BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0061.p (297 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3569 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 343 3e-94 PA0743 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 300 2e-81 AGl1448 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 265 1e-70 RSp0649 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 255 8e-68 mlr1203 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 239 5e-63 >PA3569 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Pae Pseudomonas aeruginosa Length = 298 Score = 343 bits (880), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 177/288 (61%), Positives = 215/288 (74%), Gaps = 2/288 (0%) Query: 4 DIVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVI 63 DI F+GLGNMG PMA NL+KAG+ V+ DL +V LVE G A+ A+VVI Sbjct: 3 DIAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQGAQGADSALQCCEGAEVVI 62 Query: 64 TMLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVS 123 +MLPA +HVES+YLGD G+LA + LLIDCSTIAPE+AR VA AA KG T+LDAPVS Sbjct: 63 SMLPAGQHVESLYLGDDGLLARVAGKPLLIDCSTIAPETARKVAEAAAAKGLTLLDAPVS 122 Query: 124 GGTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILM 183 GG GGA AGTL+F+VGG +GF A+P LE MG+ I+HAG G+GQ K+CNNMLLGILM Sbjct: 123 GGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGDHGAGQVAKICNNMLLGILM 182 Query: 184 IGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVD 243 GT+EA+ LG+ NG+DP VLSEVM +SSG NW L +YNP PGVM APAS GYAGGF V Sbjct: 183 AGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNLYNPWPGVMPQAPASNGYAGGFQVR 242 Query: 244 LMLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGS--GGLDFSSV 289 LM KDLGLA+ NA A +S P+GALARNL+ +H+++ + GLDFSS+ Sbjct: 243 LMNKDLGLALANAQAVQASTPLGALARNLFSLHAQADAEHEGLDFSSI 290 >PA0743 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Pae Pseudomonas aeruginosa Length = 298 Score = 300 bits (768), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 174/290 (60%), Positives = 213/290 (73%) Query: 4 DIVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVI 63 I FIGLG+MG PMA NL+KAGY ++ DLV+++VD LV +G A AV A VVI Sbjct: 3 QIAFIGLGHMGAPMATNLLKAGYLLNVFDLVQSAVDGLVAAGASAARSARDAVQGADVVI 62 Query: 64 TMLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVS 123 +MLPAS+HVE +YL D G+LA+I PGTL+++CSTIAP SAR + AAA E+G MLDAPVS Sbjct: 63 SMLPASQHVEGLYLDDDGLLAHIAPGTLVLECSTIAPTSARKIHAAARERGLAMLDAPVS 122 Query: 124 GGTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILM 183 GGT GA AGTLTFMVGG + A+P E MG+ I+HAG G+GQ KVCNN LL +LM Sbjct: 123 GGTAGAAAGTLTFMVGGDAEALEKARPLFEAMGRNIFHAGPDGAGQVAKVCNNQLLAVLM 182 Query: 184 IGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVD 243 IGT+EA+ LG+ANG++ KVL+E+M +SSG NW LEVYNP PGVMENAPAS+ Y+GGF Sbjct: 183 IGTAEAMALGVANGLEAKVLAEIMRRSSGGNWALEVYNPWPGVMENAPASRDYSGGFMAQ 242 Query: 244 LMLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNML 293 LM KDLGLA E A A+ SS PMG+LA +LY + K G DFS V + Sbjct: 243 LMAKDLGLAQEAAQASASSTPMGSLALSLYRLLLKQGYAERDFSVVQKLF 292 >AGl1448 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 304 Score = 265 bits (676), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 149/289 (51%), Positives = 179/289 (61%), Gaps = 3/289 (1%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 + F+GLG+MGLPMA NLVKAGY+V G DL +A E+G ADA A A VVIT Sbjct: 14 VAFLGLGHMGLPMAANLVKAGYAVRGFDLSEACRTAAREAGIPVAADATEATHAADVVIT 73 Query: 65 MLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSG 124 MLP +HV S+Y GD ++ + GTLLIDCST+ ESA A G +DAPVSG Sbjct: 74 MLPKGEHVVSVY-GD--LVRKVSAGTLLIDCSTVDMESALKAHELAASAGCPSVDAPVSG 130 Query: 125 GTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILMI 184 GT GA AGTLTFM GG D F A+P LE MGK I H G G+GQ K+CNNM+LGI MI Sbjct: 131 GTSGAAAGTLTFMCGGEPDAFSAARPVLEAMGKKIVHCGKGGNGQAAKICNNMILGISMI 190 Query: 185 GTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVDL 244 SEA LG G+ + L +V S SSG+ W+L Y P PG + +PA+K Y GF L Sbjct: 191 AVSEAFVLGEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANKDYQPGFAAAL 250 Query: 245 MLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNML 293 MLKDL L+ A TG S P+GA A LY+ G GG DFS++ ML Sbjct: 251 MLKDLKLSQAAANGTGVSTPLGAAASALYESFVGEGGGGRDFSAIVEML 299 >RSp0649 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Rso Ralstonia solanacearum Length = 298 Score = 255 bits (652), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 154/292 (52%), Positives = 195/292 (66%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 I FIGLGNMG PMA+NL+KAG+++S DL +V LV++G AA A IT Sbjct: 3 IAFIGLGNMGAPMARNLLKAGHALSVFDLDARAVQSLVDAGASAAPSPKAAATGADCAIT 62 Query: 65 MLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSG 124 MLPA+ HV S+ D GILA I G +ID STI P+SA+ +AA A G +DAPVSG Sbjct: 63 MLPAAAHVHSVLTADDGILAGIAKGVPIIDSSTIDPDSAKALAALATAHGNPFVDAPVSG 122 Query: 125 GTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILMI 184 GTGGA AGTLTFMVGG F +P L MGK + H G +G+GQ K+CNN++LGI M Sbjct: 123 GTGGAAAGTLTFMVGGGAAVFEQVRPVLSAMGKNLVHCGEAGAGQGAKICNNLVLGITMA 182 Query: 185 GTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVDL 244 G +EA+ LG A G+DPKVL +++ S+GR W+ + YNP PGV+E AP+S+GY GGFG DL Sbjct: 183 GVAEAMSLGEALGIDPKVLGGIINTSTGRCWSSDTYNPFPGVIETAPSSRGYTGGFGTDL 242 Query: 245 MLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNMLAKA 296 MLKDLGLA + A + V +GALA+ LY S G+G LDFS+V + A Sbjct: 243 MLKDLGLAGDAAKSVRQPVYLGALAQQLYQASSSQGAGKLDFSAVIRLYRPA 294 >mlr1203 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Mlo Mesorhizobium loti Length = 294 Score = 239 bits (611), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 147/289 (50%), Positives = 180/289 (62%), Gaps = 3/289 (1%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 I FIGLGNMG PMA NLVKAG+ V G DL+ ++ E G V A+A AAV A VVIT Sbjct: 4 IAFIGLGNMGNPMAANLVKAGHQVHGFDLMPENLTVAREHGVVVMANAPAAVKDADVVIT 63 Query: 65 MLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSG 124 MLPA KHV S+Y + I K L ID STI ESAR A A + G +DAPVSG Sbjct: 64 MLPAGKHVLSVY---EDIAPKAKKDALFIDSSTIDVESARKAHAIAAKHGLLSIDAPVSG 120 Query: 125 GTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILMI 184 GTGGA AGTLTFM GG++D F A+P L+ M I H G G+GQ K+CNNM+LGI MI Sbjct: 121 GTGGAAAGTLTFMAGGSNDAFAAAEPVLKPMAGRIVHCGGDGAGQAAKICNNMILGISMI 180 Query: 185 GTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVDL 244 G +EA L G+ + L +V S SSG+ W+L Y P PG + +PA++ Y GF L Sbjct: 181 GVAEAFVLAEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANRDYRPGFAAAL 240 Query: 245 MLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNML 293 MLKDL L+ E A + G+ P+GA A LY + + G GG DFS + N L Sbjct: 241 MLKDLKLSQEAAQSAGAVTPLGAEAAQLYALFNAQGHGGADFSGIINFL 289