BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0059.p (343 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll3840 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 278 1e-74 CC3383 [R] COG1752 Predicted esterase of the alpha-beta hydrolas... 254 2e-67 RSc2551 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 242 9e-64 mlr3635 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 91 3e-18 mlr2398 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 89 1e-17 >mll3840 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Mlo Mesorhizobium loti Length = 376 Score = 278 bits (711), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 143/341 (41%), Positives = 207/341 (60%), Gaps = 14/341 (4%) Query: 2 QNKIVTLALQGGGSHGAFTWGVLDRLLEDQRIDFEGISGASAGPXAMNATVLAHGYNLGG 61 +NK + +ALQGGGSHGAF+WGVLDRLLED R++ +SG SAG AMNA LA G+ GG Sbjct: 9 ENKKINIALQGGGSHGAFSWGVLDRLLEDGRLEISAVSGTSAG--AMNAVALADGFVRGG 66 Query: 62 REGAREALAAFWGRIATKEPFSFLPPEMLPGGSSLLN---QSTPAAMHALLAMTRFFSPT 118 +GAR+ L FW +A K FS P + +P + N ++TP + M+R FSP Sbjct: 67 VDGARQKLDDFWRAVAAKGRFS--PVQRMPWDVAWGNWSIENTPGYVF-FDTMSRVFSPY 123 Query: 119 QLNPLDINPLRDILEEQVDFERLRSSGDIKLFIAATRVSTGTLKIFRNRELTVDALLASA 178 NPL +NPLRD++ +++DF+ +R+ ++LFI+AT V TG L++F + E+ +D ++ASA Sbjct: 124 VANPLGLNPLRDVVAQEIDFKNVRACKSMELFISATNVETGQLRVFSDGEIDLDTVMASA 183 Query: 179 CLPSMHRPVEIDGETYWDGGLTANPPIFPLLHLCTARDLLVVLLHPCSRPGTPTSTQEIG 238 CLP + R VEI G YWDGG NP ++P D+L+V ++P R GTP S EI Sbjct: 184 CLPQLFRAVEIKGVPYWDGGYGGNPALYPFFKTAATEDVLLVQINPVVREGTPRSANEIQ 243 Query: 239 QRLSEISFSSAFFTELGGLALAKREAENAMFAFGTLER-RLRKLNMHLVEANDLMSQLDV 297 R+ EI+F++ E +A K + A G L R + MH ++A++ L Sbjct: 244 NRIDEITFNAGLLREFRSIAFVKE-----LIAAGRLPHGEYRDIRMHRIDADEAFKDLSA 298 Query: 298 MSKLNTKTSFIHGLHDEGRQAAGRWLEKNFHMIGLKSSFDL 338 SK+N + +F+ L D GR AA WLE+N+ +G + + DL Sbjct: 299 SSKVNAEWAFLTYLRDLGRTAASDWLEENYDAVGKRPTLDL 339 >CC3383 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Ccr Caulobacter vibrioides Length = 353 Score = 254 bits (650), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 139/338 (41%), Positives = 202/338 (59%), Gaps = 10/338 (2%) Query: 6 VTLALQGGGSHGAFTWGVLDRLLEDQRIDFEGISGASAGPXAMNATVLAHGYNLGGREGA 65 ++LALQGGG+HGAF WGVLDRLLE+ ++ ++ ASAG AMNA LA G GR GA Sbjct: 16 LSLALQGGGAHGAFEWGVLDRLLEEDDLEIRAVTAASAG--AMNAVCLAQGLIDEGRAGA 73 Query: 66 REALAAFW---GRIATKEPF--SFLPPEMLPGGSSLLNQSTPAAMHALLAMTRFFSPTQL 120 R L +FW R + F + + GG L + M LA++ SP + Sbjct: 74 RARLESFWRNVNRAGGRNVFGDTGIWTNAFGGGVDWLKNTPGWRMAETLALS--LSPYEF 131 Query: 121 NPLDINPLRDILEEQVDFERLRSSGDIKLFIAATRVSTGTLKIFRNRELTVDALLASACL 180 NP ++NPL D+LE ++ F R+R + IKL+I+AT++ T K+FR ELT ++ASACL Sbjct: 132 NPFNLNPLHDVLEGEITFSRIRDASPIKLYISATQIRTSKSKVFRETELTSRHIMASACL 191 Query: 181 PSMHRPVEIDGETYWDGGLTANPPIFPLLHLCTARDLLVVLLHPCSRPGTPTSTQEIGQR 240 P + + VEIDGE YWDGG ANPP++PL + T D+L+V L+P R TP S EI R Sbjct: 192 PQLFQAVEIDGEPYWDGGYLANPPLWPLFYDDTPDDILIVTLNPFVREETPKSPGEIMDR 251 Query: 241 LSEISFSSAFFTELGGLALAKREAENAMFAFGTLERRLRKLNMHLVEANDLMSQLDVMSK 300 L+EI+F+++ +EL + ++ + + R R++ +H + A+ ++ L + +K Sbjct: 252 LNEITFNASLVSELRAIGFVQKLLDEGLLK-DNARGRYRRMLVHAITADKPLADLSMSTK 310 Query: 301 LNTKTSFIHGLHDEGRQAAGRWLEKNFHMIGLKSSFDL 338 NT+ SF+ L D GR AA WL+ +G+KSS DL Sbjct: 311 FNTEWSFLTDLKDRGRAAAQVWLDDCGDCVGVKSSVDL 348 >RSc2551 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Rso Ralstonia solanacearum Length = 349 Score = 242 bits (618), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 139/341 (40%), Positives = 199/341 (58%), Gaps = 10/341 (2%) Query: 2 QNKIVTLALQGGGSHGAFTWGVLDRLLEDQRIDFEGISGASAGPXAMNATVLAHGYNLGG 61 K + LALQGGG+HGAFTWGVLD LLED R+ FEG+SG SAG +MNA V+ G GG Sbjct: 5 HGKHINLALQGGGAHGAFTWGVLDALLEDGRLSFEGLSGTSAG--SMNAVVMLDGLLEGG 62 Query: 62 REGAREALAAFWGRIATK-EPFSFL--PPEMLPGGSSLLNQSTPAAMHALLAMTRFFSPT 118 E ARE L AFW ++T PFS + + L G S P A + + + T Sbjct: 63 PERAREKLHAFWLAVSTAGSPFSTMGENAKTLLSGRPFFPDSWPVADWMRMWDSWISATT 122 Query: 119 QLNPLDINPLRDILEEQVDFERLRSSGDIKLFIAATRVSTGTLKIFRNRELTVDALLASA 178 Q NPLRD+L QVDF+RLR+ D KLF+AAT V+TG +++F E+ V+ +LASA Sbjct: 123 Q--GAHYNPLRDLLANQVDFDRLRACPDTKLFLAATDVNTGNVRVFGTPEIDVEVVLASA 180 Query: 179 CLPSMHRPVEIDGETYWDGGLTANPPIFPLLHLCTARDLLVVLLHPCSRPGTPTSTQEIG 238 CLP ++ +EID YWDGG NP +FP + +D+L+V ++P R P E+ Sbjct: 181 CLPYLYPAIEIDRHHYWDGGYMGNPVLFPFFYETKTQDILLVHVNPIVRKDVPDQPHEVM 240 Query: 239 QRLSEISFSSAFFTELGGLALAKR-EAENAMFAFGTLERRLRKLNMHLVEANDLMSQLDV 297 +RL+EI+F+++ E+ +A ++ AE+ + RL+ + +H + A+ + L Sbjct: 241 ERLNEITFNASLLREMRAIAFVQKLIAEDWLKP--EYRDRLKYIYLHAIRADRPLGDLSS 298 Query: 298 MSKLNTKTSFIHGLHDEGRQAAGRWLEKNFHMIGLKSSFDL 338 +KL T SF+ L + GRQ A WL K+F +G + D+ Sbjct: 299 STKLVTDWSFLTMLKERGRQEAKLWLRKHFDDVGRNGTVDI 339 >mlr3635 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Mlo Mesorhizobium loti Length = 361 Score = 90.9 bits (224), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 77/259 (29%), Positives = 119/259 (45%), Gaps = 31/259 (11%) Query: 8 LALQGGGSHGAFTWGVLDRLLEDQRIDFEGISGASAGPXAMNATVLAHGYNLGGREGAR- 66 + QGGG+ GA+ GV + L D + +SG S G A+NA+++A G E +R Sbjct: 1 MVFQGGGALGAYQAGVY-QALADAGCEPSWLSGVSIG--AINASIIA------GNEPSRR 51 Query: 67 -EALAAFWGRIATKEPFSFLPPEMLPGGSSLLNQSTPAAMHALLAMTRFFSPTQLNP--- 122 + L FW I+ ++ +S+ P + + T + M + FF P NP Sbjct: 52 LQRLEQFWQTISGRKIWSYTPEGDI---YRDIRNRTSSWMTMTMGQPGFFKPRNPNPWFE 108 Query: 123 ----------LDINPLRDILEEQVDFERLRSSGDIKLFIAATRVSTGTLKIFRNRELTV- 171 D L+D LE +DF+ L + G +L + A V TG F ++ + Sbjct: 109 PQGAEGATSFYDTAELKDTLESLIDFDVL-NDGKKRLSVGAVNVRTGNFVYFDTEKVRIG 167 Query: 172 -DALLASACLPSMHRPVEIDGETYWDGGLTANPPIFPLLHLCTARDLLVVLLHPCSRPGT 230 + ++AS LP V I+GE YWDGG+ +N P+ LL R LV + S G Sbjct: 168 PEHIMASGALPPAFPSVRIEGEYYWDGGIVSNTPLQYLLDQEEDRSSLVFQVDLFSARGV 227 Query: 231 -PTSTQEIGQRLSEISFSS 248 P S ++ R +I +SS Sbjct: 228 LPRSMPDVLSRHKDIMYSS 246 >mlr2398 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Mlo Mesorhizobium loti Length = 390 Score = 89.4 bits (220), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 80/267 (29%), Positives = 123/267 (46%), Gaps = 32/267 (11%) Query: 4 KIVTLALQGGGSHGAFTWGVLDRLLEDQRIDFEGISGASAGPXAMNATVLAHGYNLGGRE 63 + + L LQGGG+ GA+ GV + L E I + ++G S G A+N+ ++A G E Sbjct: 19 ECIALVLQGGGALGAYQAGVYEALAEAD-IHPDWVAGVSIG--AINSAIIA------GNE 69 Query: 64 GAREA--LAAFWGRIATKE--PFSFLPPEMLPGGS--SLLNQSTPAAMHALLAMTRFFSP 117 L AFW I+ ++ P + P G+ LL AA + FFS Sbjct: 70 PTERVPKLRAFWREISANPLLDWAAASPALAPTGTLPRLLFNQFSAACALVAGAPGFFSL 129 Query: 118 TQLNP-------------LDINPLRDILEEQVDFERLRSSGDIKLFIAATRVSTGTLKIF 164 Q P + L+ LE VDF+R+ ++G ++ + A V +G F Sbjct: 130 RQPVPWLQADGSLEGTSFYETKHLKSTLERLVDFDRI-NAGRMRFSVGAVNVRSGNFVYF 188 Query: 165 RNRELTV--DALLASACLPSMHRPVEIDGETYWDGGLTANPPIFPLLHLCTARDLLVVLL 222 N + T+ + ++AS LP VEIDGE YWDGGL +N P+ ++ +D L + Sbjct: 189 DNDQHTIRPEHVMASGSLPPGFPAVEIDGEFYWDGGLISNTPLQWVVEHGARQDTLAFQV 248 Query: 223 HPCSRPGT-PTSTQEIGQRLSEISFSS 248 S G P + E+ R EI ++S Sbjct: 249 DLWSARGEFPRNLSEVATRQKEIQYAS 275