BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0049.p (465 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5529 [P] COG0475 Kef-type K+ transport systems, membrane compo... 480 e-135 STM0492_1 [P] COG4651 Kef-type K+ transport system, predicted NA... 156 1e-37 mlr3234_1 Non COG 154 3e-37 ECs0531_1 [P] COG4651 Kef-type K+ transport system, predicted NA... 154 4e-37 ZybaL_1 [P] COG4651 Kef-type K+ transport system, predicted NAD-... 154 6e-37 >PA5529 [P] COG0475 Kef-type K+ transport systems, membrane components Pae Pseudomonas aeruginosa Length = 585 Score = 480 bits (1235), Expect = e-135, Method: Compositional matrix adjust. Identities = 240/326 (73%), Positives = 289/326 (88%), Gaps = 1/326 (0%) Query: 1 MHAVELFIQDLAVIMLIAGVVTVVFNRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVH 60 MHAV+ FIQDLAVIML+AGVVT++F+R +QPVVLGYIVAG IIGPHTPPF LI D+ T+ Sbjct: 1 MHAVD-FIQDLAVIMLVAGVVTILFHRLRQPVVLGYIVAGFIIGPHTPPFGLIHDEDTIK 59 Query: 61 ILSELGVIFLLFSLGLEFSLKKLAKVGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAV 120 L+ELGVIFL+F LGLEFSL+KL +VGATA +AA EI+LMIWIG+EIG +F W TMD++ Sbjct: 60 TLAELGVIFLMFCLGLEFSLRKLFQVGATAFIAAFLEIVLMIWIGFEIGRFFEWSTMDSL 119 Query: 121 FLGAMLAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGIATSGSVD 180 FLGA+LA+SSTTIIVKAL +L MKNE+FAQ+IFG+LIVEDIL IG+IALLSGIA SGSV+ Sbjct: 120 FLGAILAISSTTIIVKALGDLKMKNERFAQLIFGVLIVEDILGIGIIALLSGIAVSGSVE 179 Query: 181 SGEVVNTVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCLLVMKL 240 +GEV +TVGKL LFM+V+LVVGIL+VPRLL YVA+F+SNEMLLVTVLG+ FGFCLLV+KL Sbjct: 180 TGEVFSTVGKLSLFMIVALVVGILLVPRLLAYVAKFESNEMLLVTVLGLCFGFCLLVVKL 239 Query: 241 EYSVALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYWLPIT 300 EYS+ LGAFL+GA+MAESRQ+ +IER+IE +RDMFSAIFFVAIGLL DP +L +Y PI Sbjct: 240 EYSMVLGAFLIGAIMAESRQLVQIERLIEPVRDMFSAIFFVAIGLLIDPKILVEYAWPIA 299 Query: 301 VITFAVVFGKLFSCGIGTFLAGHGGR 326 +IT AVV GK+FSCG+G F+AG+ GR Sbjct: 300 LITVAVVLGKMFSCGMGAFIAGNDGR 325 >STM0492_1 [P] COG4651 Kef-type K+ transport system, predicted NAD-binding component Sty Salmonella typhimurium LT2 Length = 412 Score = 156 bits (394), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 111/322 (34%), Positives = 181/322 (56%), Gaps = 14/322 (4%) Query: 15 MLIAGVVTVVFNRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVHILSELGVIFLLFSL 74 +++A ++ ++ N+ + ++GY++AGV+ GP TP F + D K L+ELGVI L+F + Sbjct: 15 LVLAFILGMIANKLRISPLVGYLLAGVLAGPFTPGF--VADTKLAPELAELGVILLMFGV 72 Query: 75 GLEFSLKKLAKVGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAVFLGAMLAVSSTTII 134 GL FSLK L V + A+ AVA+I + +G + GW M + G L+ +ST ++ Sbjct: 73 GLHFSLKDLMAVKSIAIPGAVAQIAVATLLGMALSAVLGWSLMTGIVFGLCLSTASTVVL 132 Query: 135 VKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGIATSGSVDSGEV------VN-- 186 ++AL E + + + QI G LIVED++ + + LL +A G V+ G+V V+ Sbjct: 133 LRALEERQLLDSQRGQIAIGWLIVEDLVMVLTLVLLPAVA--GMVEKGDVGIASLTVDMG 190 Query: 187 -TVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCLLVMKL-EYSV 244 T+GK++ F+ + ++VG +VP ++ A S E+ ++VL + G ++L + S Sbjct: 191 ITIGKVVAFIAIMMLVGRRLVPWIMARSAATGSRELFTLSVLALALGIAFGAVELFDVSF 250 Query: 245 ALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYWLPITVITF 304 ALGAF G V+ ES HR +RD F+ +FFV++G+LFDP VL + L + Sbjct: 251 ALGAFFAGMVLNESELSHRAAHDTLPLRDAFAVLFFVSVGMLFDPLVLIQQPLAVLATLA 310 Query: 305 AVVFGKLFSCGIGTFLAGHGGR 326 +VFGK + L GH R Sbjct: 311 IIVFGKSIAAFFLVRLFGHSPR 332 >mlr3234_1 Non COG Length = 429 Score = 154 bits (390), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 114/307 (37%), Positives = 164/307 (53%), Gaps = 26/307 (8%) Query: 26 NRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVHILSELGVIFLLFSLGLEFSLKKLAK 85 NRF+ P ++GY+VAGV++GP+TP F + D + L+E+GVI L+F +GL FSLK L Sbjct: 26 NRFRIPPLVGYLVAGVLVGPNTPGF--VADAGLANELAEIGVILLMFGVGLHFSLKDLLS 83 Query: 86 VGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAVFLGAMLAVSSTTIIVKALNELGMKN 145 V A AV A+ +I +G + GW + G L+V+ST ++++AL E + Sbjct: 84 VRAIAVPGAIVQIGFATALGAGLSWMLGWSMGAGLVFGLALSVASTVVLLRALQERRLIE 143 Query: 146 EKFAQIIFGILIVEDILA---------------------IGMIALLSGIATSGSVDSGEV 184 + +I G LIVED+ + + LLS A+ G G V Sbjct: 144 TERGRIAVGWLIVEDLAMVLALVLLPALAGVLGGQEQADVQLSGLLSLPASYG--IWGVV 201 Query: 185 VNTVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCLLVMKL-EYS 243 T+ K+ F+VV LVVG ++P +L+YVA S E+ ++VL I G KL S Sbjct: 202 GITLAKVAAFVVVMLVVGRRVIPWILHYVAHTGSRELFRLSVLAIALGVAFGAAKLFGVS 261 Query: 244 VALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYWLPITVIT 303 +ALGAF G +M+ES HR +RD FS +FFV++G+LFDP L LPI Sbjct: 262 LALGAFFAGMIMSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPFSLLSNGLPILATL 321 Query: 304 FAVVFGK 310 +V GK Sbjct: 322 AIIVIGK 328 >ECs0531_1 [P] COG4651 Kef-type K+ transport system, predicted NAD-binding component Ecs Escherichia coli O157:H7 Length = 413 Score = 154 bits (389), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 108/322 (33%), Positives = 181/322 (56%), Gaps = 14/322 (4%) Query: 15 MLIAGVVTVVFNRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVHILSELGVIFLLFSL 74 +++A ++ ++ N+ + ++GY++AGV+ GP TP F + D K L+ELGVI L+F + Sbjct: 15 LVLAFILGMLANKLRISPLVGYLLAGVLAGPFTPGF--VADTKLAPELAELGVILLMFGV 72 Query: 75 GLEFSLKKLAKVGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAVFLGAMLAVSSTTII 134 GL FSLK L V A A+ A+A+I + +G + GW M + G L+ +ST ++ Sbjct: 73 GLHFSLKDLMAVKAIAIPGAIAQIAVATLLGMALSAVLGWSLMTGIVFGLCLSTASTVVL 132 Query: 135 VKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGIATSGSVDSGEV------VN-- 186 ++AL E + + + QI G LIVED++ + + LL +A G ++ G+V V+ Sbjct: 133 LRALEERQLIDSQRGQIAIGWLIVEDLVMVLTLVLLPAVA--GMMEQGDVGFATLAVDMG 190 Query: 187 -TVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCLLVMKL-EYSV 244 T+GK++ F+ + ++VG +VP ++ A S E+ ++VL + G ++L + S Sbjct: 191 ITIGKVIAFIAIMMLVGRRLVPWIMARSAATGSRELFTLSVLALALGIAFGAVELFDVSF 250 Query: 245 ALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYWLPITVITF 304 ALGAF G V+ ES HR +RD F+ +FFV++G+LFDP +L + L + Sbjct: 251 ALGAFFAGMVLNESELSHRAAHDTLPLRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLA 310 Query: 305 AVVFGKLFSCGIGTFLAGHGGR 326 ++FGK + L GH R Sbjct: 311 IILFGKSLAAFFLVRLFGHSQR 332 >ZybaL_1 [P] COG4651 Kef-type K+ transport system, predicted NAD-binding component EcZ Escherichia coli O157:H7 EDL933 Length = 390 Score = 154 bits (388), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 108/322 (33%), Positives = 181/322 (56%), Gaps = 14/322 (4%) Query: 15 MLIAGVVTVVFNRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVHILSELGVIFLLFSL 74 +++A ++ ++ N+ + ++GY++AGV+ GP TP F + D K L+ELGVI L+F + Sbjct: 15 LVLAFILGMLANKLRISPLVGYLLAGVLAGPFTPGF--VADTKLAPELAELGVILLMFGV 72 Query: 75 GLEFSLKKLAKVGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAVFLGAMLAVSSTTII 134 GL FSLK L V A A+ A+A+I + +G + GW M + G L+ +ST ++ Sbjct: 73 GLHFSLKDLMAVKAIAIPGAIAQIAVATLLGMALSAVLGWSLMTGIVFGLCLSTASTVVL 132 Query: 135 VKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGIATSGSVDSGEV------VN-- 186 ++AL E + + + QI G LIVED++ + + LL +A G ++ G+V V+ Sbjct: 133 LRALEERQLIDSQRGQIAIGWLIVEDLVMVLTLVLLPAVA--GMMEQGDVGFATLAVDMG 190 Query: 187 -TVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCLLVMKL-EYSV 244 T+GK++ F+ + ++VG +VP ++ A S E+ ++VL + G ++L + S Sbjct: 191 ITIGKVIAFIAIMMLVGRRLVPWIMARSAATGSRELFTLSVLALALGIAFGAVELFDVSF 250 Query: 245 ALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYWLPITVITF 304 ALGAF G V+ ES HR +RD F+ +FFV++G+LFDP +L + L + Sbjct: 251 ALGAFFAGMVLNESELSHRAAHDTLPLRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLA 310 Query: 305 AVVFGKLFSCGIGTFLAGHGGR 326 ++FGK + L GH R Sbjct: 311 IILFGKSLAAFFLVRLFGHSQR 332