BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0082.p (323 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1678 [R] COG2252 Permeases Rso Ralstonia solanacearum 434 e-121 SMb21281 [R] COG2252 Permeases Sme Sinorhizobium meliloti 357 1e-98 PA4719 [R] COG2252 Permeases Pae Pseudomonas aeruginosa 357 2e-98 VC2278 [R] COG2252 Permeases Vch Vibrio cholerae 346 4e-95 NMA2056 [R] COG2252 Permeases NmA Neisseria meningitidis Z2491 340 2e-93 >RSp1678 [R] COG2252 Permeases Rso Ralstonia solanacearum Length = 434 Score = 434 bits (1115), Expect = e-121, Method: Compositional matrix adjust. Identities = 209/287 (72%), Positives = 253/287 (88%) Query: 1 MTFLDKFFKLSDNGTTVRTELLAGLTTFLTMAYIIFVNPSILGDAGMPKDSVFVATCVAA 60 M++L +FFKL ++ T VRTE++AGLTTFLTMAYI+FVNP+IL DAG+P D+VFVATCVAA Sbjct: 1 MSWLARFFKLEEHQTDVRTEVVAGLTTFLTMAYIVFVNPNILADAGVPHDAVFVATCVAA 60 Query: 61 AIGTLIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAVFISGCLFLLVSLFR 120 AIGT IMGLYANYPI +APGMGLNAYFAY+VVKGMGF WQAALGAVF+SGCLF+LVSLFR Sbjct: 61 AIGTAIMGLYANYPIAMAPGMGLNAYFAYSVVKGMGFTWQAALGAVFVSGCLFMLVSLFR 120 Query: 121 IRELIINAIPHSLRTAIPAGIGLFLALISLKNAGIVAASPATFVTMGDLHQAAPVLAIIG 180 IRE+IIN IPHS+R AI AGIGLFL ++SL+ AG+V +PAT V +GD+HQ + +LA+IG Sbjct: 121 IREMIINGIPHSIRIAITAGIGLFLGIVSLRGAGLVVGNPATLVALGDVHQPSVILAVIG 180 Query: 181 FLVIVALDQLKIRGALLIGILTVTVLSFIFGGNHFNGVFSMPPSISPTLFQLDLKGAISM 240 F +IV LD L+++GA+LIGIL VTV SF GN F+GV SMPP I+PTL QLD++GA+S+ Sbjct: 181 FFLIVTLDHLRVKGAILIGILAVTVASFFVAGNTFHGVVSMPPPIAPTLLQLDIQGALSV 240 Query: 241 GLLNVVLVFFLVELFDATGTLMGVAQRAGLVKNGKIERINKALLADS 287 G+LNVVLVFFLVELFDATGTLMGVA RAGL+K G+++R+NKALLADS Sbjct: 241 GILNVVLVFFLVELFDATGTLMGVANRAGLLKAGRMDRLNKALLADS 287 >SMb21281 [R] COG2252 Permeases Sme Sinorhizobium meliloti Length = 457 Score = 357 bits (917), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 174/283 (61%), Positives = 225/283 (79%) Query: 5 DKFFKLSDNGTTVRTELLAGLTTFLTMAYIIFVNPSILGDAGMPKDSVFVATCVAAAIGT 64 ++ FKL ++GTTVRTE++AG+TTFLTM+YIIFVNP IL GM ++++FVATC+AAA+G+ Sbjct: 30 ERLFKLKEHGTTVRTEVIAGVTTFLTMSYIIFVNPDILSTTGMDRNAIFVATCLAAALGS 89 Query: 65 LIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAVFISGCLFLLVSLFRIREL 124 +M L AN+PIG+APGMGLNA+FA+ VV +GF WQ ALGAVFISG +FL +++ +R Sbjct: 90 AVMALVANWPIGMAPGMGLNAFFAFTVVAALGFTWQQALGAVFISGIIFLFLTVTGVRSW 149 Query: 125 IINAIPHSLRTAIPAGIGLFLALISLKNAGIVAASPATFVTMGDLHQAAPVLAIIGFLVI 184 +I IPHSLR+AI GIGLFL +I+LKNAGIV +PAT V +GDL Q P+LAI+GF VI Sbjct: 150 LIAGIPHSLRSAIATGIGLFLGIIALKNAGIVVDNPATLVGLGDLKQTGPLLAILGFFVI 209 Query: 185 VALDQLKIRGALLIGILTVTVLSFIFGGNHFNGVFSMPPSISPTLFQLDLKGAISMGLLN 244 LD L +RG++LIGIL VTVLS G + F G+ S PPSI+PT QLD+ GA+ GL++ Sbjct: 210 AVLDALNVRGSILIGILVVTVLSMFLGVSEFQGIVSAPPSIAPTFLQLDIMGALHGGLVH 269 Query: 245 VVLVFFLVELFDATGTLMGVAQRAGLVKNGKIERINKALLADS 287 V+LVF LVE+FDATGTL+GVA+RA LV+ GK R+ +ALLADS Sbjct: 270 VILVFVLVEVFDATGTLIGVAKRAKLVEEGKPSRLGRALLADS 312 >PA4719 [R] COG2252 Permeases Pae Pseudomonas aeruginosa Length = 431 Score = 357 bits (915), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 179/285 (62%), Positives = 229/285 (80%), Gaps = 1/285 (0%) Query: 4 LDKFFKLSDNGTTVRTELLAGLTTFLTMAYIIFVNPSILGDAGMPKDSVFVATCVAAAIG 63 L+K F+L + TTVRTE+LAG+TTFLTMAYI+FVNPSILG+ GM K +VFVATC+AAAIG Sbjct: 2 LEKLFQLKAHNTTVRTEILAGITTFLTMAYILFVNPSILGETGMDKGAVFVATCLAAAIG 61 Query: 64 TLIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAVFISGCLFLLVSLFRIRE 123 + IMGL ANYPI LAPGMGLNA+F Y VV MG WQ ALGAVFIS +F L+S+FRIRE Sbjct: 62 STIMGLIANYPIALAPGMGLNAFFTYTVVLHMGHSWQVALGAVFISATMFFLLSIFRIRE 121 Query: 124 LIINAIPHSLRTAIPAGIGLFLALISLKNAGIVAASPATFVTMGDLHQAAPVLAIIGFLV 183 IIN+IP LR+AI AGIGLFLALI+L+NAGIV +PAT + MGDL + AP+LA +GF++ Sbjct: 122 WIINSIPLPLRSAIAAGIGLFLALIALQNAGIVVDNPATLIGMGDLTKPAPILATLGFIL 181 Query: 184 IVALDQLKIRGALLIGILTVTVLSFIFGGNHFNGVFSMPPSISPTLFQLDLKGAISMGLL 243 IVAL+ + GA+LIGIL VT ++ + G F GV SMPPS++PT QLD+KGA+ +GL+ Sbjct: 182 IVALEARSVTGAVLIGILVVTAIAILLGVTQFGGVVSMPPSLAPTFLQLDIKGALDIGLV 241 Query: 244 NVVLVFFLVELFDATGTLMGVAQRAGLV-KNGKIERINKALLADS 287 +V+ F V+LFD +GTL+GVA+RAGL+ K+G + ++ +AL+ADS Sbjct: 242 SVIFAFLFVDLFDNSGTLIGVAKRAGLMGKDGHMPKMGRALIADS 286 >VC2278 [R] COG2252 Permeases Vch Vibrio cholerae Length = 430 Score = 346 bits (888), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 175/285 (61%), Positives = 215/285 (75%), Gaps = 1/285 (0%) Query: 4 LDKFFKLSDNGTTVRTELLAGLTTFLTMAYIIFVNPSILGDAGMPKDSVFVATCVAAAIG 63 L+K FKLS+ GT VRTE+LAG+TTFLTMAYIIFVNP+IL DAGM + +VFVATC+AAAIG Sbjct: 2 LEKLFKLSEYGTNVRTEILAGVTTFLTMAYIIFVNPAILSDAGMDRGAVFVATCLAAAIG 61 Query: 64 TLIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAVFISGCLFLLVSLFRIRE 123 IMG ANYPI APGMGLNA+F Y VV GMG WQ AL AVF SG LF+L+SLF+IRE Sbjct: 62 CFIMGFIANYPIAQAPGMGLNAFFTYGVVLGMGHTWQVALAAVFCSGVLFILLSLFKIRE 121 Query: 124 LIINAIPHSLRTAIPAGIGLFLALISLKNAGIVAASPATFVTMGDLHQAAPVLAIIGFLV 183 IIN+IPHSLRT I AGIGLFLA I+LKNAGIV +PAT V++G + VLA +GF + Sbjct: 122 WIINSIPHSLRTGISAGIGLFLAFIALKNAGIVVDNPATLVSLGAITSLHAVLAAVGFFL 181 Query: 184 IVALDQLKIRGALLIGILTVTVLSFIFGGNHFNGVFSMPPSISPTLFQLDLKGAISMGLL 243 + L ++GA++I IL VT L +FG + G+ S PPSI+PT QLD +G++ Sbjct: 182 TIGLVYRGVKGAVMIAILAVTALGLVFGDVQWGGIMSTPPSIAPTFMQLDFSAVFEIGMI 241 Query: 244 NVVLVFFLVELFDATGTLMGVAQRAGLV-KNGKIERINKALLADS 287 +VV F V+LFD GTL+GVA +AGL+ K+GKI R+N+ALLADS Sbjct: 242 SVVFAFLFVDLFDTAGTLVGVATKAGLIEKDGKIPRLNRALLADS 286 >NMA2056 [R] COG2252 Permeases NmA Neisseria meningitidis Z2491 Length = 436 Score = 340 bits (872), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 171/286 (59%), Positives = 226/286 (79%) Query: 2 TFLDKFFKLSDNGTTVRTELLAGLTTFLTMAYIIFVNPSILGDAGMPKDSVFVATCVAAA 61 T LD FKL NGTTVRTEL+AGLTTFLTM YI+ VNP ILG+ GM +VFVATC+A+A Sbjct: 7 TLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNPLILGETGMDMGAVFVATCIASA 66 Query: 62 IGTLIMGLYANYPIGLAPGMGLNAYFAYAVVKGMGFPWQAALGAVFISGCLFLLVSLFRI 121 IG +MG NYPI LAPGMGLNAYF +AVVKGMG PWQ ALGAVFISG +F+L S F++ Sbjct: 67 IGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGVPWQVALGAVFISGLIFILFSFFKV 126 Query: 122 RELIINAIPHSLRTAIPAGIGLFLALISLKNAGIVAASPATFVTMGDLHQAAPVLAIIGF 181 RE+++NA+P L+ +I AGIGLFLALISLK AGI+ A+PAT V +GD+HQ + +LA+ GF Sbjct: 127 REMLVNALPMGLKMSIAAGIGLFLALISLKGAGIIVANPATLVGLGDIHQPSALLALFGF 186 Query: 182 LVIVALDQLKIRGALLIGILTVTVLSFIFGGNHFNGVFSMPPSISPTLFQLDLKGAISMG 241 ++V L +++GA++I ILT+TV++ + G N F+G+ PSI+PT Q+D KG ++ Sbjct: 187 AMVVVLGHFRVQGAIIITILTITVIASLMGLNEFHGIIGEVPSIAPTFMQMDFKGLFTVS 246 Query: 242 LLNVVLVFFLVELFDATGTLMGVAQRAGLVKNGKIERINKALLADS 287 +++V+ VFFLV+LFD+TGTL+GV+ RAGL+ +GK+ R+ +ALLADS Sbjct: 247 MVSVIFVFFLVDLFDSTGTLVGVSHRAGLLVDGKLPRLKRALLADS 292