BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0025.p (214 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1378 [E] COG1446 Asparaginase Rso Ralstonia solanacearum 258 1e-68 STM0847 [E] COG1446 Asparaginase Sty Salmonella typhimurium LT2 233 2e-61 ybiK [E] COG1446 Asparaginase Eco Escherichia coli K12 232 5e-61 Z1051m [E] COG1446 Asparaginase EcZ Escherichia coli O157:H7 EDL933 229 3e-60 ECs0907 [E] COG1446 Asparaginase Ecs Escherichia coli O157:H7 219 4e-57 >RSc1378 [E] COG1446 Asparaginase Rso Ralstonia solanacearum Length = 320 Score = 258 bits (658), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 136/215 (63%), Positives = 160/215 (74%), Gaps = 9/215 (4%) Query: 1 MENSAHVLLAGCGADAFARQQGLAIVDPTYFHTDARHQQWLRVRGQSGM-MLDHDAAAFT 59 ME+S HVL GA+AFA QGL V+P Y++T+AR+ QW R R +GM +LDHDAA+ Sbjct: 114 MEHSEHVLFTSEGAEAFAASQGLEFVEPDYYYTEARYAQWQRARQHAGMALLDHDAASLL 173 Query: 60 FKESAHGTAPVDPIDPDNKFGTVGAVALDMHGNVAAATSTGGITNKQAGRVGDTPLIGAG 119 + AP +PIDPD+KFGTVGAVA D G +AAATSTGG+TNKQ GRVGDTPLIGAG Sbjct: 174 AQ------AP-EPIDPDSKFGTVGAVACDAQGRLAAATSTGGVTNKQVGRVGDTPLIGAG 226 Query: 120 CYAANATAAISATGTGEVFIRTVAAYDVAARMAYAGATLQEAMEQVVMQVLPAYEGRGGL 179 CYA N AA+S TGTGE+FIR VAAYDVAA+M YAG L EA + VVM+ L A GRGGL Sbjct: 227 CYA-NRAAAVSCTGTGEMFIRAVAAYDVAAQMEYAGKPLAEACDDVVMRKLVAIGGRGGL 285 Query: 180 IAVDAQGNLELSFNTEGMYRGYARGGDTPVTAIYK 214 IAVDA N+ L FNTEGMYRG+ARG P +IY+ Sbjct: 286 IAVDAHSNVALPFNTEGMYRGFARGDAAPEVSIYR 320 >STM0847 [E] COG1446 Asparaginase Sty Salmonella typhimurium LT2 Length = 313 Score = 233 bits (595), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 122/214 (57%), Positives = 145/214 (67%), Gaps = 15/214 (7%) Query: 1 MENSAHVLLAGCGADAFARQQGLAIVDPTYFHTDARHQQWLRVRGQSGMMLDHDAAAFTF 60 ME S HVL+ G GA+ FA QG+A V P F T AR++Q L R M LDH A Sbjct: 114 MERSPHVLMVGEGAENFAFSQGMARVSPDIFSTPARYEQLLAARAAGEMALDHSGA---- 169 Query: 61 KESAHGTAPVDPIDPDNKFGTVGAVALDMHGNVAAATSTGGITNKQAGRVGDTPLIGAGC 120 P+D K GTVGAVA D GN+AAATSTGG+TNK GRVGD+PL+GAGC Sbjct: 170 -----------PLDETKKMGTVGAVARDKFGNLAAATSTGGMTNKLPGRVGDSPLVGAGC 218 Query: 121 YAANATAAISATGTGEVFIRTVAAYDVAARMAYAGATLQEAMEQVVMQVLPAYEGRGGLI 180 YA NA+ A+S TGTGEVFIRT+AAYD+AA M Y G +L +A E+VVM+ LPA G GGLI Sbjct: 219 YANNASVAVSCTGTGEVFIRTLAAYDIAALMEYGGLSLADACERVVMEKLPALGGSGGLI 278 Query: 181 AVDAQGNLELSFNTEGMYRGYARGGDTPVTAIYK 214 AVD +GN+ L FN+EGMYR + GDTP T IY+ Sbjct: 279 AVDHEGNVALPFNSEGMYRAWGYAGDTPTTGIYR 312 >ybiK [E] COG1446 Asparaginase Eco Escherichia coli K12 Length = 321 Score = 232 bits (591), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 118/214 (55%), Positives = 145/214 (67%), Gaps = 15/214 (7%) Query: 1 MENSAHVLLAGCGADAFARQQGLAIVDPTYFHTDARHQQWLRVRGQSGMMLDHDAAAFTF 60 ME S HV++ G GA+ FA +G+ V P F T R++Q L R + +LDH A Sbjct: 114 MEQSPHVMMIGEGAENFAFARGMERVSPEIFSTSLRYEQLLAARKEGATVLDHSGA---- 169 Query: 61 KESAHGTAPVDPIDPDNKFGTVGAVALDMHGNVAAATSTGGITNKQAGRVGDTPLIGAGC 120 P+D K GTVGAVALD+ GN+AAATSTGG+TNK GRVGD+PL+GAGC Sbjct: 170 -----------PLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC 218 Query: 121 YAANATAAISATGTGEVFIRTVAAYDVAARMAYAGATLQEAMEQVVMQVLPAYEGRGGLI 180 YA NA+ A+S TGTGEVFIR +AAYD+AA M Y G +L EA E+VVM+ LPA G GGLI Sbjct: 219 YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLI 278 Query: 181 AVDAQGNLELSFNTEGMYRGYARGGDTPVTAIYK 214 A+D +GN+ L FNTEGMYR + GDTP T IY+ Sbjct: 279 AIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYR 312 >Z1051m [E] COG1446 Asparaginase EcZ Escherichia coli O157:H7 EDL933 Length = 321 Score = 229 bits (585), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 117/214 (54%), Positives = 144/214 (67%), Gaps = 15/214 (7%) Query: 1 MENSAHVLLAGCGADAFARQQGLAIVDPTYFHTDARHQQWLRVRGQSGMMLDHDAAAFTF 60 ME S HV++ G GA+ FA G+ V P F T R++Q + R + +LDH A Sbjct: 114 MEXSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEGATVLDHSGA---- 169 Query: 61 KESAHGTAPVDPIDPDNKFGTVGAVALDMHGNVAAATSTGGITNKQAGRVGDTPLIGAGC 120 P+D K GTVGAVALD+ GN+AAATSTGG+TNK GRVGD+PL+GAGC Sbjct: 170 -----------PLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC 218 Query: 121 YAANATAAISATGTGEVFIRTVAAYDVAARMAYAGATLQEAMEQVVMQVLPAYEGRGGLI 180 YA NA+ A+S TGTGEVFIR +AAYD+AA M Y G +L EA E+VVM+ LPA G GGLI Sbjct: 219 YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLI 278 Query: 181 AVDAQGNLELSFNTEGMYRGYARGGDTPVTAIYK 214 A+D +GN+ L FNTEGMYR + GDTP T IY+ Sbjct: 279 AIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYR 312 >ECs0907 [E] COG1446 Asparaginase Ecs Escherichia coli O157:H7 Length = 202 Score = 219 bits (557), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 112/208 (53%), Positives = 140/208 (67%), Gaps = 15/208 (7%) Query: 7 VLLAGCGADAFARQQGLAIVDPTYFHTDARHQQWLRVRGQSGMMLDHDAAAFTFKESAHG 66 +++ G GA+ FA G+ V P F T R++Q + R + +LDH A Sbjct: 1 MMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEGATVLDHSGA---------- 50 Query: 67 TAPVDPIDPDNKFGTVGAVALDMHGNVAAATSTGGITNKQAGRVGDTPLIGAGCYAANAT 126 P+D K GTVGAVALD+ GN+AAATSTGG+TNK GRVGD+PL+GAGCYA NA+ Sbjct: 51 -----PLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNAS 105 Query: 127 AAISATGTGEVFIRTVAAYDVAARMAYAGATLQEAMEQVVMQVLPAYEGRGGLIAVDAQG 186 A+S TGTGEVFIR +AAYD+AA M Y G +L EA E+VVM+ LPA G GGLIA+D +G Sbjct: 106 VAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEG 165 Query: 187 NLELSFNTEGMYRGYARGGDTPVTAIYK 214 N+ L FNTEGMYR + GDTP T IY+ Sbjct: 166 NVALPFNTEGMYRAWGYAGDTPTTGIYR 193