BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0004.p (528 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0692 [U] COG2831 Hemolysin activation/secretion protein Pae Ps... 210 6e-54 alr1659 [U] COG2831 Hemolysin activation/secretion protein Nos N... 100 1e-20 all5116 [U] COG2831 Hemolysin activation/secretion protein Nos N... 89 3e-17 RSp1535 [U] COG2831 Hemolysin activation/secretion protein Rso R... 81 5e-15 RSc3182 [U] COG2831 Hemolysin activation/secretion protein Rso R... 78 4e-14 >PA0692 [U] COG2831 Hemolysin activation/secretion protein Pae Pseudomonas aeruginosa Length = 544 Score = 210 bits (535), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 149/508 (29%), Positives = 238/508 (46%), Gaps = 25/508 (4%) Query: 35 EINRFDIAGNTLLPAADVDAAVAPFTGKDRDFSDVKGALVALKAVYQAHGYVLVRIDLPE 94 +IN + + GNT+L ++AAV PF G R D++GA AL+A YQ GY V ++LPE Sbjct: 48 DINEYVVRGNTVLDNRAIEAAVYPFLGPQRSLGDIEGARDALQAAYQEKGYQSVYVELPE 107 Query: 95 QELNNGVVVLNVVQTRIGNVAIEGNKSFTDTNIRNSLPGLQEGEAPDLNKISKSLKLANE 154 Q+++ GVV L V +T IG V + G K ++ +R +P L+EG+ PD ++ + L N Sbjct: 108 QQVSGGVVYLQVTETTIGRVRVVGAKHYSPVELREEVPALEEGKVPDFAQVQRELAQVNR 167 Query: 155 NPAKKTVMKIKPGAAADEVDASLAVTEESVWLTTLNLDNSGTEQVGRTYAGVVLQNANMF 214 P ++ + ++ G +D L V +++ W ++ L+N + + V L N++ Sbjct: 168 TPGRQVLPMVREGQRPGTMDVDLQVEDKNPWHASIGLNNDYSADTRHLRSVVSLGYDNLW 227 Query: 215 GRDHVMTLQYTTTLEHSGDVAVYGFGYHVPLYALGDSLDFFASHSDVNAGTVTAGILDIA 274 H ++L Y T + + V+ Y PL S+ SD + TV + + Sbjct: 228 QLGHAISLTYFTAPQDQDNAKVWSGSYTAPLSQRW-SVQLSGYQSDSDVATVGS----MN 282 Query: 275 VSGKGSMYGARYNQNLTTVGSYESKLVYGIDYKEVRNSMQTLGIELGNDVTVHPLSVGYV 334 V G+G YG +L G + +G+D+K+ + G + PL++GY Sbjct: 283 VVGQGHSYGVAAIYSLPVTGLWSHSFSFGVDFKDFEEQTRIGGNNDRVPIKYAPLTLGYN 342 Query: 335 GNWSMQNGVANWGATLSHNVAG-----GAKGRQQDFDAARVGAKADYTALRFAASTTQGL 389 G + G +L VAG G ++FD R AK+ + L+ + T Sbjct: 343 GFRYTERSQLALGLSL---VAGTGSLLGYGDDDEEFDRKRYRAKSGFGVLKGDLNHTLTF 399 Query: 390 PQEWLARAIVNGQYTRDALIAGEQYGAGGASSVRGFQEREVSNDSGVTANVEIYSPPLCR 449 +W Q LI+ EQ+ AGGA+SVRG+ E + D G + E +P L R Sbjct: 400 GGDWQVATKAAFQLASGPLISNEQFAAGGATSVRGYLAAENTADDGYLLSQEWRTPSLGR 459 Query: 450 N---------TDWQCRVLAFYDTAYLTRNRALPGEFRSMAIRSIGVGVRLQLRNNVSLQM 500 DW R F + A L ALP + ++ S+G+G R QL + +S + Sbjct: 460 FLGKRAGGYVNDW--RFYVFAEGAQLRLQDALPEQDDDFSLASVGIGTRAQLADWLSGSL 517 Query: 501 DYGHVLRAEATVTQRGDGRLHARLGLSF 528 D+ L E T T + D RLH + SF Sbjct: 518 DWAFPL-LEGTNTDKHDSRLHFSVQASF 544 >alr1659 [U] COG2831 Hemolysin activation/secretion protein Nos Nostoc sp. PCC 7120 Length = 504 Score = 99.8 bits (247), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 114/491 (23%), Positives = 212/491 (43%), Gaps = 34/491 (6%) Query: 25 DQPAPGSLRFEINRFDIAGNTLLPAADVDAAVAPFTGKDRDFSDVKGALVALKAVYQAHG 84 D +P F + + ++ G T+L ++ + PF + F+D+ + +Y +G Sbjct: 9 DVNSPIGESFLVKKIEVLGATVL-KNEIAKLIEPFEKRQVTFADLIQLRSDITELYIQNG 67 Query: 85 YVLVRIDLPE-QELNNGVVVLNVVQTRIGNVAIEGNKSFTDTNIRNSLPGLQEGEAP-DL 142 + LP Q L +GVV + VV+ + + I G +S +R+ L Q P + Sbjct: 68 CITSGAFLPNNQNLTDGVVKIQVVEGELEKIEITGLRSLQSVYVRSRLA--QATSTPLNR 125 Query: 143 NKISKSLKLANENPAKKTV-MKIKPGAAADEVDASLAVTEESVWLTTLNLDNSGTEQVGR 201 ++ +L+L +P + V ++ G+ A + +TE + + N+ T VG Sbjct: 126 QRLEAALQLLQLDPVIQRVNAELSAGSTAGSNILLVNITEAPAFHGGVFTANNQTPSVGS 185 Query: 202 TYAGVVLQNANMFGRDHVMTLQYTTTLEHSGDVAVYGFGYHVPLYALGDSLDFFASHSDV 261 T GV L + N+ G + +YT T + +Y Y +PL D+L F +++ Sbjct: 186 TQLGVFLNHDNLLGFGDRLAAEYTIT----EGLNLYDVSYTIPLNGNNDTLSFRVNNA-- 239 Query: 262 NAGTVTAGILDIAVSGKGSMYGARYNQNLTTVGSYESKLVYGIDYKEVRNSMQ------- 314 N+ +T D+ + + Y Y L E L G+D + + + Sbjct: 240 NSRIITDDFRDLNIRSETQTYSLSYRHPLYRQPQTELGLSLGLDLRRSQTFLLENIPFSF 299 Query: 315 TLGIELGNDVTVHPLSVGYVGNWSMQNGVANWGATLSHNVAGGAKGRQQDFDAARVGAKA 374 + G E G ++ + +W ++ A ++ GA FDA Sbjct: 300 SPGAEDGESKIT---AIRFSQDWVKRDATRVLAARSQFSIGIGA------FDATVNDTDT 350 Query: 375 DYTALRFAAST--TQGLPQEWLARAIVNGQYTRDALIAGEQYGAGGASSVRGFQEREVSN 432 D + Q L L VN Q T D+L++ E++ GG +VRG+ + ++ Sbjct: 351 DGRFFSWLGQFQWVQLLSSRTLILTRVNAQLTGDSLLSLEKFSIGGFDTVRGYTQNKLVA 410 Query: 433 DSGVTANVEIYSPPLCRNTDWQCRVLAFYDTAYLTRNRALPGEFRSMAIRSIGVGVRLQL 492 D+G TA++E+ PL N++ ++ F+D + NR G + I S+G+G+ Q Sbjct: 411 DNGFTASIEVRL-PLTANSN-ALQIAPFFDIGTVWNNRG--GNPQPQTISSLGLGLLWQP 466 Query: 493 RNNVSLQMDYG 503 +++L++DYG Sbjct: 467 SRDLNLRLDYG 477 >all5116 [U] COG2831 Hemolysin activation/secretion protein Nos Nostoc sp. PCC 7120 Length = 588 Score = 88.6 bits (218), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 103/430 (23%), Positives = 186/430 (43%), Gaps = 37/430 (8%) Query: 33 RFEINRFDIAGNTLLPAADVDAAVAPFTGKDRDFSDVKGALVALKAVYQAHGYVLVRIDL 92 +F ++R ++ G+ + P PF G++ F+++ A+ +Y +GYV + Sbjct: 95 KFRVDRIEVVGSQVFPPEKFTPITDPFVGREVSFAELLQVRDAITKLYTDNGYVTTGAVI 154 Query: 93 PEQELNNGVVVLNVVQTRIGNVAIEGNKSFTDTNIRNSLPGLQEGEAPDLNKISKSLKLA 152 Q + GV+ + +V+ + ++ I GN+ IR+ + L G+ ++ ++ + L+L Sbjct: 155 TPQTVEAGVITIQIVEGSLQDIKIVGNRRLRSQYIRDRI-QLGAGKPLNVPRLIEKLQLL 213 Query: 153 NENPAKKTV-----MKIKPGAAADEVDASLAVTEESVWLTTLNLDNSGTEQVGRTYAGVV 207 +P + + M ++PG +V+ V E + T LDN + VG GV Sbjct: 214 RLDPRIQNLSAELQMGVEPGTNILQVE----VQEADTFSLTATLDNGRSPSVGSFRRGVN 269 Query: 208 LQNANMFGRDHVMTLQYTTTLEHSGDVAVYGFGYHVPLYALGDSLDFFASHSDVNAGTVT 267 LQ AN+ G +++ Y T G A+ Y +P+ A ++ F + Sbjct: 270 LQEANLLGLGDTLSVGYNNT---DGSNAI-NLNYQLPINARNGTVSFGFNQGWNRVIEDP 325 Query: 268 AGILDIAVSGKGSMYGARYNQNLTTVGSYESKLVYGIDYKEVRNSMQTLGIELGND-VTV 326 +LDI + +G Y Q L + E L G+ + R QT ELG D + Sbjct: 326 FSVLDIQSNTTSYEFG--YRQPLIQKPTQE--LAVGLSFS--RQESQT---ELGLDNIGG 376 Query: 327 HPLSVGYVGNWSMQNGVANWGATLSHNVAGGAK---GRQQD------FDAARVGAKAD-- 375 PLS G + + ++ + T + + R Q FDA + D Sbjct: 377 FPLSPG--ADAEGRTKISAFRFTQEYTQRSNEQVFAARSQFSLGVNWFDANVNQDEPDSR 434 Query: 376 YTALRFAASTTQGLPQEWLARAIVNGQYTRDALIAGEQYGAGGASSVRGFQEREVSNDSG 435 + A R A + L + L A + Q +L+ EQ+G GG SVRG+++ + D+G Sbjct: 435 FFAWRGQAQWVRQLAPDTLFLARGDIQLASTSLVPLEQFGLGGQQSVRGYRQDTLLTDNG 494 Query: 436 VTANVEIYSP 445 + + E+ P Sbjct: 495 LLLSGELRLP 504 >RSp1535 [U] COG2831 Hemolysin activation/secretion protein Rso Ralstonia solanacearum Length = 583 Score = 81.3 bits (199), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 108/440 (24%), Positives = 174/440 (39%), Gaps = 52/440 (11%) Query: 34 FEINRFDIAGNTLLPAADVDAAVAPFTGKDRDFSDVKGALVALKAVYQAHGYVLVRIDLP 93 F+I+R ++ GNT+L A V+ PF + + + L L + AHG+V R L Sbjct: 81 FDIHRIELTGNTVLDADTVERVTQPFLNRALGTNRINLLLRRLTEAFIAHGFVTTRAYLA 140 Query: 94 EQELNNGVVVLNVVQTRIGNVAIEGNKSFTDTNIRNSLPGLQEGEAPD------------ 141 Q L GV+ + VV ++ + I G +R ++P + E P Sbjct: 141 PQNLKVGVLTIAVVPGKVEALQINGK------TVRTTVPDAKLAEGPQAGGWLTDAGTVW 194 Query: 142 -LNKISKSLKLA---------NENPAKKTVMKIKPGAAADEVDASLAVTEESVWLTTLNL 191 + + +L+L+ N + ++I PG A +LA + + Sbjct: 195 SMPAVGDTLRLSDLEQGVDQINRLRRNQAEVQILPGQAPGGSVIALANQPGDRFRFSAGT 254 Query: 192 DNSGTEQVGRTYAGVVLQNANMFGRDHVMTLQYTTTLEHSGDV--AVYGFGYHVPLYALG 249 DN G+ G T + N G ++L Y T + + + A FGY Sbjct: 255 DNYGSRATGTTRLRAGIDADNALGFQEAVSLSYIGTRDTNAAIVSAAVPFGY-------- 306 Query: 250 DSLDFFASHSDVNAGTVTAGILDIAVSGKGSMYGARYNQNLTTVGSYESKLVYGIDYKEV 309 ++ + S S+ N+ +L + ++ +++ L + +EV Sbjct: 307 NTFSYTGSLSEYNSLIGDTALLYGRTFAHAFGWNRVIERDPGGRTAFDVTLTHRRSEREV 366 Query: 310 RNSM---QTLGIELGNDVTVHPLSVGYVGNWSMQNGVANWGATLSHNVAGGAKGRQQDFD 366 N + Q+L + V V+ L VGN Q G A W A LS + D Sbjct: 367 NNLLFEPQSLSVL---RVAVNGLRKFAVGN---QGGYATWEAGLSRGM-DALNASHDAPD 419 Query: 367 AARVGAKADYTALRFAASTTQGLPQEWLA----RAIVNGQYTRDALIAGEQYGAGGASSV 422 R A + + L AST LP A R V+ Q++ AL EQ AGG SV Sbjct: 420 ITRDEAHSQFWKLDGNASTQLPLPAIGRAALAYRGQVSAQWSNVALFGSEQIFAGGMGSV 479 Query: 423 RGFQEREVSNDSGVTANVEI 442 RGF+E +S D G+T E+ Sbjct: 480 RGFREGLISGDRGLTLRNEV 499 >RSc3182 [U] COG2831 Hemolysin activation/secretion protein Rso Ralstonia solanacearum Length = 583 Score = 78.2 bits (191), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 110/451 (24%), Positives = 176/451 (39%), Gaps = 52/451 (11%) Query: 23 SVDQPAPGSLRFEINRFDIAGNTLLPAADVDAAVAPFTGKDRDFSDVKGALVALKAVYQA 82 +VD A F+I+R ++ GNT+L A V+ PF + + + L L + A Sbjct: 70 AVDAVAEPGTTFDIHRIELTGNTVLDAETVERVTQPFVNRALGTNRINLLLRRLTEAFVA 129 Query: 83 HGYVLVRIDLPEQELNNGVVVLNVVQTRIGNVAIEGNKSFTDTNIRNSLPGLQEGEAPD- 141 G+V R L Q L GV+ + VV ++ + I G +R ++P + E P Sbjct: 130 RGFVTTRAYLAPQNLKVGVLTIAVVPGKVEALQINGK------TVRTTVPDAKLAEGPQA 183 Query: 142 ------------LNKISKSLKLA---------NENPAKKTVMKIKPGAAADEVDASLAVT 180 + + +L+L+ N + ++I PG A +LA Sbjct: 184 GGWLTDAGTVWSMPAVGDTLRLSDLEQGVDQINRLRRNQAEVQILPGQAPGGSVIALANQ 243 Query: 181 EESVWLTTLNLDNSGTEQVGRTYAGVVLQNANMFGRDHVMTLQYTTTLEHSGDV--AVYG 238 + + DN G+ G T + N G ++L Y T + + + A Sbjct: 244 PGDRFRFSAGTDNYGSRATGTTRLRAGIDADNALGLQEAVSLSYIGTRDTNAAIVSAAVP 303 Query: 239 FGYHVPLYALGDSLDFFASHSDVNAGTVTAGILDIAVSGKGSMYGARYNQNLTTVGSYES 298 FGY ++ + S S+ N+ +L + ++ +++ Sbjct: 304 FGY--------NTFSYTGSLSEYNSLIGDTALLYGRTFAHAFGWNRVIERDPGGRTAFDV 355 Query: 299 KLVYGIDYKEVRNSM---QTLGIELGNDVTVHPLSVGYVGNWSMQNGVANWGATLSHNVA 355 L + +EV N + Q+L + V V+ L VGN Q G A W A LS + Sbjct: 356 TLTHRRSEREVNNLLFEPQSLSVL---RVAVNGLRKFAVGN---QGGYATWEAGLSRGM- 408 Query: 356 GGAKGRQQDFDAARVGAKADYTALRFAASTTQGLPQEWLA----RAIVNGQYTRDALIAG 411 D R A + + L AST LP A R V Q++ AL Sbjct: 409 DALNASHDAPDITRDEAHSQFWKLDGNASTQLPLPAIGRAALAYRGQVLAQWSNVALFGS 468 Query: 412 EQYGAGGASSVRGFQEREVSNDSGVTANVEI 442 EQ AGG SVRGF+E +S D G+T E+ Sbjct: 469 EQIFAGGMGSVRGFREGLISGDRGLTLRNEV 499