BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0004.p (364 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2825 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp ... 463 e-130 NMB0329 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp ... 432 e-121 NMA2159 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp ... 432 e-121 PA4526 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp p... 412 e-115 XF2544 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp p... 412 e-115 >RSc2825 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Rso Ralstonia solanacearum Length = 575 Score = 463 bits (1191), Expect = e-130, Method: Compositional matrix adjust. Identities = 237/402 (58%), Positives = 282/402 (70%), Gaps = 60/402 (14%) Query: 22 FDDASAVNYLQKILLDAINLGASDLHFEPFEKHCRIRFRIDGQLRDIVEPPLSMKDKLAS 81 DDA V +LQK+ +A+ G SDLHFEPFE R+RFRIDG L + +PPL ++DK+A+ Sbjct: 173 IDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIAT 232 Query: 82 HIKIISGLDIAEKRMPQDGRMKLSLSS-------------TRAIDFRVSTLPTLFGEKIV 128 IK++S LDI+EKR+PQDGRMKL ++ RAIDFRVSTLPTLFGEKIV Sbjct: 233 RIKVLSRLDISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIV 292 Query: 129 MRILDASHSQLGIDALGYNDAQKDLLLQAIRRPYGMVLMTGPTGSGKTVSLYSCLSLLNQ 188 +RIL++S +L ID LGY QK LLL I+RPYGMVL+TGPTGSGKTVSLY+ L+ LNQ Sbjct: 293 IRILESSTERLDIDQLGYEPEQKQLLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQ 352 Query: 189 PGTNISTVEDPAEISLPGINQVNVNQRAGLTFPIALRAFLRQDPDVIMVGEIRDPETADI 248 NIST EDPAEI LPGINQVNVN +AGLTF AL++FLRQDPD+IMVGEIRD ETADI Sbjct: 353 GDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEIRDLETADI 412 Query: 249 AVKAAQTGHLVFSTLHTNDAPSTLTRLMNMGVPAFNVASA-------------------- 288 ++KAAQTGHLVFSTLHTNDAP+TLTRLMNMGV FN+AS+ Sbjct: 413 SIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKP 472 Query: 289 ---------------------------AGCRRCGDTGYKGRVGIYQVMPISEAIEGLILA 321 GC C +GYKGRVGIYQVMPI+EAI+ +ILA Sbjct: 473 GPLPKETLLDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILA 532 Query: 322 HAPAPDIEAQAVREGVLTLRQAGLQKVREGLTSLEEILACTN 363 H A I QA +EGVL+LR +GL+KV++GLTSLEE+LA TN Sbjct: 533 HGTALQIAEQARKEGVLSLRDSGLRKVKQGLTSLEEVLATTN 574 >NMB0329 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Nme Neisseria meningitidis MC58 Length = 558 Score = 432 bits (1111), Expect = e-121, Method: Compositional matrix adjust. Identities = 213/390 (54%), Positives = 276/390 (70%), Gaps = 49/390 (12%) Query: 23 DDASAVNYLQKILLDAINLGASDLHFEPFEKHCRIRFRIDGQLRDIVEPPLSMKDKLASH 82 +D ++ K L DA+ GASD+HFE +E + RIRFR+DGQLR++V+PP++++ +LAS Sbjct: 168 EDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASR 227 Query: 83 IKIISGLDIAEKRMPQDGRMKLSLSST-RAIDFRVSTLPTLFGEKIVMRILDASHSQLGI 141 IK++S LDI+EKR+PQDGRM+L+ + +DFRVSTLPTLFGEK+VMRIL++ + L I Sbjct: 228 IKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNI 287 Query: 142 DALGYNDAQKDLLLQAIRRPYGMVLMTGPTGSGKTVSLYSCLSLLNQPGTNISTVEDPAE 201 D LG+ QK LLL+AI RPYGMVL+TGPTGSGKTVSLY+CL++LN NI+T EDPAE Sbjct: 288 DQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNTESVNIATAEDPAE 347 Query: 202 ISLPGINQVNVNQRAGLTFPIALRAFLRQDPDVIMVGEIRDPETADIAVKAAQTGHLVFS 261 I+LPGINQVNVN + GLTF AL++FLRQDPD+IMVGEIRD ETADIA+KAAQTGH+VFS Sbjct: 348 INLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVFS 407 Query: 262 TLHTNDAPSTLTRLMNMGVPAFNVAS---------------------------------- 287 TLHTN+AP+TL+R++NMGV FN+AS Sbjct: 408 TLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVG 467 Query: 288 --------------AAGCRRCGDTGYKGRVGIYQVMPISEAIEGLILAHAPAPDIEAQAV 333 A GC RC GYKGR G+Y+VMPISE ++ +I+ + DI A Sbjct: 468 FTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAY 527 Query: 334 REGVLTLRQAGLQKVREGLTSLEEILACTN 363 +EG++ LR+AG+ KV +G+TSLEE+ A TN Sbjct: 528 KEGMVDLRRAGILKVMQGITSLEEVTANTN 557 >NMA2159 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB NmA Neisseria meningitidis Z2491 Length = 558 Score = 432 bits (1111), Expect = e-121, Method: Compositional matrix adjust. Identities = 213/390 (54%), Positives = 276/390 (70%), Gaps = 49/390 (12%) Query: 23 DDASAVNYLQKILLDAINLGASDLHFEPFEKHCRIRFRIDGQLRDIVEPPLSMKDKLASH 82 +D ++ K L DA+ GASD+HFE +E + RIRFR+DGQLR++V+PP++++ +LAS Sbjct: 168 EDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASR 227 Query: 83 IKIISGLDIAEKRMPQDGRMKLSLSST-RAIDFRVSTLPTLFGEKIVMRILDASHSQLGI 141 IK++S LDI+EKR+PQDGRM+L+ + +DFRVSTLPTLFGEK+VMRIL++ + L I Sbjct: 228 IKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNI 287 Query: 142 DALGYNDAQKDLLLQAIRRPYGMVLMTGPTGSGKTVSLYSCLSLLNQPGTNISTVEDPAE 201 D LG+ QK LLL+AI RPYGMVL+TGPTGSGKTVSLY+CL++LN NI+T EDPAE Sbjct: 288 DQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNTESVNIATAEDPAE 347 Query: 202 ISLPGINQVNVNQRAGLTFPIALRAFLRQDPDVIMVGEIRDPETADIAVKAAQTGHLVFS 261 I+LPGINQVNVN + GLTF AL++FLRQDPD+IMVGEIRD ETADIA+KAAQTGH+VFS Sbjct: 348 INLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVFS 407 Query: 262 TLHTNDAPSTLTRLMNMGVPAFNVAS---------------------------------- 287 TLHTN+AP+TL+R++NMGV FN+AS Sbjct: 408 TLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVG 467 Query: 288 --------------AAGCRRCGDTGYKGRVGIYQVMPISEAIEGLILAHAPAPDIEAQAV 333 A GC RC GYKGR G+Y+VMPISE ++ +I+ + DI A Sbjct: 468 FTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAY 527 Query: 334 REGVLTLRQAGLQKVREGLTSLEEILACTN 363 +EG++ LR+AG+ KV +G+TSLEE+ A TN Sbjct: 528 KEGMVDLRRAGILKVMQGITSLEEVTANTN 557 >PA4526 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Pae Pseudomonas aeruginosa Length = 566 Score = 412 bits (1060), Expect = e-115, Method: Compositional matrix adjust. Identities = 204/382 (53%), Positives = 269/382 (70%), Gaps = 47/382 (12%) Query: 23 DDASAVNYLQKILLDAINLGASDLHFEPFEKHCRIRFRIDGQLRDIVEPPLSMKDKLASH 82 DDA V ++ K+LLDAI G+SDLHFEP+EK R+RFR DG L ++ +PP+ + ++++ Sbjct: 180 DDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR 239 Query: 83 IKIISGLDIAEKRMPQDGRMKLSLSSTRAIDFRVSTLPTLFGEKIVMRILDASHSQLGID 142 +K+++GLDI+E+R PQDGR+K+ +S T++IDFRV+TLPTL+GEKIVMRILD+S +Q+GID Sbjct: 240 LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGID 299 Query: 143 ALGYNDAQKDLLLQAIRRPYGMVLMTGPTGSGKTVSLYSCLSLLNQPGTNISTVEDPAEI 202 ALGY + QK+L L A+++P GM+L+TGPTGSGKTVSLY+ L++LN NIST EDP EI Sbjct: 300 ALGYEEDQKELYLAALKQPQGMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEI 359 Query: 203 SLPGINQVNVNQRAGLTFPIALRAFLRQDPDVIMVGEIRDPETADIAVKAAQTGHLVFST 262 +L GINQVNVN R G+ F ALRAFLRQDPDVIMVGEIRD ETA+IA+KAAQTGH+V ST Sbjct: 360 NLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLETAEIAIKAAQTGHMVMST 419 Query: 263 LHTNDAPSTLTRLMNMGVPAFNVASA---------------------------------- 288 LHTN A TLTRL+NMGVPAFN+A++ Sbjct: 420 LHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFP 479 Query: 289 ------------AGCRRCGDTGYKGRVGIYQVMPISEAIEGLILAHAPAPDIEAQAVREG 336 GC C + GYKGRVGIY+V+ + A++ +I+ + +I QA +EG Sbjct: 480 EDKIGTFKLYSPVGCDHCKN-GYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEG 538 Query: 337 VLTLRQAGLQKVREGLTSLEEI 358 LR +GL K +G+TSLEE+ Sbjct: 539 FNDLRTSGLLKAMQGITSLEEV 560 >XF2544 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Xfa Xylella fastidiosa 9a5c Length = 577 Score = 412 bits (1058), Expect = e-115, Method: Compositional matrix adjust. Identities = 205/387 (52%), Positives = 260/387 (67%), Gaps = 48/387 (12%) Query: 23 DDASAVNYLQKILLDAINLGASDLHFEPFEKHCRIRFRIDGQLRDIVEPPLSMKDKLASH 82 DD V ++ K+L+DAI GASD+HFEP+E+ R+RFRIDG L+ + + P + ++A+ Sbjct: 190 DDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKGVAKAPNKLNQRIAAR 249 Query: 83 IKIISGLDIAEKRMPQDGRMKLSLSSTRAIDFRVSTLPTLFGEKIVMRILDASHSQLGID 142 +K++S LDIAEKR+PQDGR+KL++S T+ +DFRVSTLPTLFGEK+V+RILD S ++LGID Sbjct: 250 LKVMSQLDIAEKRVPQDGRIKLNVSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGID 309 Query: 143 ALGYNDAQKDLLLQAIRRPYGMVLMTGPTGSGKTVSLYSCLSLLNQPGTNISTVEDPAEI 202 LGY Q+ L ++AI +PYGMVL+TGPTGSGKTVSLY+ L +LN NIST EDP EI Sbjct: 310 KLGYEADQQTLFMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEI 369 Query: 203 SLPGINQVNVNQRAGLTFPIALRAFLRQDPDVIMVGEIRDPETADIAVKAAQTGHLVFST 262 LPG+NQV N + G+TF ALR+FLRQDPDVIMVGEIRD ETA+IA+KAAQTGH+V ST Sbjct: 370 RLPGVNQVQQNNKRGMTFAAALRSFLRQDPDVIMVGEIRDLETAEIAIKAAQTGHMVLST 429 Query: 263 LHTNDAPSTLTRLMNMGVPAFNVASA---------------------------------- 288 LHTNDAP T+ RLMNMG+ +N+ S+ Sbjct: 430 LHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPTNALLAEGFT 489 Query: 289 -------------AGCRRCGDTGYKGRVGIYQVMPISEAIEGLILAHAPAPDIEAQAVRE 335 GC C + GYKGR GIYQVMPI+E I +ILA A I +A R Sbjct: 490 QEQIDAGLELYEPVGCDECTE-GYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRI 548 Query: 336 GVLTLRQAGLQKVREGLTSLEEILACT 362 G+ LRQ+ L KV +G+T L EI T Sbjct: 549 GINDLRQSALLKVAQGITGLAEINRVT 575