query: 180 GAAAGYLVLWSIYWIFRLATGKEGMGYGDFKLLAALGAWLGWQALPLILVLASCTGAVVG 239 GA GYL LWS++W+F+L TGKEGMGYGDFKLLA LGAW GWQ LPL ++L+S GA++G Sbjct: 185 GAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVGAILG 244 Query: 240 IGMVILRKHSKDQSIPFGPYLALGGVLMLLYGKSL 274 + M+ LR IPFGPYLA+ G + LL+G + Sbjct: 245 VIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQI 279 >NMB0332 [NOU] COG1989 Type II secretory pathway, prepilin signal peptidase PulO and related peptidases Nme Neisseria meningitidis MC58 Length = 286 Score = 239 bits (610), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 131/264 (49%), Positives = 168/264 (63%), Gaps = 6/264 (2%) Query: 13 LAACAGLLIGSFLNVVIHRLPQMMQREAENYLAHEAGHSLPHGER--YNLWTPRSACPHC 70 LAA GLL+GSFLNVVI+R+P MM+R + L E +NL P S CP C Sbjct: 14 LAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKC 73 Query: 71 AAPVAPRHNIPLLGYLLLRGRCAACGTAISRRYPAVEILTALLSALTAWHFGLGLEGVAA 130 P+ NIP++ YLLLRG+CA+C T IS RYP +E+LT +L L AW +G + Sbjct: 74 RVPIRAWQNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGG 133 Query: 131 LALLYFLIALGFIDAHTQLLPDSLTLPLLWLGLLVNLGGVFVPLADAVIGAAAGYLVLWS 190 L L FLI+L FIDA TQ LPDS+TLPL+WLGL+ NL G FVPL AV+GA AGY LW Sbjct: 134 LVLTAFLISLTFIDADTQYLPDSMTLPLIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWL 193 Query: 191 IYWIFRLATGKEGMGYGDFKLLAALGAWLGWQALPLILVLASCTGAVVGIGMVILRKHSK 250 + +++L TGK GMG GDFKL+AALGAWLG ALP+++ ++S G V I M + +K Sbjct: 194 LCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLVAAIVM----RVAK 249 Query: 251 DQSIPFGPYLALGGVLMLLYGKSL 274 Q FGP L + G ++ S+ Sbjct: 250 GQHFAFGPALTVSGWIIFTANDSV 273 >NMA2156 [NOU] COG1989 Type II secretory pathway, prepilin signal peptidase PulO and related peptidases NmA Neisseria meningitidis Z2491 Length = 286 Score = 234 bits (598), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 128/264 (48%), Positives = 167/264 (63%), Gaps = 6/264 (2%) Query: 13 LAACAGLLIGSFLNVVIHRLPQMMQREAENYLAHEAGHSLPHGER--YNLWTPRSACPHC 70 LAA GLL+GSFLNVVI+R+P MM+R + L E +NL P S CP C Sbjct: 14 LAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKC 73 Query: 71 AAPVAPRHNIPLLGYLLLRGRCAACGTAISRRYPAVEILTALLSALTAWHFGLGLEGVAA 130 P+ NIP++ YLLLRG+CA+C T IS RYP +E+LT +L L AW +G + Sbjct: 74 RVPIRAWQNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGG 133 Query: 131 LALLYFLIALGFIDAHTQLLPDSLTLPLLWLGLLVNLGGVFVPLADAVIGAAAGYLVLWS 190 L L FLI+L FIDA TQ LPDS+TLPL+WLGL+ NL G FVPL AV+GA AGY +W Sbjct: 134 LILTAFLISLTFIDADTQYLPDSMTLPLIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWL 193 Query: 191 IYWIFRLATGKEGMGYGDFKLLAALGAWLGWQALPLILVLASCTGAVVGIGMVILRKHSK 250 + +++L G+ GMG GDFKL+AALGAWLG ALP+++ ++S G V I M + +K Sbjct: 194 LCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLVAAIVM----RVAK 249 Query: 251 DQSIPFGPYLALGGVLMLLYGKSL 274 Q FGP L + G ++ S+ Sbjct: 250 GQHFAFGPALTVSGWIIFTANDSV 273