BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0050.p (545 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2520 [P] COG3696 Putative silver efflux pump Pae Pseudomonas a... 639 0.0 RSp0493 [P] COG3696 Putative silver efflux pump Rso Ralstonia so... 622 e-178 CC2390 Non COG 544 e-154 CC2724 Non COG 513 e-145 aq_469 [P] COG3696 Putative silver efflux pump Aae Aquifex aeolicus 311 3e-84 >PA2520 [P] COG3696 Putative silver efflux pump Pae Pseudomonas aeruginosa Length = 1051 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/462 (69%), Positives = 380/462 (82%), Gaps = 1/462 (0%) Query: 1 MIVYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALM 60 M+VYLP+FALTGVEGKMF PMAFTVV ALLGAMILSVTF+PAAIALFI KV E+EN +M Sbjct: 460 MVVYLPIFALTGVEGKMFHPMAFTVVTALLGAMILSVTFVPAAIALFITGKVKEEENFVM 519 Query: 61 RAAKRAYAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQSLRIP 120 R A+ AY P L+ L ++ +V+ A I+L L+ +R+GSEF+PSL+EGD A+Q LR+P Sbjct: 520 RRARLAYEPALRWVLGHRALVVGGALGAILLTGLVASRMGSEFIPSLSEGDFAMQGLRVP 579 Query: 121 GTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDGYIMLKPQDQWP 180 GTSLTQSVEMQQ LER L FPEIERVFARTGTAE+ASD MPPN SD Y+MLKPQ QWP Sbjct: 580 GTSLTQSVEMQQTLERKLMGKFPEIERVFARTGTAEIASDLMPPNASDSYVMLKPQSQWP 639 Query: 181 KPRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMDVLND 240 P+K+R+ L+ +Q +PG+ YEFSQPIQLRFNELISGVRSDVAVKVFGDDM VLND Sbjct: 640 DPKKSREALLEELQAAALEVPGSVYEFSQPIQLRFNELISGVRSDVAVKVFGDDMQVLND 699 Query: 241 TAAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTARYGLNIGDVQDTVAMAIGGKE 300 TA KI+ L + GA+EVKVEQ+TGLP+LTV IDR+K AR+GLN+GD+QDTVA A+GG+ Sbjct: 700 TAEKISKVLQGIDGASEVKVEQTTGLPVLTVDIDRDKAARFGLNVGDIQDTVATALGGRN 759 Query: 301 AGALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPHND-GKLSYIPLGEVADFDIAPGP 359 AG LF+GDRRFDI++RLPE R DL A+ L IPLP N+ ++ +IPL +VA D++PGP Sbjct: 760 AGTLFEGDRRFDIVIRLPETLRADLPALSNLLIPLPPNNLARIDFIPLSDVARLDLSPGP 819 Query: 360 NQVSRENGKRRVVVSANVRGRDLGSFVDDAAAGIAAQVKIPTGYWIAWGGQFENLQSATQ 419 NQ+SRENGKRR+VVSANVRGRD+GSFV +A + VKIP GYW WGGQFE LQSA + Sbjct: 820 NQISRENGKRRIVVSANVRGRDIGSFVLEAQQKLQDGVKIPAGYWTTWGGQFEQLQSAAK 879 Query: 420 RLQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGV 461 RLQ+VVPVALLLVF LLFAMFNNVKDGLLVFTGIPFALTGGV Sbjct: 880 RLQVVVPVALLLVFTLLFAMFNNVKDGLLVFTGIPFALTGGV 921 >RSp0493 [P] COG3696 Putative silver efflux pump Rso Ralstonia solanacearum Length = 1064 Score = 622 bits (1603), Expect = e-178, Method: Compositional matrix adjust. Identities = 303/465 (65%), Positives = 368/465 (79%), Gaps = 7/465 (1%) Query: 1 MIVYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALM 60 M+VYLP+FAL+GVEGKMF PMA TVV AL AM+LS+TF+PAA+AL +G++V EKEN LM Sbjct: 460 MVVYLPIFALSGVEGKMFHPMAITVVLALAAAMVLSITFVPAAVALGMGERVAEKENRLM 519 Query: 61 RAAKRAYAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQSLRIP 120 A+R YAPLL+ +L VVL+ AA + + + RLG+EFVP+LNEGD +IQ++R+P Sbjct: 520 GWARRMYAPLLERALAAPAVVLTFAAVAVSVSLVAALRLGAEFVPNLNEGDFSIQAMRVP 579 Query: 121 GTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDGYIMLKPQDQWP 180 GT L QS+EMQ Q+ERTLK FPEI RVFARTGTAE+A+D MPP+ISDGYIML+P+ WP Sbjct: 580 GTGLAQSLEMQMQIERTLKAKFPEIARVFARTGTAEIATDVMPPSISDGYIMLRPEQDWP 639 Query: 181 K----PRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMD 236 R+ RD+L+A I+ TV LPGN+YEFSQPIQLRFNELISGVRSDVAVK+FGDDM Sbjct: 640 AGPDGTRRDRDQLLADIRATVETLPGNAYEFSQPIQLRFNELISGVRSDVAVKLFGDDMA 699 Query: 237 VLNDTAAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTARYGLNIGDVQDTVAMAI 296 VL A IA LG++ GA EVK+EQ+ GLP L + IDREK ARYGL IG +QDTV+ AI Sbjct: 700 VLEKQAQAIAAQLGRLAGAAEVKIEQTGGLPFLNIDIDREKAARYGLAIGAIQDTVSAAI 759 Query: 297 GGKEAGALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPHNDGKLSYIPLGEVADFDIA 356 GGK AG +FQGDRRF I+VRLPE R+D A+ RLP+ LP+ G Y+PL EVA Sbjct: 760 GGKGAGTVFQGDRRFGIVVRLPEAARSDPAALGRLPLALPNGQG---YVPLSEVATLHEV 816 Query: 357 PGPNQVSRENGKRRVVVSANVRGRDLGSFVDDAAAGIAAQVKIPTGYWIAWGGQFENLQS 416 GPNQVSRE+GKRR+VVSANVRGRD+ SFV +A + AQ+++P GYW+AWGGQFE LQS Sbjct: 817 TGPNQVSREDGKRRIVVSANVRGRDIASFVQEAQRALDAQIRLPAGYWMAWGGQFEQLQS 876 Query: 417 ATQRLQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGV 461 AT+RLQ+VVP+AL LVF LLF MF N+KDGLLVF+GIPFAL+GGV Sbjct: 877 ATERLQVVVPLALALVFTLLFMMFGNLKDGLLVFSGIPFALSGGV 921 >CC2390 Non COG Length = 1057 Score = 544 bits (1401), Expect = e-154, Method: Compositional matrix adjust. Identities = 262/461 (56%), Positives = 349/461 (75%), Gaps = 2/461 (0%) Query: 2 IVYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALMR 61 +VY+P+FALTGVEGKMF PMA TVV AL A++LS+TF+PAA+ALF+ KV+EKEN +MR Sbjct: 475 LVYVPIFALTGVEGKMFHPMAITVVIALTAALVLSLTFVPAAVALFVTGKVEEKENLIMR 534 Query: 62 AAKRAYAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQSLRIPG 121 A+R Y P L+ +LR ++ ++ A ++ + +R+GSEFVP+L+EGD+A+ +LRIPG Sbjct: 535 GARRLYEPALETALRLRVAFVAGAVLLVAIAAFAASRMGSEFVPNLDEGDIAMHALRIPG 594 Query: 122 TSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDGYIMLKPQDQWPK 181 TSL+Q++ MQ LE +K PE++RV ++ GTAEVA+DPMPP+++D +IMLK + WP Sbjct: 595 TSLSQAIAMQTALEAKVK-TLPEVDRVVSKIGTAEVATDPMPPSVADTFIMLKDRKDWPD 653 Query: 182 PRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMDVLNDT 241 PRK R +L+A ++ VA +PGN+YEF+QPIQ+RFNEL+SGVR+DVAVKVFGDD+D L Sbjct: 654 PRKPRAQLVAELEAVVAKVPGNNYEFTQPIQMRFNELLSGVRADVAVKVFGDDLDQLLAI 713 Query: 242 AAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTARYGLNIGDVQDTVAMAIGGKEA 301 +I G + V GA +V VEQ TGLP+L + DR AR GLN+ DVQ VA AIGG Sbjct: 714 GEQIGGVIEGVEGAQDVGVEQVTGLPVLQITPDRAALARLGLNVDDVQSVVATAIGGTVT 773 Query: 302 GALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPHN-DGKLSYIPLGEVADFDIAPGPN 360 G +F+GDRRFD++VRLPE R +D I RL IPLP D ++PL +VA ++ GPN Sbjct: 774 GQVFEGDRRFDVVVRLPEAVRGRIDDIGRLRIPLPGAIDQPRGFVPLQDVARIEMVIGPN 833 Query: 361 QVSRENGKRRVVVSANVRGRDLGSFVDDAAAGIAAQVKIPTGYWIAWGGQFENLQSATQR 420 Q+SRE+GKRR+VV+ANVRGRDLGSF+ + + AQV++PTGYWI +GG FE L SA QR Sbjct: 834 QISREDGKRRIVVTANVRGRDLGSFITEVQRKVGAQVELPTGYWITYGGAFEQLISAAQR 893 Query: 421 LQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGV 461 LQ+VVP ALLL+F LL+A+F +VKD +VF+G+P ALTGGV Sbjct: 894 LQLVVPAALLLIFGLLYALFRSVKDAAIVFSGVPLALTGGV 934 >CC2724 Non COG Length = 1080 Score = 513 bits (1320), Expect = e-145, Method: Compositional matrix adjust. Identities = 251/464 (54%), Positives = 334/464 (71%), Gaps = 3/464 (0%) Query: 1 MIVYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALM 60 ++VY P+ TGVEGK F PMA TV+ AL A +LS+TFIPA +AL I KV EKE AL+ Sbjct: 484 LLVYAPLLTFTGVEGKTFSPMAITVMLALAAAFVLSLTFIPAMVALLIRGKVAEKEVALV 543 Query: 61 RAAKRAYAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQSLRIP 120 R AK YAPLL+ ++ V++ A + V+ + T LG EF P L+E DLA+Q+LRIP Sbjct: 544 RWAKVGYAPLLRKAVARPWPVIAAATGLFVVAMITFTFLGREFTPQLDEKDLAVQALRIP 603 Query: 121 GTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDGYIMLKPQDQWP 180 TSL QS+ MQ+Q+ERT+ FPE+E V+++TGTAEVASDPMPPN SD +I+LK QD+WP Sbjct: 604 STSLEQSLRMQRQIERTIA-AFPEVEFVYSKTGTAEVASDPMPPNASDSFIILKAQDEWP 662 Query: 181 KPRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMDVLND 240 ++++ +L+ ++ + L GN YEFSQPIQ+RFNELI+GVR DVA+KV+GDD+ + Sbjct: 663 DSKQSKAQLVDRMERKLEGLTGNVYEFSQPIQMRFNELIAGVRGDVAIKVYGDDLAAMTA 722 Query: 241 TAAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTARYGLNIGDVQDTVAMAIGGKE 300 +A +IAG L V GA +VKVEQ++G P L V +DR+ R GL + +V DT+A+A+GG+ Sbjct: 723 SADQIAGVLRGVRGAADVKVEQTSGFPTLDVSLDRDAIGRLGLTVEEVADTLAVAMGGRA 782 Query: 301 AGALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPH--NDGKLSYIPLGEVADFDIAPG 358 +G +FQGDRRF+++VRLPE RNDLDAI LP+ LPH + G +PL +VA F + G Sbjct: 783 SGLVFQGDRRFEVVVRLPEATRNDLDAIGALPVMLPHGASAGPRLSVPLRDVARFSFSEG 842 Query: 359 PNQVSRENGKRRVVVSANVRGRDLGSFVDDAAAGIAAQVKIPTGYWIAWGGQFENLQSAT 418 NQVSRENGKRRVVV ANVRG DLGSFV +A A ++AQ+++P G W+ W GQFENL++A Sbjct: 843 LNQVSRENGKRRVVVQANVRGNDLGSFVSEAQAKVSAQIELPAGSWVEWSGQFENLKAAQ 902 Query: 419 QRLQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGVY 462 RL +VVP+ LL+F LL+ L VF+ IP AL GGV+ Sbjct: 903 SRLSLVVPLCFLLIFALLYMALGGFAPALAVFSAIPLALAGGVF 946 >aq_469 [P] COG3696 Putative silver efflux pump Aae Aquifex aeolicus Length = 1019 Score = 311 bits (797), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 175/461 (37%), Positives = 268/461 (58%), Gaps = 9/461 (1%) Query: 1 MIVYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALM 60 +IV+ P+F VEGK F+P+A T++ ALL ++ ++ TFIPA F+ E + LM Sbjct: 446 VIVFSPIFVFESVEGKFFKPLAMTLIIALLASLFVAFTFIPALSYYFLKPG-KETYSKLM 504 Query: 61 RAAKRAYAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQSLRIP 120 RA + Y +L + + + ++ V L TR+G+EF P L EG + +++ P Sbjct: 505 RAIHKVYEKVLDFTFKLRTLLYLGVFISFVFSLFLLTRIGTEFAPELEEGAVIVKAFLDP 564 Query: 121 GTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDGYIMLKPQDQWP 180 SL + + +ER FPE+ R F+ G AE + N + +I+LKPQ +W Sbjct: 565 NVSLEEGKRVATAIERE-AMKFPEVIRTFSTVGRAE-KGEAADVNYIETWILLKPQSEW- 621 Query: 181 KPRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMDVLND 240 + KTR+E +++ + LP S F+QPI++R +EL+SGVR+D+A+K+FG D +VLN+ Sbjct: 622 ETFKTREEFNEILRKRLEWLPA-SLGFTQPIKMRIDELLSGVRADIAIKIFGQDPEVLNE 680 Query: 241 TAAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTARYGLNIGDVQDTVAMAIGGKE 300 A K+ VPGA +V+ E G L + E RYG+ + +V D V +GG Sbjct: 681 LADKVKEIAESVPGAVDVQKEIQAGRLQLRIYPKWEVLERYGITVEEVLDVVKYNLGGAP 740 Query: 301 AGALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPHNDGKLSYIPLGEVADFDIAPGPN 360 G L + F I++ LPE+ R D+ + + IPL GK+ + G+VAD + PG Sbjct: 741 VGELQKDTLLFPIVMTLPEDFRGDVSKLAK--IPLFERHGKI--LTFGDVADLKVEPGLF 796 Query: 361 QVSRENGKRRVVVSANVRGRDLGSFVDDAAAGIAAQVKIPTGYWIAWGGQFENLQSATQR 420 + REN R +V NV GRD GSFV + + I +VK+PTGY+I +GGQFEN Q A ++ Sbjct: 797 VIRRENNIRFALVMLNVEGRDTGSFVKELQSRIENEVKLPTGYFITYGGQFENQQRAMKK 856 Query: 421 LQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGV 461 L I VPV++ L+FV+L+ FN+V+D L+V +PFA+ GG+ Sbjct: 857 LSIAVPVSIFLIFVMLYLNFNSVRDALVVMLNVPFAVIGGI 897