BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0376.p (464 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 682 0.0 PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 525 e-148 Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 485 e-136 AGl2773 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 277 3e-74 BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 276 8e-74 >PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 462 Score = 682 bits (1759), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/450 (69%), Positives = 381/450 (84%) Query: 6 KKEIRLNAFNMNCVNHINHGLWTHPRDRSQDYTDIAYWTDLAKLLERGKFDGLFLADIVG 65 KK+I LNAF+MNCV HINHGLWTHPRDRS DY + +WT+LA+LLERG FDGLF+ADI+G Sbjct: 3 KKQILLNAFSMNCVGHINHGLWTHPRDRSSDYRKLRHWTELARLLERGLFDGLFIADILG 62 Query: 66 VYDVYQNSVDITLRESIQLPVNDPLLLISAMGFVTEHLGFGITANLTYEAPFLFARRMST 125 VYDVYQ +++T RE++QLPVNDPL+L+SAM VTEHLGFG+TANLTYEAP+ FARR+ST Sbjct: 63 VYDVYQGGIEVTAREAVQLPVNDPLMLVSAMAGVTEHLGFGVTANLTYEAPYPFARRLST 122 Query: 126 LDHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSWEDDAV 185 LDHL+ GR+GWNIVTGYL+SAA+AMG S Q+EHD+RYDRADE++EV+YKLWEGSWEDDAV Sbjct: 123 LDHLSDGRIGWNIVTGYLESAAKAMGQSAQIEHDRRYDRADEYLEVLYKLWEGSWEDDAV 182 Query: 186 IRDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLSEPSRQRTPVLYQAGSSGRGQAFGARHA 245 + DRQ R+YA+P+K+HKI+H G++Y+VEGYHL+EPS QRTPVL+QAGSS RG AF ARHA Sbjct: 183 LADRQRRVYARPEKIHKIRHHGEFYQVEGYHLAEPSPQRTPVLFQAGSSERGLAFAARHA 242 Query: 246 ECVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEKEAREKYAEYAR 305 ECVFVSGQ++EA R+LV IR V GR+A+D++IFMG+ V+VA T EAR+K+AEY R Sbjct: 243 ECVFVSGQTREATRQLVQRIRAAVVGAGRRAEDIRIFMGINVIVAATAAEARDKHAEYRR 302 Query: 306 YASPEAGLAHFSSGTGIDFSQYELDEPIRQVKTNSIESLVKTVTNPELNWTKRKLLEQLE 365 YASPEAGLAHF+S +GID S+Y LDEPIR K N+IES+VK +T + T RKLL+QL Sbjct: 303 YASPEAGLAHFASSSGIDLSRYGLDEPIRYEKNNAIESVVKNLTVARTDSTVRKLLDQLA 362 Query: 366 LGGRYATIIGSPQQVADELEAWIRETDIDGFNLTRTVTPESFEAFVDLVIPELQTRGSYK 425 LGGRY T++G P+QVAD+LE WI +T +DGFNLTRTV PES+ F+DLV+PELQ RGSYK Sbjct: 363 LGGRYVTLVGDPRQVADDLEGWIADTGLDGFNLTRTVEPESYADFIDLVVPELQARGSYK 422 Query: 426 HDYAPGTLREKLFTGSGARLPQQHTGARYR 455 YAPGTLREKLF ARL +H GA +R Sbjct: 423 TAYAPGTLREKLFGDGRARLQAEHAGAAFR 452 >PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 469 Score = 525 bits (1351), Expect = e-148, Method: Compositional matrix adjust. Identities = 247/451 (54%), Positives = 326/451 (72%), Gaps = 3/451 (0%) Query: 7 KEIRLNAFNMNCVNHINHGLWTHPRDRSQDYTDIAYWTDLAKLLERGKFDGLFLADIVGV 66 +EIRLNAF MNCV H + GLW HPRDRS Y D+ YWTDLA+LLERG+FDGLF+AD++G+ Sbjct: 3 REIRLNAFEMNCVGHQSPGLWAHPRDRSSQYKDLEYWTDLARLLERGRFDGLFIADVLGI 62 Query: 67 YDVYQNSVDITLRESIQLPVNDPLLLISAMGFVTEHLGFGITANLTYEAPFLFARRMSTL 126 YDV + D +R++ Q+PVNDPL LI+ M VT HLGFG+TA+L++E P+ FARR+STL Sbjct: 63 YDVLDGNGDAAIRQAAQVPVNDPLALITPMAAVTRHLGFGLTASLSFEHPYPFARRLSTL 122 Query: 127 DHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSWEDDAVI 186 DHLTKGR+GWN+VT YL+S AR +G QL+HD+RYD ADE+++V+YKL+EGSWED AV+ Sbjct: 123 DHLTKGRIGWNVVTSYLESGARNLGQQAQLDHDRRYDYADEYLQVLYKLFEGSWEDGAVL 182 Query: 187 RDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLSEPSRQRTPVLYQAGSSGRGQAFGARHAE 246 RDR+ RI++ P K+H+I+H G++++V G HL EPS QRTPVLYQAG+S RG+ F A HAE Sbjct: 183 RDRRRRIFSDPGKIHEIRHVGEHFQVPGIHLCEPSPQRTPVLYQAGASSRGKRFAAEHAE 242 Query: 247 CVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEKEAREKYAEYARY 306 CVFV+ SK +++ V IR+ GR V +F TVV+ T+ +AR K+ EY + Sbjct: 243 CVFVAAPSKTVLKQAVADIRQRVAEAGRDPRKVLVFNLQTVVLGETDAKARAKFDEYRAF 302 Query: 307 ASPEAGLAHFSSGTGIDFSQYELDEPIRQVKTNSIESLVKTVT--NPELNWTKRKLLEQL 364 S E LA S TGIDF QY D+ +R + TN+I+S V+ + +P WT R+L + + Sbjct: 303 VSYEGALALISGWTGIDFGQYRPDQVLRHIHTNAIQSAVEAFSSADPTRTWTVRELADWV 362 Query: 365 ELGGRYATIIGSPQQVADELEAWIRETDIDGFNLTRTVTPESFEAFVDLVIPELQTRGSY 424 +GG +GSP VAD L+ W+ +TD+DGFNL + E+F V+L++PELQ RG Y Sbjct: 363 GIGGFGPLFVGSPATVADLLQEWVEDTDVDGFNLAYALAHETFSDVVELLVPELQKRGVY 422 Query: 425 KHDYAPGTLREKLFTGSGARLPQQHTGARYR 455 K +YAPGTLREKLF G G RLP H A YR Sbjct: 423 KTEYAPGTLREKLF-GDGPRLPDSHPAAGYR 452 >Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Cgl Corynebacterium glutamicum Length = 487 Score = 485 bits (1248), Expect = e-136, Method: Compositional matrix adjust. Identities = 236/460 (51%), Positives = 308/460 (66%), Gaps = 3/460 (0%) Query: 2 SNSDKKEIRLNAFNMNCVNHINHGLWTHPRDRSQDYTDIAYWTDLAKLLERGKFDGLFLA 61 + + ++I NAF+MNCV H + GLWTHP+D+++DY + YW LAK LE+G FDGLF+A Sbjct: 7 TTQENRKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLFDGLFIA 66 Query: 62 DIVGVYDVYQNSVDITLRESIQLPVNDPLLLISAMGFVTEHLGFGITANLTYEAPFLFAR 121 D++G YDVY +S + L Q+PVNDP+LL+SAM + T++LGFGITA YE P+ FAR Sbjct: 67 DVLGTYDVYGSSNEAALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEHPYPFAR 126 Query: 122 RMSTLDHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSWE 181 R++TLDHLT GRVGWN+VTGYL SAA+ MG ++QL HD+RYD+ADE++EV+YKL EGSWE Sbjct: 127 RLATLDHLTNGRVGWNVVTGYLPSAAQNMGDTDQLPHDERYDKADEYLEVIYKLLEGSWE 186 Query: 182 DDAVIRDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLSEPSRQRTPVLYQAGSSGRGQAFG 241 DDAV + + ++ KVH I H G Y+ V G ++EPS QRTPV+YQAG+S RG F Sbjct: 187 DDAVQNNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASPRGLKFA 246 Query: 242 ARHAECVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEKEAREKYA 301 +AE VF++ + EA+ K V IR AV GR VKIF T++ TE +A+ K Sbjct: 247 GENAEAVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEADAQAKLE 306 Query: 302 EYARYASPEAGLAHFSSGTGIDFSQYELDEPIRQVKTNSIESLVKTVTN--PELNWTKRK 359 EY+RY P GL S TG D SQY+LDEPI +++N+I+S T++N E WT RK Sbjct: 307 EYSRYIDPVGGLTLMSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEGAWTVRK 366 Query: 360 LLEQLELGGRYATIIGSPQQVADELEAWIRETDIDGFNLTRTVTPESFEAFVDLVIPELQ 419 L E +GG ++GS VA EL +D+DGFNL +TP +FE VD V+PELQ Sbjct: 367 LGEATGIGGFGPVLVGSGANVAAELARIQDLSDVDGFNLAYAITPGTFEDVVDFVVPELQ 426 Query: 420 TRGSYKHDYAPGTLREKLFTGSGARLPQQHTGARYRTNKR 459 YK +YAPG+LR KL G G RL H GA YR R Sbjct: 427 KLSRYKTEYAPGSLRNKLL-GKGDRLDDTHRGASYRLGAR 465 >AGl2773 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 450 Score = 277 bits (709), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 166/443 (37%), Positives = 239/443 (53%), Gaps = 22/443 (4%) Query: 1 MSNSDKKEIRLNAFNMNCVNHINHGLWTHPRDRSQDYTDIAYWTDLAKLLERGKFDGLFL 60 MS +K + LNAF +H W HP TDI + D+A+ E K D +F Sbjct: 1 MSRKSEKLV-LNAFIYPGGHH--EAAWRHPESEPLRVTDIKLYQDIARKAEAAKLDAIFF 57 Query: 61 ADIVGVYDVYQNSVDITLRESIQLPVNDPLLLISAMGFVTEHLGFGITANLTYEAPFLFA 120 AD V ++ R ++ P+ +++A+ VTE +GF TA+ TY P+ A Sbjct: 58 ADA----PVLDANIRYAARHRLE-----PITMLAALAAVTEKIGFIATASTTYYEPYNLA 108 Query: 121 RRMSTLDHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSW 180 R ++++DH++ GRVGWNIVT D+AA L + H RY +A EF+EVV KLW+ SW Sbjct: 109 RLLASVDHISGGRVGWNIVTTSADAAAGNFNLQQHPPHADRYRQATEFVEVVTKLWD-SW 167 Query: 181 EDDAVIRDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLSEPSRQRTPVLYQAGSSGRGQAF 240 EDDA++ DR++ ++A D++H H G++Y+V G S Q PV QAGSS G+ F Sbjct: 168 EDDAIVADRESGLFADTDRIHPASHDGEFYRVRGALNLPRSPQGRPVYVQAGSSEDGRGF 227 Query: 241 GARHAECVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEKEAREKY 300 AR+AE +F + Q+ E+ R + I+ GR VKI G++ +A TE+EAR+ Sbjct: 228 AARYAEAIFTAHQTLESARSFYSDIKRRVAATGRNPAHVKILPGISPFIASTEEEARKLE 287 Query: 301 AEYARYASPEAGLAHFSSGTGIDFSQYELDEPI--------RQVKTNSIESLVKTVTNPE 352 A + P L TGID S +LD+P+ R +S LV V + E Sbjct: 288 ASFNDLIQPAFSLGQLQRITGIDLSSADLDQPLPKEAVEATRAQADSSRHQLVLDVIDRE 347 Query: 353 LNWTKRKLLEQLELGGRYATIIGSPQQVADELEAWIRETDIDGFNLTRTVTPESFEAFVD 412 N T R+LL +L G + I G+P+QVAD +E W RE DGFN+ F+ FVD Sbjct: 348 -NPTIRQLLHRLAGGRGHKVISGTPEQVADWIETWFREGAADGFNIMPPWLNGGFDIFVD 406 Query: 413 LVIPELQTRGSYKHDYAPGTLRE 435 V+P L+ RG ++ DY TLR+ Sbjct: 407 EVVPILKQRGLFREDYEGDTLRD 429 >BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Bsu Bacillus subtilis Length = 441 Score = 276 bits (706), Expect = 8e-74, Method: Compositional matrix adjust. Identities = 158/439 (35%), Positives = 240/439 (54%), Gaps = 18/439 (4%) Query: 3 NSDKKEIRLNAFNMNCVNHINHGLWTHPRDRSQDYTDIAYWTDLAKLLERGKFDGLFLAD 62 S KK+I+L F +H+ W HP S ++ Y+ +LAK ERGK D LFLAD Sbjct: 2 TSKKKQIKLGVFLAGTGHHV--ASWRHPDAPSDASMNLDYFKELAKTAERGKLDMLFLAD 59 Query: 63 IVGVYDVYQNSVDITLRESIQLPVNDPLLLISAMGFVTEHLGFGITANLTYEAPFLFARR 122 + + +V L +P L+SA+ VT +G TA+ TY PF AR+ Sbjct: 60 SLSIDSKSHPNV---------LTRFEPFTLLSALAQVTSKIGLTATASTTYSEPFHIARQ 110 Query: 123 MSTLDHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSWED 182 ++LDHL+ GR GWN+VT ++S A + LEH RY RA+EF+E+V LW+ SWE+ Sbjct: 111 FASLDHLSNGRAGWNVVTSSIESTALNFSGEKHLEHHLRYQRAEEFVEIVKGLWD-SWEE 169 Query: 183 DAVIRDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLSEPSRQRTPVLYQAGSSGRGQAFGA 242 DA IR+++ + +K+H++ H G+Y+ V G + Q PV+ QAGSSG G+A A Sbjct: 170 DAFIRNKETGEFFDKEKMHELNHKGEYFSVRGPLNVSRTPQGQPVIIQAGSSGDGKALAA 229 Query: 243 RHAECVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEKEAREKYAE 302 + AE +F + E+ ++ I+E A GR + + I G+ ++A TE+ A+ KY E Sbjct: 230 KTAEVIFTAQNHLESAQEFYQSIKEQAAEFGRDPEKIAIMPGIFPIIADTEEAAQAKYKE 289 Query: 303 YARYASPEAGLAHFSSGT-GIDFSQYELDEPIRQV---KTNSIESLVKTVTN--PELNWT 356 P GL + GID S Y LD P+ ++ +N+++S K V N T Sbjct: 290 LQDLIIPSVGLQILQNYLGGIDLSAYPLDGPLPKLDAEASNAVKSRFKLVQEMAERDNMT 349 Query: 357 KRKLLEQLELGGRYATIIGSPQQVADELEAWIRETDIDGFNLTRTVTPESFEAFVDLVIP 416 R+L + + + +G+P+Q+AD+++ W+ DGFN+ + PE E FVD V+P Sbjct: 350 IRELYKYVAGSRGHHIFVGTPEQLADKMQEWVDTKACDGFNIMPPLLPEGIEVFVDQVVP 409 Query: 417 ELQTRGSYKHDYAPGTLRE 435 LQ RG ++ +Y TLRE Sbjct: 410 ILQERGVFRKEYEGTTLRE 428