BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0105.p (218 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR2595 [IQR] COG1028 Dehydrogenases with different specificities... 159 4e-39 CC0398 [IQR] COG1028 Dehydrogenases with different specificities... 150 2e-36 alr1722 [IQR] COG1028 Dehydrogenases with different specificitie... 138 9e-33 ML0315 [IQR] COG1028 Dehydrogenases with different specificities... 131 1e-30 Rv2263 [IQR] COG1028 Dehydrogenases with different specificities... 128 1e-29 >DR2595 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Dra Deinococcus radiodurans Length = 336 Score = 159 bits (402), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 88/192 (45%), Positives = 121/192 (63%), Gaps = 5/192 (2%) Query: 12 EVLSGMDLRGKRVFITGVSAGLGVETARALVAHGADVIGTVRDLDKAHGATKVVRDAAER 71 EV+ G+DL+GK +TG ++GLG ETARAL+ GA VI VRD K +V + + Sbjct: 34 EVVRGVDLKGKTAVVTGGASGLGTETARALLLAGAHVILPVRDRAKGE---RVAAELRQS 90 Query: 72 SGSSFEIVNMDLADLASVRAVSDTMAAAQKPFDLVVANAGIMATPFGKTKDGFELQFGTN 131 +G + E+V++DL LASVR + + +++ NAG+MATP +T DGFE QFGTN Sbjct: 91 TGGTVELVDLDLGSLASVRRGAAEIRQLAPRIHILINNAGVMATPQSRTVDGFETQFGTN 150 Query: 132 VLGHYVLINR--LAPLIREGGRVIVLSSNGHRFADVDLDDPNFEHTPYDPWVAYGRSKTG 189 LGH++L A + RV+ L+S+GHR +D+ DD NFE PYDPW AYG+SKT Sbjct: 151 HLGHFLLTRELLPALMAAAPARVVALTSSGHRRSDIVWDDLNFERRPYDPWDAYGQSKTA 210 Query: 190 DALLAVAFDARH 201 +AL AV R+ Sbjct: 211 NALFAVGLTQRY 222 >CC0398 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Ccr Caulobacter vibrioides Length = 323 Score = 150 bits (378), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 85/217 (39%), Positives = 124/217 (57%), Gaps = 12/217 (5%) Query: 4 FDATSTTDEVLSGMDLRGKRVFITGVSAGLGVETARALVAHGADVIGTVRDLDKAHGATK 63 F A ST EV++G DL G+ +TG + G+GVETARAL GA+VI R + Sbjct: 9 FGAKSTAREVVAGHDLSGRVAIVTGAATGIGVETARALALAGAEVIIAARKPELGEEVAN 68 Query: 64 VVRDAAERSGSSFEIVNMDLADLASVRAVSDTMAAAQKPFDLVVANAGIMATPFGKTKDG 123 + + A SF + +DL+ L ++R + + D+++ NA +MA+P +T DG Sbjct: 69 AINEEAGSKRVSFGM--LDLSSLEAIRHFVNVWG--DRRIDILINNAAVMASPLMRTADG 124 Query: 124 FELQFGTNVLGHYVLINRLAPLIREGG-------RVIVLSSNGHRFADVDLDDPNFEHTP 176 FE+QFGTN LGH++L LAP + G R++ LSS GHR + + DDPN+ P Sbjct: 125 FEMQFGTNHLGHFLLSVLLAPNLIAGAKASGKPSRLVSLSSIGHRRSGIHFDDPNYTTRP 184 Query: 177 YDPWVAYGRSKTGDALLAVAFDARHRHRD-HAAXCAP 212 Y+ W AYG++KT ++L AV FD R + + HA P Sbjct: 185 YEKWEAYGQAKTANSLFAVGFDKRFKDQGVHANAVMP 221 >alr1722 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Nos Nostoc sp. PCC 7120 Length = 311 Score = 138 bits (348), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 74/200 (37%), Positives = 111/200 (55%), Gaps = 6/200 (3%) Query: 20 RGKRVFITGVSAGLGVETARALVAHGADVIGTVRDLDKAHGATKVVRDAAERSGSSFEIV 79 +G+ +TG S+G+G ETAR L A VI VR+LDK + A + + + + +++ Sbjct: 15 KGRLAIVTGSSSGIGYETARVLANKQASVIIAVRNLDKGNKA--LAKILQQNKDADVKLM 72 Query: 80 NMDLADLASVRAVSDTMAAAQKPFDLVVANAGIMATPFGKTKDGFELQFGTNVLGHYVLI 139 +DLA+LASV+ ++ DL++ NAG+M P+ KT DGFELQFGTN LGH+ L Sbjct: 73 ELDLANLASVKNFAENFRKNYLRLDLLINNAGVMIPPYSKTTDGFELQFGTNHLGHFALT 132 Query: 140 NRLAPLI--REGGRVIVLSSNGHRFADVDLDDPNFEHTPYDPWVAYGRSKTGDALLAVAF 197 +L + EG R++ +SS H +D DD N+E Y W AYG SK + L + Sbjct: 133 GQLLEFLISTEGSRIVNVSSGAHNMGKIDFDDLNWEQRSYAKWKAYGDSKLAN--LYFTY 190 Query: 198 DARHRHRDHAAXCAPPASTP 217 + + +D+ AS P Sbjct: 191 ELDRKLKDNGIDTLVTASHP 210 >ML0315 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mle Mycobacterium leprae Length = 304 Score = 131 bits (329), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 80/188 (42%), Positives = 109/188 (57%), Gaps = 10/188 (5%) Query: 18 DLRGKRVFITGVSAGLGVETARALVAHGADVIGTVRDLDKAHGATKVVRDAAERSGSSFE 77 D G+ ITG + GLG +TA AL HGA V+ VR+LDK G R A + ++ Sbjct: 11 DQTGRVAVITGANTGLGYQTALALAEHGAHVVLAVRNLDK--GKDAAARITATSAQNNVA 68 Query: 78 IVNMDLADLASVRAVSDTMAAAQKPFDLVVANAGIMATPFGKTKDGFELQFGTNVLGHY- 136 + +DLA L SVRA + + + DL++ NAG+M TP TKDGFELQFGTN LGH+ Sbjct: 69 LQELDLASLESVRAAAKQLRSDYDHIDLLINNAGVMWTPKSTTKDGFELQFGTNHLGHFA 128 Query: 137 ---VLINRLAPLIREGGRVIVLSSNGHR-FADVDLDDPNFEHTPYDPWVAYGRSKTGDAL 192 +L++RL P++ G RVI +SS HR FAD+ +D +E Y+ AYG+SK + L Sbjct: 129 FTGLLLDRLLPIV--GSRVITVSSLSHRLFADIHFNDLQWE-CNYNRVAAYGQSKLANLL 185 Query: 193 LAVAFDAR 200 R Sbjct: 186 FTYELQRR 193 >Rv2263 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mtu Mycobacterium tuberculosis H37Rv Length = 317 Score = 128 bits (321), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 82/195 (42%), Positives = 108/195 (55%), Gaps = 6/195 (3%) Query: 11 DEVLSGMDLRGKRVFITGVSAGLGVETARALVAHGADVIGTVRDLDKAHGATKVVRDAAE 70 D V + DL GK ITG ++GLG AR L A GADVI +R+ K A + +R A Sbjct: 4 DLVATVPDLSGKLAIITGANSGLGFGLARRLSAAGADVIMAIRNRAKGEAAVEEIRTAV- 62 Query: 71 RSGSSFEIVNMDLADLASVRAVSDTMAAAQKPFDLVVANAGIMATPFG-KTKDGFELQFG 129 + I +DL+ LASV A+ + + A +P DL++ NAG+M P T DGFELQFG Sbjct: 63 -PDAKLTIKALDLSSLASVAALGEQLMADGRPIDLLINNAGVMTPPERVTTADGFELQFG 121 Query: 130 TNVLGHYVLINRLAPLIR--EGGRVIVLSSNGHRFADVDLDDPNFEHTPYDPWVAYGRSK 187 +N LGH+ L L PL+R + RV+ LSS R + DD FE + Y P AYG+SK Sbjct: 122 SNHLGHFALTAHLLPLLRAAQRARVVSLSSLAARRGRIHFDDLQFERS-YAPMTAYGQSK 180 Query: 188 TGDALLAVAFDARHR 202 + A D R R Sbjct: 181 LAVLMFARELDRRSR 195