BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0250.p (300 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0917 [E] COG0031 Cysteine synthase Rso Ralstonia solanacearum 494 e-140 PA0932 [E] COG0031 Cysteine synthase Pae Pseudomonas aeruginosa 416 e-116 VC0537 [E] COG0031 Cysteine synthase Vch Vibrio cholerae 391 e-108 STM2440 [E] COG0031 Cysteine synthase Sty Salmonella typhimurium... 387 e-107 cysM [E] COG0031 Cysteine synthase Eco Escherichia coli K12 384 e-106 >RSc0917 [E] COG0031 Cysteine synthase Rso Ralstonia solanacearum Length = 300 Score = 494 bits (1272), Expect = e-140, Method: Compositional matrix adjust. Identities = 235/300 (78%), Positives = 269/300 (89%) Query: 1 MPYLTIEDTIGNTPLIQLQRLGGEEARRRNNIILGKMEGNNPAGSVKDRAALSMIRRAEE 60 M Y TIEDTIGNTPL++LQR+ GE+ RR N+ILGK+EGNNPAGSVKDR ALSMI+ AE Sbjct: 1 MAYPTIEDTIGNTPLVRLQRIPGEDNARRGNVILGKLEGNNPAGSVKDRPALSMIKHAET 60 Query: 61 RGQIKPGDTLIEATSGNTGIALAMVAALRGYKMVLLMPENLSEERRQSMAAYGAKIVLTP 120 RG+IKPGDTLIEATSGNTGIALAM AA+RGYKMVL+MPE+LS ERRQSMAAYGA+IVLTP Sbjct: 61 RGRIKPGDTLIEATSGNTGIALAMAAAIRGYKMVLIMPEDLSVERRQSMAAYGAEIVLTP 120 Query: 121 KSGGMEYARDLAEQMQKEGQGLILDQFANGDNPQAHYETTGPELWRDTEGRITHFVSAMG 180 GGMEYARDLA+ M+++G+G+ILDQFAN DNP AHYE TGPE+WRDTEGRITHFVSAMG Sbjct: 121 VKGGMEYARDLADAMERDGKGVILDQFANPDNPLAHYEGTGPEIWRDTEGRITHFVSAMG 180 Query: 181 TTGTIMGVSRYLKEQNPGIQIIGAQPEEGSSIPGIRKWPEAYLPKIYEKPRVDQIESVSQ 240 TTGTI GVSR+LKE+NP IQI+GAQP EGS IPGIRKWPEAY+PKIY+ +D+ ESVSQ Sbjct: 181 TTGTITGVSRFLKEKNPAIQIVGAQPAEGSRIPGIRKWPEAYMPKIYDPKHIDRTESVSQ 240 Query: 241 AAAENMARKLAMVEGLFCGISAAGACEVALRISQQVENATIVFVVCDRGDRYLSTGVFPA 300 + AE+MAR++A EG+FCGISAAGA VALR+++ VENATIVFVVCDRGDRYLSTGVFPA Sbjct: 241 SDAEHMARRMAREEGIFCGISAAGALCVALRVAEAVENATIVFVVCDRGDRYLSTGVFPA 300 >PA0932 [E] COG0031 Cysteine synthase Pae Pseudomonas aeruginosa Length = 299 Score = 416 bits (1070), Expect = e-116, Method: Compositional matrix adjust. Identities = 192/296 (64%), Positives = 245/296 (82%), Gaps = 4/296 (1%) Query: 3 YLTIEDTIGNTPLIQLQRLGGEEARRRNNIILGKMEGNNPAGSVKDRAALSMIRRAEERG 62 Y TI D +GNTPL++LQRL GE + N +L K+EGNNPAGSVKDR ALSMI RAE RG Sbjct: 5 YPTIADCVGNTPLVRLQRLPGETS----NTLLVKLEGNNPAGSVKDRPALSMITRAELRG 60 Query: 63 QIKPGDTLIEATSGNTGIALAMVAALRGYKMVLLMPENLSEERRQSMAAYGAKIVLTPKS 122 I+PGDTLIEATSGNTGIALAM AA++GYKM+L+MP+N + ER+ +M AYGA+++L K Sbjct: 61 DIRPGDTLIEATSGNTGIALAMAAAIKGYKMILIMPDNSTAERKAAMTAYGAELILVSKE 120 Query: 123 GGMEYARDLAEQMQKEGQGLILDQFANGDNPQAHYETTGPELWRDTEGRITHFVSAMGTT 182 GME ARDLA+++Q+EG+G +LDQFANGDNP+AHY +TGPE+W+ T G ITHFVS+MGTT Sbjct: 121 EGMEGARDLADKLQREGRGKVLDQFANGDNPEAHYHSTGPEIWQQTGGSITHFVSSMGTT 180 Query: 183 GTIMGVSRYLKEQNPGIQIIGAQPEEGSSIPGIRKWPEAYLPKIYEKPRVDQIESVSQAA 242 GTIMGVSRYLKEQNP +QI+G QP EGS+IPGIR+WP+ YLPKIY+ RVD++ + Q Sbjct: 181 GTIMGVSRYLKEQNPAVQIVGLQPMEGSAIPGIRRWPQEYLPKIYDASRVDRVVDMHQDE 240 Query: 243 AENMARKLAMVEGLFCGISAAGACEVALRISQQVENATIVFVVCDRGDRYLSTGVF 298 AE++ R+LA EG+FCG+S+ GA LR+S+++ENA +V ++CDRGDRYLS+GV+ Sbjct: 241 AEDIMRRLAREEGIFCGVSSGGAVAAMLRLSRELENAVLVAIICDRGDRYLSSGVY 296 >VC0537 [E] COG0031 Cysteine synthase Vch Vibrio cholerae Length = 295 Score = 391 bits (1004), Expect = e-108, Method: Compositional matrix adjust. Identities = 184/296 (62%), Positives = 236/296 (79%), Gaps = 5/296 (1%) Query: 3 YLTIEDTIGNTPLIQLQRLGGEEARRRNNIILGKMEGNNPAGSVKDRAALSMIRRAEERG 62 + T+ED +G TPL++LQRL + +L K+EGNNPAGSVKDR AL+MI +AE RG Sbjct: 4 FPTLEDYVGQTPLVRLQRLNAGRS-----TVLVKLEGNNPAGSVKDRPALNMIVQAEARG 58 Query: 63 QIKPGDTLIEATSGNTGIALAMVAALRGYKMVLLMPENLSEERRQSMAAYGAKIVLTPKS 122 ++PGDT+IEATSGNTGIALAM AA++GYKM+L+MP+N ++ER+ SM AYGA+++L K Sbjct: 59 SLQPGDTIIEATSGNTGIALAMAAAIKGYKMILIMPDNATQERKDSMRAYGAELILVSKE 118 Query: 123 GGMEYARDLAEQMQKEGQGLILDQFANGDNPQAHYETTGPELWRDTEGRITHFVSAMGTT 182 GME ARDLA QMQ+EG+G +LDQF N DNP AHY +TGPE+W+ ++G+ITHFVS+MGTT Sbjct: 119 QGMEGARDLALQMQQEGKGKVLDQFNNLDNPDAHYRSTGPEIWQQSQGKITHFVSSMGTT 178 Query: 183 GTIMGVSRYLKEQNPGIQIIGAQPEEGSSIPGIRKWPEAYLPKIYEKPRVDQIESVSQAA 242 GTIMGVSRYLK+QNP IQIIG QP EGS+IPGIR+WP+AYLP I++ RVDQ+ V+Q Sbjct: 179 GTIMGVSRYLKQQNPQIQIIGLQPSEGSAIPGIRRWPQAYLPGIFDAARVDQVLDVTQTD 238 Query: 243 AENMARKLAMVEGLFCGISAAGACEVALRISQQVENATIVFVVCDRGDRYLSTGVF 298 AE AR LA EG+ G+S+ GA AL+I+QQ + +V +VCDRGDRYLS+G+F Sbjct: 239 AEQTARALAREEGICAGVSSGGAVFAALQIAQQNPGSVVVAIVCDRGDRYLSSGLF 294 >STM2440 [E] COG0031 Cysteine synthase Sty Salmonella typhimurium LT2 Length = 303 Score = 387 bits (994), Expect = e-107, Method: Compositional matrix adjust. Identities = 186/294 (63%), Positives = 229/294 (77%), Gaps = 4/294 (1%) Query: 5 TIEDTIGNTPLIQLQRLGGEEARRRNNIILGKMEGNNPAGSVKDRAALSMIRRAEERGQI 64 T+E TIGNTPL++LQRLG + I K+EGNNPAGSVKDRAALSMI AE+RG+I Sbjct: 3 TLEQTIGNTPLVKLQRLGPDNGSE----IWVKLEGNNPAGSVKDRAALSMIVEAEKRGEI 58 Query: 65 KPGDTLIEATSGNTGIALAMVAALRGYKMVLLMPENLSEERRQSMAAYGAKIVLTPKSGG 124 KPGD LIEATSGNTGIALAM+AAL+GY+M LLMP+N+S+ERR +M AYGA+++L K G Sbjct: 59 KPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQG 118 Query: 125 MEYARDLAEQMQKEGQGLILDQFANGDNPQAHYETTGPELWRDTEGRITHFVSAMGTTGT 184 ME ARDLA M + G+G +LDQF N DNP AHY TTGPE+WR T GRITHFVS+MGTTGT Sbjct: 119 MEGARDLALAMSERGEGKLLDQFNNPDNPYAHYTTTGPEIWRQTSGRITHFVSSMGTTGT 178 Query: 185 IMGVSRYLKEQNPGIQIIGAQPEEGSSIPGIRKWPEAYLPKIYEKPRVDQIESVSQAAAE 244 I GVSR+L+EQ + I+G QPEEGSSIPGIR+WP Y+P I+ VD++ + Q AE Sbjct: 179 ITGVSRFLREQEKPVTIVGLQPEEGSSIPGIRRWPAEYMPGIFNASLVDEVLDIHQNDAE 238 Query: 245 NMARKLAMVEGLFCGISAAGACEVALRISQQVENATIVFVVCDRGDRYLSTGVF 298 N R+LA+ EG+FCG+S+ GA ALR+++ A +V ++CDRGDRYLSTGVF Sbjct: 239 NTMRELAVREGIFCGVSSGGAVAGALRVARATPGAIVVAIICDRGDRYLSTGVF 292 >cysM [E] COG0031 Cysteine synthase Eco Escherichia coli K12 Length = 303 Score = 384 bits (987), Expect = e-106, Method: Compositional matrix adjust. Identities = 183/294 (62%), Positives = 231/294 (78%), Gaps = 4/294 (1%) Query: 5 TIEDTIGNTPLIQLQRLGGEEARRRNNIILGKMEGNNPAGSVKDRAALSMIRRAEERGQI 64 T+E TIGNTPL++LQR+G + + K+EGNNPAGSVKDRAALSMI AE+RG+I Sbjct: 3 TLEQTIGNTPLVKLQRMGPDNGSE----VWLKLEGNNPAGSVKDRAALSMIVEAEKRGEI 58 Query: 65 KPGDTLIEATSGNTGIALAMVAALRGYKMVLLMPENLSEERRQSMAAYGAKIVLTPKSGG 124 KPGD LIEATSGNTGIALAM+AAL+GY+M LLMP+N+S+ERR +M AYGA+++L K G Sbjct: 59 KPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQG 118 Query: 125 MEYARDLAEQMQKEGQGLILDQFANGDNPQAHYETTGPELWRDTEGRITHFVSAMGTTGT 184 ME ARDLA +M G+G +LDQF N DNP AHY TTGPE+W+ T GRITHFVS+MGTTGT Sbjct: 119 MEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSMGTTGT 178 Query: 185 IMGVSRYLKEQNPGIQIIGAQPEEGSSIPGIRKWPEAYLPKIYEKPRVDQIESVSQAAAE 244 I GVSR+++EQ+ + I+G QPEEGSSIPGIR+WP YLP I+ VD++ + Q AE Sbjct: 179 ITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLPGIFNASLVDEVLDIHQRDAE 238 Query: 245 NMARKLAMVEGLFCGISAAGACEVALRISQQVENATIVFVVCDRGDRYLSTGVF 298 N R+LA+ EG+FCG+S+ GA ALR+++ +A +V ++CDRGDRYLSTGVF Sbjct: 239 NTMRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVVAIICDRGDRYLSTGVF 292