BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0003.p (425 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2824 [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Rso ... 480 e-135 VC0558 [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Vch V... 334 3e-91 YPO3298 [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Ype ... 313 4e-85 yfjDm [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Eco Es... 311 3e-84 ZyfjDm [P] COG4536 Putative Mg2+ and Co2+ transporter CorB EcZ E... 308 2e-83 >RSc2824 [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Rso Ralstonia solanacearum Length = 425 Score = 480 bits (1235), Expect = e-135, Method: Compositional matrix adjust. Identities = 240/419 (57%), Positives = 311/419 (74%), Gaps = 5/419 (1%) Query: 2 QILALALLILCSAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLILI 61 Q+ A+ LL+ CSAFFS++ET+LMA NRHRLRHLAK A LL +TD+LLSLILI Sbjct: 9 QLGAVVLLLCCSAFFSISETSLMALNRHRLRHLAKSNVAGARRTQGLLGQTDKLLSLILI 68 Query: 62 ANTLINALATALVTALAITAFGNHEKVITIATACVAGLLIVFAEIVPKIIGATYPERISL 121 N LIN L+ LAI FGN ++IATA VA L+IVF EI PKI GATYPERIS Sbjct: 69 GNNLINTAVPVLIANLAIHYFGNSGTTLSIATAIVAFLIIVFCEIAPKIAGATYPERISF 128 Query: 122 FTGFFLKPLMALAKPLLWVVNIIVSTILRIIHIKASGRSHEQRLSPEELRSIVLEGGNFI 181 F + PL+ LA PL+ VVN V+ +LR++ I ++ EQR+S EELR++VLE GNFI Sbjct: 129 PASFIIAPLLRLATPLVTVVNAFVTVLLRLVRINPR-QAPEQRMSAEELRTLVLESGNFI 187 Query: 182 PQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYEGEI 241 P KH+SILLNLFDL+ I+V+DVMTPRA+VE+L+LS I+E+ QL TCYHNKLPV+E + Sbjct: 188 PHKHRSILLNLFDLDAITVDDVMTPRARVESLDLSRPIEEVIQQLETCYHNKLPVFEQDT 247 Query: 242 NQIVGILHVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYFQENHERLGII 301 +Q++GILHVRKA+ L E+ ++ R+LL+ PYF+P T VF QLQYFQEN R+G++ Sbjct: 248 DQVLGILHVRKALSLLGH-AELIHDDFRSLLAKPYFVPSGTPVFRQLQYFQENRRRIGLV 306 Query: 302 VDEYGEVQGLVTLEDIIEEMIGEFTTSVPGAARADSFAWNKDGECLLEGATTLRDINKRL 361 ++EYG++ GLVTLEDIIEEMIGEFTT++P A + AW+ L + +LRD+N+RL Sbjct: 307 INEYGDMLGLVTLEDIIEEMIGEFTTTLPNAGK---LAWDAQNAYLADAGMSLRDLNRRL 363 Query: 362 GLSLPLDGPKTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQNQAIKVVKLFAPTTAK 420 GL LP DGPKTLNGL+LE L +IPEA VSVRIAGC+++IVQ+ +Q+I+ V+L P + Sbjct: 364 GLQLPTDGPKTLNGLVLEVLEEIPEAPVSVRIAGCVMDIVQMDSQSIRTVRLHRPAAGQ 422 >VC0558 [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Vch Vibrio cholerae Length = 426 Score = 334 bits (856), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 175/413 (42%), Positives = 265/413 (64%), Gaps = 4/413 (0%) Query: 6 LALLILCSAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLILIANTL 65 LA LI+ SA+FS +ET +MA NR+RL+HLA G+K A + LL+R DRL+ LILI N L Sbjct: 13 LACLIIISAYFSGSETGMMALNRYRLKHLANSGHKGAKRVEKLLDRPDRLIGLILIGNNL 72 Query: 66 INALATALVTALAITAFGNHEKVITIATACVAGLLIVFAEIVPKIIGATYPERISLFTGF 125 +N LA+A+ T + + +G+ + IAT + +++VFAE+ PK + A YPER+S + Sbjct: 73 VNILASAIATIIGMRLYGDLG--VAIATGALTLVILVFAEVTPKTLAALYPERVSYASSV 130 Query: 126 FLKPLMALAKPLLWVVNIIVSTILRIIHIKASGRSHEQRLSPEELRSIVLEGGNFIPQKH 185 L LM L PL+ VN++ + ++RI+ I LS EELR++V E G IP++H Sbjct: 131 LLTILMKLLSPLVLFVNLMTNGLIRILGISPK-HGKGDHLSSEELRTVVNEAGGLIPRRH 189 Query: 186 KSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYEGEINQIV 245 + +LL++ DLE ++V D+M PR ++ +N++ I QL H ++ +Y +I+++V Sbjct: 190 QDMLLSILDLEHVTVNDIMIPRNEITGININDDWKSIVRQLVHSPHGRVVLYRDKIDEVV 249 Query: 246 GILHVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYFQENHERLGIIVDEY 305 GIL +R+A + + E + YFIP+ T + QL FQ N ER+G+IVDEY Sbjct: 250 GILRLREASRFMLEKNDFNKEMLLRAADEIYFIPEGTPLNVQLLKFQRNKERIGLIVDEY 309 Query: 306 GEVQGLVTLEDIIEEMIGEFTTSVPGAARADSFAWNKDGECLLEGATTLRDINKRLGLSL 365 GE+ GL TLEDI+EE++GEFTTS+ + AD DG L+EG+ +RDINK L L Sbjct: 310 GEIIGLTTLEDILEEIVGEFTTSM-SPSLADEITPQGDGSFLIEGSANIRDINKSLKWKL 368 Query: 366 PLDGPKTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQNQAIKVVKLFAPTT 418 P DGP+TLNGL+LE L +IP +++SV++A +EI++++ IK+VK++ T Sbjct: 369 PTDGPRTLNGLILEHLEEIPASHLSVKVAQHKMEILELEENRIKLVKVYPRKT 421 >YPO3298 [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Ype Yersinia pestis Length = 427 Score = 313 bits (803), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 159/408 (38%), Positives = 263/408 (64%), Gaps = 5/408 (1%) Query: 13 SAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLILIANTLINALATA 72 SA+FS +ET +M NR+R+RHL+K GN+ A + LL R DRL+SL+LI N L+N LA+A Sbjct: 21 SAYFSASETGMMTLNRYRMRHLSKQGNRAARRVEKLLNRPDRLISLVLIGNNLVNILASA 80 Query: 73 LVTALAITAFGNHEKVITIATACVAGLLIVFAEIVPKIIGATYPERISLFTGFFLKPLMA 132 L T + I +G + + IAT + ++++FAE++PK I A YPER++ + L PL Sbjct: 81 LATIVGIRLYG--DAGVAIATGVLTFVILIFAEVLPKTIAALYPERVAFPSSMLLAPLQK 138 Query: 133 LAKPLLWVVNIIVSTILRIIHIKASGRSHEQRLSPEELRSIVLEGGNFIPQKHKSILLNL 192 + PL+W++N I ++ + I+ + S + +S +ELR+IV E + I ++++ +L+++ Sbjct: 139 IMLPLVWLLNTITRGLMHLCGIRGNVHSSDA-MSKDELRTIVSESQSKISRRNQDMLISV 197 Query: 193 FDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYEGEINQIVGILHVRK 252 DLEK++V D+M PR +V ++++ I QL H ++ +Y ++ +G+L VR+ Sbjct: 198 LDLEKVTVSDIMVPRNEVVGIDINDDWKSIMRQLTHSPHGRIVLYRQSLDDAIGMLRVRE 257 Query: 253 AIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYFQENHERLGIIVDEYGEVQGLV 312 A L ++ +E E + YFIP+ T + QL FQ N +++G+IVDEYG++QGLV Sbjct: 258 AYRLMTEKKEFNKENLLRAADEIYFIPEGTPLNVQLVKFQRNKKKVGMIVDEYGDIQGLV 317 Query: 313 TLEDIIEEMIGEFTTSVPGAARADSFAWNKDGECLLEGATTLRDINKRLGLSLPLDGPKT 372 T+EDI+EE++G+FTTS+ A+ DG L++G+ ++R++NK SLP+D +T Sbjct: 318 TVEDILEEIVGDFTTSM-SPTLAEEVNPQSDGSVLIDGSASVREVNKAFNWSLPVDA-RT 375 Query: 373 LNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQNQAIKVVKLFAPTTAK 420 +NG+LLE L +IP+A++ VR+ IE++ VQ IK VK+ T+A+ Sbjct: 376 INGMLLEELEEIPQAHIHVRLGNYQIEVLDVQENMIKRVKITPMTSAE 423 >yfjDm [P] COG4536 Putative Mg2+ and Co2+ transporter CorB Eco Escherichia coli K12 Length = 420 Score = 311 bits (796), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 162/406 (39%), Positives = 260/406 (64%), Gaps = 12/406 (2%) Query: 13 SAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLILIANTLINALATA 72 SA+FS +ET +M NR+RLRH+AK GN+ A + LL + DRL+SL+LI N L+N LA+A Sbjct: 12 SAYFSGSETGMMTLNRYRLRHMAKQGNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASA 71 Query: 73 LVTALAITAFGNHEKVITIATACVAGLLIVFAEIVPKIIGATYPERISLFTGFFLKPLMA 132 L T + + +G+ + IAT + +++VFAE++PK I A YPE+++ + F L PL Sbjct: 72 LGTIVGMRLYGDAG--VAIATGVLTFVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQI 129 Query: 133 LAKPLLWVVNIIVSTILRIIHIKA----SGRSHEQRLSPEELRSIVLEGGNFIPQKHKSI 188 L PL+W++N I ++R++ IK SG LS EELR+IV E + I ++++ + Sbjct: 130 LMMPLVWLLNAITRMLMRMMGIKTDIVVSGS-----LSKEELRTIVHESRSQISRRNQDM 184 Query: 189 LLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYEGEINQIVGIL 248 LL++ DLEK++V+D+M PR+++ ++++ I QL+ H ++ +Y ++ + +L Sbjct: 185 LLSVLDLEKMTVDDIMVPRSEIIGIDINDDWKSILRQLSHSPHGRIVLYRDSLDDAISML 244 Query: 249 HVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYFQENHERLGIIVDEYGEV 308 VR+A L S+ +E T E + YF+P+ T + TQL FQ N +++G++V+EYG++ Sbjct: 245 RVREAWRLMSEKKEFTKETMLRAADEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDI 304 Query: 309 QGLVTLEDIIEEMIGEFTTSVPGAARADSFAWNKDGECLLEGATTLRDINKRLGLSLPLD 368 QGLVT+EDI+EE++G+FTTS+ A+ DG +++G +R+INK LP D Sbjct: 305 QGLVTVEDILEEIVGDFTTSM-SPTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPED 363 Query: 369 GPKTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQNQAIKVVKLF 414 +T+NG++LE L +IP A VRI I+I+ VQ+ IK VK+F Sbjct: 364 DARTVNGVILEALEEIPVAGTRVRIGEYDIDILDVQDNMIKQVKVF 409 >ZyfjDm [P] COG4536 Putative Mg2+ and Co2+ transporter CorB EcZ Escherichia coli O157:H7 EDL933 Length = 420 Score = 308 bits (788), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 161/406 (39%), Positives = 259/406 (63%), Gaps = 12/406 (2%) Query: 13 SAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLILIANTLINALATA 72 SA+FS +ET +M NR+RLRH+AK GN+ A + LL + DRL+SL+LI N L+N LA+A Sbjct: 12 SAYFSGSETGMMTLNRYRLRHMAKQGNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASA 71 Query: 73 LVTALAITAFGNHEKVITIATACVAGLLIVFAEIVPKIIGATYPERISLFTGFFLKPLMA 132 L T + + +G+ + IAT + +++VFAE++PK I A YPE+++ + F L PL Sbjct: 72 LGTIVGMRLYGDAG--VAIATGVLTFVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQI 129 Query: 133 LAKPLLWVVNIIVSTILRIIHIKA----SGRSHEQRLSPEELRSIVLEGGNFIPQKHKSI 188 L PL+W++N I ++R++ IK SG LS EELR+IV E + I ++++ + Sbjct: 130 LMMPLVWLLNAITRMLMRMMGIKTDIVVSGS-----LSKEELRTIVHESRSQISRRNQDM 184 Query: 189 LLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYEGEINQIVGIL 248 LL++ DLEK++V+D+M PR+++ ++++ I QL+ H ++ +Y ++ + +L Sbjct: 185 LLSVPDLEKMTVDDIMVPRSEIIGIDINDDWKSILRQLSHSPHGRIVLYRDSLDDAISML 244 Query: 249 HVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYFQENHERLGIIVDEYGEV 308 VR+A L S+ +E T E + YF+P+ T + TQL FQ N +++G++V+EYG++ Sbjct: 245 RVREAWRLMSEKKEFTKETMLRAADXIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDI 304 Query: 309 QGLVTLEDIIEEMIGEFTTSVPGAARADSFAWNKDGECLLEGATTLRDINKRLGLSLPLD 368 QGLVT+EDI+EE++G+FTTS+ A+ DG +++G +R+INK LP D Sbjct: 305 QGLVTVEDILEEIVGDFTTSM-SPTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPED 363 Query: 369 GPKTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQNQAIKVVKLF 414 +T+NG++LE L +IP VRI I+I+ VQ+ IK VK+F Sbjct: 364 DARTVNGVILEALEEIPVVGTRVRIGEYDIDILDVQDNMIKQVKVF 409