BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0230.p (485 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1614 [GEPR] COG0477 Permeases of the major facilitator superf... 147 4e-35 STM1543 [GEPR] COG0477 Permeases of the major facilitator superf... 140 1e-32 ygcZ [GEPR] COG0477 Permeases of the major facilitator superfami... 136 9e-32 STM2962 [GEPR] COG0477 Permeases of the major facilitator superf... 136 1e-31 Z4105 [GEPR] COG0477 Permeases of the major facilitator superfam... 135 3e-31 >RSp1614 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 448 Score = 147 bits (372), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 95/356 (26%), Positives = 179/356 (50%), Gaps = 18/356 (5%) Query: 3 YCGLLWALATVLTGFAGGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGIT 62 + L W+LAT +TG A G SL+ R+ LG+GE +P+ SRW K +R I Sbjct: 86 FAVLWWSLATAVTGLATGFKSLVGLRVALGVGEAGAYPSNAGITSRWFPKQERATVAAIF 145 Query: 63 HAFARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRITAEE 122 + +++G+A+A + W++ W+ +F + G+ +VW +VWA VF + PA+H + A E Sbjct: 146 DSGSKLGSAIALPLIAWLLVTFDWKITFTVAGSLGIVWCVVWALVFRDSPAEHEGVNAAE 205 Query: 123 L----EVLPPGK-KTSPKIPWGPLF--KRMMPVTIVYFCYGWTLWLFLSWIPQYFLHSYD 175 L + LPP + +P +PW L + + + I +F + + F++W+P Y + Sbjct: 206 LAHIQKGLPPARGADAPAMPWTKLLTHRNVWAMCIGFFMINYNSYFFITWLPTYLVKERG 265 Query: 176 LDIKKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVSVCMLLTLLSLLPL 235 + + + AS F + + G SD++ +G L + + + + L + S + L Sbjct: 266 MGLMEMGFMASLPLFVSMFVEVFAGWASDRVYA-SGRLSLTATRKLFLVIGLVMASSIGL 324 Query: 236 -MFTHNLYVSIISLSAGFFFAEMTIGPMWAIPMDIAP-EYSGTASGMMNTGSALAAIISP 293 F + V++I L + +WA+P D+AP +G+ N+ S + ++ P Sbjct: 325 AAFAQSAVVAVILLCIAKSGTTVAASQVWALPADVAPGNTVSMVAGLQNSVSNMGGVVGP 384 Query: 294 VLSGYLIDKTGNWELPFLGSMVLMGFGVL----LAFRMQPDSKFENTAPDAGGINP 345 +++G ++ TG++ + S L+G +L L +++P + FE P+ +NP Sbjct: 385 IVTGAIVGATGSFIPALVFSAALIGLAILNYLFLLGKVEPIA-FE---PNPATLNP 436 >STM1543 [GEPR] COG0477 Permeases of the major facilitator superfamily Sty Salmonella typhimurium LT2 Length = 428 Score = 140 bits (352), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 94/317 (29%), Positives = 162/317 (51%), Gaps = 15/317 (4%) Query: 6 LLWALATVLTGFAGGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAF 65 LLW++ LTG GL ++L+ R+L G+ EG A ++ W+++ + A GI A Sbjct: 82 LLWSVFCGLTGLVTGLWTMLIVRVLFGMAEGPVSAAGNKIINNWISRKESATAIGIFSAG 141 Query: 66 ARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRITAEE--- 122 + +G AV+ V + GWR +F I F LVWV++W F+ ++ P R+ EE Sbjct: 142 SPLGGAVSGPIVGLLALSLGWRPAFGIIFLFGLVWVLLWYFIVSDKPTMSKRLAPEERID 201 Query: 123 LE-----VLPPGKKTSPKIPWGPLFKRMM--PVTIVYFCYGWTLWLFLSWIPQYFLHSYD 175 E +L + +P + G K+ M T+ +F Y + L+ FL+W P Y HS Sbjct: 202 FENHEDVILSDDGRATPSL--GYYMKQPMVWATTLAFFSYNYILFFFLTWFPSYLNHSLH 259 Query: 176 LDIKKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVSVCMLLTLLSLLPL 235 LDIK+ ++ + G +G LGG+ SD I R TGN +R ++ VC+ + + Sbjct: 260 LDIKEISIATVIPWVIGAIGMVLGGVCSDVIYRITGNALLSRRLILGVCLAGAAVCVAVS 319 Query: 236 MFTHNLYVSIISLSAGFFFAEMTIGPM-WAIPMDIA-PEYSGTASGMMNTGSALAAIISP 293 + +I +S F +T GP+ WA+ D+ + G+ G M+ + ++ II P Sbjct: 320 GTVSTIGSAITLMSVSLFLLYLT-GPIYWAVIQDVVHKDKVGSVGGAMHGLANISGIIGP 378 Query: 294 VLSGYLIDKTGNWELPF 310 +++G+++ +G ++ F Sbjct: 379 LVTGFIVQFSGKYDYAF 395 >ygcZ [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 450 Score = 136 bits (343), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 99/345 (28%), Positives = 167/345 (48%), Gaps = 35/345 (10%) Query: 6 LLWALATVLTGFA------GGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQ 59 +W++ T+L GF G +V+L R L+GL E +FP + ++ W +RG A Sbjct: 93 FIWSMFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAV 152 Query: 60 GITHAFARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRIT 119 I ++ + + W+ E GW F+ G +V +W V E P +HP + Sbjct: 153 SIFNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHE-PNQHPGVN 211 Query: 120 AEELEVLPPG--------KKTSPKIP----WGPL-----FKRMMPVTIVYFCYGWTLWLF 162 +ELE + G + T K+P WG + + M+ V I +C + F Sbjct: 212 KKELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFF 271 Query: 163 LSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVS 222 ++W P Y + + + I K+ AS G +G LGGI+SD ++R TG+L AR + Sbjct: 272 ITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIV 331 Query: 223 VCMLLTLLSLLPLMFTHNLYVS--IISLSAGFFFAEMTIGPMWAIPMDIAP-EYSGTASG 279 + MLL+++ ++F + + V II A FF + WA+ D AP E SG + G Sbjct: 332 MGMLLSMV----MVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGG 387 Query: 280 MMNTGSALAAIISPVLSGYLIDKTGNWELPFLGSMVLMGFGVLLA 324 + N ++ I++P+ GY++ TG+ F G+++ +G L+A Sbjct: 388 LFNMFGNISGIVTPIAIGYIVGTTGS----FNGALIYVGVHALIA 428 >STM2962 [GEPR] COG0477 Permeases of the major facilitator superfamily Sty Salmonella typhimurium LT2 Length = 452 Score = 136 bits (342), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 99/345 (28%), Positives = 166/345 (48%), Gaps = 35/345 (10%) Query: 6 LLWALATVLTGFA------GGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQ 59 +W++ T+L GF G +V+L R L+GL E +FP + ++ W +RG A Sbjct: 93 FIWSVFTLLQGFVDIFSGFGIIVALFTLRFLVGLAESPSFPGNSRIVAAWFPAQERGTAV 152 Query: 60 GITHAFARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRIT 119 I ++ + + W+ E GW F+ G ++ +W V E P HP + Sbjct: 153 AIFNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIIISFIWLKVIHE-PNNHPGVN 211 Query: 120 AEELEVLPPG--------KKTSPKIP----WGPL-----FKRMMPVTIVYFCYGWTLWLF 162 +ELE + G K T K+P WG + + M+ V I +C + F Sbjct: 212 KKELEYIAEGGALINMDQKDTKAKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFF 271 Query: 163 LSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVS 222 ++W P Y + + + I K+ AS G +G LGGI+SD ++R TG+L AR + Sbjct: 272 ITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIV 331 Query: 223 VCMLLTLLSLLPLMFTHNLYVS--IISLSAGFFFAEMTIGPMWAIPMDIAP-EYSGTASG 279 + MLL+++ ++F + + V II A FF + WA+ D AP E SG + G Sbjct: 332 LGMLLSMV----MVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGG 387 Query: 280 MMNTGSALAAIISPVLSGYLIDKTGNWELPFLGSMVLMGFGVLLA 324 + N ++ I++P+ GY++ TG+ F G+++ +G L+A Sbjct: 388 LFNMFGNISGIVTPIAIGYIVGTTGS----FNGALIYVGVHALVA 428 >Z4105 [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 450 Score = 135 bits (339), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 98/345 (28%), Positives = 166/345 (48%), Gaps = 35/345 (10%) Query: 6 LLWALATVLTGFA------GGLVSLLLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQ 59 +W++ T+L GF G +V+L R L+GL E +FP + ++ W +RG A Sbjct: 93 FIWSMFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAV 152 Query: 60 GITHAFARIGNAVAPAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPAKHPRIT 119 I ++ + + W+ E GW F+ G + +W V E P +HP + Sbjct: 153 SIFNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHE-PNQHPGVN 211 Query: 120 AEELEVLPPG--------KKTSPKIP----WGPL-----FKRMMPVTIVYFCYGWTLWLF 162 +ELE + G + T K+P WG + + M+ V I +C + F Sbjct: 212 QKELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFF 271 Query: 163 LSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGDTLGGIVSDKILRNTGNLKRARSYMVS 222 ++W P Y + + + I K+ AS G +G LGGI+SD ++R TG+L AR + Sbjct: 272 ITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIV 331 Query: 223 VCMLLTLLSLLPLMFTHNLYVS--IISLSAGFFFAEMTIGPMWAIPMDIAP-EYSGTASG 279 + MLL+++ ++F + + V II A FF + WA+ D AP E SG + G Sbjct: 332 MGMLLSMV----MVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGG 387 Query: 280 MMNTGSALAAIISPVLSGYLIDKTGNWELPFLGSMVLMGFGVLLA 324 + N ++ I++P+ GY++ TG+ F G+++ +G L+A Sbjct: 388 LFNMFGNISGIVTPIAIGYIVGTTGS----FNGALIYVGVHALIA 428