BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0007.p (222 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1023 [IQR] COG1028 Dehydrogenases with different specificities... 335 6e-92 Rv0148 [IQR] COG1028 Dehydrogenases with different specificities... 160 2e-39 MT0156 [IQR] COG1028 Dehydrogenases with different specificities... 160 2e-39 PA3427 [IQR] COG1028 Dehydrogenases with different specificities... 146 3e-35 YKR009c_1 [IQR] COG1028 Dehydrogenases with different specificit... 140 2e-33 >PA1023 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 305 Score = 335 bits (858), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 154/222 (69%), Positives = 186/222 (83%) Query: 1 LFEQAIAAFGRVDGVVNNAGILRDRIFHKMSEEEWDAVLKVHLYGAYYMSRACAAHFKEQ 60 + E A+ FGR+D VVNNAGILRD IFHKMS ++W +V+ VHL G++++SRA A F+ Q Sbjct: 84 IVECAMDGFGRLDIVVNNAGILRDAIFHKMSADDWLSVINVHLNGSFFVSRAAAERFRTQ 143 Query: 61 NSGSMVHMTSTSGLIGNFGQANYSAAKLGVTALSKSIALDMQKFNVRSNCIAPFAWSRMV 120 +G+ VHMTSTSGLIGNFGQANYSAAKLG+ ALSKSIALDMQ++NVRSNCIAPFAWSRM Sbjct: 144 QAGAFVHMTSTSGLIGNFGQANYSAAKLGIVALSKSIALDMQRYNVRSNCIAPFAWSRMT 203 Query: 121 SSVPTDTPEQQARVDKIKQMTPAKIAPLAVYLASERAAQVNGQVFTVRNNEIFLMSQPRP 180 S+P +TPEQ+ARVDK+++MTP K APLAVYLAS+ A +V GQVF R++E+FLMSQ RP Sbjct: 204 DSIPAETPEQKARVDKLQRMTPEKNAPLAVYLASDAAREVTGQVFAARHHELFLMSQSRP 263 Query: 181 VRSVHRSEGWTPESIAEHGMPALAGSFFPLDRSGDIFTWDPV 222 +RSVH GWTPESIAEHGMPAL GSF L RS D+F+WDP+ Sbjct: 264 LRSVHSEHGWTPESIAEHGMPALRGSFMDLARSPDVFSWDPI 305 >Rv0148 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mtu Mycobacterium tuberculosis H37Rv Length = 286 Score = 160 bits (405), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 82/172 (47%), Positives = 111/172 (64%), Gaps = 9/172 (5%) Query: 1 LFEQAIAAFGRVDGVVNNAGILRDRIFHKMSEEEWDAVLKVHLYGAYYMSRACAAHFKEQ 60 + + A+ FG V GVV+NAGILRD FHKMS E WDAVLKVHLYG Y++ RA HF+EQ Sbjct: 82 IIKTALDEFGAVHGVVSNAGILRDGTFHKMSFENWDAVLKVHLYGGYHVLRAAWPHFREQ 141 Query: 61 NSGSMVHMTSTSGLIGNFGQANYSAAKLGVTALSKSIALDMQKFNVRSNCIAPFAWSRMV 120 + G +V TSTSGL GNFGQ NY AAKLG+ L ++AL+ K+N+ +N +AP A +RM Sbjct: 142 SYGRVVVATSTSGLFGNFGQTNYGAAKLGLVGLINTLALEGAKYNIHANALAPIAATRMT 201 Query: 121 SSVPTDTPEQQARVDKIKQMTPAKIAPLAVYLASERAAQVNGQVFTVRNNEI 172 + PE ++++TP +AP+ YL +E A N V+ V ++ Sbjct: 202 QDIL--PPEV------LEKLTPEFVAPVVAYLCTEECAD-NASVYVVGGGKV 244 >MT0156 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) MtC Mycobacterium tuberculosis CDC1551 Length = 293 Score = 160 bits (404), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 82/172 (47%), Positives = 111/172 (64%), Gaps = 9/172 (5%) Query: 1 LFEQAIAAFGRVDGVVNNAGILRDRIFHKMSEEEWDAVLKVHLYGAYYMSRACAAHFKEQ 60 + + A+ FG V GVV+NAGILRD FHKMS E WDAVLKVHLYG Y++ RA HF+EQ Sbjct: 89 IIKTALDEFGAVHGVVSNAGILRDGTFHKMSFENWDAVLKVHLYGGYHVLRAAWPHFREQ 148 Query: 61 NSGSMVHMTSTSGLIGNFGQANYSAAKLGVTALSKSIALDMQKFNVRSNCIAPFAWSRMV 120 + G +V TSTSGL GNFGQ NY AAKLG+ L ++AL+ K+N+ +N +AP A +RM Sbjct: 149 SYGRVVVATSTSGLFGNFGQTNYGAAKLGLVGLINTLALEGAKYNIHANALAPIAATRMT 208 Query: 121 SSVPTDTPEQQARVDKIKQMTPAKIAPLAVYLASERAAQVNGQVFTVRNNEI 172 + PE ++++TP +AP+ YL +E A N V+ V ++ Sbjct: 209 QDIL--PPEV------LEKLTPEFVAPVVAYLCTEECAD-NASVYVVGGGKV 251 >PA3427 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 303 Score = 146 bits (369), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 74/162 (45%), Positives = 103/162 (63%), Gaps = 8/162 (4%) Query: 1 LFEQAIAAFGRVDGVVNNAGILRDRIFHKMSEEEWDAVLKVHLYGAYYMSRACAAHFKEQ 60 + E A+ AFGRVD VVNNAGILRD+ FHKM + +WD V +VH+ GAY ++RA H +EQ Sbjct: 82 IVENALDAFGRVDVVVNNAGILRDKTFHKMEDADWDLVYQVHVEGAYKVTRAAWPHLREQ 141 Query: 61 NSGSMVHMTSTSGLIGNFGQANYSAAKLGVTALSKSIALDMQKFNVRSNCIAPFAWSRMV 120 G +V +STSG+ GNFGQ+NY AKLG+ L++++AL+ +K N+ N IAP +RM Sbjct: 142 AYGRVVFTSSTSGIYGNFGQSNYGMAKLGLYGLTRTLALEGRKNNILVNAIAPTGGTRMT 201 Query: 121 SSVPTDTPEQQARVDKIKQMTPAKIAPLAVYLASERAAQVNG 162 + P Q +Q+ P ++PL VYL S +G Sbjct: 202 EGL---IPPQ-----VFEQLKPELVSPLVVYLGSSACEDTSG 235 >YKR009c_1 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sce Saccharomyces cerevisiae Length = 622 Score = 140 bits (354), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 74/167 (44%), Positives = 106/167 (63%), Gaps = 9/167 (5%) Query: 1 LFEQAIAAFGRVDGVVNNAGILRDRIFHKMSEEEWDAVLKVHLYGAYYMSRACAAHFKEQ 60 + E AI FGRVD ++NNAGILRD F KM+E E+ +V+ VHL G Y +SRA + + Q Sbjct: 83 IIETAIKEFGRVDVLINNAGILRDVSFAKMTEREFASVVDVHLTGGYKLSRAAWPYMRSQ 142 Query: 61 NSGSMVHMTSTSGLIGNFGQANYSAAKLGVTALSKSIALDMQKFNVRSNCIAPFAWSRMV 120 G +++ S +GL GNFGQANYSAAK+G+ L++++A + K+N+ N IAP A SRM Sbjct: 143 KFGRIINTASPAGLFGNFGQANYSAAKMGLVGLAETLAKEGAKYNINVNSIAPLARSRMT 202 Query: 121 SSVPTDTPEQQARVDKIKQMTPAKIAPLAVYLASERAAQVNGQVFTV 167 +V P +KQ+ P KI PL +YL E + +V+ +F + Sbjct: 203 ENV---LPPHI-----LKQLGPEKIVPLVLYLTHE-STKVSNSIFEL 240 Score = 120 bits (300), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 83/203 (40%), Positives = 115/203 (56%), Gaps = 12/203 (5%) Query: 1 LFEQAIAAFGRVDGVVNNAGILRDRIFHKMSEEEWDAVLKVHLYGAYYMSRACAAHFKEQ 60 + + AI+ F RVD +VNNAGILRD+ F KM +EEW AVLKVHL+ + +S+A F +Q Sbjct: 387 IIQTAISKFQRVDILVNNAGILRDKSFLKMKDEEWFAVLKVHLFSTFSLSKAVWPIFTKQ 446 Query: 61 NSGSMVHMTSTSGLIGNFGQANYSAAKLGVTALSKSIALDMQKFNVRSNCIAPFAWSRMV 120 SG +++ TSTSG+ GNFGQANY+AAK + SK+IAL+ K + N IAP A + M Sbjct: 447 KSGFIINTTSTSGIYGNFGQANYAAAKAAILGFSKTIALEGAKRGIIVNVIAPHAETAMT 506 Query: 121 SSVPTDTPEQQARVDKIKQMTPAKIAPLAVYLASER-----AAQVNGQVFTVRNNEIFLM 175 ++ ++ E D ++++PL V LASE +V GQ+F V Sbjct: 507 KTIFSEK-ELSNHFD------ASQVSPLVVLLASEELQKYSGRRVIGQLFEVGGGWCGQT 559 Query: 176 SQPRPVRSVHRSEGWTPESIAEH 198 R V E PE I E+ Sbjct: 560 RWQRSSGYVSIKETIEPEEIKEN 582