BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0064.p (627 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1086 [N] COG1256 Flagellar hook-associated protein Pae Pseudom... 236 9e-62 RSp0351 [N] COG1256 Flagellar hook-associated protein Rso Ralsto... 216 1e-55 ZflgK [N] COG1256 Flagellar hook-associated protein EcZ Escheric... 201 6e-51 ECs1460 [N] COG1256 Flagellar hook-associated protein Ecs Escher... 201 6e-51 flgK [N] COG1256 Flagellar hook-associated protein Eco Escherich... 200 8e-51 >PA1086 [N] COG1256 Flagellar hook-associated protein Pae Pseudomonas aeruginosa Length = 683 Score = 236 bits (603), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 201/648 (31%), Positives = 305/648 (47%), Gaps = 126/648 (19%) Query: 1 VLSAGSTLAARFQSLNDQMAQSRTSVNAQITSSIGSINSLATQIQELNQAIIKAQGSGGG 60 VL+ L+ F +L DQ+ + + +N Q+ + +N+L+ + E N AI KA+ +G Sbjct: 133 VLAQAQGLSKTFNTLYDQLDKQNSLINQQLGALTSQVNNLSQSVAEYNDAIAKAKSAG-- 190 Query: 61 AAPNDLLDQRDQAIADLNKIVKVTTLPQNNGSMSVFIGNGQPLVLGDQVSQLVATPADDD 120 A PNDLLD RD+A+ L+++V VT + Q++ S+S+FIG+GQPLV+G+ VS L P DD Sbjct: 191 AVPNDLLDARDEAVRKLSEMVGVTAVTQDDNSVSLFIGSGQPLVVGNTVSTLSVVPGLDD 250 Query: 121 PTRLQVSI---KTSSNATILLPDGAFTGGSLGGILQYRTETLDTAQNTLGRVAIAMASAF 177 PTR QV + ++ N T L+ +GG +GG+L YR LD++ N LG++A+ A Sbjct: 251 PTRYQVQLTLGDSTQNVTRLV-----SGGQMGGLLAYRDTVLDSSYNKLGQLALTFADTV 305 Query: 178 NAQHKLGQDQNGALGTDFF---NVPVPSVVANIKNHVALPVATATATISNPSALTTSDYR 234 N Q G D G G + F N P + + + + A I++ S L +SD+R Sbjct: 306 NKQLGQGLDLAGKAGANLFGDINDPDITALRVLAKNGNTGNVHANLNITDTSKLNSSDFR 365 Query: 235 LDYDGFNYSVTRLSDNTK---TTSATMPVTI-----DGVDFGSAGMV-----AKDSFLIK 281 LD+DG N++ RL D+ T S T P T+ +GVD G + + A D F ++ Sbjct: 366 LDFDGTNFTARRLGDDASMQVTVSGTGPYTLSFKDANGVDQGFSVTLDQLPAAGDRFTLQ 425 Query: 282 PTIDGANGISVAITNTQKIA-AGTPIVTSSNAVTNMGLVTSGNTGTGVVTSATVDKAAFA 340 PT GA+ I + N ++A AG+ + A TN N G+G + Sbjct: 426 PTRRGASDIETTLKNASQLAFAGS---ARAEATTN-------NRGSGAI----------- 464 Query: 341 PNSTQTYTYSSGTNTLALSPNPVGNVMVTTTAGVTTPYTPGTAIPYSAGATITADGVSFV 400 G L P+P+ ++ G +P S AT++ADG ++ Sbjct: 465 -----------GQPNLVDGPSPIDPAVLQNAFGA-------NGLPLS--ATVSADGKTYT 504 Query: 401 LSGTPANNDKFVFSPTPANTGTATISSGTINSSFTATTLPAGSKLSFTFDSTTGTLATSP 460 ++ +V A G+ T++SGT NS A T P GS ++T++ Sbjct: 505 MTSPLPAGWSYVDKDGNALPGSPTLNSGTSNSVRMAYTDP-GSGQTYTYE---------- 553 Query: 461 ALGTAPMGTVTVKDAGGNPVLVTPPNLPFGPGYSYTTSTGMSFSITGQPANGDQFYVSPN 520 F+++ P GD F +S N Sbjct: 554 ------------------------------------------FNLSNVPQTGDSFTLSFN 571 Query: 521 TGGVTDNRNALALNALQTANTIGNTSFQGS-----YSQLVSLIGNKTNEITVTNKAENAR 575 G+ DNRNAL LNALQT T+G T GS Y LV +G T + + A Sbjct: 572 KDGIADNRNALNLNALQTKPTVGGTDSTGSTYNDAYGGLVERVGTLTAQARASADASQTV 631 Query: 576 LTAIQQEQQSESGVNQDEELANMIKNQTAYQAAAKIIQAASDMLNVLF 623 L Q + S SGV+ DEE AN+I+ Q Y A+A++IQ A + + L Sbjct: 632 LKQAQDSRDSLSGVSLDEEAANLIQFQQYYSASAQVIQVARSLFDTLI 679 >RSp0351 [N] COG1256 Flagellar hook-associated protein Rso Ralstonia solanacearum Length = 642 Score = 216 bits (550), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 168/423 (39%), Positives = 240/423 (56%), Gaps = 25/423 (5%) Query: 2 LSAGSTLAARFQSLNDQMAQSRTSVNAQITSSIGSINSLATQIQELNQAIIKAQGSGGGA 61 LSA STL RF +++ QMA VN Q+ + + S+NS QI LN I KA+ + GG Sbjct: 135 LSAASTLQTRFNAISGQMASLSNQVNTQVQTQVNSVNSTTQQIAALNDQIAKAEAASGGQ 194 Query: 62 APNDLLDQRDQAIADLNKIVKVTTLPQNNGSMSVFIGNGQPLVLGDQVSQLVATPADDDP 121 NDL DQRDQ + LN+ +K + + ++G ++++GNGQ LV G+Q QL + DP Sbjct: 195 PANDLRDQRDQLVQTLNQSIKASVVQTSDGQYNIYVGNGQALVQGNQSYQLTTVASQYDP 254 Query: 122 TRLQVSIKTSSNATILLPDGAFTGGSLGGILQYRTETLDTAQNTLGRVAIAMASAFNAQH 181 T+L V K+ + T+ + D GG+LGG++Q+R TL AQN+LGR+A A+++ N Q+ Sbjct: 255 TQLSVGYKSPA-GTVTIDDSQLGGGALGGLMQFRQNTLIPAQNSLGRLAAAVSADVNTQN 313 Query: 182 KLGQDQNGALGTDFFNVPVPSVVANIKNHVALPVATATATISNPSALTTSDYRLDYDGFN 241 K G D NG LGTD F+ PSV A+ N TATI+N +A DY++ Y G Sbjct: 314 KEGMDLNGKLGTDLFSTAGPSVAASSSN---TGTGALTATITNANAGQGYDYQVKYSGGA 370 Query: 242 YSVTRLSDNT-KTTSATMPVTIDGVDFGSAG-MVAKDSFLIKPTIDGANGISVAITNTQK 299 Y+V+ D + T ++ P T+DGV G M + DSFL++PT++ A+ + ++ Sbjct: 371 YTVSHYPDGSGAVTVSSWPTTVDGVTLNLTGSMASGDSFLVRPTVNAASTMQTLTSDYHA 430 Query: 300 IAAGTPIVTSSNAVTNMGLVTSGNTGTGVVTSATVDKA-AFAP-NSTQTYTYSSGTNTLA 357 +A +S V N G S NTG+ V S VD A +P S + TYS G+ Sbjct: 431 VA------AASPVVVNQG---SNNTGSTSVASIGVDSTYAGSPLASAVSLTYSGGS---- 477 Query: 358 LSPNPVGNVMVTTTAGVTTPYTPGTAIPYSAGATITADGVSFVLSGTPANNDKFVFSPTP 417 LS P G+V VT G T Y+ GTA PY+ GAT + +G+ L+GTPA ND F S Sbjct: 478 LSGFP-GSVTVTVN-GTATTYS-GTA-PYTQGATYSFNGIQMSLTGTPAANDTFTVSANT 533 Query: 418 ANT 420 AN+ Sbjct: 534 ANS 536 Score = 90.5 bits (223), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 99/336 (29%), Positives = 156/336 (46%), Gaps = 64/336 (19%) Query: 320 TSGNTGTGVVTSATVDKAAFAPNSTQTYTYSSGTNTLALSPNPVGNVMVTTTAGVTTPYT 379 +S NTGTG +T AT+ A YS G T++ P+ G V V++ Sbjct: 338 SSSNTGTGALT-ATITNANAGQGYDYQVKYSGGAYTVSHYPDGSGAVTVSS--------- 387 Query: 380 PGTAIPYSAGATITADGVSFVLSGTPANNDKFVFSPT----------------------- 416 P T DGV+ L+G+ A+ D F+ PT Sbjct: 388 ----WP------TTVDGVTLNLTGSMASGDSFLVRPTVNAASTMQTLTSDYHAVAAASPV 437 Query: 417 -----PANTGTATISSGTINSSFTATTLPAGSKLSFTFDSTTGTLATSPALGTAPMGTVT 471 NTG+ +++S ++S++ + P S +S T+ G+L+ P G+VT Sbjct: 438 VVNQGSNNTGSTSVASIGVDSTYAGS--PLASAVSLTYSG--GSLSGFP-------GSVT 486 Query: 472 VKDAGGNPVLVTPPNLPFGPGYSYTTSTGMSFSITGQPANGDQFYVSPNTGGVTDNRNAL 531 V G P+ G +Y+ + G+ S+TG PA D F VS NT TD NA Sbjct: 487 VTVNG--TATTYSGTAPYTQGATYSFN-GIQMSLTGTPAANDTFTVSANTANSTDGHNAS 543 Query: 532 ALNALQTANTI--GNTSFQGSYSQLVSLIGNKTNEITVTNKAENARLTAIQQEQQSESGV 589 A L+ A + G TS +S LV+ +G + ++ + ++ A L + +QQS SGV Sbjct: 544 AFAQLRNATVLDNGTTSISSGWSNLVTQVGIQASQASANLTSQKALLASSTSQQQSVSGV 603 Query: 590 NQDEELANMIKNQTAYQAAAKIIQAASDMLNVLFTL 625 N D+E N++K Q AYQA+AK++Q A+ + + L ++ Sbjct: 604 NLDDEAMNLMKYQQAYQASAKVMQTANSLFDSLLSI 639 >ZflgK [N] COG1256 Flagellar hook-associated protein EcZ Escherichia coli O157:H7 EDL933 Length = 547 Score = 201 bits (510), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 120/293 (40%), Positives = 170/293 (58%), Gaps = 11/293 (3%) Query: 13 QSLNDQMAQSRTSVNAQITSSIGSINSLATQIQELNQAIIKAQGSGGGAAPNDLLDQRDQ 72 Q L DQ Q VN I +S+ IN+ A QI LN I + G G GA+PN+LLDQRDQ Sbjct: 149 QYLRDQDKQ----VNIAIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQ 204 Query: 73 AIADLNKIVKVTTLPQNNGSMSVFIGNGQPLVLGDQVSQLVATPADDDPTRLQVSIKTSS 132 +++LN+IV V Q+ G+ ++ + NG LV G QL A P+ DP+R V+ + Sbjct: 205 LVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGT 264 Query: 133 NATILLPDGAFTGGSLGGILQYRTETLDTAQNTLGRVAIAMASAFNAQHKLGQDQNGALG 192 I +P+ GSLGGIL +R++ LD +NTLG++A+A A AFN+QHK G D NG G Sbjct: 265 AGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEG 324 Query: 193 TDFFNVPVPSVVANIKNHVALPVATATATISNPSALTTSDYRLDYDGFNYSVTRLSDNTK 252 DFF + P+V+ N KN+ + + AT+++ SA+ +DY++ +D + VTRL+ NT Sbjct: 325 EDFFAIGKPAVLQNTKNNGNVAIG---ATVTDASAVLATDYKISFDNNQWQVTRLASNTT 381 Query: 253 TT---SATMPVTIDGVDFGSAGMVA-KDSFLIKPTIDGANGISVAITNTQKIA 301 T A V DG++ G A DSF +KP D + V IT+ KIA Sbjct: 382 FTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIA 434 Score = 66.6 bits (161), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 49/151 (32%), Positives = 74/151 (49%), Gaps = 25/151 (16%) Query: 500 GMSFSITGQPANGDQFYVSP-----------------------NTGGVTDNRNALALNAL 536 G+ + TG PA D F + P G +DNRN AL L Sbjct: 396 GLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQALLDL 455 Query: 537 QT-ANTIGN-TSFQGSYSQLVSLIGNKTNEITVTNKAENARLTAIQQEQQSESGVNQDEE 594 Q+ + T+G SF +Y+ LVS IGNKT + ++ + +T + +QQS SGVN DEE Sbjct: 456 QSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSISGVNLDEE 515 Query: 595 LANMIKNQTAYQAAAKIIQAASDMLNVLFTL 625 N+ + Q Y A A+++Q A+ + + L + Sbjct: 516 YGNLQRFQQYYLANAQVLQTANAIFDALINI 546 >ECs1460 [N] COG1256 Flagellar hook-associated protein Ecs Escherichia coli O157:H7 Length = 547 Score = 201 bits (510), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 120/293 (40%), Positives = 170/293 (58%), Gaps = 11/293 (3%) Query: 13 QSLNDQMAQSRTSVNAQITSSIGSINSLATQIQELNQAIIKAQGSGGGAAPNDLLDQRDQ 72 Q L DQ Q VN I +S+ IN+ A QI LN I + G G GA+PN+LLDQRDQ Sbjct: 149 QYLRDQDKQ----VNIAIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQ 204 Query: 73 AIADLNKIVKVTTLPQNNGSMSVFIGNGQPLVLGDQVSQLVATPADDDPTRLQVSIKTSS 132 +++LN+IV V Q+ G+ ++ + NG LV G QL A P+ DP+R V+ + Sbjct: 205 LVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGT 264 Query: 133 NATILLPDGAFTGGSLGGILQYRTETLDTAQNTLGRVAIAMASAFNAQHKLGQDQNGALG 192 I +P+ GSLGGIL +R++ LD +NTLG++A+A A AFN+QHK G D NG G Sbjct: 265 AGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEG 324 Query: 193 TDFFNVPVPSVVANIKNHVALPVATATATISNPSALTTSDYRLDYDGFNYSVTRLSDNTK 252 DFF + P+V+ N KN+ + + AT+++ SA+ +DY++ +D + VTRL+ NT Sbjct: 325 EDFFAIGKPAVLQNTKNNGNVAIG---ATVTDASAVLATDYKISFDNNQWQVTRLASNTT 381 Query: 253 TT---SATMPVTIDGVDFGSAGMVA-KDSFLIKPTIDGANGISVAITNTQKIA 301 T A V DG++ G A DSF +KP D + V IT+ KIA Sbjct: 382 FTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIA 434 Score = 66.6 bits (161), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 49/151 (32%), Positives = 74/151 (49%), Gaps = 25/151 (16%) Query: 500 GMSFSITGQPANGDQFYVSP-----------------------NTGGVTDNRNALALNAL 536 G+ + TG PA D F + P G +DNRN AL L Sbjct: 396 GLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQALLDL 455 Query: 537 QT-ANTIGN-TSFQGSYSQLVSLIGNKTNEITVTNKAENARLTAIQQEQQSESGVNQDEE 594 Q+ + T+G SF +Y+ LVS IGNKT + ++ + +T + +QQS SGVN DEE Sbjct: 456 QSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSISGVNLDEE 515 Query: 595 LANMIKNQTAYQAAAKIIQAASDMLNVLFTL 625 N+ + Q Y A A+++Q A+ + + L + Sbjct: 516 YGNLQRFQQYYLANAQVLQTANAIFDALINI 546 >flgK [N] COG1256 Flagellar hook-associated protein Eco Escherichia coli K12 Length = 547 Score = 200 bits (509), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 120/293 (40%), Positives = 168/293 (57%), Gaps = 11/293 (3%) Query: 13 QSLNDQMAQSRTSVNAQITSSIGSINSLATQIQELNQAIIKAQGSGGGAAPNDLLDQRDQ 72 Q L DQ Q VN I +S+ IN+ A QI LN I + G G GA+PN+LLDQRDQ Sbjct: 149 QYLRDQDKQ----VNIAIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQ 204 Query: 73 AIADLNKIVKVTTLPQNNGSMSVFIGNGQPLVLGDQVSQLVATPADDDPTRLQVSIKTSS 132 +++LN+IV V Q+ G+ ++ + NG LV G QL A P+ DP+R V+ + Sbjct: 205 LVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGT 264 Query: 133 NATILLPDGAFTGGSLGGILQYRTETLDTAQNTLGRVAIAMASAFNAQHKLGQDQNGALG 192 I +P+ GSLGGIL +R++ LD +NTLG++A+A A AFN QHK G D NG G Sbjct: 265 AGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAG 324 Query: 193 TDFFNVPVPSVVANIKNHVALPVATATATISNPSALTTSDYRLDYDGFNYSVTRLSDNTK 252 DFF + P+V+ N KN + + AT+++ SA+ +DY++ +D + VTRL+ NT Sbjct: 325 EDFFAIGKPAVLQNTKNKGDVAIG---ATVTDASAVLATDYKISFDNNQWQVTRLASNTT 381 Query: 253 TT---SATMPVTIDGVDFGSAGMVA-KDSFLIKPTIDGANGISVAITNTQKIA 301 T A V DG++ G A DSF +KP D + V IT+ KIA Sbjct: 382 FTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIA 434 Score = 67.0 bits (162), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 49/151 (32%), Positives = 74/151 (49%), Gaps = 25/151 (16%) Query: 500 GMSFSITGQPANGDQFYVSP-----------------------NTGGVTDNRNALALNAL 536 G+ + TG PA D F + P G +DNRN AL L Sbjct: 396 GLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQALLDL 455 Query: 537 QT-ANTIGN-TSFQGSYSQLVSLIGNKTNEITVTNKAENARLTAIQQEQQSESGVNQDEE 594 Q+ + T+G SF +Y+ LVS IGNKT + ++ + +T + +QQS SGVN DEE Sbjct: 456 QSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSISGVNLDEE 515 Query: 595 LANMIKNQTAYQAAAKIIQAASDMLNVLFTL 625 N+ + Q Y A A+++Q A+ + + L + Sbjct: 516 YGNLQRFQQYYLANAQVLQTANAIFDALINI 546