BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0066.p (364 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1446 [M] COG2230 Cyclopropane fatty acid synthase and related... 496 e-140 YPO2390 [M] COG2230 Cyclopropane fatty acid synthase and related... 400 e-111 STM1427 [M] COG2230 Cyclopropane fatty acid synthase and related... 398 e-110 Zcfa [M] COG2230 Cyclopropane fatty acid synthase and related me... 389 e-108 ECs2370 [M] COG2230 Cyclopropane fatty acid synthase and related... 389 e-108 >RSp1446 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Rso Ralstonia solanacearum Length = 388 Score = 496 bits (1278), Expect = e-140, Method: Compositional matrix adjust. Identities = 230/361 (63%), Positives = 276/361 (76%) Query: 1 LAEMLGHGGIRINGSNPWDIQLHRAGVPERAFSQANLGLGEAYMDGDWDAEQLDEFFSRL 60 + ++L +RI+G+ PWDI+LH GVP R + +LGLGEAYMDG WD LD+ F RL Sbjct: 23 IEQLLAQADVRIDGTRPWDIRLHDPGVPARVLALGSLGLGEAYMDGHWDCAHLDQLFDRL 82 Query: 61 LRTRLTDNIKPLRLVGHVLAAKFMNRQNLKRSRMVGEVHYDLGNDFYAAMLDSRMTYTCG 120 LR RL ++ + V H L A+ NRQ ++R+ VG HYDLGNDFYAAMLD+RMTYTCG Sbjct: 83 LRARLDRTVRGMSAVLHHLRARLFNRQTVRRTWQVGHTHYDLGNDFYAAMLDARMTYTCG 142 Query: 121 YWNTATTLEDAQEAKLDLICRKLQLKPGMRVLDIGCGWGSFMAFAAERYGVEAVGVSISR 180 +W TL++AQ KLDL+CRKL L+PGMRVLDIGCGWGSFM FAA+RYGV+ GV+IS Sbjct: 143 FWEGCGTLDEAQAHKLDLVCRKLGLQPGMRVLDIGCGWGSFMRFAAKRYGVQCTGVTISA 202 Query: 181 EQIALAKERYGHLPLDFRLQDYRELNERFDAIVSIGMFEHVGRRNYRTYMEVAHRCLADD 240 EQ + + LP++ RL DYR+L+ RFD IVS+GMFEHVGR+N+ YM V RCLADD Sbjct: 203 EQAGFVRAQCAGLPIETRLADYRDLDGRFDRIVSLGMFEHVGRKNHAAYMRVTERCLADD 262 Query: 241 GLFLLHTIGKNQRKSSPDPWIDKYIFPNGDLPSIGQMGDAFDHLFVVEDLHNFGADYDKT 300 GLFLLHTIG+N+ DPWID+YIFPNG+LP++ +G A + FVVEDLHNFGADYD+T Sbjct: 263 GLFLLHTIGRNESGHGTDPWIDRYIFPNGELPTLADIGLACEDRFVVEDLHNFGADYDRT 322 Query: 301 LMAWHSNFEAAWPRFEAQLGARFRRMWRYYLLSCAGAFRARDIQLWQWVLSKKGVMGGYR 360 LMAWH+NFEAAWP F LG RF RMWRYYLLSCAGAFRARD+QLWQWVLSK G G YR Sbjct: 323 LMAWHANFEAAWPAFSRTLGPRFHRMWRYYLLSCAGAFRARDLQLWQWVLSKPGRSGVYR 382 Query: 361 R 361 R Sbjct: 383 R 383 >YPO2390 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Ype Yersinia pestis Length = 383 Score = 400 bits (1027), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/364 (53%), Positives = 250/364 (68%), Gaps = 1/364 (0%) Query: 1 LAEMLGHGGIRINGSNPWDIQLHRAGVPERAFSQANLGLGEAYMDGDWDAEQLDEFFSRL 60 + +ML I INGS P+DI+++ +R +L LGE+YMDG W+ ++LD FF R+ Sbjct: 20 VQDMLNQADIEINGSRPFDIRVNNPDFFKRVLQGGSLALGESYMDGWWECDRLDIFFQRV 79 Query: 61 LRTRLTDNI-KPLRLVGHVLAAKFMNRQNLKRSRMVGEVHYDLGNDFYAAMLDSRMTYTC 119 L+ L + + + + AA+ +N Q+ +R+ +VG+ HYDLGND + +LD M Y+C Sbjct: 80 LQAGLEHKLPRHFKDTLRIAAARVINLQSKRRAWIVGKEHYDLGNDLFTQILDPNMQYSC 139 Query: 120 GYWNTATTLEDAQEAKLDLICRKLQLKPGMRVLDIGCGWGSFMAFAAERYGVEAVGVSIS 179 YW AT+LE AQE KL +IC KLQL PGM++LDIGCGWG A+AA +GV GV+IS Sbjct: 140 AYWKEATSLEQAQEHKLQMICEKLQLAPGMKLLDIGCGWGGLAAYAARHFGVSVSGVTIS 199 Query: 180 REQIALAKERYGHLPLDFRLQDYRELNERFDAIVSIGMFEHVGRRNYRTYMEVAHRCLAD 239 EQ LA++R L + LQDYR+LNE+FD IVS+GMFEHVG +NY TY +V +R L Sbjct: 200 VEQQKLAQQRCEGLDVSILLQDYRDLNEQFDRIVSVGMFEHVGPKNYSTYFKVVNRNLKP 259 Query: 240 DGLFLLHTIGKNQRKSSPDPWIDKYIFPNGDLPSIGQMGDAFDHLFVVEDLHNFGADYDK 299 DGLFLLHTIG N+ + DPWI+KYIFPNG LPS+ + A + FV+ED HNFGADYD+ Sbjct: 260 DGLFLLHTIGANRTNMNVDPWINKYIFPNGCLPSVQHIAQASEPYFVMEDWHNFGADYDR 319 Query: 300 TLMAWHSNFEAAWPRFEAQLGARFRRMWRYYLLSCAGAFRARDIQLWQWVLSKKGVMGGY 359 TLMAW+ F+AAWP A RF RM+ YYL +CAGAFRARDIQLWQ + S GV GG Sbjct: 320 TLMAWYERFQAAWPNLSANYSQRFERMFSYYLNACAGAFRARDIQLWQVLFSPNGVNGGI 379 Query: 360 RRER 363 R R Sbjct: 380 RVPR 383 >STM1427 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Sty Salmonella typhimurium LT2 Length = 382 Score = 398 bits (1022), Expect = e-110, Method: Compositional matrix adjust. Identities = 197/362 (54%), Positives = 248/362 (68%), Gaps = 2/362 (0%) Query: 3 EMLGHGGIRINGSNPWDIQLHRAGVPERAFSQANLGLGEAYMDGDWDAEQLDEFFSRLLR 62 E+L I INGS P DI++ +R + +LGLGE+YMDG W+ E+LD FFS++LR Sbjct: 22 ELLSRADITINGSAPSDIRVKNPDFFKRVLQEGSLGLGESYMDGWWECERLDIFFSKVLR 81 Query: 63 TRLTDNIKP-LRLVGHVLAAKFMNRQNLKRSRMVGEVHYDLGNDFYAAMLDSRMTYTCGY 121 L + + ++ +L A+ +N Q+ KR+ +VG+ HYDLGND ++ MLD M Y+C Y Sbjct: 82 AGLENQLPHHVKDTLRILGARLINLQSKKRAWIVGKEHYDLGNDLFSRMLDPYMQYSCAY 141 Query: 122 WNTATTLEDAQEAKLDLICRKLQLKPGMRVLDIGCGWGSFMAFAAERYGVEAVGVSISRE 181 W A TLE AQ+AKL LIC KLQL+PGMRVLDIGCGWG + A YGV VGV+IS E Sbjct: 142 WKDADTLEAAQQAKLKLICEKLQLQPGMRVLDIGCGWGGLSQYMATHYGVSVVGVTISAE 201 Query: 182 QIALAKERYGHLPLDFRLQDYRELNERFDAIVSIGMFEHVGRRNYRTYMEVAHRCLADDG 241 Q +A+ R L + L+DYR+LN++FD IVS+GMFEHVG +NY TY EV R L DG Sbjct: 202 QQKMAQTRCEGLDVSILLEDYRDLNDQFDRIVSVGMFEHVGPKNYNTYFEVVDRNLKPDG 261 Query: 242 LFLLHTIGKNQRKSSPDPWIDKYIFPNGDLPSIGQMGDAFDHLFVVEDLHNFGADYDKTL 301 LFLLHTIG + + DPWI+KYIFPNG LPS+ Q+ +A + FV+ED HNFGADYD TL Sbjct: 262 LFLLHTIGSKKTDHNVDPWINKYIFPNGCLPSVRQIAEASESHFVMEDWHNFGADYDTTL 321 Query: 302 MAWHSNFEAAWPRFEAQLGARFRRMWRYYLLSCAGAFRARDIQLWQWVLSKKGVMGGYRR 361 MAWH F AWP RF+RM+ YYL +CAGAFRARDIQLWQ V + +GV G R Sbjct: 322 MAWHERFINAWPEIAGNYNERFKRMFSYYLNACAGAFRARDIQLWQVVFT-RGVENGLRV 380 Query: 362 ER 363 R Sbjct: 381 PR 382 >Zcfa [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases EcZ Escherichia coli O157:H7 EDL933 Length = 382 Score = 389 bits (998), Expect = e-108, Method: Compositional matrix adjust. Identities = 196/366 (53%), Positives = 246/366 (67%), Gaps = 10/366 (2%) Query: 3 EMLGHGGIRINGSNPWDIQLHRAGVPERAFSQANLGLGEAYMDGDWDAEQLDEFFSRLLR 62 E+L GI INGS P DI++ +R + +LGLGE+YMDG W+ ++LD FFS++LR Sbjct: 22 ELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLR 81 Query: 63 TRLTDNIK-----PLRLVGHVLAAKFMNRQNLKRSRMVGEVHYDLGNDFYAAMLDSRMTY 117 L + + LR+ G A+ N Q+ KR+ +VG+ HYDLGND ++ MLD M Y Sbjct: 82 AGLENQLPHHFKDTLRIAG----ARLFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQY 137 Query: 118 TCGYWNTATTLEDAQEAKLDLICRKLQLKPGMRVLDIGCGWGSFMAFAAERYGVEAVGVS 177 +C YW A LE AQ+AKL +IC KLQLKPGMRVLDIGCGWG + A Y V VGV+ Sbjct: 138 SCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVT 197 Query: 178 ISREQIALAKERYGHLPLDFRLQDYRELNERFDAIVSIGMFEHVGRRNYRTYMEVAHRCL 237 IS EQ +A+ER L + LQDYR+LN++FD IVS+GMFEHVG +NY TY V R L Sbjct: 198 ISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNL 257 Query: 238 ADDGLFLLHTIGKNQRKSSPDPWIDKYIFPNGDLPSIGQMGDAFDHLFVVEDLHNFGADY 297 +G+FLLHTIG + + DPWI+KYIFPNG LPS+ Q+ + + FV+ED HNFGADY Sbjct: 258 KPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADY 317 Query: 298 DKTLMAWHSNFEAAWPRFEAQLGARFRRMWRYYLLSCAGAFRARDIQLWQWVLSKKGVMG 357 D TLMAW+ F AAWP RF+RM+ YYL +CAGAFRARDIQLWQ V S +GV Sbjct: 318 DTTLMAWYERFVAAWPEIADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFS-RGVEN 376 Query: 358 GYRRER 363 G R R Sbjct: 377 GLRVAR 382 >ECs2370 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Ecs Escherichia coli O157:H7 Length = 382 Score = 389 bits (998), Expect = e-108, Method: Compositional matrix adjust. Identities = 196/366 (53%), Positives = 246/366 (67%), Gaps = 10/366 (2%) Query: 3 EMLGHGGIRINGSNPWDIQLHRAGVPERAFSQANLGLGEAYMDGDWDAEQLDEFFSRLLR 62 E+L GI INGS P DI++ +R + +LGLGE+YMDG W+ ++LD FFS++LR Sbjct: 22 ELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLR 81 Query: 63 TRLTDNIK-----PLRLVGHVLAAKFMNRQNLKRSRMVGEVHYDLGNDFYAAMLDSRMTY 117 L + + LR+ G A+ N Q+ KR+ +VG+ HYDLGND ++ MLD M Y Sbjct: 82 AGLENQLPHHFKDTLRIAG----ARLFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQY 137 Query: 118 TCGYWNTATTLEDAQEAKLDLICRKLQLKPGMRVLDIGCGWGSFMAFAAERYGVEAVGVS 177 +C YW A LE AQ+AKL +IC KLQLKPGMRVLDIGCGWG + A Y V VGV+ Sbjct: 138 SCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVT 197 Query: 178 ISREQIALAKERYGHLPLDFRLQDYRELNERFDAIVSIGMFEHVGRRNYRTYMEVAHRCL 237 IS EQ +A+ER L + LQDYR+LN++FD IVS+GMFEHVG +NY TY V R L Sbjct: 198 ISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNL 257 Query: 238 ADDGLFLLHTIGKNQRKSSPDPWIDKYIFPNGDLPSIGQMGDAFDHLFVVEDLHNFGADY 297 +G+FLLHTIG + + DPWI+KYIFPNG LPS+ Q+ + + FV+ED HNFGADY Sbjct: 258 KPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADY 317 Query: 298 DKTLMAWHSNFEAAWPRFEAQLGARFRRMWRYYLLSCAGAFRARDIQLWQWVLSKKGVMG 357 D TLMAW+ F AAWP RF+RM+ YYL +CAGAFRARDIQLWQ V S +GV Sbjct: 318 DTTLMAWYERFVAAWPEIADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFS-RGVEN 376 Query: 358 GYRRER 363 G R R Sbjct: 377 GLRVAR 382