BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0010.p (294 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc00265 [G] COG1638 TRAP-type C4-dicarboxylate transport system... 205 1e-52 AGl115 [G] COG1638 TRAP-type C4-dicarboxylate transport system, ... 197 2e-50 HI1028 [G] COG1638 TRAP-type C4-dicarboxylate transport system, ... 87 3e-17 PM1252 [G] COG1638 TRAP-type C4-dicarboxylate transport system, ... 86 9e-17 yiaO [G] COG1638 TRAP-type C4-dicarboxylate transport system, pe... 85 2e-16 >SMc00265 [G] COG1638 TRAP-type C4-dicarboxylate transport system, periplasmic component Sme Sinorhizobium meliloti Length = 336 Score = 205 bits (521), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 118/253 (46%), Positives = 154/253 (60%), Gaps = 13/253 (5%) Query: 8 ITRRRFLKTVSVASAAAATGM-ISTQAFAAAEFTLKYANNLPATHPMNLRAKEMAAKIAT 66 +TRR L + + A A G I T+A AA +FT K ANNLP THP+N+R KE A KI Sbjct: 3 MTRRTML----LGTGAVALGASIRTKAHAA-DFTYKLANNLPVTHPLNIRLKEAADKILE 57 Query: 67 ESKGRIDFQVYPNNQLGNDTDTLGQVRSGAVDFFTLSPLILGTLVPAAQISGVGFAFKDY 126 E+ GR+ ++P++QLGNDT+TL Q+R+GA +FF+LSPLIL TLVP A ISG+GFAF DY Sbjct: 58 ETGGRLKINIFPSSQLGNDTETLSQLRNGATEFFSLSPLILSTLVPNAAISGIGFAFPDY 117 Query: 127 NQVWAALDGELGAHVRKQIDAKSTVFAFEKIWDNGYRQITNSTRAINKPEDLKGMKLRXI 186 + VW A+DGELGA+ R +I+ K V EKIWDNG+RQIT+S + IN PEDLKG K+R Sbjct: 118 DTVWKAMDGELGAYARGEIEKKGLV-PMEKIWDNGFRQITSSNKPINTPEDLKGFKIRVP 176 Query: 187 NKP--EDLKGMKSPAPSAIAFPPVRHR----SAAPRPRHIDVGSAGTSAYRQPRCAHTNP 240 P + + AP+ I F V + + + + S Q CA TN Sbjct: 177 PSPLWQSMFTAFGAAPTTINFAEVYTALSTGTVDGQENPLAIASTAKLYEVQKHCAMTNH 236 Query: 241 FADRSRAIAGNSA 253 D +A A Sbjct: 237 MWDGFWMLANKQA 249 >AGl115 [G] COG1638 TRAP-type C4-dicarboxylate transport system, periplasmic component Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 347 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 105/205 (51%), Positives = 138/205 (67%), Gaps = 9/205 (4%) Query: 7 SITRRRFLKTVSVASAAAATGMISTQAFAAAEFTLKYANNLPATHPMNLRAKEMAAKIAT 66 +I RR L SA AT +S AA++ KYANNLPA HPMN+RAKE A +IA Sbjct: 13 NINRRSIL--AGAGSAVLATAFVSRAH--AAQYNYKYANNLPAAHPMNIRAKEAAERIAK 68 Query: 67 ESKGRIDFQVYPNNQLGNDTDTLGQVRSGAVDFFTLSPLILGTLVPAAQISGVGFAFKDY 126 E+ G+++ ++P++QLG DTD L QVRSG V+FFTLSPLIL TL+P A ISG+GFAF +Y Sbjct: 69 ETDGQVNISIFPSSQLGADTDVLSQVRSGGVEFFTLSPLILSTLIPNASISGIGFAFPNY 128 Query: 127 NQVWAALDGELGAHVRKQIDAKSTVFAFEKIWDNGYRQITNSTRAINKPEDLKGMKLRXI 186 VWAA+DG+LG +VR +I AK + A + IWDNG+RQIT++++ I P DL+ K+R Sbjct: 129 ESVWAAMDGDLGKYVRGEI-AKQNLVAMDTIWDNGFRQITSASKPIATPADLESFKIRVP 187 Query: 187 NKP---EDLKGMKSPAPSAIAFPPV 208 P K KS AP++I F V Sbjct: 188 VSPLWTSMFKAFKS-APASINFAEV 211 >HI1028 [G] COG1638 TRAP-type C4-dicarboxylate transport system, periplasmic component Hin Haemophilus influenzae Length = 328 Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 54/176 (30%), Positives = 91/176 (51%), Gaps = 10/176 (5%) Query: 35 AAAEFTLKYANNLPATHPMNLRAKEMAAKIATESKGRIDFQVYPNNQLGNDTDTLGQVRS 94 A AE +L++ P + + AK+ + ++KG I +++P++ LGN + VR Sbjct: 23 ALAEISLRFGYEAPRSDSQHSAAKKFNDLLMKKTKGEIKLKLFPDSTLGNAQTMISSVRG 82 Query: 95 GAVDF-FTLSPLILGTLVPAAQISGVGFAFKDYNQVWAALDGELGAHVRKQIDAKSTV-F 152 G +D + SP G L P + + F FKD V+ LDGE+G ++ K ++ + Sbjct: 83 GTIDLEMSGSPNFTG-LEPKLNVIDIPFIFKDREHVYKVLDGEVGQNLLKDLEKQGLKGL 141 Query: 153 AFEKIWDNGYRQITNSTRAINKPEDLKGMKLRXINKPEDLKGMK----SPAPSAIA 204 AF WD G+R +NS + + KPE +KG+K+R P ++ K +P P +A Sbjct: 142 AF---WDVGFRAFSNSKQTVTKPEHIKGLKVRTNQNPMYIEAFKLLGSNPVPMPLA 194 >PM1252 [G] COG1638 TRAP-type C4-dicarboxylate transport system, periplasmic component Pmu Pasteurella multocida Length = 328 Score = 85.9 bits (211), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 54/170 (31%), Positives = 87/170 (51%), Gaps = 6/170 (3%) Query: 22 AAAATGMISTQAFAAAEFTLKYANNLPATHPMNLRAKEMAAKIATESKGRIDFQVYPNNQ 81 AA GM A A A TL++ P + + AK+ + ++KG I ++P++ Sbjct: 10 AALVAGMAVFTASANAATTLRFGYEAPRSDTQHEAAKKFNELLKEKTKGEIKLSLFPDST 69 Query: 82 LGNDTDTLGQVRSGAVDF-FTLSPLILGTLVPAAQISGVGFAFKDYNQVWAALDGELGAH 140 LGN + VR G +D + SP G LVP + + F F++ +A LDGE+G Sbjct: 70 LGNAQTMISAVRGGTIDLEMSGSPNFSG-LVPKLNVIDIPFIFQNREHAYAVLDGEIGQG 128 Query: 141 VRKQIDAKSTV-FAFEKIWDNGYRQITNSTRAINKPEDLKGMKLRXINKP 189 + K+++A+ AF W+ G+R TNS + P+D+KG+K+R P Sbjct: 129 LLKELEAQGLKGLAF---WEVGFRSFTNSKHPVKTPDDIKGLKVRTNQNP 175 >yiaO [G] COG1638 TRAP-type C4-dicarboxylate transport system, periplasmic component Eco Escherichia coli K12 Length = 328 Score = 85.1 bits (209), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 58/216 (26%), Positives = 100/216 (46%), Gaps = 12/216 (5%) Query: 14 LKTVSVASAAAATGMISTQAFAAAEFTLKYANNLPATHPMNLRAKEMAAKIATESKGRID 73 L++V+ A A ST + AA +L++ T ++ AK+ + +KG + Sbjct: 3 LRSVTYALFIAGLAAFSTSSLAAQ--SLRFGYETSQTDSQHIAAKKFNDLLQERTKGELK 60 Query: 74 FQVYPNNQLGNDTDTLGQVRSGAVDFFTLSPLILGTLVPAAQISGVGFAFKDYNQVWAAL 133 +++P++ LGN + VR G +D L P + V F F+D L Sbjct: 61 LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTL 120 Query: 134 DGELGAHVRKQIDAKST-VFAFEKIWDNGYRQITNSTRAINKPEDLKGMKLRXINKPEDL 192 DG++G ++ ++ K V A+ W+NG+R +TNS + P DLKG+K+R N P ++ Sbjct: 121 DGKVGDDLKASLEGKGLKVLAY---WENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNI 177 Query: 193 KGMKS------PAPSAIAFPPVRHRSAAPRPRHIDV 222 K P P A + + R+ + I+V Sbjct: 178 AAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINV 213