BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0204.p (398 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2450 [I] COG1398 Fatty-acid desaturase Rso Ralstonia solanace... 584 e-166 PA0286 [I] COG1398 Fatty-acid desaturase Pae Pseudomonas aeruginosa 416 e-116 all4991 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 87 1e-16 all1599 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 86 1e-16 sll0541 [I] COG1398 Fatty-acid desaturase Syn Synechocystis 85 3e-16 >RSc2450 [I] COG1398 Fatty-acid desaturase Rso Ralstonia solanacearum Length = 398 Score = 584 bits (1506), Expect = e-166, Method: Compositional matrix adjust. Identities = 269/396 (67%), Positives = 331/396 (83%), Gaps = 1/396 (0%) Query: 3 VDSILDFLSNGLLHATGWQIFFYTLIVTHITIAGVTIYLHRCQAHRALDLHAIPSHFFRL 62 +DS L FL+NG L+ WQI +TL+VTH+TIA VTI+LHRCQAHRALDLH +H FR Sbjct: 4 IDSFLSFLANGTLNWAWWQILIFTLVVTHMTIASVTIFLHRCQAHRALDLHPAAAHVFRF 63 Query: 63 WLWMTTGMVTKEWAAIHRKHHAKCETEEDPHSPQVRGIKKVLLEGAELYRTESKNAETME 122 WLW+TTGMVTKEWAAIHRKHHAKCETEEDPHSPQ RGI+KVLLEGAELYR E+KN ET++ Sbjct: 64 WLWLTTGMVTKEWAAIHRKHHAKCETEEDPHSPQTRGIRKVLLEGAELYRAEAKNRETIQ 123 Query: 123 KYGHGTPDDWVERNVYSRFGWQGVGLMLIINVMLFGVIGLTVWAVQMLWIPITAAGIING 182 K+GHGTP+DW+E+N+YS++ WQGVGLMLI++V++FG +GLTVWAVQMLWIPI AAGIING Sbjct: 124 KFGHGTPNDWIEQNLYSKYTWQGVGLMLILDVLMFGTLGLTVWAVQMLWIPIHAAGIING 183 Query: 183 IGHFWGYRNYDCNDAATNVFPWGIIIGGEELHNNHHTFGTSAKLSSKWYEFDIGWMYIRI 242 +GH+WGYRN+DC DA+TNVFPWG+IIGGEELHNNHHT+ TSAK S KWYEFD+GW YIR Sbjct: 184 LGHYWGYRNFDCEDASTNVFPWGLIIGGEELHNNHHTYATSAKFSIKWYEFDVGWGYIRA 243 Query: 243 LEIMGLAKVKKIAPAPKFDRQKLVADFDTLQSVIANRYDVMSKYAKSLKRAWADELEHLA 302 LE++GLAKVKK+AP +F K V D +TL+++IANRYDVM++YAK++K A+ EL L Sbjct: 244 LEMVGLAKVKKVAPKARFGDVKPV-DQNTLEAIIANRYDVMARYAKTVKVAYRQELAKLK 302 Query: 303 EKAKLESRFLKSSKKLLQREPAKLEDGQKQQLSELFTHSKALQTMHEMRVELGAIWGRSN 362 + +E R LK ++K R+ AKL Q++QLS++ + AL T EMR +LGA+WGRSN Sbjct: 303 DARDVEYRALKPARKWFHRDEAKLGAPQREQLSQIVSDHSALHTFVEMRRDLGALWGRSN 362 Query: 363 STREQLLQQLQDWCVRAEASGIHALRDFALRLRSYA 398 +TREQLL QLQ+WC RAE SGIHAL++FA+RLR YA Sbjct: 363 ATREQLLHQLQEWCKRAEESGIHALQEFAVRLRRYA 398 >PA0286 [I] COG1398 Fatty-acid desaturase Pae Pseudomonas aeruginosa Length = 392 Score = 416 bits (1070), Expect = e-116, Method: Compositional matrix adjust. Identities = 197/388 (50%), Positives = 271/388 (69%), Gaps = 4/388 (1%) Query: 12 NGLLHATGWQIFFYTLIVTHITIAGVTIYLHRCQAHRALDLHAIPSHFFRLWLWMTTGMV 71 NG L + WQ+ TL++THITI VT+YLHR AHRALDLH HFFR WLW+TTGM Sbjct: 4 NGFLDLSPWQLVAVTLVLTHITIVSVTVYLHRYSAHRALDLHPALQHFFRFWLWLTTGMN 63 Query: 72 TKEWAAIHRKHHAKCETEEDPHSPQVRGIKKVLLEGAELYRTESKNAETMEKYGHGTPDD 131 T+EW AIHRKHHAKCET EDPHSP +G+ VL GAELY+ E+KN +T+ YG PDD Sbjct: 64 TREWTAIHRKHHAKCETVEDPHSPVHKGLFTVLRAGAELYKAEAKNQDTLRIYGKNCPDD 123 Query: 132 WVERNVYSRFGWQGVGLMLIINVMLFGVIGLTVWAVQMLWIPITAAGIINGIGHFWGYRN 191 W+ERN+YSRF GV LM +I++ LFG +GLTVWAVQM+WIP AAG++NG+GH GYRN Sbjct: 124 WIERNLYSRFPIGGVVLMALIDLALFGALGLTVWAVQMVWIPFWAAGVVNGLGHAVGYRN 183 Query: 192 YDCNDAATNVFPWGIIIGGEELHNNHHTFGTSAKLSSKWYEFDIGWMYIRILEIMGLAKV 251 ++C DAATN+ PWGI+IGGEELHNNHHT+ SAKLS K +EFD+GW +I++ + LA+V Sbjct: 184 FECRDAATNLVPWGILIGGEELHNNHHTYPNSAKLSVKKWEFDLGWAWIKLFSFLRLARV 243 Query: 252 KKIAPAPKFDRQKLVADFDTLQSVIANRYDVMSKYAKSLKRAWADELEHLAEKAKLESRF 311 ++AP K D DT +++ NR+ +M++Y K + A ++ KA R Sbjct: 244 ARVAPIAHRVEGKHSLDMDTAMAILNNRFQIMAQYRKLVI---APLVKQEVAKADESVRH 300 Query: 312 L-KSSKKLLQREPAKLEDGQKQQLSELFTHSKALQTMHEMRVELGAIWGRSNSTREQLLQ 370 L + +K+LL RE + L+D ++ + HS+AL+ ++E R+ L IW ++++ +L Sbjct: 301 LFRRAKRLLSRETSLLQDRHHVRIDSMLAHSQALKVIYEKRLALQQIWAKTSANGHDMLA 360 Query: 371 QLQDWCVRAEASGIHALRDFALRLRSYA 398 ++DW AEASGI +L++FA +L++Y+ Sbjct: 361 AIKDWVHEAEASGIQSLKEFAAQLKTYS 388 >all4991 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 Length = 285 Score = 86.7 bits (213), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 65/197 (32%), Positives = 98/197 (49%), Gaps = 19/197 (9%) Query: 75 WAAIHRKHHAKCE-TEEDPHSPQVRGIKKVLLEGAELYRTESKNAETMEKYGHGTPD--- 130 W HR+HHA E DP+S Q RG + R+E + E +KY PD Sbjct: 98 WIGGHRQHHAHTEDVYLDPYSSQ-RGFWWSHMLWILYPRSEFFDYEIYQKYA---PDLAR 153 Query: 131 ----DWVERNVYSRFGWQGVGLMLI-INVMLFGVIGLTVWAVQMLWIPITAAGIINGIGH 185 W++R Y GLML I F + G+ + AV +LW + +N H Sbjct: 154 QPFYRWLDR--YFLLLQIPFGLMLYAIGGWSFVIYGVVLRAV-LLW---HSTWFVNSATH 207 Query: 186 FWGYRNYDCNDAATNVFPWGIIIGGEELHNNHHTFGTSAKLSSKWYEFDIGWMYIRILEI 245 WGYR ++ +D A N++ I+ GE HNNHHT+ AK +W+E D+ W I++L+ Sbjct: 208 MWGYRTFNADDNARNLWWVSIVTYGEGWHNNHHTYPNVAKAGFQWWEVDVTWWSIKLLQT 267 Query: 246 MGLAKVKKIAPAPKFDR 262 +GLAK + P+ + ++ Sbjct: 268 LGLAKKVVLPPSQRMNQ 284 >all1599 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 Length = 272 Score = 86.3 bits (212), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 64/226 (28%), Positives = 107/226 (47%), Gaps = 17/226 (7%) Query: 36 GVTIYLHRCQAHRALDLHAIPSHFFRLWLWMTTGMVTKEWAAIHRKHHAKCETEEDPHSP 95 G+T+ HR HR+ +F L + EW HR HH +T+ DPH Sbjct: 54 GITLGFHRLVTHRSFQTPKWLEYFLVLCGTLACQGGPIEWVGTHRIHHLHSDTDPDPHDS 113 Query: 96 QVRGIKKVLLEGAELYRTESKNAETMEKYGHGTPDDWVERNVYSRFGWQGVGLMLII--- 152 +G + G +Y + S + ++ +D V + + F + + L L++ Sbjct: 114 N-KGFWWSHI-GWLIYHSPSH--ADVPRFTKDIAEDPVYQFLQKYFIFIQIALGLLLLYL 169 Query: 153 ---NVMLFGVIGLTVWAVQMLWIPITAAGIINGIGHFWGYRNYDCNDAATNVFPWGIIIG 209 + +++GV VW W+ +N H +GYR YD D +TN + +++ Sbjct: 170 GGWSFVVWGVFFRIVWVYHCTWL-------VNSATHKFGYRTYDAGDRSTNCWWVAVLVF 222 Query: 210 GEELHNNHHTFGTSAKLSSKWYEFDIGWMYIRILEIMGLAKVKKIA 255 GE HNNHH F SA+ +W+E D+ WM +++L+I+GLA K+A Sbjct: 223 GEGWHNNHHAFQYSARHGLEWWEVDLTWMTVQLLQILGLATNVKLA 268 >sll0541 [I] COG1398 Fatty-acid desaturase Syn Synechocystis Length = 318 Score = 85.1 bits (209), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 60/227 (26%), Positives = 104/227 (45%), Gaps = 17/227 (7%) Query: 36 GVTIYLHRCQAHRALDLHAIPSHFFRLWLWMTTGMVTKEWAAIHRKHHAKCETEEDPHSP 95 G+T+ HRC +HR+ ++ + F + + EW +HR HH +T DPH Sbjct: 95 GITLGFHRCISHRSFNVPKWLEYIFVICGTLACQGGVFEWVGLHRMHHKFSDTTPDPHDS 154 Query: 96 QVRGIKKVLLEGAELYRTESKNAETMEKYGHGTPDDWVERNVYSRFGWQGVGLMLIINVM 155 +G + G ++ +K + +Y DD + + V L LI+ + Sbjct: 155 N-KGFWWSHI-GWMMFEIPAK--ADIPRYTKDIQDDKFYQFCQNNLILIQVALGLILFAL 210 Query: 156 ------LFGVIGLTVWAVQMLWIPITAAGIINGIGHFWGYRNYDCNDAATNVFPWGIIIG 209 ++G+ V+ W +N H +GY +++ ND + N + ++ Sbjct: 211 GGWPFVIWGIFVRLVFVFHFTWF-------VNSATHKFGYVSHESNDYSRNCWWVALLTF 263 Query: 210 GEELHNNHHTFGTSAKLSSKWYEFDIGWMYIRILEIMGLAKVKKIAP 256 GE HNNHH + SA+ +W+E D+ WM I+ L ++GLAK K+ P Sbjct: 264 GEGWHNNHHAYQYSARHGLQWWEVDLTWMTIKFLSLLGLAKDIKLPP 310