BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0040.p (366 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0825 [E] COG0421 Spermidine synthase Rso Ralstonia solanacearum 115 2e-25 NMB0869 [E] COG0421 Spermidine synthase Nme Neisseria meningitid... 110 6e-24 NMA1087 [E] COG0421 Spermidine synthase NmA Neisseria meningitid... 110 6e-24 RSc0995 [E] COG0421 Spermidine synthase Rso Ralstonia solanacearum 99 1e-20 MJ0313 [E] COG0421 Spermidine synthase Mja Methanococcus jannaschii 62 2e-09 >RSp0825 [E] COG0421 Spermidine synthase Rso Ralstonia solanacearum Length = 306 Score = 115 bits (287), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 71/195 (36%), Positives = 100/195 (51%), Gaps = 7/195 (3%) Query: 144 PFRLALGYTQTMMGFLFFHQDPVS----IAMIGLGGGSLPKYCHQHLPNSLITVLENNSD 199 P L L Y M +L F + + +G+G GS ++CH+HLP + IT +E N D Sbjct: 84 PDALDLEYIHQQMAWLLFLEPSAESDFHVVQLGMGPGSTTRFCHRHLPTARITAVEVNPD 143 Query: 200 VIAL-RDHFLIPQDDDRLSVQCVDGADFV--KRHKEKYDVLIVDGFDRVGQPAQLCSQDF 256 VIA+ R F +P DD RL V DG D+V H+ DVL VD +D V Q L + +F Sbjct: 144 VIAMARSEFALPPDDARLRVLQQDGYDWVMDPSHQGTADVLQVDCYDHVKQGPVLDTLEF 203 Query: 257 YDNAYQMLDDGGIMVVNLIDIDYVGQVYVDRIRRSFDNAVIVIPVFDSMNQVVFACKGTA 316 Y L G+M VNL D Q+ ++RI ++FD V+++P N +V A G Sbjct: 204 YQACRDTLRYPGVMTVNLFGSDESFQLNIERICKAFDYRVVILPEVTGGNTIVLAFNGPP 263 Query: 317 LQTSNKTLIRRLKEI 331 LQ S L R + + Sbjct: 264 LQASWDALRARAQAL 278 >NMB0869 [E] COG0421 Spermidine synthase Nme Neisseria meningitidis MC58 Length = 263 Score = 110 bits (275), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 66/230 (28%), Positives = 114/230 (49%), Gaps = 2/230 (0%) Query: 119 IYSNGQSAYLHFNMLSVHSEMDMQAPFRLALGYTQTMMGFLFFHQD-PVSIAMIGLGGGS 177 I G LH +V S M++ P L L Y++ MMG+L F P I IGLGGGS Sbjct: 21 ISEEGNIRSLHLGSDTVQSSMNLDHPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGS 80 Query: 178 LPKYCHQHLPNSLITVLENNSDVIAL-RDHFLIPQDDDRLSVQCVDGADFVKRHKEKYDV 236 ++ +LP++ T ++ N VIA+ R+ F +P + ++ + DGA+++K + DV Sbjct: 81 FARWIDTYLPDTRQTAVDINPQVIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDV 140 Query: 237 LIVDGFDRVGQPAQLCSQDFYDNAYQMLDDGGIMVVNLIDIDYVGQVYVDRIRRSFDNAV 296 ++VDGFD L + F+ + L GI V N D Q +++R+ F+ V Sbjct: 141 ILVDGFDGEQIIDALVEEPFFRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRV 200 Query: 297 IVIPVFDSMNQVVFACKGTALQTSNKTLIRRLKEIVGGHTVDLCQVLQSI 346 + +P N V A + + + + L +R ++ + +D ++L + Sbjct: 201 LELPAESHGNVAVMAFQSSPKEQNIDKLKKRADKLSNAYGLDFHRMLAGL 250 >NMA1087 [E] COG0421 Spermidine synthase NmA Neisseria meningitidis Z2491 Length = 263 Score = 110 bits (275), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 66/230 (28%), Positives = 114/230 (49%), Gaps = 2/230 (0%) Query: 119 IYSNGQSAYLHFNMLSVHSEMDMQAPFRLALGYTQTMMGFLFFHQD-PVSIAMIGLGGGS 177 I G LH +V S M++ P L L Y++ MMG+L F P I IGLGGGS Sbjct: 21 ISEEGNIRSLHLGSDTVQSSMNLDHPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGS 80 Query: 178 LPKYCHQHLPNSLITVLENNSDVIAL-RDHFLIPQDDDRLSVQCVDGADFVKRHKEKYDV 236 ++ +LP++ T ++ N VIA+ R+ F +P + ++ + DGA+++K + DV Sbjct: 81 FARWIDTYLPDTRQTAVDINPQVIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDV 140 Query: 237 LIVDGFDRVGQPAQLCSQDFYDNAYQMLDDGGIMVVNLIDIDYVGQVYVDRIRRSFDNAV 296 ++VDGFD L + F+ + L GI V N D Q +++R+ F+ V Sbjct: 141 ILVDGFDGEQIIDALVEEPFFRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRV 200 Query: 297 IVIPVFDSMNQVVFACKGTALQTSNKTLIRRLKEIVGGHTVDLCQVLQSI 346 + +P N V A + + + + L +R ++ + +D ++L + Sbjct: 201 LELPAESHGNVAVMAFQSSPKEQNIDKLKKRADKLSNAYGLDFHRMLAGL 250 >RSc0995 [E] COG0421 Spermidine synthase Rso Ralstonia solanacearum Length = 295 Score = 99.4 bits (246), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 66/202 (32%), Positives = 94/202 (46%), Gaps = 9/202 (4%) Query: 127 YLHFNMLSVHSEMDMQAPFRLALGYTQTMMGFLFFHQDPVS-----IAMIGLGGGSLPKY 181 YLHF V M + P + L Y Q MM +L F DP + + +GLG +L K+ Sbjct: 56 YLHFGTEWVQGAMRLSKPDAIELEYAQQMMAWLLF-LDPAARPDFHVVQLGLGAAALTKF 114 Query: 182 CHQHLPNSLITVLE-NNSDVIALRDHFLIPQDDDRLSVQCVDGADFV--KRHKEKYDVLI 238 CH+ L + +T +E N + +IA F +P DD RL V D D+V H D L Sbjct: 115 CHRQLAPARVTAVELNPAVIIAAHSMFGLPFDDSRLGVIEQDAYDWVTDANHHGTVDALQ 174 Query: 239 VDGFDRVGQPAQLCSQDFYDNAYQMLDDGGIMVVNLIDIDYVGQVYVDRIRRSFDNAVIV 298 +D +D + L + FY Q+L G+M +NL ++RI +FDN V+V Sbjct: 175 IDLYDATARGPVLDTPAFYRACRQVLRAPGVMTINLFGDHTSFPRNIERICDAFDNRVLV 234 Query: 299 IPVFDSMNQVVFACKGTALQTS 320 P N + A G LQ Sbjct: 235 FPEVHDGNVIALAFSGPPLQVG 256 >MJ0313 [E] COG0421 Spermidine synthase Mja Methanococcus jannaschii Length = 293 Score = 62.4 bits (150), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 37/118 (31%), Positives = 64/118 (54%), Gaps = 6/118 (5%) Query: 159 LFFHQDPVSIAMIGLGGGSLPKYCHQHLPNSLITVLENNSDVIALRDHFLIPQ-----DD 213 LF H +P ++ +IG G G + +H + +E + VI ++ P+ D+ Sbjct: 78 LFTHPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVIEACKKYM-PKLSCEIDN 136 Query: 214 DRLSVQCVDGADFVKRHKEKYDVLIVDGFDRVGQPAQLCSQDFYDNAYQMLDDGGIMV 271 +++++ DG +V ++KYDV+IVD D VG L ++FY N ++ L+D GIMV Sbjct: 137 EKVNLIITDGIKYVAETEKKYDVIIVDCPDPVGPAKGLFEKEFYKNVFKCLNDDGIMV 194