BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0081.p (244 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1390 [K] COG1191 DNA-directed RNA polymerase specialized sigm... 294 1e-79 fliA [K] COG1191 DNA-directed RNA polymerase specialized sigma s... 227 2e-59 ZfliA [K] COG1191 DNA-directed RNA polymerase specialized sigma ... 227 2e-59 ECs2661 [K] COG1191 DNA-directed RNA polymerase specialized sigm... 227 2e-59 STM1956 [K] COG1191 DNA-directed RNA polymerase specialized sigm... 226 3e-59 >RSp1390 [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Rso Ralstonia solanacearum Length = 253 Score = 294 bits (753), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 141/240 (58%), Positives = 181/240 (75%), Gaps = 2/240 (0%) Query: 1 MYNVKGKTAKNDLLQLHAALVKKLAHQMKAKLPPSVEVDDLIQAGMIGLLDAVNRYEETH 60 MY G+ K+D + +A LV+++A+QM KLP SVE+DDLIQAGM+GLLDA++RYEET Sbjct: 11 MYTATGRKTKSDEMAAYAPLVRRIANQMMVKLPASVELDDLIQAGMMGLLDALSRYEETQ 70 Query: 61 GAQFETYAVQRIRGAMLDELRSSDWLPRSIRQNTRKIEEAIHVLLQQLGRPPQEAEVATQ 120 G QFE YA QRIRGA+LDELR SDWLPR +R+N R IE+AI L QQ GR P E E+A Sbjct: 71 GVQFEFYAAQRIRGAILDELRGSDWLPRGLRRNARTIEQAIQKLQQQKGRAPTETEIAAA 130 Query: 121 LKLSLPEYQDMLTECGGHQLIYYEDFHDADGGHEHFLDRYQTRDTDDPLQNLINGGFRDA 180 L +SL +YQ +L E G QL+YYEDF D + F++ + PL L++ R++ Sbjct: 131 LNMSLADYQKLLVELQGSQLLYYEDFDREDA--DAFIENRAPENDATPLDQLLDHDLRES 188 Query: 181 VIDSIKALPEREKILMGLYYEQEMNLKEIGAVMGVSESRVCQLHSQAIARMRSTLREQAW 240 V+ +I+ALPEREK++M LYYEQ++NL+EIGAVMGVSESRVCQ+HSQAIAR+RS LRE+AW Sbjct: 189 VVAAIEALPEREKLMMSLYYEQDLNLREIGAVMGVSESRVCQIHSQAIARIRSRLRERAW 248 >fliA [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Eco Escherichia coli K12 Length = 239 Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 116/236 (49%), Positives = 164/236 (69%), Gaps = 4/236 (1%) Query: 1 MYNVKGKTAKNDLLQLHAALVKKLAHQMKAKLPPSVEVDDLIQAGMIGLLDAVNRYEETH 60 +Y +G K+ L Q + LV+ A +++ +LP SVE+DDL+QAG IGLL+AV RY+ Sbjct: 4 LYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVERYDALQ 63 Query: 61 GAQFETYAVQRIRGAMLDELRSSDWLPRSIRQNTRKIEEAIHVLLQQLGRPPQEAEVATQ 120 G F TYAVQRIRGAMLDELRS DW+PRS+R+N R++ +AI L Q+LGR E EVA + Sbjct: 64 GTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQAIGQLEQELGRNATETEVAER 123 Query: 121 LKLSLPEYQDMLTECGGHQLIYYEDFHDADGGH-EHFLDRYQTRDTDDPLQNLINGGFRD 179 L + + +Y+ ML + QL Y+++ + G E D +Q ++PLQ L++ R Sbjct: 124 LGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELVTDDHQ---RENPLQQLLDSNLRQ 180 Query: 180 AVIDSIKALPEREKILMGLYYEQEMNLKEIGAVMGVSESRVCQLHSQAIARMRSTL 235 V+++I+ LPEREK+++ LYY++E+NLKEIGAV+ V ESRV QLHSQAI R+R+ L Sbjct: 181 RVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKL 236 >ZfliA [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit EcZ Escherichia coli O157:H7 EDL933 Length = 239 Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 116/236 (49%), Positives = 164/236 (69%), Gaps = 4/236 (1%) Query: 1 MYNVKGKTAKNDLLQLHAALVKKLAHQMKAKLPPSVEVDDLIQAGMIGLLDAVNRYEETH 60 +Y +G K+ L Q + LV+ A +++ +LP SVE+DDL+QAG IGLL+AV RY+ Sbjct: 4 LYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVERYDALQ 63 Query: 61 GAQFETYAVQRIRGAMLDELRSSDWLPRSIRQNTRKIEEAIHVLLQQLGRPPQEAEVATQ 120 G F TYAVQRIRGAMLDELRS DW+PRS+R+N R++ +AI L Q+LGR E EVA + Sbjct: 64 GTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQAIGQLEQELGRNATETEVAER 123 Query: 121 LKLSLPEYQDMLTECGGHQLIYYEDFHDADGGH-EHFLDRYQTRDTDDPLQNLINGGFRD 179 L + + +Y+ ML + QL Y+++ + G E D +Q ++PLQ L++ R Sbjct: 124 LGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELVTDDHQ---RENPLQQLLDSNLRQ 180 Query: 180 AVIDSIKALPEREKILMGLYYEQEMNLKEIGAVMGVSESRVCQLHSQAIARMRSTL 235 V+++I+ LPEREK+++ LYY++E+NLKEIGAV+ V ESRV QLHSQAI R+R+ L Sbjct: 181 RVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKL 236 >ECs2661 [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Ecs Escherichia coli O157:H7 Length = 239 Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 116/236 (49%), Positives = 164/236 (69%), Gaps = 4/236 (1%) Query: 1 MYNVKGKTAKNDLLQLHAALVKKLAHQMKAKLPPSVEVDDLIQAGMIGLLDAVNRYEETH 60 +Y +G K+ L Q + LV+ A +++ +LP SVE+DDL+QAG IGLL+AV RY+ Sbjct: 4 LYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVERYDALQ 63 Query: 61 GAQFETYAVQRIRGAMLDELRSSDWLPRSIRQNTRKIEEAIHVLLQQLGRPPQEAEVATQ 120 G F TYAVQRIRGAMLDELRS DW+PRS+R+N R++ +AI L Q+LGR E EVA + Sbjct: 64 GTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQAIGQLEQELGRNATETEVAER 123 Query: 121 LKLSLPEYQDMLTECGGHQLIYYEDFHDADGGH-EHFLDRYQTRDTDDPLQNLINGGFRD 179 L + + +Y+ ML + QL Y+++ + G E D +Q ++PLQ L++ R Sbjct: 124 LGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELVTDDHQ---RENPLQQLLDSNLRQ 180 Query: 180 AVIDSIKALPEREKILMGLYYEQEMNLKEIGAVMGVSESRVCQLHSQAIARMRSTL 235 V+++I+ LPEREK+++ LYY++E+NLKEIGAV+ V ESRV QLHSQAI R+R+ L Sbjct: 181 RVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKL 236 >STM1956 [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit Sty Salmonella typhimurium LT2 Length = 239 Score = 226 bits (576), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 113/235 (48%), Positives = 163/235 (69%), Gaps = 2/235 (0%) Query: 1 MYNVKGKTAKNDLLQLHAALVKKLAHQMKAKLPPSVEVDDLIQAGMIGLLDAVNRYEETH 60 +Y +G K+ L Q + LV+ A +++ +LP SVE+DDL+QAG IGLL+AV+RY+ Sbjct: 4 LYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVDRYDALQ 63 Query: 61 GAQFETYAVQRIRGAMLDELRSSDWLPRSIRQNTRKIEEAIHVLLQQLGRPPQEAEVATQ 120 G F TYAVQRIRGAMLDELRS DW+PRS+R+N R++ +A+ L Q+LGR E EVA + Sbjct: 64 GTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQAMGQLEQELGRNATETEVAER 123 Query: 121 LKLSLPEYQDMLTECGGHQLIYYEDFHDADGGHEHFLDRYQTRDTDDPLQNLINGGFRDA 180 L + + EY+ ML + QL Y+++ + G + + ++PL L+ G R Sbjct: 124 LGIPVAEYRQMLLDTNNSQLFSYDEWREEHGDSIELVT--EEHQQENPLHQLLEGDLRQR 181 Query: 181 VIDSIKALPEREKILMGLYYEQEMNLKEIGAVMGVSESRVCQLHSQAIARMRSTL 235 V+D+I++LPERE++++ LYY++E+NLKEIGAV+ V ESRV QLHSQAI R+R+ L Sbjct: 182 VMDAIESLPEREQLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKL 236