BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0400.p (263 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc3138 [IQR] COG1028 Dehydrogenases with different specificitie... 340 1e-93 PA1344 [IQR] COG1028 Dehydrogenases with different specificities... 282 5e-76 BS_yvaG [IQR] COG1028 Dehydrogenases with different specificitie... 261 1e-69 BS_yvrD [IQR] COG1028 Dehydrogenases with different specificitie... 239 7e-63 SMb21010 [IQR] COG1028 Dehydrogenases with different specificiti... 140 2e-33 >RSc3138 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 262 Score = 340 bits (873), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 172/264 (65%), Positives = 201/264 (76%), Gaps = 3/264 (1%) Query: 1 MDLQLKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSETNGT-V 59 MDL L+ K ALV+GST GIG+AIA LA EGA VI+NGRT ASV EA R+ +E G Sbjct: 1 MDLHLQNKLALVTGSTKGIGHAIAVALAAEGARVILNGRTDASVAEAVSRLLAEVPGAHA 60 Query: 60 QGYAGDLSKPDAANEVIQRHPGINILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGVRL 119 + +AGDLS P A + R P ++ILVNNLGIF+PK FEDI D +WLRFF VNV+SGVRL Sbjct: 61 EAFAGDLSDPAQARALAARFPRVDILVNNLGIFDPKPFEDIDDAEWLRFFSVNVMSGVRL 120 Query: 120 ARLVLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVSRGLAESVAGTGITVN 179 +R LP MK NWGRI+FISSES VQIP EM+HYG+TKTA L +SRGLAE AGT +TVN Sbjct: 121 SRAYLPGMKAQNWGRIVFISSESGVQIPAEMVHYGVTKTALLGLSRGLAELCAGTAVTVN 180 Query: 180 SILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLVAYV 239 ++LPGPT+S GV F + + + FE E FF+ RPTSLIKRF TPQEVASLVAYV Sbjct: 181 AVLPGPTRSEGVEAFTRKL--SGGQGFEAFEKAFFETARPTSLIKRFATPQEVASLVAYV 238 Query: 240 VSPLASATTGAALRVDGGVIKSAF 263 SPL+SATTGAALRVDGGV+KSAF Sbjct: 239 ASPLSSATTGAALRVDGGVVKSAF 262 >PA1344 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 264 Score = 282 bits (722), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 142/262 (54%), Positives = 186/262 (70%), Gaps = 1/262 (0%) Query: 1 MDLQLKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRS-ETNGTV 59 M + L K A+VSGSTAGIG+AIA LA GA V+VNGR+QA+VD A IR+ + + V Sbjct: 1 MQIDLSDKLAIVSGSTAGIGFAIASGLAAAGATVVVNGRSQAAVDRAIAAIRNGQPDARV 60 Query: 60 QGYAGDLSKPDAANEVIQRHPGINILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGVRL 119 +G AGDL + + P +ILVNNLGIFE K F DI D +W RFF+VNV+SGVRL Sbjct: 61 RGVAGDLGTAEGCAAFVAAEPKADILVNNLGIFELKDFFDIEDAEWSRFFEVNVMSGVRL 120 Query: 120 ARLVLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVSRGLAESVAGTGITVN 179 AR +P M WGR++F+SSESA+ IP +MIHYG +KTA L+VSRGLA+ +AGTG+TVN Sbjct: 121 ARAYVPGMVERGWGRVVFLSSESALNIPADMIHYGFSKTANLSVSRGLAKRLAGTGVTVN 180 Query: 180 SILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLVAYV 239 ++LPGPT + GV + + + A+ + ++ E+ F R +S+I+R TP+EVA+LV Y Sbjct: 181 AVLPGPTLTEGVAEMLREDAQKAGQSIEEAGTAFVRQHRSSSIIQRLATPEEVANLVVYT 240 Query: 240 VSPLASATTGAALRVDGGVIKS 261 S ASATTGAALRVDGGV+ S Sbjct: 241 CSTQASATTGAALRVDGGVLDS 262 >BS_yvaG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 264 Score = 261 bits (666), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 126/264 (47%), Positives = 177/264 (67%), Gaps = 1/264 (0%) Query: 1 MDLQLKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSE-TNGTV 59 M L L+GKTALV+GST+GIG AIA +LA EGA VI+NGR + V++ + ++++ + Sbjct: 1 MKLNLQGKTALVTGSTSGIGKAIASSLAEEGAAVIINGRREEKVNQTIDELKTQHAEAVL 60 Query: 60 QGYAGDLSKPDAANEVIQRHPGINILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGVRL 119 A DL + NE+ Q +P ++ILVNNLGIFEP + DIPD++W RFF+VN++SGVRL Sbjct: 61 YPAAFDLGTEEGCNELFQAYPEVDILVNNLGIFEPAEYFDIPDDEWFRFFEVNIMSGVRL 120 Query: 120 ARLVLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVSRGLAESVAGTGITVN 179 R L M GR+IFI+SE+A+ EM HY TKT QL++SR LAE GT +TVN Sbjct: 121 TRRYLHNMIEKKEGRVIFIASEAAIMPSQEMAHYSATKTMQLSISRSLAELATGTNVTVN 180 Query: 180 SILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLVAYV 239 +++PG T + GV + + + T ++ EA F RPTS+I+R P+E+A V ++ Sbjct: 181 TVMPGSTLTEGVETMLNSLYPGENLTVQEAEARFMKENRPTSIIQRLIRPEEIAHFVTFL 240 Query: 240 VSPLASATTGAALRVDGGVIKSAF 263 SPL+SA GAALR DGG+++S F Sbjct: 241 SSPLSSAINGAALRADGGLVRSVF 264 >BS_yvrD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 263 Score = 239 bits (609), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 130/265 (49%), Positives = 178/265 (67%), Gaps = 7/265 (2%) Query: 1 MDLQLKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSETNGTVQ 60 MDLQLK K L++GST+GIG A A + +EGA VIVNGR Q +VD E + GTV Sbjct: 1 MDLQLKEKLVLITGSTSGIGKAAAKSFLQEGAAVIVNGRKQETVDRTIEELSG--YGTVH 58 Query: 61 GYAGDLSKPDAANEVIQRHP---GINILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGV 117 G A DLSK D A I++ I+ILVNNLG FE K F D+ DE+W ++F+VNV+S V Sbjct: 59 GAAADLSKTDEAAAFIEKVNEIGDIDILVNNLGFFEVKDFADVTDEEWNQYFEVNVMSAV 118 Query: 118 RLARLVLPEMKRTNWGRIIFISSESAVQ-IPTEMIHYGMTKTAQLAVSRGLAESVAGTGI 176 R +R LP+M N GRI+ I+SE+ V+ +PT MI Y MTKTA +++SRG+AE GT + Sbjct: 119 RTSRHFLPKMLAKNSGRILNIASEAGVKPLPT-MIPYSMTKTALISLSRGMAEMTKGTNV 177 Query: 177 TVNSILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLV 236 TVNS+LPGPT + GV ++E A+A+ + + ++F PTSLI+R+ T +EVA+ + Sbjct: 178 TVNSVLPGPTWTEGVASYMEGAAQAAGQDTDTFIKDYFKVNEPTSLIQRYATAEEVANTI 237 Query: 237 AYVVSPLASATTGAALRVDGGVIKS 261 ++ S ASA G A RV+GG+I+S Sbjct: 238 VFLASDAASAINGTAQRVEGGIIRS 262 >SMb21010 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 258 Score = 140 bits (354), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 94/255 (36%), Positives = 135/255 (52%), Gaps = 8/255 (3%) Query: 8 KTALVSGSTAGIGYAIAHTLAREGANVIVNG-RTQASVDEAAERIRSETNGTVQGYAGDL 66 KTA+++GST+GIG AIA TLA+ GAN+++NG + + + ++GTV + D+ Sbjct: 3 KTAVITGSTSGIGLAIARTLAKAGANIVLNGFGAPDEIRTVTDEVAGLSSGTVLHHPADM 62 Query: 67 SKP----DAANEVIQRHPGINILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGVRLARL 122 +KP D V R G +ILVNN G+ + ED P E W R VN+ S R Sbjct: 63 TKPSEIADMMAMVADRFGGADILVNNAGVQFVEKIEDFPVEQWDRIIAVNLSSSFHTIRG 122 Query: 123 VLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVSRGLAESVAGTGITVNSIL 182 +P MK+ WGRII I+S + Y K + +++ +A VA +G+TVNSI Sbjct: 123 AIPPMKKKGWGRIINIASAHGLVASPFKSAYVAAKHGIMGLTKTVALEVAESGVTVNSIC 182 Query: 183 PGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLVAYVVSP 242 PG + V + D AR T EQV E +PT K+F T ++VASL Y+ Sbjct: 183 PGYVLTPLVEKQIPDQARTRGITEEQVINEVMLKGQPT---KKFITVEQVASLALYLAGD 239 Query: 243 LASATTGAALRVDGG 257 A+ TG + +DGG Sbjct: 240 DAAQITGTHVSMDGG 254