BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0098.p (232 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0898 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4... 346 2e-95 PA3171 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-... 323 2e-88 NMA0410 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4... 289 3e-78 NMB2030 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4... 288 6e-78 VC1257 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-... 285 5e-77 >RSc0898 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1, 4-benzoquinol methylase Rso Ralstonia solanacearum Length = 260 Score = 346 bits (888), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 169/229 (73%), Positives = 195/229 (85%) Query: 2 NADPQELQKFSDLAHRWWDPTSEFRPLHEINPLRLEWINARAALSGKSVVDVGCGGGILA 61 NADP EL+KFS+LAHRWWDP SEF+PLHEINPLRL+WI + A L+GK VVDVGCGGGIL+ Sbjct: 29 NADPGELEKFSELAHRWWDPNSEFKPLHEINPLRLDWIQSTAPLAGKRVVDVGCGGGILS 88 Query: 62 ESMARKGARVTGIDLSEKALKVADLHGMESGIQVRYEKIAAEDLAAREPGQFDVVTCMEM 121 ESMAR GA V GIDLS KAL+VADLH +E+G+ V YE+IAAE LAAREPG FDVVTCMEM Sbjct: 89 ESMARAGANVKGIDLSRKALRVADLHSLEAGVAVDYEEIAAEALAAREPGSFDVVTCMEM 148 Query: 122 LEHVPDPASIVRACTAMVKPGGHVFFSTLNRNPKAYLFAIIGAEYLLRMLPKGTHDYAKF 181 LEHVPDPAS+VRAC +VKPGGHVFFST++RN KAYL A+IGAEY+L MLP+GTHDYAKF Sbjct: 149 LEHVPDPASVVRACATLVKPGGHVFFSTIHRNAKAYLLAVIGAEYVLNMLPRGTHDYAKF 208 Query: 182 ITPAELARFIREAGLELDGFKGLSYNPLTKLYSINQDTSVNYMVACRKP 230 I P+ELA F R AGLE +GL YNPLT Y++ DTSVNY++A R+P Sbjct: 209 IRPSELAGFARTAGLEPAQLRGLEYNPLTGRYALTHDTSVNYLIATRRP 257 >PA3171 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1, 4-benzoquinol methylase Pae Pseudomonas aeruginosa Length = 232 Score = 323 bits (829), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 145/228 (63%), Positives = 180/228 (78%) Query: 2 NADPQELQKFSDLAHRWWDPTSEFRPLHEINPLRLEWINARAALSGKSVVDVGCGGGILA 61 N D E+ KF LAHRWWD SEF+PLH+INPLR+ WI+ RA L+GK V+D+GCGGGIL+ Sbjct: 3 NVDHAEIAKFEALAHRWWDRESEFKPLHDINPLRVNWIDERAGLAGKKVLDIGCGGGILS 62 Query: 62 ESMARKGARVTGIDLSEKALKVADLHGMESGIQVRYEKIAAEDLAAREPGQFDVVTCMEM 121 E+MA++GA VTGID+ E L VA LH +ESG+ V Y +I AE +A PGQFDVVTC+EM Sbjct: 63 EAMAQRGASVTGIDMGEAPLAVARLHQLESGVAVDYRQITAEQMAEEMPGQFDVVTCLEM 122 Query: 122 LEHVPDPASIVRACTAMVKPGGHVFFSTLNRNPKAYLFAIIGAEYLLRMLPKGTHDYAKF 181 LEHVPDPAS++RAC +VKPGG VF ST+NRNPKAYLFA+IGAEY+L++LP+GTHD+ KF Sbjct: 123 LEHVPDPASVIRACHRLVKPGGQVFLSTINRNPKAYLFAVIGAEYILQLLPRGTHDFRKF 182 Query: 182 ITPAELARFIREAGLELDGFKGLSYNPLTKLYSINQDTSVNYMVACRK 229 I P+EL + REAGLE+ GL+YNPLTK Y + D VNYMV ++ Sbjct: 183 IRPSELGAWSREAGLEVKDIIGLTYNPLTKHYKLANDVDVNYMVQTKR 230 >NMA0410 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1, 4-benzoquinol methylase NmA Neisseria meningitidis Z2491 Length = 238 Score = 289 bits (740), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 135/229 (58%), Positives = 174/229 (75%), Gaps = 2/229 (0%) Query: 2 NADPQELQKFSDLAHRWWDPTSEFRPLHEINPLRLEWINARAALSGKSVVDVGCGGGILA 61 N D E+ KFS +A +WWD + EF+ LH+INPLRL++I+ A L GK V+DVGCGGGILA Sbjct: 7 NVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLRLDYIDGHADLCGKRVLDVGCGGGILA 66 Query: 62 ESMARKGAR-VTGIDLSEKALKVADLHGMESGI-QVRYEKIAAEDLAAREPGQFDVVTCM 119 ESMAR+GA V GID++E++L+ A LH + + + YE I EDLA EP FDVVTCM Sbjct: 67 ESMARRGAAFVKGIDMAEQSLETARLHAALNNVADIEYECIRVEDLAEAEPHSFDVVTCM 126 Query: 120 EMLEHVPDPASIVRACTAMVKPGGHVFFSTLNRNPKAYLFAIIGAEYLLRMLPKGTHDYA 179 EM+EHVPDPA+IVRAC +VKP G VFFST+N+NPK+YL I+ AEYLL+ +PKGTHD+ Sbjct: 127 EMMEHVPDPAAIVRACAKLVKPDGMVFFSTINKNPKSYLHLIVAAEYLLKFVPKGTHDWK 186 Query: 180 KFITPAELARFIREAGLELDGFKGLSYNPLTKLYSINQDTSVNYMVACR 228 KFI PAELAR R+AGL++ KG++Y+ L++ Y++ T VNYM ACR Sbjct: 187 KFIVPAELARMCRQAGLDVADTKGMTYHVLSQTYALCDSTDVNYMFACR 235 >NMB2030 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1, 4-benzoquinol methylase Nme Neisseria meningitidis MC58 Length = 242 Score = 288 bits (738), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 135/229 (58%), Positives = 174/229 (75%), Gaps = 2/229 (0%) Query: 2 NADPQELQKFSDLAHRWWDPTSEFRPLHEINPLRLEWINARAALSGKSVVDVGCGGGILA 61 N D E+ KFS +A +WWD + EF+ LH+INPLRL++I+ A L GK V+DVGCGGGILA Sbjct: 11 NVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLRLDYIDGHADLCGKRVLDVGCGGGILA 70 Query: 62 ESMARKGAR-VTGIDLSEKALKVADLHGMESGI-QVRYEKIAAEDLAAREPGQFDVVTCM 119 ESMAR+GA V GID++E++L+ A LH + + + YE I EDLA EP FDVVTCM Sbjct: 71 ESMARRGAAFVKGIDMAEQSLETARLHAALNNVADIEYECIRVEDLAEAEPHSFDVVTCM 130 Query: 120 EMLEHVPDPASIVRACTAMVKPGGHVFFSTLNRNPKAYLFAIIGAEYLLRMLPKGTHDYA 179 EM+EHVPDPA+IVRAC +VKP G VFFST+N+NPK+YL I+ AEYLL+ +PKGTHD+ Sbjct: 131 EMMEHVPDPAAIVRACANLVKPDGMVFFSTINKNPKSYLHLIVAAEYLLKFVPKGTHDWK 190 Query: 180 KFITPAELARFIREAGLELDGFKGLSYNPLTKLYSINQDTSVNYMVACR 228 KFI PAELAR R+AGL++ KG++Y+ L++ Y++ T VNYM ACR Sbjct: 191 KFIAPAELARMCRQAGLDVADTKGMTYHVLSQTYALCDSTDVNYMFACR 239 >VC1257 [H] COG2227 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1, 4-benzoquinol methylase Vch Vibrio cholerae Length = 245 Score = 285 bits (729), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 128/228 (56%), Positives = 169/228 (74%), Gaps = 1/228 (0%) Query: 2 NADPQELQKFSDLAHRWWDPTSEFRPLHEINPLRLEWINARA-ALSGKSVVDVGCGGGIL 60 N DP E++KF D+A RWWD EF+PLH+INPLRL ++ +A L GK V+DVGCGGGIL Sbjct: 16 NVDPNEIKKFEDMASRWWDLEGEFKPLHQINPLRLNYVLEKANGLFGKRVLDVGCGGGIL 75 Query: 61 AESMARKGARVTGIDLSEKALKVADLHGMESGIQVRYEKIAAEDLAAREPGQFDVVTCME 120 AESMAR+GA+VTG+D+ ++ L+VA LH +E+G ++ Y + E A P +DVVTCME Sbjct: 76 AESMAREGAQVTGLDMGKEPLEVARLHALETGTKLTYIQSTVEAHAEANPHTYDVVTCME 135 Query: 121 MLEHVPDPASIVRACTAMVKPGGHVFFSTLNRNPKAYLFAIIGAEYLLRMLPKGTHDYAK 180 MLEHVPDP S++++C +VKPGGHVFFSTLNRN K+YLFAI+GAE LL+++P+GTHD+ K Sbjct: 136 MLEHVPDPLSVIQSCAKLVKPGGHVFFSTLNRNVKSYLFAIVGAEKLLKIVPEGTHDHNK 195 Query: 181 FITPAELARFIREAGLELDGFKGLSYNPLTKLYSINQDTSVNYMVACR 228 FI P+EL + + L+ G GL YNP T Y + + VNY+V R Sbjct: 196 FIRPSELLKMVDHTALQEQGITGLHYNPFTDTYRLGSNVDVNYIVHTR 243