BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0140.p (432 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2337 [J] COG0172 Seryl-tRNA synthetase Rso Ralstonia solanace... 689 0.0 NMB1684 [J] COG0172 Seryl-tRNA synthetase Nme Neisseria meningit... 626 e-179 NMA1943 [J] COG0172 Seryl-tRNA synthetase NmA Neisseria meningit... 617 e-176 PA2612 [J] COG0172 Seryl-tRNA synthetase Pae Pseudomonas aeruginosa 566 e-161 STM0963 [J] COG0172 Seryl-tRNA synthetase Sty Salmonella typhimu... 565 e-161 >RSc2337 [J] COG0172 Seryl-tRNA synthetase Rso Ralstonia solanacearum Length = 438 Score = 689 bits (1777), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/438 (76%), Positives = 372/438 (84%), Gaps = 8/438 (1%) Query: 1 MIDIQLLRKDIDNVAARLAARKFQLDVAAFNALEGERKQIQTRTEELQGKRNSLSKQVGM 60 M+DIQLLRKDID VAARL R ++LDVA F ALE ERK IQTRTEELQ +RNSLSKQ+GM Sbjct: 1 MLDIQLLRKDIDAVAARLNDRGYELDVAGFAALEAERKAIQTRTEELQARRNSLSKQIGM 60 Query: 61 LKGKGEDASAVMAEVSGIGDELKASAARLEEVQVQLSDFLLTLPNLPHESVPAGADESGN 120 LKGKGEDASAVMAEV+GIGDELKASAA+L+ VQ +L D LL++PN+PHESVPAG E+ N Sbjct: 61 LKGKGEDASAVMAEVAGIGDELKASAAQLDVVQGRLQDLLLSIPNVPHESVPAGKSEAEN 120 Query: 121 VELRKVGTPRTFDFEVKDHVDVGAALGLDFDVATKLTGSRFAVMKGGIARLHRALAQFML 180 VE+R+ GTPR FDF VKDHVD+GA LGLDFDVA KL+GSRFAV+KG +ARLHRALAQFML Sbjct: 121 VEVRREGTPRAFDFPVKDHVDLGAGLGLDFDVAAKLSGSRFAVLKGPVARLHRALAQFML 180 Query: 181 DTHTLEHGYTECYTPYIVNADSLRGTGQLPKFEADLF-AAKKGGQ-----EGDSSDNAAL 234 DTHT EHGYTE Y PYIVNA S+RGTGQLPKFE DLF +K GQ G+ +N Sbjct: 181 DTHTQEHGYTEAYVPYIVNAASMRGTGQLPKFEEDLFRVPRKMGQATEAEAGEHVEN--F 238 Query: 235 YLIPTAEVPLTNLVRDEIVAGDTLPLKMTAHSPCFRSEAGSYGRDTRGMIRQHQFDKVEM 294 YLIPTAEVPLTNLVRDEIVAGD LP+K AHSPCFRSEAGSYG+DTRGMIRQHQFDKVEM Sbjct: 239 YLIPTAEVPLTNLVRDEIVAGDALPMKFAAHSPCFRSEAGSYGKDTRGMIRQHQFDKVEM 298 Query: 295 VQIVHPEKSYEALDEMLGHAENILKKLGLPYRVITLCTGDMGFGAAKTFDIEVWLPAQNT 354 VQIV PE S+EALD M HAENIL+KL LP+R + LCTGDMGFG+ KT+DIEVW+PAQNT Sbjct: 299 VQIVQPETSFEALDAMTHHAENILRKLELPFRTVVLCTGDMGFGSTKTYDIEVWIPAQNT 358 Query: 355 YREISSVSNCEAFQARRMQARFRNSQGKPELVHTLNGSGLAVGRTLVAVLENGQQADGSV 414 YREISS SN FQARRMQARFRN+QGKPEL+HTLNGSGLAVGRTLVAVLEN Q ADGSV Sbjct: 359 YREISSCSNMGDFQARRMQARFRNAQGKPELLHTLNGSGLAVGRTLVAVLENYQNADGSV 418 Query: 415 TIPEVLRPYMGGLEKLAP 432 T+P LRPY+GG L P Sbjct: 419 TVPAALRPYLGGQAVLKP 436 >NMB1684 [J] COG0172 Seryl-tRNA synthetase Nme Neisseria meningitidis MC58 Length = 431 Score = 626 bits (1614), Expect = e-179, Method: Compositional matrix adjust. Identities = 299/432 (69%), Positives = 351/432 (81%), Gaps = 6/432 (1%) Query: 1 MIDIQLLRKDIDNVAARLAARKFQLDVAAFNALEGERKQIQTRTEELQGKRNSLSKQVGM 60 M+DIQLLR + VA RLA R + D A F+ LE RK +Q +TEELQ RNS+SKQ+G Sbjct: 1 MLDIQLLRSNTAAVAERLARRGYDFDTARFDTLEERRKSVQVKTEELQASRNSISKQIGA 60 Query: 61 LKGKG--EDASAVMAEVSGIGDELKASAARLEEVQVQLSDFLLTLPNLPHESVPAGADES 118 LKG+G E+A A M +V+ I +L+ +AA L+ VQ +L +LL++PNLPHESVPAG DE+ Sbjct: 61 LKGQGKHEEAQAAMNQVAQIKTDLEQAAADLDAVQKELDAWLLSIPNLPHESVPAGKDET 120 Query: 119 GNVELRKVGTPRTFDFEVKDHVDVGAALGLDFDVATKLTGSRFAVMKGGIARLHRALAQF 178 NVE+RKVGTPR FDFE+KDHVD+G LGLDF+ KL+G+RF VM+G IARLHRALAQF Sbjct: 121 ENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSGARFTVMRGQIARLHRALAQF 180 Query: 179 MLDTHTLEHGYTECYTPYIVNADSLRGTGQLPKFEADLFAAKKGGQEGDSSDNAALYLIP 238 MLDTHTL+HGYTE YTPYIV+ +L+GTGQLPKF DLF +GG E ++ YLIP Sbjct: 181 MLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDLFHVTRGGDETKTTQ----YLIP 236 Query: 239 TAEVPLTNLVRDEIVAGDTLPLKMTAHSPCFRSEAGSYGRDTRGMIRQHQFDKVEMVQIV 298 TAEV LTN V D I+ + LPLK+TAHSPCFRSEAGSYG+DTRG+IRQHQFDKVEMVQIV Sbjct: 237 TAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGKDTRGLIRQHQFDKVEMVQIV 296 Query: 299 HPEKSYEALDEMLGHAENILKKLGLPYRVITLCTGDMGFGAAKTFDIEVWLPAQNTYREI 358 HPEKSYE L+EM+GHAENILK L LPYRVITLCTGDMGFGAAKT+D+EVW+PAQNTYREI Sbjct: 297 HPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMGFGAAKTYDLEVWVPAQNTYREI 356 Query: 359 SSVSNCEAFQARRMQARFRNSQGKPELVHTLNGSGLAVGRTLVAVLENGQQADGSVTIPE 418 SS SNCE FQARR++ARF++ GK LVHTLNGSGLAVGRTLVAVLEN Q ADGS+ IP Sbjct: 357 SSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGLAVGRTLVAVLENHQNADGSINIPA 416 Query: 419 VLRPYMGGLEKL 430 L+PYMGG+ KL Sbjct: 417 ALQPYMGGVAKL 428 >NMA1943 [J] COG0172 Seryl-tRNA synthetase NmA Neisseria meningitidis Z2491 Length = 431 Score = 617 bits (1592), Expect = e-176, Method: Compositional matrix adjust. Identities = 296/432 (68%), Positives = 347/432 (80%), Gaps = 6/432 (1%) Query: 1 MIDIQLLRKDIDNVAARLAARKFQLDVAAFNALEGERKQIQTRTEELQGKRNSLSKQVGM 60 M+DIQLLR + VA RLA R + D A F+ALE RK +Q +TEELQ RNS+SKQ+G Sbjct: 1 MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKTEELQASRNSISKQIGA 60 Query: 61 LKGKG--EDASAVMAEVSGIGDELKASAARLEEVQVQLSDFLLTLPNLPHESVPAGADES 118 LKG+G E+A M +V+ I +L+ +AA L+ VQ +L +LL++PNLPHE VP G DE+ Sbjct: 61 LKGQGKHEEAQVAMDQVAQIKTDLEQAAADLDAVQKELDAWLLSIPNLPHEDVPFGKDET 120 Query: 119 GNVELRKVGTPRTFDFEVKDHVDVGAALGLDFDVATKLTGSRFAVMKGGIARLHRALAQF 178 NVE+RKVGTPR FDFE+KDHVD+G LGLDF+ KL+G+RF VM+G IARLHRALAQF Sbjct: 121 ENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSGARFTVMRGQIARLHRALAQF 180 Query: 179 MLDTHTLEHGYTECYTPYIVNADSLRGTGQLPKFEADLFAAKKGGQEGDSSDNAALYLIP 238 MLDTHTL+HGYTE YTPYIV+ +L+GTGQLPKF DLF +GG E ++ YLIP Sbjct: 181 MLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDLFHVTRGGDESKTTQ----YLIP 236 Query: 239 TAEVPLTNLVRDEIVAGDTLPLKMTAHSPCFRSEAGSYGRDTRGMIRQHQFDKVEMVQIV 298 TAEV LTN V I+ + LPLK+TAHSPCFRSEAGSYG+DTRG+IRQHQFDKVEMVQIV Sbjct: 237 TAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGKDTRGLIRQHQFDKVEMVQIV 296 Query: 299 HPEKSYEALDEMLGHAENILKKLGLPYRVITLCTGDMGFGAAKTFDIEVWLPAQNTYREI 358 HPEKSYE L+EM+GHAENILK L LPYRVITLCTGDMGFGA KT+D+EVW+PAQNTYREI Sbjct: 297 HPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMGFGATKTYDLEVWVPAQNTYREI 356 Query: 359 SSVSNCEAFQARRMQARFRNSQGKPELVHTLNGSGLAVGRTLVAVLENGQQADGSVTIPE 418 SS SNCE FQARRM+ARF++ GK LVHTLNGSGLAVGRTLVAVLEN Q ADGS+ IP Sbjct: 357 SSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGLAVGRTLVAVLENHQNADGSINIPA 416 Query: 419 VLRPYMGGLEKL 430 L+PYMGG+ KL Sbjct: 417 ALQPYMGGVAKL 428 >PA2612 [J] COG0172 Seryl-tRNA synthetase Pae Pseudomonas aeruginosa Length = 426 Score = 566 bits (1459), Expect = e-161, Method: Compositional matrix adjust. Identities = 278/430 (64%), Positives = 338/430 (78%), Gaps = 9/430 (2%) Query: 1 MIDIQLLRKDIDNVAARLAARKFQLDVAAFNALEGERKQIQTRTEELQGKRNSLSKQVGM 60 M+D +L+R VAARLA R FQLDVA ALE +RK +QTRTE+LQ +RN+ SK +G Sbjct: 1 MLDPKLVRTQPQEVAARLATRGFQLDVARIEALEEQRKSVQTRTEQLQAERNARSKAIGQ 60 Query: 61 LKGKGEDASAVMAEVSGIGDELKASAARLEEVQVQLSDFLLTLPNLPHESVPAGADESGN 120 K +GED + ++A+V +G EL+ +L+ +Q +L LL +PNLPHESVP GADE N Sbjct: 61 AKQRGEDIAPLLADVDRMGSELEEGKRQLDAIQGELDAMLLGIPNLPHESVPVGADEDAN 120 Query: 121 VELRKVGTPRTFDFEVKDHVDVGAALG-LDFDVATKLTGSRFAVMKGGIARLHRALAQFM 179 VE+R+ GTP+TFDFEVKDHV +G G LDF+ A KL+G+RFA+M+G IARLHRALAQFM Sbjct: 121 VEVRRWGTPKTFDFEVKDHVALGERHGWLDFETAAKLSGARFALMRGPIARLHRALAQFM 180 Query: 180 LDTHTLEHGYTECYTPYIVNADSLRGTGQLPKFEADLFAAKKGGQEGDSSDNAALYLIPT 239 ++ HT EHGY E YTPY+V A +L+GTGQLPKFE DLF K G++G+ A LYLIPT Sbjct: 181 INLHTAEHGYEEAYTPYLVQAPALQGTGQLPKFEEDLF---KIGRDGE----ADLYLIPT 233 Query: 240 AEVPLTNLVRDEIVAGDTLPLKMTAHSPCFRSEAGSYGRDTRGMIRQHQFDKVEMVQIVH 299 AEV LTN+V +I+ LPLK AH+PCFRSEAG+ GRDTRGMIRQHQFDKVEMVQIV Sbjct: 234 AEVSLTNIVSGQILDAKQLPLKFVAHTPCFRSEAGASGRDTRGMIRQHQFDKVEMVQIVD 293 Query: 300 PEKSYEALDEMLGHAENILKKLGLPYRVITLCTGDMGFGAAKTFDIEVWLPAQNTYREIS 359 P SYEAL+ + +AE +L+ L LPYRV+ LCTGDMGFGA KT+D+EVW+P+Q+ YREIS Sbjct: 294 PATSYEALEGLTANAERVLQLLELPYRVLALCTGDMGFGATKTYDLEVWVPSQDKYREIS 353 Query: 360 SVSNCEAFQARRMQARFRNSQ-GKPELVHTLNGSGLAVGRTLVAVLENGQQADGSVTIPE 418 S SNC FQARRMQAR+RN + GKPELVHTLNGSGLAVGRTLVAVLEN QQADGS+ +PE Sbjct: 354 SCSNCGDFQARRMQARYRNPETGKPELVHTLNGSGLAVGRTLVAVLENYQQADGSIRVPE 413 Query: 419 VLRPYMGGLE 428 VL+PYM G+E Sbjct: 414 VLKPYMAGIE 423 >STM0963 [J] COG0172 Seryl-tRNA synthetase Sty Salmonella typhimurium LT2 Length = 430 Score = 565 bits (1456), Expect = e-161, Method: Compositional matrix adjust. Identities = 279/432 (64%), Positives = 328/432 (75%), Gaps = 5/432 (1%) Query: 1 MIDIQLLRKDIDNVAARLAARKFQLDVAAFNALEGERKQIQTRTEELQGKRNSLSKQVGM 60 M+D LLR + D VA +LA R F+LDV ALE RK +Q TE LQ +RNS SK +G Sbjct: 1 MLDPNLLRNEPDAVAEKLARRGFKLDVDKLRALEERRKVLQVNTENLQAERNSRSKSIGQ 60 Query: 61 LKGKGEDASAVMAEVSGIGDELKASAARLEEVQVQLSDFLLTLPNLPHESVPAGADESGN 120 K +GED + EV+ +G+EL A+ A L+ + ++ D LT+PNLP + VP G DE+ N Sbjct: 61 AKARGEDIEPLRLEVNKLGEELDAAKAELDTLLAEIRDIALTIPNLPADEVPVGKDENDN 120 Query: 121 VELRKVGTPRTFDFEVKDHVDVGAA-LGLDFDVATKLTGSRFAVMKGGIARLHRALAQFM 179 VE+ + GTPR FDFE++DHV +G GLDF A KLTGSRF VMKG IAR+HRAL+QFM Sbjct: 121 VEVSRWGTPREFDFEIRDHVTLGEMHSGLDFAAAVKLTGSRFVVMKGQIARMHRALSQFM 180 Query: 180 LDTHTLEHGYTECYTPYIVNADSLRGTGQLPKFEADLFAAKKGGQEGDSSDNAALYLIPT 239 LD HT +HGY+E Y PY+VN D+L GTGQLPKF DLF + +E DSS+ A LIPT Sbjct: 181 LDLHTEQHGYSENYVPYLVNHDTLYGTGQLPKFAGDLFHTRPLEEEADSSNYA---LIPT 237 Query: 240 AEVPLTNLVRDEIVAGDTLPLKMTAHSPCFRSEAGSYGRDTRGMIRQHQFDKVEMVQIVH 299 AEVPLTNLVRDEI+ D LP+KMTAH+PCFRSEAGSYGRDTRG+IR HQFDKVEMVQIV Sbjct: 238 AEVPLTNLVRDEIIDEDQLPIKMTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVEMVQIVR 297 Query: 300 PEKSYEALDEMLGHAENILKKLGLPYRVITLCTGDMGFGAAKTFDIEVWLPAQNTYREIS 359 PE S AL+EM GHAE +L+ LGLPYR I LCTGDMGFGA KT+D+EVW+PAQNTYREIS Sbjct: 298 PEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMGFGACKTYDLEVWVPAQNTYREIS 357 Query: 360 SVSNCEAFQARRMQARFRN-SQGKPELVHTLNGSGLAVGRTLVAVLENGQQADGSVTIPE 418 S SN FQARRMQAR R+ S K LVHTLNGSGLAVGRTLVAV+EN QQADG + +PE Sbjct: 358 SCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSGLAVGRTLVAVMENYQQADGRIEVPE 417 Query: 419 VLRPYMGGLEKL 430 VLRPYM GLE + Sbjct: 418 VLRPYMNGLEYI 429