BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0083.p (219 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2980 [S] COG2717 Predicted membrane protein Rso Ralstonia sol... 214 1e-55 CC2747 [S] COG2717 Predicted membrane protein Ccr Caulobacter vi... 160 2e-39 BMEII0304 [S] COG2717 Predicted membrane protein Bme Brucella me... 137 2e-32 SMc01282 [S] COG2717 Predicted membrane protein Sme Sinorhizobiu... 127 2e-29 AGc3512 [S] COG2717 Predicted membrane protein Atu Agrobacterium... 122 7e-28 >RSc2980 [S] COG2717 Predicted membrane protein Rso Ralstonia solanacearum Length = 217 Score = 214 bits (544), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 109/183 (59%), Positives = 134/183 (73%) Query: 4 LRNLLFVLALLPLARLVAFGFLGRLGANPIEFITRNTGDWALYFLCITLAVTPLRKLIRW 63 +R +++LAL+P RLV G R GANP+EF+TR+TG W L LC TLAVTPLR+L Sbjct: 14 VRIAVWLLALVPFLRLVVLGATDRYGANPLEFVTRSTGTWTLVLLCCTLAVTPLRRLTGM 73 Query: 64 NWLVRLRRMLGLYAFFYVALHFTIFFWLDHFFDMQEMWADIVKRPFITVGVLAFALLIPL 123 NWL+R+RRMLGLY FFY LHF I+ +D D M DI KRPFITVG AF L+IPL Sbjct: 74 NWLIRIRRMLGLYTFFYGTLHFLIWLLVDRGLDPASMVKDIAKRPFITVGFAAFVLMIPL 133 Query: 124 AITSTNGMVRRLGGKRWQWLHRLTYVIVPLGVLHFWWVKAGKNLLAQPILFSAIVALLLL 183 A TSTN MVRRLGG+RWQWLHRL YV LG+LH+WW KAGK+ A+ +++A++A+LL Sbjct: 134 AATSTNAMVRRLGGRRWQWLHRLVYVTGVLGILHYWWHKAGKHDFAEVSIYAAVMAVLLG 193 Query: 184 ARV 186 RV Sbjct: 194 LRV 196 >CC2747 [S] COG2717 Predicted membrane protein Ccr Caulobacter vibrioides Length = 253 Score = 160 bits (406), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 89/182 (48%), Positives = 120/182 (65%), Gaps = 4/182 (2%) Query: 6 NLLFVLALLPLARLVAFGFLGRLGANPIEFITRNTGDWALYFLCITLAVTPLRKLIRWNW 65 L+++ PLA L G+ G LGANPI+ + R G+W L L + LA+TP ++++ Sbjct: 63 GLVWLACFAPLAWLAWRGYAGELGANPIDKLIRELGEWGLRLLLVGLAITPAARILKMPR 122 Query: 66 LVRLRRMLGLYAFFYVALHFTIFFWLDHFFDMQEMWADIVKRPFITVGVLAFALLIPLAI 125 LVR RR +GL+AF YVALH + +D FFD ++W DI+KRPFIT+G+L F LLIPLA+ Sbjct: 123 LVRFRRTVGLFAFAYVALHLLAYVGIDLFFDWNQLWKDILKRPFITLGMLGFMLLIPLAV 182 Query: 126 TSTNGMVRRLGGKRWQWLHRLTYVIVPLGVLHFW-WVKAGKNLLAQPILFSAIVALLLLA 184 TSTNG V R+G W LHRL Y+IVPLGV H++ VKA PI++ A+ L+L Sbjct: 183 TSTNGWVIRMGRAAWSRLHRLVYLIVPLGVAHYYLLVKADHR---PPIIYGAVFVALMLW 239 Query: 185 RV 186 RV Sbjct: 240 RV 241 >BMEII0304 [S] COG2717 Predicted membrane protein Bme Brucella melitensis Length = 220 Score = 137 bits (345), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 81/195 (41%), Positives = 119/195 (61%), Gaps = 4/195 (2%) Query: 7 LLFVLALLPLARLVAFGFLGRLGANPIEFITRNTGDWALYFLCITLAVTPLRKLIRWNWL 66 LL+ +P G G+LGA+P++ G WAL FL +TL VTP+R L L Sbjct: 22 LLYTAGFVPAVWTFYLGATGQLGADPVKTFEHLLGLWALRFLILTLLVTPMRDLTGIT-L 80 Query: 67 VRLRRMLGLYAFFYVALHFTIFFWLDHFFDMQEMWADIVKRPFITVGVLAFALLIPLAIT 126 +R RR LGL AF+Y +HFT + LD ++ + DIV+RPFIT+G+++ ALL+PLA+T Sbjct: 81 LRYRRALGLLAFYYALMHFTTYMVLDQGLNLSAIITDIVRRPFITIGMISLALLVPLALT 140 Query: 127 STNGMVRRLGGKRWQWLHRLTYVIVPLGVLHFWWVKAGKNLLAQPILFSAIVALLLLARV 186 S N +R+L G+RW LH+L Y+ + +HF + + K+ A+P++++AIVA LLL R+ Sbjct: 141 SNNWSIRKL-GRRWSSLHKLVYIAIAGSAVHF--LMSVKSWPAEPVIYAAIVAALLLWRL 197 Query: 187 LSEKNRERLAQLWSR 201 R R L R Sbjct: 198 ARPYLRTRKPALRPR 212 >SMc01282 [S] COG2717 Predicted membrane protein Sme Sinorhizobium meliloti Length = 216 Score = 127 bits (318), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 85/202 (42%), Positives = 116/202 (57%), Gaps = 8/202 (3%) Query: 8 LFVLALLPLARLVAFGFLGRLGANPIEFITRNTGDWALYFLCITLAVTPLRKLIRWNWLV 67 L++L LP G GRL N ++ G WAL FL TLA+TP+R L NWL Sbjct: 19 LYILGFLPAVWGFYLGATGRLPGNAVKEFEHLLGIWALRFLIATLAITPIRDLFGVNWL- 77 Query: 68 RLRRMLGLYAFFYVALHFTIFFWLDHFFDMQEMWADIVKRPFITVGVLAFALLIPLAITS 127 R RR LGL AF+YV +HF + LD + + ADI +RPFIT+G+ A LLIPLA+TS Sbjct: 78 RYRRALGLLAFYYVMMHFLTYMVLDQTLLLPAIVADIARRPFITIGMAALVLLIPLAVTS 137 Query: 128 TNGMVRRLGGKRWQWLHRLTYVIVPLGVLHFWWVKAGKNLLAQPILFSAIVALLLLARVL 187 +RRL G+RW LHRL YVI G LHF + K + + +L+ +VA+L+ R + Sbjct: 138 NIWSIRRL-GQRWNKLHRLVYVIAAAGALHF--AMSVKVVGPEQMLYLFLVAVLVAWRAV 194 Query: 188 SEK----NRERLAQLWSRRGAG 205 ++ R+ A + S+ AG Sbjct: 195 RKRFLRWRRQGTAPMRSQARAG 216 >AGc3512 [S] COG2717 Predicted membrane protein Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 215 Score = 122 bits (305), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 77/192 (40%), Positives = 112/192 (58%), Gaps = 9/192 (4%) Query: 8 LFVLALLPLARLVAFGFLGRLGANPIEFITRNTGDWALYFLCITLAVTPLRKLIRWNWLV 67 L+V+ L P G LG NP++ G WAL FLC+ L VTPLR L N L+ Sbjct: 22 LYVIGLCPGLWYFYLAATGGLGFNPVKDFEHLLGIWALRFLCLGLLVTPLRDLFNVN-LI 80 Query: 68 RLRRMLGLYAFFYVALHFTIFFWLDHFFDMQEMWADIVKRPFITVGVLAFALLIPLAITS 127 RR LGL AF+YV HFT++ LD + + DI+KRP+I +G+ +LIPLA+TS Sbjct: 81 AYRRALGLIAFYYVLAHFTVYLVLDRGLILGSIAGDILKRPYIMLGMAGLIILIPLALTS 140 Query: 128 TNGMVRRLGGKRWQWLHRLTYVIVPLGVLHFWWVKAGKNLLAQPILFSAIVALLLLAR-- 185 +RRL G RW LH+L Y+++ +GVLHF V A K++ +P+ + + + +LL R Sbjct: 141 NRWSIRRL-GSRWNTLHKLVYLVLIVGVLHF--VLARKSITLEPVFYISTMVVLLGYRLV 197 Query: 186 ---VLSEKNRER 194 +++ K +R Sbjct: 198 RPSIMTMKRNKR 209