BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0023.p (295 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2025 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 315 6e-86 BMEI0972 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase compl... 273 4e-73 AGc2967 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 266 5e-71 all4968 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 263 3e-70 CC2302 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 263 5e-70 >PA2025 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Pae Pseudomonas aeruginosa Length = 451 Score = 315 bits (808), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 165/294 (56%), Positives = 209/294 (71%), Gaps = 2/294 (0%) Query: 2 SDDFFHLTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAE 61 S++ F L LP+R +V+GGGYIAVE ASI NGLG+E TL+YR LRG D + L + Sbjct: 156 SNEAFFLERLPRRVLVVGGGYIAVEFASIFNGLGAETTLLYRRDLFLRGFDRSVREHLRD 215 Query: 62 EMRKKGMNILFENNIDAIASDGAGK-RVKLSDGKTVDADCVLFATGRHPNTAGLGLEKVG 120 E+ KKG+++ F ++I I G L DG+ ++ADCV +ATGR P LGLE Sbjct: 216 ELGKKGLDLQFNSDIARIDKQADGSLAATLKDGRVLEADCVFYATGRRPMLDDLGLENTA 275 Query: 121 VALTAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARLFN-QGGREMSY 179 V LT KG + VD+H+ + SI A+GDVI RV LTPVALAEGM VA RLF + R + Y Sbjct: 276 VKLTDKGFIAVDEHYQTSEPSILALGDVIGRVQLTPVALAEGMAVARRLFKPEEYRPVDY 335 Query: 180 ENIPTAVFSHPNVGTVGLGEEDARKKVGEVRIFKSEFKALKHTLSGNAERTFMKLVVDAK 239 + IPTAVFS PN+GTVGL EE+A +V+IF+S F+ +K TL+ + E+T MKLVVDA Sbjct: 336 KLIPTAVFSLPNIGTVGLTEEEALSAGHKVKIFESRFRPMKLTLTDDQEKTLMKLVVDAH 395 Query: 240 NDRVLGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAEEFVTMRTP 293 +DRVLG HMVGA+AGEI+QG AVA++ GATK FD TIGIHPT+AEEFVT+RTP Sbjct: 396 DDRVLGCHMVGAEAGEILQGIAVAMKAGATKQAFDETIGIHPTAAEEFVTLRTP 449 >BMEI0972 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Bme Brucella melitensis Length = 483 Score = 273 bits (698), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 140/294 (47%), Positives = 194/294 (65%), Gaps = 1/294 (0%) Query: 2 SDDFFHLTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAE 61 S++ FHL LPK V+ GGGYIAVE A+I +GLG E TLVYRGK +L D ++ L E Sbjct: 179 SNEAFHLEELPKSIVIAGGGYIAVEFANIFHGLGVETTLVYRGKEILSRFDHDIRSLLHE 238 Query: 62 EMRKKGMNILFENNIDAIASDGAGK-RVKLSDGKTVDADCVLFATGRHPNTAGLGLEKVG 120 M KG+ IL + + GK V+LS+G+T++ D + A GR PNT GLGLEK G Sbjct: 239 TMEAKGIRILCGAIFEKVEKKADGKLSVELSNGETLETDQAMLALGRIPNTDGLGLEKAG 298 Query: 121 VALTAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARLFNQGGREMSYE 180 V + GA++VD + ++V +I A+GDV +RV LTPVA+ E M F + +E Sbjct: 299 VKIDKSGAIEVDDYSRTNVENIWAVGDVTNRVQLTPVAIHEAMCFLETAFKDNPTKPDHE 358 Query: 181 NIPTAVFSHPNVGTVGLGEEDARKKVGEVRIFKSEFKALKHTLSGNAERTFMKLVVDAKN 240 I TAVFS P +GTVGL E++A KK E+ I+++ F+ +K+TLSG +E+T MKLVV++K+ Sbjct: 359 LIATAVFSQPEIGTVGLSEDEAAKKYPELEIYRALFRPMKNTLSGRSEKTLMKLVVESKS 418 Query: 241 DRVLGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAEEFVTMRTPV 294 +VLG H++G DAGE+ Q ++L+ GATK FD T+ +HPT+ EE VTM P Sbjct: 419 RKVLGAHIMGPDAGEMAQLLGISLKAGATKDDFDRTMAVHPTATEELVTMYKPT 472 >AGc2967 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 464 Score = 266 bits (679), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 136/293 (46%), Positives = 191/293 (65%), Gaps = 1/293 (0%) Query: 2 SDDFFHLTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAE 61 S++ F L PK ++ GGGYIAVE A+I +GLG E TL+YRGK +L D ++ L E Sbjct: 162 SNEAFDLEEPPKSILIAGGGYIAVEFANIFHGLGVETTLIYRGKEILSRFDHDMRKGLHE 221 Query: 62 EMRKKGMNILFENNIDAIASDGAGKRV-KLSDGKTVDADCVLFATGRHPNTAGLGLEKVG 120 M KG+ I+ E+ I+ ++ DGAG V + G ++ V+ A GR PNT GLGLE G Sbjct: 222 AMEAKGIRIILEDIIEEVSKDGAGGFVARTKQGSSLHVGLVMLALGRDPNTKGLGLENAG 281 Query: 121 VALTAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARLFNQGGREMSYE 180 V + A+GA+ VD + ++V SI A+GDV DRV LTPVA+ E M + + +E Sbjct: 282 VKVDARGAIIVDDYSRTNVPSIFALGDVTDRVQLTPVAIHEAMCFIETEYKNNPTKPDHE 341 Query: 181 NIPTAVFSHPNVGTVGLGEEDARKKVGEVRIFKSEFKALKHTLSGNAERTFMKLVVDAKN 240 I TAVFS P +GTVGL EE+A KK E+ +++++F+ +K TLSG E+T MKL+V+A + Sbjct: 342 LIATAVFSQPEIGTVGLSEEEAGKKYRELEVYRAQFRPMKATLSGRQEKTIMKLIVNASD 401 Query: 241 DRVLGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAEEFVTMRTP 293 RV+GVH++G +AGE+ Q + L+ G TK FD T+ +HPT+AEE VTM P Sbjct: 402 RRVVGVHILGHEAGEMAQLLGITLKAGCTKDDFDRTMAVHPTAAEELVTMYAP 454 >all4968 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Nos Nostoc sp. PCC 7120 Length = 459 Score = 263 bits (672), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 139/304 (45%), Positives = 190/304 (62%), Gaps = 14/304 (4%) Query: 2 SDDFFHLTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAE 61 S++ FHL PK ++G GYI E A I+ GLGS+VT + RG +L+G D ++ + E Sbjct: 156 SNEIFHLKTQPKHIAIIGSGYIGTEFAGIMRGLGSQVTQITRGDKILKGFDEDIRTEIQE 215 Query: 62 EMRKKGMNILFENNIDAIASDGAGKRVKLS--DGKTVDADCVLFATGRHPNTAGLGLEKV 119 M G+ I+ +N + AI G ++ LS D + + AD L ATGR PN GLGLE Sbjct: 216 GMTNHGIRIIPKNVVTAIEQVPEGLKISLSGEDQEPIIADVFLVATGRVPNVDGLGLENA 275 Query: 120 GV----------ALTAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARL 169 GV + A+ V+++ + +I+A+GDV DR+ LTPVA+ EG A Sbjct: 276 GVDVVDSSIEGPGYSTMNAIAVNEYSQTSQPNIYAVGDVTDRLNLTPVAIGEGRAFADSE 335 Query: 170 FNQGGREMSYENIPTAVFSHPNVGTVGLGEEDARKKVGE--VRIFKSEFKALKHTLSGNA 227 F RE S+E I TAVFS+P TVGL E +AR K+G+ V I+++ F+ + H+ +G Sbjct: 336 FGNNRREFSHETIATAVFSNPQASTVGLTEAEARAKLGDDAVTIYRTRFRPMYHSFTGKQ 395 Query: 228 ERTFMKLVVDAKNDRVLGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAEEF 287 ER MKLVVD K D+VLG HMVG +A EI+QG A+A++ GATK FDAT+GIHP+SAEEF Sbjct: 396 ERIMMKLVVDTKTDKVLGAHMVGENAAEIIQGVAIAVKMGATKKDFDATVGIHPSSAEEF 455 Query: 288 VTMR 291 VTMR Sbjct: 456 VTMR 459 >CC2302 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Ccr Caulobacter vibrioides Length = 466 Score = 263 bits (671), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 135/291 (46%), Positives = 192/291 (65%), Gaps = 1/291 (0%) Query: 2 SDDFFHLTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAE 61 SD+ FHL LPK +V+GGGYIAVE A I GLG + TL+YRG ++LRG D ++ + LA+ Sbjct: 164 SDEAFHLPTLPKSVLVVGGGYIAVEFAGIYAGLGVQTTLLYRGANILRGFDDDVRMHLAD 223 Query: 62 EMRKKGMNILFENNIDAIASDGAGKRVK-LSDGKTVDADCVLFATGRHPNTAGLGLEKVG 120 E+ K+G+ ++ + +I G+ + LS+ T + + V+FATGR P GLGLEK G Sbjct: 224 ELEKRGIKVVLGCSHKSIEKLDDGRLLSTLSNDLTFETEAVMFATGREPYVQGLGLEKAG 283 Query: 121 VALTAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARLFNQGGREMSYE 180 V L KGA+ VD++ ++V SI A+GDV DR+ LTPVA+ EG A F ++ Sbjct: 284 VKLNDKGAIAVDKYSKTNVDSIWAVGDVTDRINLTPVAIREGAAFAQTEFYGNPTTFDHD 343 Query: 181 NIPTAVFSHPNVGTVGLGEEDARKKVGEVRIFKSEFKALKHTLSGNAERTFMKLVVDAKN 240 + +AVFS P VG VG+ E +AR+ G+V I++S F+ +K T G ER +KLVV + Sbjct: 344 LVASAVFSQPPVGAVGMSEAEARQAFGKVDIYRSIFRPMKVTFYGGQERCLIKLVVKQDD 403 Query: 241 DRVLGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAEEFVTMR 291 +R+LGVH+VG D+ EI+Q A+A++ G TK Q+D+T +HPT AEE VTMR Sbjct: 404 ERILGVHVVGPDSPEIIQMAAIAVKMGVTKPQWDSTCAVHPTLAEELVTMR 454