BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0068.p (280 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1147 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 274 1e-73 PA1012 [R] COG1235 Metal-dependent hydrolases of the beta-lactam... 190 2e-48 BH4023 [R] COG1235 Metal-dependent hydrolases of the beta-lactam... 139 6e-33 lin0319 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 138 1e-32 CAC3538 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 137 4e-32 >RSc1147 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Rso Ralstonia solanacearum Length = 261 Score = 274 bits (701), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 129/229 (56%), Positives = 168/229 (73%), Gaps = 1/229 (0%) Query: 1 LKFASLGSGSEGNALIISGTSGATTTTVMLDCGFGIKETERRLTRIGKLPQDINGIVVTH 60 ++FASLGSGS+GNAL++ T G T+T ++LDCGFGI+ET RRL R+G P ++ I+VTH Sbjct: 3 VRFASLGSGSDGNALLVESTDGTTSTRILLDCGFGIRETVRRLERLGCAPASLDAILVTH 62 Query: 61 EHQDHVGGVFKFARRHRIPVWLTYGTFQAVGE-EACKNVQLHFCRDSEAVVIGDLSLMPY 119 EH DH+G + FA ++ +PVW ++GT++A + FCR +A IGDL ++PY Sbjct: 63 EHGDHIGSAYAFAAKYSVPVWTSHGTYRATASLRGADRAAVRFCRADDAFSIGDLRILPY 122 Query: 120 TVPHDAREPVQYVAGDGKRKLGVLTDAGQSTTHLVQALGGCDALVLECNHDRQMLADSAY 179 TVPHDAREP+Q++ DG R+LGVLTDAG ST +L L G A VLECNHDR+MLA+SAY Sbjct: 123 TVPHDAREPLQFIFDDGARRLGVLTDAGMSTEYLCGHLTGLHAFVLECNHDREMLANSAY 182 Query: 180 PPSLKRRIGGAYGHLANDTSAEILAALDKQKLKTVIGAHLSLKNNTPGL 228 P SLK+RIGG +GHLAND +A ILA + L TV+ AHLS +NNTP L Sbjct: 183 PWSLKQRIGGDFGHLANDIAAAILARVRHDGLHTVVAAHLSKQNNTPEL 231 >PA1012 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Pae Pseudomonas aeruginosa Length = 252 Score = 190 bits (483), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 100/230 (43%), Positives = 146/230 (63%), Gaps = 7/230 (3%) Query: 1 LKFASLGSGSEGNALIISGTSGATTTTVMLDCGFGIKETERRLTRIGKLPQDINGIVVTH 60 ++FA LGSGS GN+ ++S ++ T +++DCGF ++E +RL R+G P ++ I+VTH Sbjct: 1 MRFAVLGSGSRGNSALVS----SSDTRILIDCGFPLREIIKRLARLGLSPAQLDAILVTH 56 Query: 61 EHQDHVGGVFKFARRHRIPVWLTYGTFQAVGEEACKNVQLHFCRDSEAVVIGDLSLMPYT 120 EH DH+ GV AR ++IPV+L+ GT + V+L R + + I L + Sbjct: 57 EHADHIHGVELLARHYQIPVYLSAGTLSGM-RRPVHPVEL--LRAGQRLAIRGLEVQVVG 113 Query: 121 VPHDAREPVQYVAGDGKRKLGVLTDAGQSTTHLVQALGGCDALVLECNHDRQMLADSAYP 180 V HDAREP Q+V DG+R+ G+LTD G +T +++ DALV+E NHD MLA YP Sbjct: 114 VSHDAREPTQFVLSDGQRRFGMLTDLGVATAEVLECYRAVDALVVEANHDSTMLAGGPYP 173 Query: 181 PSLKRRIGGAYGHLANDTSAEILAALDKQKLKTVIGAHLSLKNNTPGLAR 230 LK R+GG+ GHL N SAE++ LD +++ ++ AHLS KNN+P LAR Sbjct: 174 YFLKARVGGSRGHLNNHQSAELVKQLDWGRMQHLVLAHLSEKNNSPQLAR 223 >BH4023 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Bha Bacillus halodurans Length = 264 Score = 139 bits (350), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 84/240 (35%), Positives = 126/240 (52%), Gaps = 10/240 (4%) Query: 1 LKFASLGSGSEGNALIISGTSGATTTTVMLDCGFGIKETERRLTRIGKLPQDINGIVVTH 60 L+F+ L SGS GNAL + +++D G K+ + +I P +NGI+VTH Sbjct: 3 LRFSVLASGSTGNALYVE----TDKQKLLVDAGLSGKKMDELFQKIDCDPSQLNGILVTH 58 Query: 61 EHQDHVGGVFKFARRHRIPVWLTYGTFQAVGEEAC----KNVQLHFCRDSEAVVIGDLSL 116 EH DH+ GV AR++ +P++ T+QA+ E+A + + HF E DL + Sbjct: 59 EHSDHIKGVGIVARKYNLPIYANAKTWQAM-EKALGTLTTDQRFHF-EIGEVKTFADLEV 116 Query: 117 MPYTVPHDAREPVQYVAGDGKRKLGVLTDAGQSTTHLVQALGGCDALVLECNHDRQMLAD 176 + V HDA EP+ YV +KL + TD G + + + + D L+ E NHD ML Sbjct: 117 ESFGVSHDAAEPMFYVFHHDGKKLVLATDMGYVSERIKKTIENADTLIFEANHDLNMLRM 176 Query: 177 SAYPPSLKRRIGGAYGHLANDTSAEILAALDKQKLKTVIGAHLSLKNNTPGLARARARAV 236 YP ++KRRI GH++N+ +A LA + + + + AHLSL NN LAR V Sbjct: 177 GRYPWNVKRRILSDVGHVSNEDAAYALAEVIGDRTERIYLAHLSLDNNMKELARLTVEQV 236 >lin0319 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Lin Listeria innocua Length = 276 Score = 138 bits (348), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 76/235 (32%), Positives = 129/235 (54%), Gaps = 12/235 (5%) Query: 1 LKFASLGSGSEGNALIISGTSGATTTTVMLDCGFGIKETERRLTRIGKLPQDINGIVVTH 60 ++F+ L SGS GNA ++ +++DCG K+ E ++G+ D++ I++TH Sbjct: 15 IRFSILASGSSGNATLVE----TGDQKILIDCGLSGKKMEGLFAQVGRDMNDLDAILITH 70 Query: 61 EHQDHVGGVFKFARRHRIPVWLTYGTFQA----VGEEACKNVQLHFCRDSEAV-VIGDLS 115 EH DH+ G+ AR++++P++ T++A +GE + + F D E V G + Sbjct: 71 EHSDHIKGLGVLARKYKLPIYANAKTWKAMDNMIGEVSSEQ---KFQFDMETVKSFGSMQ 127 Query: 116 LMPYTVPHDAREPVQYVAGDGKRKLGVLTDAGQSTTHLVQALGGCDALVLECNHDRQMLA 175 + + V HDA EP+ Y+ G +K ++TD G + + + G DA + E NHD +ML Sbjct: 128 VESFGVSHDAIEPMFYIFHKGNKKFVMITDTGYVSDRMKGHIAGADAYLFESNHDVEMLR 187 Query: 176 DSAYPPSLKRRIGGAYGHLANDTSAEILAALDKQKLKTVIGAHLSLKNNTPGLAR 230 YP ++KRRI G GH++N+ +A ++ + + K + HLS NN LAR Sbjct: 188 MGRYPWNVKRRILGDEGHVSNEDAAIAMSEVITDQTKRIYLGHLSKDNNMKELAR 242 >CAC3538 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Cac Clostridium acetobutylicum Length = 261 Score = 137 bits (344), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 80/233 (34%), Positives = 126/233 (54%), Gaps = 11/233 (4%) Query: 1 LKFASLGSGSEGNALIISGTSGATTTTVMLDCGFGIKETERRLTRIGKLPQDINGIVVTH 60 +KF SL SGS GN++ ++ + + V++D G K E+ L I + P +I+GI VTH Sbjct: 1 MKFCSLYSGSSGNSIFVA----SDNSKVLIDAGLSGKTIEKALKEIKEDPNEIDGIFVTH 56 Query: 61 EHQDHVGGVFKFARRHRIPVWLTYGTFQA----VGEEACKNVQLHFCRDSEAVVIGDLSL 116 EH DH+ GV +R++ IP++ T++A +G+ N+++ + + I D+++ Sbjct: 57 EHSDHIKGVGVLSRKYNIPIYANELTWKAMMNSIGKIKDDNIKI---ITQDYIEIKDMNI 113 Query: 117 MPYTVPHDAREPVQYVAGDGKRKLGVLTDAGQSTTHLVQALGGCDALVLECNHDRQMLAD 176 + Y +PHDA P Y G + V TD G + + D ++LE NHD +ML Sbjct: 114 LNYKIPHDAASPCGYKILSGNKSACVATDLGHFADEVKSKISDADVILLESNHDVEMLKF 173 Query: 177 SAYPPSLKRRIGGAYGHLANDTSAEILAALDKQKLKTVIGAHLSLKNNTPGLA 229 YP LKRRI GHL+N+ + + + K+ K +I HLS NN P LA Sbjct: 174 GPYPYPLKRRILSEVGHLSNEDCGKAIVDIAKKGKKKIILGHLSRTNNYPELA 226