BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0229.p (265 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl117 [IQR] COG1028 Dehydrogenases with different specificities... 265 6e-71 SMc00264 [IQR] COG1028 Dehydrogenases with different specificiti... 265 6e-71 lin2278 [IQR] COG1028 Dehydrogenases with different specificitie... 164 1e-40 RSc2351 [IQR] COG1028 Dehydrogenases with different specificitie... 160 3e-39 TM0325 [IQR] COG1028 Dehydrogenases with different specificities... 157 2e-38 >AGl117 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 271 Score = 265 bits (678), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 134/259 (51%), Positives = 178/259 (68%), Gaps = 1/259 (0%) Query: 1 MSERMKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEG 60 ++ R+ GK A+V GAGSSGPG+GNGKAAAV +AREGAR+ VD +AA+ETA IIR EG Sbjct: 5 LTGRLTGKRALVFGAGSSGPGFGNGKAAAVQFAREGARVACVDLCADAAEETAEIIRGEG 64 Query: 61 GECTAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPVETSEESWDRVIAVN 120 E A ADV + +G ID+LHNNVG+ GGPVE EES+ + +N Sbjct: 65 LEAIAAAADVTELQSVSATVDRTCEAFGGIDILHNNVGVTHMGGPVELDEESFRASVDLN 124 Query: 121 QTSIFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYLAYSAAKAALISMTSNIAVEY 180 S++ T K VLP M A+ GAIVNI S+A +RW G+PY AY A KAA+ T +A++Y Sbjct: 125 IGSVYRTSKAVLPVMLAQGGGAIVNISSLASIRWTGYPYFAYYAMKAAVNQATVALAMQY 184 Query: 181 AAQGIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKISPTGKMGDAWDTAYAALF 240 A QGIRANC+LPG+++TPLI + ++ Y +V+ M+A R+ P G+MGDA+D A AA+F Sbjct: 185 ARQGIRANCILPGMIDTPLIYKQISNQY-ASVEEMVAARNAAVPVGRMGDAFDIARAAVF 243 Query: 241 LASDEARYVNGTSIIVDGG 259 LASDEA+++ G + VDGG Sbjct: 244 LASDEAKFITGVCLPVDGG 262 >SMc00264 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 267 Score = 265 bits (678), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 143/259 (55%), Positives = 183/259 (70%), Gaps = 1/259 (0%) Query: 1 MSERMKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEG 60 M++R+KGK AIV GAGSSGPGWGNGKAAAVLYAREGAR+ VD +EAA+ETAAII SEG Sbjct: 1 MADRLKGKSAIVFGAGSSGPGWGNGKAAAVLYAREGARVACVDVDMEAAEETAAIIASEG 60 Query: 61 GECTAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPVETSEESWDRVIAVN 120 G+ A ADV + + AA +G I +LHNNVG+ GGPVE SE + + + +N Sbjct: 61 GQAVAIAADVTALASVEAAVAAARQAFGIISILHNNVGVTHMGGPVELSEAQFQQSVDLN 120 Query: 121 QTSIFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYLAYSAAKAALISMTSNIAVEY 180 S+F T K V+P+M GA+VNI S+AG+RW G+PY AY A KAA+ T IA +Y Sbjct: 121 LGSVFRTAKAVIPHMIEAGGGAVVNISSLAGIRWTGYPYFAYYATKAAVNQATVAIAAQY 180 Query: 181 AAQGIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKISPTGKMGDAWDTAYAALF 240 A GIRANCV+PGL++TPLI + ++ Y + + M+A R+ P G+MGDAWD A AALF Sbjct: 181 APHGIRANCVVPGLIDTPLIYKQISSQY-ASAEEMVAARNAALPGGRMGDAWDVANAALF 239 Query: 241 LASDEARYVNGTSIIVDGG 259 LASDEA+++ G + VDGG Sbjct: 240 LASDEAKFITGVCLPVDGG 258 >lin2278 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Lin Listeria innocua Length = 253 Score = 164 bits (416), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 101/260 (38%), Positives = 141/260 (54%), Gaps = 11/260 (4%) Query: 4 RMKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEGGEC 63 ++ GKVA+V GA S G G+ AVL+A+EGA++ D +EAAQ+T A+I E G Sbjct: 3 KLAGKVAVVTGAAS-----GMGQQIAVLFAKEGAKVVVADLNLEAAQKTVALIEDEQGTA 57 Query: 64 TAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPV-ETSEESWDRVIAVNQT 122 A A+V DDI M TYG +D+L NN GI++ P E ++E WD+V A+N T Sbjct: 58 LAVVANVTKQDDIESMINKATETYGTLDILVNNAGIMDNFVPAGELTDELWDKVFAINTT 117 Query: 123 SIFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYLAYSAAKAALISMTSNIAVEYAA 182 + + L + G IVNI S GL + AY+A+K A++ T N+ +YA Sbjct: 118 GVMRATRKALHIFEEKGQGVIVNIASAGGL-FGSRAGAAYTASKHAVVGFTKNVGFQYAN 176 Query: 183 QGIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKISPTGKMGDAWDTAYAALFLA 242 + IR N + PG +NT + G +MI + + GDA + A ALFLA Sbjct: 177 KNIRCNAIAPGAVNTNIGTTIYAPDQFGQERAMIG----MGTNPRAGDASEIAKVALFLA 232 Query: 243 SDEARYVNGTSIIVDGGLTA 262 SD+A +VNGT I D G TA Sbjct: 233 SDDASFVNGTVITADAGWTA 252 >RSc2351 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 252 Score = 160 bits (404), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 102/266 (38%), Positives = 147/266 (55%), Gaps = 19/266 (7%) Query: 4 RMKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEGGEC 63 R+ GK+AIV GAGS G G+ A +AREGARI D IEA + A+ IR GG+ Sbjct: 2 RLAGKIAIVTGAGS-----GFGEGIATTFAREGARIIVNDLNIEAGERVASAIRVAGGDA 56 Query: 64 TAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPV-ETSEESWDRVIAVNQT 122 ADV+ + + + AA + YG +D++ NN G PV E +E +DRV AVN Sbjct: 57 HFVHADVSDGEAVASLLAATLERYGDLDIVVNNAGTTHRNKPVLEITEHEFDRVFAVNVK 116 Query: 123 SIFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYLA-YSAAKAALISMTSNIAVEYA 181 S++ T +H++P+ AR G VN+ S AG+R P L Y+A+K A+I+ + +A E Sbjct: 117 SLYWTARHMVPHFRARGGGVFVNVASTAGVR--PRPGLVWYNASKGAVITASKAMAAELG 174 Query: 182 AQGIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKIS--PTGKMGDAWDTAYAAL 239 IR NCV P + T L+ + + G D+ + ++ P G++ D A A L Sbjct: 175 PDRIRVNCVNPVMGATGLLEQ-----FMGMPDTPENRARFLASIPMGRLSTPQDVANACL 229 Query: 240 FLASDEARYVNGTSIIVDGGLTARCV 265 +LASDEA ++ G + VDGG RCV Sbjct: 230 YLASDEAEFITGACLEVDGG---RCV 252 >TM0325 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 251 Score = 157 bits (397), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 98/259 (37%), Positives = 144/259 (55%), Gaps = 12/259 (4%) Query: 5 MKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEGGECT 64 +GKV ++ GAGS G GK AAV++A GA++ D E +ET +I+S GGE Sbjct: 3 FQGKVVLITGAGS-----GIGKKAAVMFAERGAKVAINDISEEKGKETVELIKSMGGEAA 57 Query: 65 AFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPVETSEESWDRVIAVNQTSI 124 DVA D ++ + T+G++D+L NN GIV G ETSEE +D+ +AVN Sbjct: 58 FIFGDVAK--DAEQIVKKTVETFGRLDILVNNAGIVPYGNIEETSEEDFDKTMAVNVKGP 115 Query: 125 FLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFP-YLAYSAAKAALISMTSNIAVEYAAQ 183 FL K+ + M + G IVN+ S AGL IG P YS +KAAL+ +T ++AV+Y Sbjct: 116 FLLSKYAVEQMKKQGGGVIVNVSSEAGL--IGIPRRCVYSVSKAALLGLTRSLAVDYVDY 173 Query: 184 GIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKISPTGKMGDAWDTAYAALFLAS 243 GIR N V PG + + + + N + ++ + P ++G + A+A LF A Sbjct: 174 GIRVNAVCPGTTQSEGLMARVKASP--NPEELLKKMTSRIPMKRLGKEEEIAFAILFAAC 231 Query: 244 DEARYVNGTSIIVDGGLTA 262 DEA ++ G+ I +DGG TA Sbjct: 232 DEAGFMTGSIINIDGGSTA 250