BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0028.p (256 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr5278 [IQR] COG1028 Dehydrogenases with different specificitie... 416 e-116 AGpA300 [IQR] COG1028 Dehydrogenases with different specificitie... 250 2e-66 BS_ykvO [IQR] COG1028 Dehydrogenases with different specificitie... 247 2e-65 mlr5280 [IQR] COG1028 Dehydrogenases with different specificitie... 235 8e-62 msl7784m [IQR] COG1028 Dehydrogenases with different specificiti... 231 1e-60 >mlr5278 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 244 Score = 416 bits (1070), Expect = e-116, Method: Compositional matrix adjust. Identities = 214/256 (83%), Positives = 226/256 (88%), Gaps = 12/256 (4%) Query: 1 MNRLHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGS 60 M RL+ KTAVITGGATGIG AAA RFIEEGAFVFI+GRRQEALDAA+ADLG NARAVKGS Sbjct: 1 MTRLNGKTAVITGGATGIGRAAATRFIEEGAFVFIYGRRQEALDAAVADLGANARAVKGS 60 Query: 61 ITDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTVQ 120 ++D DLDRLYAAVKAERGTLDIVFANAGAGGPLPLG++TAEHIDETFDTNVKGTIFTVQ Sbjct: 61 VSDLADLDRLYAAVKAERGTLDIVFANAGAGGPLPLGQITAEHIDETFDTNVKGTIFTVQ 120 Query: 121 KALPLMGQGGSIILTGSSAGTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIRVNVLSP 180 +ALPLMG GGSIILTGSSAGTTGAP F+ YSASKAAVRNLARTWAEDLKGTGIRVNVLSP Sbjct: 121 QALPLMGPGGSIILTGSSAGTTGAPGFTAYSASKAAVRNLARTWAEDLKGTGIRVNVLSP 180 Query: 181 GPTATELAKAALGEEGMKVFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASEVAVDXD 240 G TATELAK ALGEEG K + AM PLQRMADPAE+GA AAFLAS DSSFMTASEVAVD Sbjct: 181 GATATELAKEALGEEGQKAYGAMTPLQRMADPAEIGAVAAFLASSDSSFMTASEVAVD-- 238 Query: 241 SSFMTASEVAGGLAQI 256 GGLAQ+ Sbjct: 239 ----------GGLAQL 244 >AGpA300 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 251 Score = 250 bits (638), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 136/243 (55%), Positives = 169/243 (69%), Gaps = 6/243 (2%) Query: 2 NRLHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGSI 61 NRL K AV+TG +GIGLA AK F EGA V+I GRR++ALD A+A++G A V+ Sbjct: 3 NRLQNKIAVVTGATSGIGLATAKLFAVEGARVYITGRRKDALDKAVAEIGHGAVGVQADS 62 Query: 62 TDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTVQK 121 ++LD+L+A VKAE+G LD + NAG G LPLG++T E ID TF NVK IFTVQK Sbjct: 63 ASNENLDKLFAQVKAEQGRLDALVVNAGGGSMLPLGQITEEQIDHTFGRNVKAVIFTVQK 122 Query: 122 ALPLMGQGGSIILTGSSAGTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIRVNVLSPG 181 ALPLMG+G SI+LTGS+AGT G AFSVYSASKAAVRNLAR+WA DLKGTGIRVNV+SPG Sbjct: 123 ALPLMGKGASIVLTGSTAGTEGTAAFSVYSASKAAVRNLARSWALDLKGTGIRVNVVSPG 182 Query: 182 PTAT----ELAKAALGEEG--MKVFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASEV 235 T T ELA ++ + A+ PL R+ + E+ A FLAS D+SF+ +E+ Sbjct: 183 ATRTPGLVELAGDDKEQQQGLLDYLASRIPLGRVGESEEIAKATLFLASDDASFVNGAEL 242 Query: 236 AVD 238 D Sbjct: 243 FAD 245 >BS_ykvO [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 248 Score = 247 bits (631), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 135/261 (51%), Positives = 173/261 (66%), Gaps = 18/261 (6%) Query: 1 MNRLHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGS 60 M + K A++TGG +GIGLA A++F+ EGA+V+I GRRQ LD A+ +G N V+G Sbjct: 1 MGKFEGKIALVTGGTSGIGLATAQKFVNEGAYVYITGRRQNELDKAVNQIGKNVTGVQGD 60 Query: 61 ITDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTVQ 120 I+ +DLD+LY +K E+G LDI+FANAG G LPLG++T E +D TFD NVKGTIFTVQ Sbjct: 61 ISKLEDLDKLYDIIKQEKGKLDILFANAGIGNFLPLGEITEEQVDRTFDINVKGTIFTVQ 120 Query: 121 KALPLM-GQGGSIILTGSSAGTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIRVNVLS 179 KAL L + GSII+TGS+AG+ G PAFSVY ASKAA+R L R W DLKGT IRVNV+S Sbjct: 121 KALSLFPDKVGSIIVTGSTAGSIGNPAFSVYGASKAALRALVRNWILDLKGTEIRVNVVS 180 Query: 180 PG----PTATELAKAALGEEGMKVFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASEV 235 PG P EL AL EE ++ P ++ P E+ A +FLAS +SS++T E+ Sbjct: 181 PGGILTPAYDELFGDAL-EEVLENSRNTVPAGKVGTPEEVANAVSFLASDESSYLTGVEL 239 Query: 236 AVDXDSSFMTASEVAGGLAQI 256 VD GGLAQ+ Sbjct: 240 FVD------------GGLAQV 248 >mlr5280 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 250 Score = 235 bits (599), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 129/262 (49%), Positives = 161/262 (61%), Gaps = 18/262 (6%) Query: 1 MNRLHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGS 60 M +L K AVITGG++GIGLA AKRF+EEGA V I GRR++ L A A + N V G Sbjct: 1 MKKLEGKIAVITGGSSGIGLATAKRFVEEGAHVVITGRREKELKEAAAFIMRNVTTVVGD 60 Query: 61 ITDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTVQ 120 ++ +DLDRLYA VK + G +D++FANAGAG PL T H D+TFD NVKG FTVQ Sbjct: 61 VSLLEDLDRLYAVVKEKHGHIDVLFANAGAGTIAPLAAATEAHFDQTFDVNVKGLFFTVQ 120 Query: 121 KALPLMGQGGSIILTGSSAGTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIRVNVLSP 180 KALPL GGSIIL S + G P FS Y+ASKAAVRN +R W +LK IRVN +SP Sbjct: 121 KALPLFKDGGSIILNSSVSNVLGLPGFSTYAASKAAVRNFSRAWTLELKDRKIRVNTMSP 180 Query: 181 GPTATELAKAALG------EEGMKVFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASE 234 G T + G E+ + FA+ P+ R P E+ AA FLAS DSS++T + Sbjct: 181 GAIETPALETTTGLTPEQAEQAVAQFASQIPMGRRGKPEEIAAAVTFLASDDSSYVTGVD 240 Query: 235 VAVDXDSSFMTASEVAGGLAQI 256 +AVD GG+AQ+ Sbjct: 241 LAVD------------GGMAQV 250 >msl7784m [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 251 Score = 231 bits (589), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 134/243 (55%), Positives = 168/243 (69%), Gaps = 6/243 (2%) Query: 2 NRLHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGSI 61 N+L K AV+TGG++GIGL AKRF EGA V+I GRRQ +D A+A +G +A ++ Sbjct: 3 NKLEGKIAVVTGGSSGIGLGIAKRFAAEGAHVYITGRRQAEIDKAVAAIGKHATGIQADS 62 Query: 62 TDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTVQK 121 T DLDRL VKA G +D++ NAG G LPLG++T +H DETFD NV +FTVQK Sbjct: 63 TSFTDLDRLTRRVKARSGRIDVLSVNAGGGSMLPLGQITEQHFDETFDRNVXAVLFTVQK 122 Query: 122 ALPLMGQGGSIILTGSSAGTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIRVNVLSPG 181 AL L+ G S+ILTGS+AG+TGA AFSVY ASKAA+RN AR+W DLK IRVN+LSPG Sbjct: 123 ALTLLADGASVILTGSTAGSTGAAAFSVYGASKAALRNFARSWIIDLKERQIRVNILSPG 182 Query: 182 PTAT----ELA-KAALGEEGMKVFAAMN-PLQRMADPAELGAAAAFLASQDSSFMTASEV 235 PT T ELA A ++G+ F A P+ R+ DP E+G AA FLAS DSSF+T +E+ Sbjct: 183 PTRTTGLAELAGPEAEQQKGLLDFLATQVPMGRLGDPDEIGKAAVFLASDDSSFVTGAEL 242 Query: 236 AVD 238 VD Sbjct: 243 FVD 245