BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0004.p (509 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0421 [GEPR] COG0477 Permeases of the major facilitator superf... 387 e-107 NMA1673 [GEPR] COG0477 Permeases of the major facilitator superf... 346 9e-95 NMB1461 [GEPR] COG0477 Permeases of the major facilitator superf... 321 2e-87 yajR [GEPR] COG0477 Permeases of the major facilitator superfami... 308 2e-83 STM0436 [GEPR] COG0477 Permeases of the major facilitator superf... 306 6e-83 >RSc0421 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 387 Score = 387 bits (993), Expect = e-107, Method: Compositional matrix adjust. Identities = 216/385 (56%), Positives = 277/385 (71%), Gaps = 6/385 (1%) Query: 22 MSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIYGLAQSFG 81 M+G E+RA+ SLA IFALRMLGLF+I+PVFAV AK + G + +V A+G+YGL Q+ Sbjct: 1 MTGFELRAATSLAGIFALRMLGLFMIMPVFAVFAKSLPDGGNTQLVAFAIGVYGLTQAVL 60 Query: 82 QIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFI 141 IP+G SD++GRK VIV GL++FA GS VAA + + + GRAIQGAGAIS+AV AF+ Sbjct: 61 YIPYGWLSDRFGRKPVIVAGLLIFAAGSLVAAFSHSVAGIAVGRAIQGAGAISSAVIAFV 120 Query: 142 ADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLF 201 AD TREEHRTKAMAM+GGSIG++FA+++V++P+++ IGM G+F G+LAL AIGVVL+ Sbjct: 121 ADLTREEHRTKAMAMIGGSIGVSFAVAIVSAPVIFRWIGMPGMFLAIGILALIAIGVVLW 180 Query: 202 VVPDAPMVKAR-RVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYAGLPVAE 260 VVPDAP A P EVL + EL+RLNFGVFALH TQ A+FVVLP L + AGLPV Sbjct: 181 VVPDAPRPAAHVSAPFREVLHNRELLRLNFGVFALHATQTALFVVLPHML-ETAGLPVDS 239 Query: 261 HWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHFAAHWWV 320 HWKIYLPV+ SF+ M+P + EKRG MK V ++A++L+++ Q+ + A+ Sbjct: 240 HWKIYLPVMGVSFVLMVPAIIAAEKRGKMKAVLLSAVALVMVAQLALGEAQPTLAS---- 295 Query: 321 LVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALGGWLKQF 380 L L +F+ FN+LEA QPSLVS+ AP KG A+GVYNT QALGLF GGA GGW+ Sbjct: 296 LTVALLVYFLGFNVLEASQPSLVSKYAPGVRKGAAMGVYNTTQALGLFTGGAGGGWILLH 355 Query: 381 AGAPSVFVMCAVATLTWLIIASNMK 405 AG +VF CA WLIIAS M+ Sbjct: 356 AGQHAVFFTCAGLAAAWLIIASPMR 380 >NMA1673 [GEPR] COG0477 Permeases of the major facilitator superfamily NmA Neisseria meningitidis Z2491 Length = 461 Score = 346 bits (887), Expect = 9e-95, Method: Compositional matrix adjust. Identities = 191/383 (49%), Positives = 269/383 (70%), Gaps = 12/383 (3%) Query: 26 EVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGE-SAAMVGLAMGIYGLAQSFGQIP 84 E RAS +L+ ++ALRMLG+FL+LPV AV+A + G E + +VGLAMGIYGL Q+ Q+P Sbjct: 14 EWRASTTLSGVYALRMLGMFLVLPVLAVYAASLPGAEGNKTLVGLAMGIYGLTQALLQLP 73 Query: 85 FGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFIADS 144 G+ASDK+GRK+ I VGL++FA GSF+AA A + ++A RAIQGAGA+SAAVTA +AD Sbjct: 74 LGIASDKFGRKKTIYVGLIVFAAGSFLAAAADTLPMLVAARAIQGAGAVSAAVTALLADL 133 Query: 145 TREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLFVVP 204 TR+ RT+AMAM+G SIGLTF++SLV +P++ +A+G+ G+F LTG+L +A+IGVV ++ P Sbjct: 134 TRDGVRTRAMAMIGLSIGLTFSVSLVVAPVIADAVGVRGLFLLTGILTVASIGVVAWMTP 193 Query: 205 DAPMVK------ARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYAGLPV 258 D + K A+ +GEVL++ L+ L+FG+FALH QMA+F LP A+ Q GL Sbjct: 194 DPEVSKLHEDTQAQPSRIGEVLKNRRLLTLDFGIFALHAAQMALFTALPFAMTQL-GLEK 252 Query: 259 AEHWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHFAAHW 318 +HWK+YLP + + M+P + +GE R +KQVF+ I+ + Q+G+ + + Sbjct: 253 IQHWKVYLPSTITGLVVMVPLIIVGETRNKLKQVFILGIACIAAAQLGLLSGMRSVG--- 309 Query: 319 WVLVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALGGWLK 378 ++ L +FI FN+LEA PS+VS+IAP+ KGTA+GVYNT+Q+LGLF GG GG L Sbjct: 310 -LITAYLVVYFIGFNVLEASLPSMVSKIAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLF 368 Query: 379 QFAGAPSVFVMCAVATLTWLIIA 401 Q G VF C++ L WL+IA Sbjct: 369 QKYGFSGVFAFCSILMLLWLVIA 391 >NMB1461 [GEPR] COG0477 Permeases of the major facilitator superfamily Nme Neisseria meningitidis MC58 Length = 461 Score = 321 bits (823), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 192/383 (50%), Positives = 267/383 (69%), Gaps = 12/383 (3%) Query: 26 EVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGE-SAAMVGLAMGIYGLAQSFGQIP 84 E RAS +L+ ++ALRMLG+FL+LPV AV+A + G E + +VGLAMGIYGL Q+ Q+P Sbjct: 14 EWRASTTLSGVYALRMLGMFLVLPVLAVYAASLPGAEGNKTLVGLAMGIYGLTQALLQLP 73 Query: 85 FGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFIADS 144 G+ASDK+GRK+ I GLV+FA GSF+AA A + ++A RAIQGAGA+SAAVTA +AD Sbjct: 74 LGIASDKFGRKKTIYAGLVVFAAGSFLAAAADTLPMLVAARAIQGAGAVSAAVTALLADL 133 Query: 145 TREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLFVVP 204 TR+ RT+AMAM+G SIGLTF++SLV +P++ +A+G+ G+F LTG+L + +IGVV ++ P Sbjct: 134 TRDGVRTRAMAMIGLSIGLTFSVSLVVAPVIADAVGVRGLFMLTGILTVISIGVVAWMTP 193 Query: 205 DAPMVK------ARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQYAGLPV 258 D + K A+ +GEVL++ L+ L+FG+FALH QMA+F LP A+ Q GL Sbjct: 194 DPEVSKLHEDTQAQPSRIGEVLKNRRLLTLDFGIFALHAAQMALFTALPFAMTQL-GLEK 252 Query: 259 AEHWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLAHFAAHW 318 +HWK+YLP + + M+P + +GE R +KQVFV I + Q+G+ + + Sbjct: 253 IQHWKVYLPSTITGLVVMVPLIIVGETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVG--- 309 Query: 319 WVLVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGALGGWLK 378 ++ L +FI FN+LEA PS+VS+IAP+ KGTA+GVYNT+Q+LGLF GGA GG L Sbjct: 310 -LITAYLVVYFIGFNVLEASLPSMVSKIAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLF 368 Query: 379 QFAGAPSVFVMCAVATLTWLIIA 401 Q G VF C++ L WL+IA Sbjct: 369 QKYGFSGVFAFCSILMLLWLVIA 391 >yajR [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 456 Score = 308 bits (790), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 181/451 (40%), Positives = 268/451 (59%), Gaps = 32/451 (7%) Query: 22 MSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIYGLAQSFG 81 M+ E RA+ L ++F+LRMLG+F++LPV + + G S A++G+A+GIYGL Q+ Sbjct: 8 MTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQGA-SEALIGIAIGIYGLTQAVF 66 Query: 82 QIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFI 141 QIPFG+ SD+ GRK +IV GL +FA GS +AA + I +I GRA+QG+GAI+AAV A + Sbjct: 67 QIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIWGIILGRALQGSGAIAAAVMALL 126 Query: 142 ADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLF 201 +D TRE++RTKAMA +G S G+TFA+++V P++ +G+ +F + +LA I + ++ Sbjct: 127 SDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITHKLGLHALFWMIAILATTGIALTIW 186 Query: 202 VVPDAP---------MVKARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQ 252 VVP++ MVK +VL L++LNFG+ LH+ M+ FV LP L Sbjct: 187 VVPNSSTHVLNRESGMVKGS---FSKVLAEPRLLKLNFGIMCLHILLMSTFVALPGQLAD 243 Query: 253 YAGLPVAEHWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLA 312 AG P AEHWK+YL +L +F ++P + E + MKQVFV + L+++ ++ +W Sbjct: 244 -AGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRKMKQVFVFCVGLIVVAEIVLWNAQT 302 Query: 313 HFAAHWWVLVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGA 372 F W LV + FF+AFN++EA PSL+S+ +PA KGTA+GVY+T Q LG+ GG+ Sbjct: 303 QF----WQLVVGVQLFFVAFNLMEALLPSLISKESPAGYKGTAMGVYSTSQFLGVAIGGS 358 Query: 373 LGGWLKQFAGAPSVFVMCAVATLTWLIIASNMK----------NLPRRGAQXRGRPARKR 422 LGGW+ VF+ A+ WL +AS MK +P A R Sbjct: 359 LGGWINGMFDGQGVFLAGAMLAAVWLTVASTMKEPPYVSSLRIEIPANIAANEALKVRLL 418 Query: 423 NGLGIKSVIKFSEIQEKQWHRSIKSSSSATS 453 GIK V+ I E++ +K S T+ Sbjct: 419 ETEGIKEVL----IAEEEHSAYVKIDSKVTN 445 >STM0436 [GEPR] COG0477 Permeases of the major facilitator superfamily Sty Salmonella typhimurium LT2 Length = 449 Score = 306 bits (785), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 170/396 (42%), Positives = 255/396 (64%), Gaps = 18/396 (4%) Query: 22 MSGAEVRASASLASIFALRMLGLFLILPVFAVHAKGMSGGESAAMVGLAMGIYGLAQSFG 81 M+ E+RA+ L ++F+LRMLG+F++LPV + + G S A++G+A+GIYGLAQ+ Sbjct: 1 MTPGELRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQGA-SEALIGIAIGIYGLAQAIF 59 Query: 82 QIPFGMASDKYGRKRVIVVGLVLFALGSFVAAGAQDIGWVIAGRAIQGAGAISAAVTAFI 141 QIPFG+ SD+ GRK +IV GL +F GS +AA + I +I GRA+QG+GAI+AAV A + Sbjct: 60 QIPFGLLSDRIGRKPLIVGGLAVFVAGSVIAALSHSIWGIILGRALQGSGAIAAAVMALL 119 Query: 142 ADSTREEHRTKAMAMVGGSIGLTFALSLVASPLLYEAIGMGGIFGLTGVLALAAIGVVLF 201 +D TRE++RTKAMA +G S G+TFA+++V P++ ++G+ +F + LA I + ++ Sbjct: 120 SDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIVTHSLGLNALFWMIAALATLGILLTIW 179 Query: 202 VVPDAP---------MVKARRVPLGEVLRHGELMRLNFGVFALHMTQMAMFVVLPAALVQ 252 VVP++ MVK +VL L++LNFG+ LH+ M+ FV LP L Sbjct: 180 VVPNSTNHVLNRESGMVKGS---FSKVLAEPRLLKLNFGIMCLHILLMSTFVALPGQLAD 236 Query: 253 YAGLPVAEHWKIYLPVVLASFIFMLPPVFIGEKRGLMKQVFVAAISLLLLVQVGMWATLA 312 AG P AEHWK+YL ++ +F ++P + E + MKQVF+ + L+++ ++ +W Sbjct: 237 -AGFPAAEHWKVYLATMVIAFAAVVPFIIYAEVKRRMKQVFLFCVGLIVVAEIVLWGAGQ 295 Query: 313 HFAAHWWVLVFLLFAFFIAFNILEACQPSLVSRIAPAAAKGTALGVYNTLQALGLFCGGA 372 HF W LV + FF+AFN++EA PSL+S+ +PA KGTA+GVY+T Q LG+ GG+ Sbjct: 296 HF----WELVIGVQLFFLAFNLMEALLPSLISKESPAGYKGTAMGVYSTSQFLGVALGGS 351 Query: 373 LGGWLKQFAGAPSVFVMCAVATLTWLIIASNMKNLP 408 LGGW+ +VF+ AV + WL +AS MK P Sbjct: 352 LGGWIDGTFDGQTVFLAGAVLAMVWLAVASTMKEPP 387