BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0119.p (464 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cj0339 [GEPR] COG0477 Permeases of the major facilitator superfa... 311 3e-84 STM0212 [GEPR] COG0477 Permeases of the major facilitator superf... 292 1e-78 ECs2778 [GEPR] COG0477 Permeases of the major facilitator superf... 292 1e-78 shiA [GEPR] COG0477 Permeases of the major facilitator superfami... 291 3e-78 ZshiA [GEPR] COG0477 Permeases of the major facilitator superfam... 291 3e-78 >Cj0339 [GEPR] COG0477 Permeases of the major facilitator superfamily Cje Campylobacter jejuni Length = 453 Score = 311 bits (796), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 170/420 (40%), Positives = 259/420 (61%), Gaps = 10/420 (2%) Query: 46 ALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPMIGTLAAFGSYALGFVVRPLGGF 105 A+ S +GTA+E+ DF LYG AAA +F+ +FF + P+I L +F +Y +GF+ RP+G Sbjct: 26 AVFSCWLGTAMEYADFALYGLAAATIFSEVFFPEQTPVIALLLSFVTYGIGFIARPIGAL 85 Query: 106 VFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASIGIAAPILLTLLRLIQGFAAGAE 165 FGH+GD+ GRKNV++ T+ +MG+STTLIGF+P+YA IG+ API L LR +QGF AGAE Sbjct: 86 FFGHLGDKHGRKNVMMATIALMGISTTLIGFIPSYAVIGVWAPICLVALRFMQGFGAGAE 145 Query: 166 FGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGVFTLVRQMPEADFMNYGWRIPFI 225 G M AEYAP +RG +S +G G +L++ V+ L+ QM EA F +GWR+PF+ Sbjct: 146 LSGGTVMLAEYAPSKRRGLVSSVIALGSNSGTLLASFVWLLMVQMDEASFKEWGWRVPFM 205 Query: 226 LSSIGLAVGMYIRLYVLESPAFEKIKSK--------KAVQKSPVSAVWKHHRRHLFIAIG 277 S + +YIR +V E+P FEK K++ + K + W+ R + +G Sbjct: 206 GSILIALFAVYIRFHVKETPVFEKQKNEMMKLRLNNEKHMKKDERSFWQRS-RAFWTMVG 264 Query: 278 ARFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVLIGAGLGALLNPFWGMLSDRIGRKP 337 R EN Y+ Q + + Y+T L +D S+ AV+I + +G L+ P G LSD+ GR Sbjct: 265 LRIGENGPSYLAQGFIVGYVTKILLLDKSVATTAVMIASLVGFLVIPLAGYLSDKFGRCI 324 Query: 338 VYILGILVMILGSWPFFMMLETRETPMIIAGFVMMIVFGLYPMFSVQAAFFAELFPPSVR 397 Y + L++++ ++P FM+L+++ ++I ++ + +F VQAA+ ELF R Sbjct: 325 TYRMFCLLLMIYAFPAFMLLDSKNEIIVILTIIVGMSLASLGIFGVQAAWGVELFGVKNR 384 Query: 398 MSGICMARELSSVITGGMAPVIATGLLAYYGGSYYPIIGYLVFMGGVALIALLMAPETRG 457 + + A+EL S+++GG AP++A+ LLAYY GS++PI Y V G+ + APETRG Sbjct: 385 YTKMAFAKELGSILSGGTAPMVASALLAYY-GSWWPIATYFVLTAGIGFVTTFFAPETRG 443 >STM0212 [GEPR] COG0477 Permeases of the major facilitator superfamily Sty Salmonella typhimurium LT2 Length = 433 Score = 292 bits (748), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 168/420 (40%), Positives = 254/420 (60%), Gaps = 8/420 (1%) Query: 42 QRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPMIGTLAAFGSYALGFVVRP 101 +RV+ S+ +G ++E+YDF LYG A+A+VF LFF P+ TL++F S+ +GF+ RP Sbjct: 10 RRVVV--SAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFISRP 67 Query: 102 LGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASIGIAAPILLTLLRLIQGFA 161 LG FG+ GD +GRKN L+ITL MG T LIG LP+YASIG AP LL +LR +QGF Sbjct: 68 LGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILRFLQGFM 127 Query: 162 AGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGVFTLVRQMPEADFMNYGWR 221 G E+GGA+ M EYA RG ++ G G +L+ GVF +V Q+P+ +++GWR Sbjct: 128 VGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLTGQLLATGVFIVVTQLPQEALLSWGWR 187 Query: 222 IPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKAV------QKSPVSAVWKHHRRHLFIA 275 IPF+LS++ + G+Y+R + E+P F K ++A+ ++ PV V + R + + Sbjct: 188 IPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQWRSILLI 247 Query: 276 IGARFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVLIGAGLGALLNPFWGMLSDRIGR 335 I RF+E+ ++ V+A+S+ T QL I + L V+ L ++ +G++SDR G Sbjct: 248 IILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGIMSDRRGC 307 Query: 336 KPVYILGILVMILGSWPFFMMLETRETPMIIAGFVMMIVFGLYPMFSVQAAFFAELFPPS 395 + VYI G L + ++PFF +LE+R ++ G+V++I G + +VQ +FFA LF P Sbjct: 308 RQVYIFGALFVAAMAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFFAGLFHPP 367 Query: 396 VRMSGICMARELSSVITGGMAPVIATGLLAYYGGSYYPIIGYLVFMGGVALIALLMAPET 455 VR SG + +L +V+ GG P IA L A Y S+ + GY+V + A +APET Sbjct: 368 VRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDNSWALVAGYVVLTALASAFAAKIAPET 427 >ECs2778 [GEPR] COG0477 Permeases of the major facilitator superfamily Ecs Escherichia coli O157:H7 Length = 438 Score = 292 bits (747), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 175/446 (39%), Positives = 254/446 (56%), Gaps = 20/446 (4%) Query: 17 VSEHPTERDASNAADRANAGVGLERQRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLF 76 +S P ER S L R R AL S G ++WYDF LYG AA+VFN F Sbjct: 6 ISTRPDERTVS-----------LSRARRAAL-GSFAGAVVDWYDFLLYGITAALVFNREF 53 Query: 77 FTKLDPMIGTLAAFGSYALGFVVRPLGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGF 136 F ++ P +GTLAAF ++ +GF+ RPLGG +FGH GDR+GRK +L++T+ MMG++T LIG Sbjct: 54 FPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGI 113 Query: 137 LPTYASIGIAAPILLTLLRLIQGFAAGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALG 196 LP++++IG APILL LR IQGFA G E+GGA +S E AP NK+ +Y+S VG +G Sbjct: 114 LPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVG 173 Query: 197 VVLSAGVFTLVRQM-PEADFMNYGWRIPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKA 255 ++LS G+ +L+ M + F+++GWRIPF+ S + + +++R + ES FE+ + +A Sbjct: 174 LLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQA 233 Query: 256 VQKS--PVSAVWKHHRRHLFIAIGARFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVL 313 K PV H I R E YI +AL+Y T + + + L L Sbjct: 234 AAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGL 293 Query: 314 IGAGLGALLNPFWGMLSDRIGRKPVYILGILVMILGSWPFFMMLETRETPMIIAGFVMMI 373 + GL L P + L+DR GR+ VYI G L+ L ++PFFM LE + I+ +M+ Sbjct: 294 LVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLA 353 Query: 374 VFGLYPMFSVQAAFFAELFPPSVRMSGICMARELSSVITGGMAPVIATGLLAYYGGSYYP 433 + VQ F E+F S R SG + +++SV+ GG P IA L+ Y+ G+++ Sbjct: 354 NIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHS 413 Query: 434 IIGYLVFMGGV---ALIALLMAPETR 456 + YL + G A+ ALLM R Sbjct: 414 VAIYL--LAGCLISAMTALLMKDNQR 437 >shiA [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 438 Score = 291 bits (744), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 173/439 (39%), Positives = 256/439 (58%), Gaps = 10/439 (2%) Query: 25 DASNAADRANAG-VGLERQRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPM 83 D++ + R + G + L R R AL S G ++WYDF LYG AA+VFN FF ++ P Sbjct: 2 DSTLISTRPDEGTLSLSRARRAAL-GSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPA 60 Query: 84 IGTLAAFGSYALGFVVRPLGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASI 143 +GTLAAF ++ +GF+ RPLGG +FGH GDR+GRK +L++T+ MMG++T LIG LP++++I Sbjct: 61 MGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTI 120 Query: 144 GIAAPILLTLLRLIQGFAAGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGV 203 G APILL LR IQGFA G E+GGA +S E AP NK+ +Y+S VG +G++LS G+ Sbjct: 121 GWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVGLLLSTGL 180 Query: 204 FTLVRQM-PEADFMNYGWRIPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKAVQKS--P 260 +L+ M + F+++GWRIPF+ S + + +++R + ES FE+ + +A K P Sbjct: 181 VSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQHYQAAAKKRIP 240 Query: 261 VSAVWKHHRRHLFIAIGARFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVLIGAGLGA 320 V H I R E YI +AL+Y T + + + L L+ GL Sbjct: 241 VIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSC 300 Query: 321 LLNPFWGMLSDRIGRKPVYILGILVMILGSWPFFMMLETRETPMIIAGFVMMIVFGLYPM 380 L P + L+DR GR+ VYI G L+ L ++PFFM LE + I+ +M+ + Sbjct: 301 LTIPCFAWLADRFGRRRVYITGTLIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMV 360 Query: 381 FSVQAAFFAELFPPSVRMSGICMARELSSVITGGMAPVIATGLLAYYGGSYYPIIGYLVF 440 VQ F E+F S R SG + +++SV+ GG P IA L+ Y+ G+++ + YL Sbjct: 361 VCVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYL-- 418 Query: 441 MGGV---ALIALLMAPETR 456 + G A+ ALLM R Sbjct: 419 LAGCLISAMTALLMKDSQR 437 >ZshiA [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 438 Score = 291 bits (744), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 174/446 (39%), Positives = 253/446 (56%), Gaps = 20/446 (4%) Query: 17 VSEHPTERDASNAADRANAGVGLERQRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLF 76 +S P ER S L R R AL S G ++WYDF LYG AA+VFN F Sbjct: 6 ISTRPDERTVS-----------LSRARRAAL-GSFAGAVVDWYDFLLYGITAALVFNREF 53 Query: 77 FTKLDPMIGTLAAFGSYALGFVVRPLGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGF 136 F ++ P +GTLAAF ++ +GF+ RPLGG +FGH GDR+GRK +L++T+ MMG++T LIG Sbjct: 54 FPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGI 113 Query: 137 LPTYASIGIAAPILLTLLRLIQGFAAGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALG 196 LP++++IG APILL LR IQGFA G E+GGA +S E AP NK+ +Y+S VG +G Sbjct: 114 LPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVG 173 Query: 197 VVLSAGVFTLVRQM-PEADFMNYGWRIPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKA 255 ++LS G+ +L+ M + F+++GWRIPF+ S + + +++R + ES FE+ + +A Sbjct: 174 LLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQA 233 Query: 256 VQKS--PVSAVWKHHRRHLFIAIGARFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVL 313 K PV H I R E YI +AL+Y T + + + L L Sbjct: 234 AAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGL 293 Query: 314 IGAGLGALLNPFWGMLSDRIGRKPVYILGILVMILGSWPFFMMLETRETPMIIAGFVMMI 373 + GL L P + +DR GR+ VYI G L+ L ++PFFM LE + I+ +M+ Sbjct: 294 LVGGLSCLTIPCFAWXADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLA 353 Query: 374 VFGLYPMFSVQAAFFAELFPPSVRMSGICMARELSSVITGGMAPVIATGLLAYYGGSYYP 433 + VQ F E+F S R SG + +++SV+ GG P IA L+ Y+ G+++ Sbjct: 354 NIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVXGGFTPFIAAALITYFAGNWHS 413 Query: 434 IIGYLVFMGGV---ALIALLMAPETR 456 + YL + G A+ ALLM R Sbjct: 414 VAIYL--LAGCLISAMTALLMKDNQR 437