BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0044.p (393 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 447 e-125 AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 419 e-117 SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carbox... 413 e-115 AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 411 e-114 PA2922 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 409 e-114 >PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Pae Pseudomonas aeruginosa Length = 406 Score = 447 bits (1150), Expect = e-125, Method: Compositional matrix adjust. Identities = 219/385 (56%), Positives = 276/385 (71%), Gaps = 2/385 (0%) Query: 8 IADLDDVKDELVGIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGHGLVATLR 67 +A L++V D+L +R+ IH HPEL +EE TAALVA L WGY V IG G+V LR Sbjct: 8 VAWLEEVADDLRSLRQDIHAHPELGFEERRTAALVAECLRGWGYEVHEGIGRTGVVGVLR 67 Query: 68 SGSSTRSIGVRADMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQLSRTRNF 127 G TR +G+RADMDALPI E TGL Y+S G+MHACGHDGHTAMLLGAA L+ TR F Sbjct: 68 QGDGTRRLGLRADMDALPIVEATGLGYSSCHGGRMHACGHDGHTAMLLGAARYLAATRRF 127 Query: 128 DGTVNLIFQPAEEAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMFRAGPFMA 187 DGT+ LIFQPAEE GA M+ DGL ERFPCDA+FGMHN PG+E G FRAGP MA Sbjct: 128 DGTLVLIFQPAEEG--QGGAEAMLADGLLERFPCDALFGMHNMPGLEAGHLGFRAGPMMA 185 Query: 188 ACDTVKIKILGKGSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVTVGSLHAG 247 + D + + + G G H + PH S+DPL+AASS VMALQ+VV+RN+DP +AAVVTVG+L AG Sbjct: 186 SQDLLSVTLEGVGGHGSMPHLSVDPLLAASSAVMALQSVVARNVDPQKAAVVTVGALQAG 245 Query: 248 HAANVIPEHATMELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRGYPVLVNS 307 AANVIP+ A + LS+R+ +VRE + QR+R +++ YG +A +E+ YPVLVNS Sbjct: 246 EAANVIPQRAVLRLSLRALDGQVREQVLQRVRQIIELQAASYGCQASIEHYPAYPVLVNS 305 Query: 308 EAETEFAKQIAVELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGNGAGKPMLHNAH 367 ETEFA+Q+ VEL GA+++ P + GSEDFA+ LQ+ PG +L +GNG G+PM+HN Sbjct: 306 VEETEFARQVGVELAGAEQVDGDTPKLMGSEDFAWMLQRCPGSYLFIGNGRGRPMVHNPA 365 Query: 368 YDFSDDNVTVGAAYWTRLVERYLQA 392 YDF+DD + GAAYW L E +L A Sbjct: 366 YDFNDDILVRGAAYWGALAETWLAA 390 >AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 422 Score = 419 bits (1076), Expect = e-117, Method: Compositional matrix adjust. Identities = 201/375 (53%), Positives = 267/375 (71%), Gaps = 3/375 (0%) Query: 16 DELVGIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGHGLVATLRSGSSTRSI 75 +E + IR +H+HPELS +E TAAL+A L S+GY V +GGHG+VATL+ G + + Sbjct: 50 EESIAIRHDLHRHPELSLKETRTAALIARYLLSFGYEVTEGVGGHGVVATLKRGHGEKRL 109 Query: 76 GVRADMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQLSRTRNFDGTVNLIF 135 G+RAD+DALPI+E TGL+YASE G MHACGHDGHT +LL AA L+ T NF GT+ LIF Sbjct: 110 GIRADIDALPIHEETGLAYASETSGIMHACGHDGHTTVLLAAARYLAETSNFSGTLTLIF 169 Query: 136 QPAEEAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMFRAGPFMAACDTVKIK 195 QPAEE G +GA MI+D LFERFP DA+FG+HN PGVETG F F GP MA+ D ++ Sbjct: 170 QPAEEIG--AGARAMIKDRLFERFPVDAVFGLHNWPGVETGQFGFVEGPAMASVDKASVR 227 Query: 196 ILGKGSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVTVGSLHAGHAANVIPE 255 I+GKG H A PH+++DP++A++S + ALQ++VSRNIDP E AV+TVGS+H G A+NVIPE Sbjct: 228 IIGKGGHGAAPHETVDPVLASASFITALQSIVSRNIDPREMAVLTVGSIHGGTASNVIPE 287 Query: 256 HATMELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRGYPVLVNSEAETEFAK 315 ++++ R+F VR+ LE+R+ AL ++ E +GA A+V Y RG+P ++N ET FA+ Sbjct: 288 SVELQITARAFSTAVRDRLEERLPALAKAQAESFGATADVSYSRGFPPVINHAPETAFAR 347 Query: 316 QIAVELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGNGAGKPMLHNAHYDFSDDNV 375 +A A +I +F P SEDFA+ LQ+RPG +L VGNG P LH+ HYDF+D + Sbjct: 348 DVARRHFPAAQIADAFQPRTASEDFAFMLQERPGSYLFVGNGESAP-LHSPHYDFNDAII 406 Query: 376 TVGAAYWTRLVERYL 390 A +W RL E YL Sbjct: 407 PPAARFWVRLAEAYL 421 >SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Sme Sinorhizobium meliloti Length = 389 Score = 413 bits (1061), Expect = e-115, Method: Compositional matrix adjust. Identities = 198/384 (51%), Positives = 265/384 (69%), Gaps = 4/384 (1%) Query: 9 ADLDDVKDELVGIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGHGLVATLRS 68 A +D+ ELV IRR +H HPEL EE T+A +A LE GY VA I G+V TLR+ Sbjct: 5 AAIDNAMPELVAIRRDLHAHPELGLEETRTSAFIARHLEELGYEVATGIAKTGVVGTLRN 64 Query: 69 GSSTRSIGVRADMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQLSRTRNFD 128 G+ +RSIG+RAD+DALPI E TG++YAS + G MHACGHDGHTAMLLGAA L+ RNFD Sbjct: 65 GTGSRSIGIRADIDALPIQEETGVAYASTKPGLMHACGHDGHTAMLLGAARALAERRNFD 124 Query: 129 GTVNLIFQPAEEAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMFRAGPFMAA 188 GT++LIFQPAEE GA M+ +GLF+RFPCDA+F +HN P + G F R GP MAA Sbjct: 125 GTIHLIFQPAEENA--GGAKIMVDEGLFDRFPCDAVFALHNEPNLPFGQFALREGPIMAA 182 Query: 189 CDTVKIKILGKGSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVTVGSLHAGH 248 D +I + G+G H A P + DP+V +S+VMALQT+V+RNI PM+ +VVTVG+ HAG Sbjct: 183 VDEARITVHGRGGHGAEPQATADPIVCGASIVMALQTIVARNIHPMDPSVVTVGAFHAGS 242 Query: 249 AANVIPEHATMELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRGYPVLVNSE 308 A+N+IPE A + + +RSF VR+ LE+RIR + ++ +G +A V+Y R Y +N + Sbjct: 243 ASNIIPERAEIVVGIRSFDPAVRDELERRIRMIAEAQASSFGMRATVDYERSYDATINHK 302 Query: 309 AETEFAKQIAVELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGN--GAGKPMLHNA 366 AET+F ++ A+ GADK++ P GSEDFAY L++RPG + +G+ + LH+ Sbjct: 303 AETDFLREAAIRFAGADKVVDLARPFMGSEDFAYMLKERPGSYFFLGSRVTGEEKSLHHP 362 Query: 367 HYDFSDDNVTVGAAYWTRLVERYL 390 YDF+DD + +GAA+WT L E YL Sbjct: 363 GYDFNDDLLPIGAAFWTELAEAYL 386 >AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 393 Score = 411 bits (1056), Expect = e-114, Method: Compositional matrix adjust. Identities = 200/395 (50%), Positives = 266/395 (67%), Gaps = 4/395 (1%) Query: 1 MSTEFCRIADLDDVKDELVGIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGH 60 M+ + IA + ++V IRRH+H+HPE+ E T+ +A +L GY V R + G Sbjct: 1 MTRQTANIASFANHMPDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGT 60 Query: 61 GLVATLRSGSSTRSIGVRADMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQ 120 G+VATLR+G STR++G+RAD+DALPI+E TG YAS QG MHACGHDGHTAMLLGAA Sbjct: 61 GIVATLRNGDSTRALGIRADIDALPIHEETGADYASTHQGVMHACGHDGHTAMLLGAAKI 120 Query: 121 LSRTRNFDGTVNLIFQPAEEAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMF 180 ++ RNFDGT++LIFQPAEE GA MI DGLFERFPCDA+F +HN PG+ G F+ Sbjct: 121 IAERRNFDGTLHLIFQPAEEN--FGGARIMIEDGLFERFPCDAVFALHNDPGLPFGQFVL 178 Query: 181 RAGPFMAACDTVKIKILGKGSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVT 240 R GP +AA D KI + G G H A P + DP+VA +S++MALQTVVSRNI P +AVVT Sbjct: 179 RDGPILAAVDECKITVRGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVT 238 Query: 241 VGSLHAGHAANVIPEHATMELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRG 300 VG+ HAG A+NVIPE A M L++RSF VR+ LE+RIRA+ + YG ++Y RG Sbjct: 239 VGAFHAGMASNVIPETAEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERG 298 Query: 301 YPVLVNSEAETEFAKQIAVELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGNGA-- 358 Y VN +AET++ +A G +K++ P G+EDFAY L++RPGC+ +G Sbjct: 299 YNATVNHKAETDYVADLARRFAGPEKVLEMKRPSMGAEDFAYMLEKRPGCYFFLGTARTD 358 Query: 359 GKPMLHNAHYDFSDDNVTVGAAYWTRLVERYLQAQ 393 P LH+ +DF+D+ + +G +W L E YL+A+ Sbjct: 359 NDPPLHHPKFDFNDEILPIGTTFWVDLAEDYLKAR 393 >PA2922 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Pae Pseudomonas aeruginosa Length = 389 Score = 409 bits (1050), Expect = e-114, Method: Compositional matrix adjust. Identities = 203/390 (52%), Positives = 269/390 (68%), Gaps = 5/390 (1%) Query: 3 TEFCRIADLDDVKDELVGIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGHGL 62 TE + + +++E+V +RRHIH HPEL +EE TAALVA LE+WGY V+R +G G+ Sbjct: 2 TEPAVLPAIAALREEMVTLRRHIHAHPELGFEERRTAALVAECLEAWGYQVSRGVGRTGV 61 Query: 63 VATLRSGSSTRSIGVRADMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQLS 122 V TL G ++G+RADMDALPI E TGL YAS+ G MHACGHDGHTAMLL AA L Sbjct: 62 VGTLCRGEGP-ALGLRADMDALPIQEATGLPYASQVDGVMHACGHDGHTAMLLAAARHLV 120 Query: 123 RTRNFDGTVNLIFQPAEEAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMFRA 182 + ++ GT+ LIFQPAEE GA M+ DGL ERFPCDAIF MHN PG G F Sbjct: 121 ESPHWRGTLQLIFQPAEEG--LGGARAMLDDGLLERFPCDAIFAMHNVPGYPVGHLGFHP 178 Query: 183 GPFMAACDTVKIKILGKGSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVTVG 242 GPFMA+ DTV I+++G G H A P +++DP+V S++V+ALQ++VSRN+DP + A+V+VG Sbjct: 179 GPFMASADTVVIRVIGSGGHGAVPQRTVDPVVVCSAIVLALQSIVSRNVDPQDTAIVSVG 238 Query: 243 SLHAGHAANVIPEHATMELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRGYP 302 ++HAG +NVIP A M LSVR+ E R LLE+RI L + +GA+AEV+Y +P Sbjct: 239 AIHAGTVSNVIPASAEMILSVRALTAETRALLERRIGELARGQAASFGARAEVDYRHCHP 298 Query: 303 VLVNSEAETEFAKQIAVELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGNGAGKP- 361 VLVN +T FA+++A + +G + +I P SEDFA+ L++ PG +L +GNG G+ Sbjct: 299 VLVNHPGQTAFAREVARDWLGEECLIDGLRPFTASEDFAFILERCPGSYLVIGNGQGESG 358 Query: 362 -MLHNAHYDFSDDNVTVGAAYWTRLVERYL 390 LHN YDF+DD + VGA+YW RL ER+L Sbjct: 359 CQLHNPGYDFNDDCLAVGASYWVRLAERFL 388